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Information on EC 2.3.1.54 - formate C-acetyltransferase
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EC Tree
2.3.1.54 formate C-acetyltransferaseSpecify your search results
Word Map
- 2.3.1.54
-
glycyl
- anaerobiosis
- phosphotransacetylase
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chemostats
-
homolactic
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glucose-limited
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microaerobic
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pyruvate:ferredoxin
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mixed-acid
- acetoin
- flavodoxins
- activase
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5\'-deoxyadenosyl
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dual-phase
- hypophosphite
-
knappe
- benzylsuccinate
-
embden-meyerhof-parnas
- biotechnology
- nutrition
The enzyme appears in viruses and cellular organisms
Synonyms
pyruvate formate-lyase, pyruvate formate lyase, pyruvate-formate lyase, pyruvate:formate lyase, formate acetyltransferase, phosphotransferase system enzyme i, formate c-acetyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
formate acetyltransferase
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PFL
PFL I
PflB
pyruvate formate lyase
pyruvate formate-lyase
pyruvate-formate lyase
pyruvic formate-lyase
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PFL
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pyruvate formate lyase
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mutant strain CL3 with deletions in pflB, adhE, and frdA genes affecting D-lactate production
pyruvate formate-lyase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + formate = CoA + pyruvate
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acetyl-CoA + formate = CoA + pyruvate
modelling study proposes a four-step mechanism. The reaction is initiated by H transfer from C418 via C410 to the glycyl radical G734. H transfer from C419 to G734 is the rate-limiting process
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acetyl-CoA + formate = CoA + pyruvate
part of a bacterial-type mixed-acid fermentation in combination with alcohol dehydrogenase E
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acetyl-CoA + formate = CoA + pyruvate
active site structure includes an active site tunnel, substrate binding at Asp447 at the protein surface
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acetyl-CoA + formate = CoA + pyruvate
catalytic mechanism involving residues Gly734, Cys418 and Cys419, transition state, modeling, overview
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acetyl-CoA + formate = CoA + pyruvate
the reaction mechanism proceeds via generation of a catalytically essential glycyl radical at Gly734
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
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-
-
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:formate C-acetyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
9068-08-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + formate
CoA + pyruvate
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-
-
?
CoA + pyruvate
acetyl-CoA + formate
step in the mitochondrial pathway, overview
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?
CoA + pyruvate
acetyl-CoA + formate
anaerobic metabolism and potential ethanol-producing pathways in Chlamydomonas reinhardtii analyzed
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?
CoA + pyruvate
acetyl-CoA + formate
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the reaction involves Cys418 and Cys419
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?
CoA + pyruvate
acetyl-CoA + formate
mechanism to truncate an arginine-bound carboxylate motif, substrate mechanism of pyruvate formate-lyase used as a case study
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?
CoA + pyruvate
acetyl-CoA + formate
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anaerobic growth under nitrogen, 37°C, pH 7.0, extract enzyme reactions measured at room temperature
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?
CoA + pyruvate
acetyl-CoA + formate
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metabolic regulation and networking, overview
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?
CoA + pyruvate
acetyl-CoA + formate
Shewanella oneidensis MR-1 / ATCC 700550
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metabolic regulation and networking, overview
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?
CoA + pyruvate
acetyl-CoA + formate
formate is an important methyl group donor for anabolic pathway through the formation of folate derivates, formate supply improves Streptococcus thermophilus growth in milk
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?
CoA + pyruvate
acetyl-CoA + formate
formate is an important methyl group donor for anabolic pathway through the formation of folate derivates, formate supply improves Streptococcus thermophilus growth in milk
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?
pyruvate + CoA
acetyl-CoA + formate
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required for synthesis of C1 units in anabolism
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r
pyruvate + CoA
acetyl-CoA + formate
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required for synthesis of C1 units in anabolism
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?
pyruvate + CoA
acetyl-CoA + formate
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required for synthesis of C1 units in anabolism
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r
pyruvate + CoA
acetyl-CoA + formate
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physiological function is both anabolic and catabolic
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r
pyruvate + CoA
acetyl-CoA + formate
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required for synthesis of C1 units in anabolism
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?
pyruvate + CoA
acetyl-CoA + formate
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required for synthesis of C1 units in anabolism
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?
pyruvate + CoA
acetyl-CoA + formate
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required for synthesis of C1 units in anabolism
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r
pyruvate + CoA
acetyl-CoA + formate
required for synthesis of C1 units in anabolism
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?
pyruvate + CoA
acetyl-CoA + formate
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required for synthesis of C1 units in anabolism
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?
pyruvate + CoA
acetyl-CoA + formate
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no report on the reverse reaction
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ir, r
pyruvate + CoA
acetyl-CoA + formate
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catabolic function, leading to production of ATP
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?
pyruvate + CoA
acetyl-CoA + formate
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catabolic function, leading to production of ATP
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ir, r
pyruvate + CoA
acetyl-CoA + formate
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-
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?
pyruvate + CoA
acetyl-CoA + formate
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r
pyruvate + CoA
acetyl-CoA + formate
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reverse reaction with only 0.1% velocity of the forward reaction
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ir, r
pyruvate + CoA
acetyl-CoA + formate
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central reaction in the anaerobic metabolism
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r
pyruvate + CoA
acetyl-CoA + formate
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key enzyme of the glucose fermentation route in anaerobically growing cells
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?
pyruvate + CoA
acetyl-CoA + formate
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key enzyme of the glucose fermentation route in anaerobically growing cells
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r
pyruvate + CoA
acetyl-CoA + formate
key enzyme of the glucose fermentation route in anaerobically growing cells
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?
pyruvate + CoA
acetyl-CoA + formate
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acetyl-CoA for ATP synthesis in catabolism
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ir, r
pyruvate + CoA
acetyl-CoA + formate
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catabolic function, leading to production of ATP
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?
pyruvate + CoA
acetyl-CoA + formate
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catabolic function, leading to production of ATP
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ir, r
pyruvate + CoA
acetyl-CoA + formate
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main enzyme competing for pyruvate under anaerobic conditions
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r
pyruvate + CoA
acetyl-CoA + formate
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r
pyruvate + CoA
acetyl-CoA + formate
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catabolic function, leading to production of ATP
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?
pyruvate + CoA
acetyl-CoA + formate
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catabolic function, leading to production of ATP
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ir
pyruvate + CoA
formate + acetyl-CoA
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mixed-acid fermentation, necessary for growth in xylose minimal medium under anaerobic conditions
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
pyruvate + formate
formate + pyruvate
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carboxyl group exchange reaction
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r
additional information
?
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the enzyme is a glycyl radical enzyme, changes in the active site indicate that the actual substrate of PFL2 is bigger than a glycerol molecule, but sequence and structural homology suggest that PFL2 may be a dehydratase
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?
additional information
?
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phosphate cannot substitute for CoA
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?
additional information
?
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The product of yfiD gene is similar to pyruvate formate-lyase activase and it has been reported to activate PFL by replacing the glycyl radical domain, overview, the YfiD protein contributes to the pyruvate formate-lyase flux in the Escherichia coli arcA mutant strain, but not in the wild-type strain
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?
additional information
?
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pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation, molecular modeling of the FocA-PflB complex, the N-terminus of FocA is important for interaction with enzyme PflB
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?
additional information
?
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pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation, molecular modeling of the FocA-PflB complex, the N-terminus of FocA is important for interaction with enzyme PflB
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?
additional information
?
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formate channel FocA interacts with inactive PflB in vitro, analysis by mixing equal amounts of purified Strep-tagged FocA and purified His-tagged PflB, cross-links between FocA and PflBare identified, detailed overview
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?
additional information
?
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formate channel FocA interacts with inactive PflB in vitro, analysis by mixing equal amounts of purified Strep-tagged FocA and purified His-tagged PflB, cross-links between FocA and PflBare identified, detailed overview
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?
additional information
?
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pyruvate formate lyase (PFL) is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen
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?
additional information
?
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pyruvate formate lyase (PFL) is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen
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?
additional information
?
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hydrogen is formed from pyruvate by multiple parallel pathways, one pathway involves formate as an intermediate, pyruvate-formate lyase, and formate-hydrogen lyase, comprised of HydA hydrogenase and formate dehydrogenase, and a formate-independent pathway involving pyruvate dehydrogenase, the expression of pflB paralleled the expression of hydA and hyaB
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?
additional information
?
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Shewanella oneidensis MR-1 / ATCC 700550
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hydrogen is formed from pyruvate by multiple parallel pathways, one pathway involves formate as an intermediate, pyruvate-formate lyase, and formate-hydrogen lyase, comprised of HydA hydrogenase and formate dehydrogenase, and a formate-independent pathway involving pyruvate dehydrogenase, the expression of pflB paralleled the expression of hydA and hyaB
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?
additional information
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analysis of the cytosolic proteome involving the enzyme, proteome profile
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?
additional information
?
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analysis of the cytosolic proteome involving the enzyme, proteome profile
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?
additional information
?
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analysis of the cytosolic proteome involving the enzyme, proteome profile
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + formate
CoA + pyruvate
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?