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Literature summary for 2.3.1.54 extracted from
- Pyruvate formate-lyase of Escherichia coli: the acetyl-enzyme intermediate (1974), Eur. J. Biochem., 50, 253-263.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| formate | product inhibition | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0068 | - |
CoA | - |
Escherichia coli | |
| 0.051 | - |
acetyl-CoA | - |
Escherichia coli | |
| 2.05 | - |
pyruvate | - |
Escherichia coli | |
| 24 | - |
formate | - |
Escherichia coli | |
| 24.5 | - |
formate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + formate | Escherichia coli | - |
pyruvate + CoA | - |
r | |
| pyruvate + CoA | Escherichia coli | key role in the metabolism | acetyl-CoA + formate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| extreme sensitivity towards inactivation by oxygen, admittance of oxygen inactivates completely within a few seconds | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + formate | - |
Escherichia coli | pyruvate + CoA | - |
r | |
| pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | key role in the metabolism | Escherichia coli | acetyl-CoA + formate | - |
r | |
| pyruvate + dithiothreitol | - |
Escherichia coli | S-acetyl-dithiothreitol + formate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphotransferase system enzyme I | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
formate | - |
Escherichia coli | |
| 11 | - |
pyruvate | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.5 | forward direction | Escherichia coli |
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Release 2023.1 (January 2023)
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