Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Escherichia coli | |
Ferredoxin | - |
Clostridium butyricum | |
Ferredoxin | methylviologen can replace ferredoxin | Streptococcus mutans | |
oxamate | - |
Escherichia coli | |
oxamate | - |
Streptococcus mutans | |
S-adenosylmethionine | - |
Escherichia coli | |
S-adenosylmethionine | - |
Clostridium butyricum | |
S-adenosylmethionine | - |
Streptococcus mutans |
General Stability | Organism |
---|---|
unstable even when stored anaerobically | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Glyceraldehyde-3-phosphate | - |
Lactococcus lactis | |
D-Glyceraldehyde-3-phosphate | - |
Streptococcus mutans | |
dihydroxyacetone phosphate | - |
Lactococcus lactis | |
dihydroxyacetone phosphate | - |
Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
2 * 90000, SDS-PAGE | Streptococcus mutans |
170000 | 180000 | gel filtration | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA | Escherichia coli | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Lactococcus lactis | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Enterococcus faecalis | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Lactobacillus delbrueckii subsp. bulgaricus | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Clostridium butyricum | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Clostridium sp. | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Streptococcus mutans | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Streptococcus mutans JC2 | - |
acetyl-CoA + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium butyricum | - |
- |
- |
Clostridium sp. | - |
- |
- |
Enterococcus faecalis | - |
previously Streptococcus faecalis | - |
Escherichia coli | - |
- |
- |
Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
- |
Lactococcus lactis | - |
formerly designated Streptococcus lactis | - |
Streptococcus mutans | - |
JC2 | - |
Streptococcus mutans JC2 | - |
JC2 | - |
Oxidation Stability | Organism |
---|---|
immediately inactivated by exposure to the air | Streptococcus mutans |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Enterococcus faecalis |
- |
Streptococcus mutans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Streptococcus mutans |
Storage Stability | Organism |
---|---|
4°C, 50 mM potassium phosphate buffer, containing 1 mM dithiothreitol, storage of the purified enzyme in an anaerobic glove box for 2 weeks reduces activity by 50% | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Lactococcus lactis | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Enterococcus faecalis | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Lactobacillus delbrueckii subsp. bulgaricus | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Clostridium butyricum | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Clostridium sp. | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Streptococcus mutans | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Streptococcus mutans JC2 | acetyl-CoA + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | 2 * 90000, SDS-PAGE | Streptococcus mutans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 35 | activity is about 78 times of that at 22°C | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Clostridium butyricum |
7.5 | - |
- |
Streptococcus mutans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.3 | 40% of activity maximum at pH 6.5, between pH 7.0-8.3 80% of maximum activity | Streptococcus mutans |