Activating Compound | Comment | Organism | Structure |
---|---|---|---|
oxamate | obligatory component of the activation reaction | Escherichia coli | |
pyruvate | obligatory component of the activation reaction | Escherichia coli | |
S-adenosylmethionine | obligatory component of the activation reaction | Escherichia coli |
General Stability | Organism |
---|---|
virtually stable at 0°C and pH 8.0 if kept in media which display a redoxpotential of 0.2 V or below | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
CoA | - |
Escherichia coli | |
0.051 | - |
acetyl-CoA | - |
Escherichia coli | |
2 | - |
pyruvate | - |
Escherichia coli | |
24 | - |
formate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000 | Escherichia coli |
140000 | - |
gel filtration, sedimentation velocity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA | Enterococcus faecalis | - |
acetyl-CoA + formate | - |
r | |
pyruvate + CoA | Clostridium sp. | - |
acetyl-CoA + formate | - |
r | |
pyruvate + CoA | Escherichia coli | central reaction in the anaerobic metabolism | acetyl-CoA + formate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sp. | - |
- |
- |
Enterococcus faecalis | - |
previously Streptococcus faecalis | - |
Escherichia coli | - |
K12 | - |
Oxidation Stability | Organism |
---|---|
extreme sensitivity towards inactivation by oxygen, admittance of oxygen inactivates completely within a few seconds | Escherichia coli |
Purification (Comment) | Organism |
---|---|
partially | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable for at least several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + formate | - |
Escherichia coli | pyruvate + CoA | - |
r | |
pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
r | |
pyruvate + CoA | - |
Enterococcus faecalis | acetyl-CoA + formate | - |
r | |
pyruvate + CoA | - |
Clostridium sp. | acetyl-CoA + formate | - |
r | |
pyruvate + CoA | central reaction in the anaerobic metabolism | Escherichia coli | acetyl-CoA + formate | - |
r | |
pyruvate + formate | carboxyl group exchange reaction | Escherichia coli | formate + pyruvate | - |
r | |
pyruvate + formate | carboxyl group exchange reaction | Enterococcus faecalis | formate + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity declines with a half-time of 50 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.4 | - |
Escherichia coli |