Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | - |
Escherichia coli | |
S-adenosylmethionine | - |
Streptococcus mutans |
Cloned (Comment) | Organism |
---|---|
Escherichia coli SP 264 contains plasmid p29with pfl gene, identification of pfl gene product as a 80kDa polypeptide | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
identification of pfl gene product | Escherichia coli |
85000 | - |
2 * 85000, homodimer, SDS-PAGE | Escherichia coli |
85500 | - |
2 * 85500, homodimer, SDS-PAGE | Escherichia coli |
151000 | - |
sedimentation equilibrium | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA | Streptococcus mutans | - |
acetyl-CoA + formate | - |
? | |
pyruvate + CoA | Escherichia coli | key enzyme of the glucose fermentation route in anaerobically growing cells | acetyl-CoA + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Escherichia coli | - |
ATCC 10798 | - |
Streptococcus mutans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 10 mM MOPS-KOH, pH 7.0, 50 mM KCl, thiols, EDTA in 50% ethyleneglycol, proved stable for months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | - |
Streptococcus mutans | acetyl-CoA + formate | - |
? | |
pyruvate + CoA | key enzyme of the glucose fermentation route in anaerobically growing cells | Escherichia coli | acetyl-CoA + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Streptococcus mutans |
dimer | 2 * 85000, homodimer, SDS-PAGE | Escherichia coli |
dimer | 2 * 85500, homodimer, SDS-PAGE | Escherichia coli |