Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.54 extracted from

  • Thauer, R.K.; Kirchniawy, F.H.; Jungermann, K.A.
    Properties and function of the pyruvate-formate-lyase reaction in clostridiae (1972), Eur. J. Biochem., 27, 282-290.
    View publication on PubMed
Search for more literature for 2.3.1.54

Activating Compound

Activating Compound Comment Organism Structure
Ferredoxin
-
 Escherichia coli
Ferredoxin reactivation of inactive enzyme requires reduced ferredoxin, Fe2+-dithiol or Co2+-mercaptoethanol complexes can substitute for ferredoxin Clostridium butyricum
Ferredoxin reactivation of inactive enzyme requires reduced ferredoxin, Fe2+-dithiol or Co2+-mercaptoethanol complexes can substitute for ferredoxin Clostridium kluyveri
Ferredoxin reactivation of inactive enzyme requires reduced ferredoxin, Fe2+-dithiol or Co2+-mercaptoethanol complexes can substitute for ferredoxin Clostridium beijerinckii
S-adenosylmethionine obligatory component of the activation reaction Escherichia coli
S-adenosylmethionine obligatory component of the activation reaction Clostridium butyricum
S-adenosylmethionine obligatory component of the activation reaction Clostridium kluyveri
S-adenosylmethionine obligatory component of the activation reaction Clostridium beijerinckii

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Clostridium beijerinckii
ADP
-
 Clostridium butyricum
ADP
-
 Clostridium kluyveri
ATP regulates pyruvate cleavage to acetyl-CoA and formate, 10 mM reduces the reactin velocity by 95% Clostridium beijerinckii
ATP regulates pyruvate cleavage to acetyl-CoA and formate, 10 mM reduces the reactin velocity by 95% Clostridium butyricum
ATP regulates pyruvate cleavage to acetyl-CoA and formate, 10 mM reduces the reactin velocity by 95% Clostridium kluyveri
Cl-
-
 Clostridium beijerinckii
Cl-
-
 Clostridium butyricum
Cl-
-
 Clostridium kluyveri
CoA inhibits exchange reaction between pyruvate and formate, inhibits formate formation from pyruvate above 0.075 mM Clostridium beijerinckii
CoA inhibits exchange reaction between pyruvate and formate, inhibits formate formation from pyruvate above 0.075 mM Clostridium butyricum
CoA inhibits exchange reaction between pyruvate and formate, inhibits formate formation from pyruvate above 0.075 mM Clostridium kluyveri
DEAE-cellulose treated extracts do not catalyze formate formation from pyruvate Clostridium beijerinckii
DEAE-cellulose treated extracts do not catalyze formate formation from pyruvate Clostridium butyricum
DEAE-cellulose treated extracts do not catalyze formate formation from pyruvate Clostridium kluyveri
HPO42-
-
 Clostridium beijerinckii
HPO42-
-
 Clostridium butyricum
HPO42-
-
 Clostridium kluyveri
hypophosphite strongly inhibits formate formation from pyruvate Clostridium beijerinckii
hypophosphite strongly inhibits formate formation from pyruvate Clostridium butyricum
hypophosphite strongly inhibits formate formation from pyruvate Clostridium kluyveri
hypophosphite
-
 Escherichia coli
SO42-
-
 Clostridium beijerinckii
SO42-
-
 Clostridium butyricum
SO42-
-
 Clostridium kluyveri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 CoA pyruvate Clostridium butyricum
0.26
-
 acetyl-CoA
-
 Clostridium butyricum
0.6
-
 pyruvate pyruvate-formate exchange Clostridium butyricum
1.6
-
 pyruvate
-
 Clostridium butyricum
10
-
 formate
-
 Clostridium butyricum
20
-
 formate pyruvate-formate exchange Clostridium butyricum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates pyruvate cleavage about 2fold Clostridium butyricum
Mg2+ stimulates pyruvate cleavage about 2fold Clostridium kluyveri
Mg2+ stimulates pyruvate cleavage about 2fold Clostridium beijerinckii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA Clostridium sp.
-
 acetyl-CoA + formate
-
 r
pyruvate + CoA Clostridium butyricum required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 r
pyruvate + CoA Clostridium kluyveri required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 r
pyruvate + CoA Clostridium beijerinckii required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 r
pyruvate + CoA Clostridium butyricum physiological function is both anabolic and catabolic acetyl-CoA + formate
-
 r
pyruvate + CoA Escherichia coli acetyl-CoA for ATP synthesis in catabolism acetyl-CoA + formate
-
 ir
pyruvate + CoA Escherichia coli acetyl-CoA for ATP synthesis in catabolism acetyl-CoA + formate
-
 r
pyruvate + CoA Escherichia coli catabolic function, leading to production of ATP acetyl-CoA + formate
-
 ir
pyruvate + CoA Escherichia coli catabolic function, leading to production of ATP acetyl-CoA + formate
-
 r
pyruvate + CoA Enterococcus faecalis catabolic function, leading to production of ATP acetyl-CoA + formate
-
 ir
pyruvate + CoA Enterococcus faecalis catabolic function, leading to production of ATP acetyl-CoA + formate
-
 r
pyruvate + CoA Veillonella parvula catabolic function, leading to production of ATP acetyl-CoA + formate
-
 ir

Organism

Organism UniProt Comment Textmining
Clostridium beijerinckii
-
 ATCC 14823
-
Clostridium butyricum
-
 ATCC 19398
-
Clostridium kluyveri
-
-
-
Clostridium sp.
-
-
-
Enterococcus faecalis
-
 previously Streptococcus faecalis
-
Escherichia coli
-
-
-
no activity in Clostridium pasteurianum
-
 ATCC 6013
-
Veillonella parvula
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + CoA
-
 Clostridium butyricum propionyl-CoA + formate
-
 r
2-oxobutyrate + CoA
-
 Clostridium kluyveri propionyl-CoA + formate
-
 r
2-oxobutyrate + CoA
-
 Clostridium beijerinckii propionyl-CoA + formate
-
 r
acetyl-CoA + formate
-
 Escherichia coli pyruvate + CoA
-
 r
acetyl-CoA + formate
-
 Clostridium butyricum pyruvate + CoA
-
 r
acetyl-CoA + formate
-
 Clostridium kluyveri pyruvate + CoA
-
 r
acetyl-CoA + formate
-
 Clostridium beijerinckii pyruvate + CoA
-
 r
additional information
-
 Escherichia coli ?
-
 ?
additional information phosphate cannot substitute for CoA Clostridium butyricum ?
-
 ?
additional information phosphate cannot substitute for CoA Clostridium kluyveri ?
-
 ?
additional information phosphate cannot substitute for CoA Clostridium beijerinckii ?
-
 ?
pyruvate + CoA
-
 Veillonella parvula acetyl-CoA + formate
-
 ir
pyruvate + CoA
-
 Clostridium butyricum acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Clostridium kluyveri acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Clostridium sp. acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Clostridium beijerinckii acetyl-CoA + formate
-
 r
pyruvate + CoA reverse reaction with only 0.1% velocity of the forward reaction Escherichia coli acetyl-CoA + formate
-
 ir
pyruvate + CoA reverse reaction with only 0.1% velocity of the forward reaction Escherichia coli acetyl-CoA + formate
-
 r
pyruvate + CoA no report on the reverse reaction Enterococcus faecalis acetyl-CoA + formate
-
 ir
pyruvate + CoA no report on the reverse reaction Enterococcus faecalis acetyl-CoA + formate
-
 r
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium butyricum acetyl-CoA + formate
-
 r
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium kluyveri acetyl-CoA + formate
-
 r
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium beijerinckii acetyl-CoA + formate
-
 r
pyruvate + CoA physiological function is both anabolic and catabolic Clostridium butyricum acetyl-CoA + formate
-
 r
pyruvate + CoA acetyl-CoA for ATP synthesis in catabolism Escherichia coli acetyl-CoA + formate
-
 ir
pyruvate + CoA acetyl-CoA for ATP synthesis in catabolism Escherichia coli acetyl-CoA + formate
-
 r
pyruvate + CoA catabolic function, leading to production of ATP Escherichia coli acetyl-CoA + formate
-
 ir
pyruvate + CoA catabolic function, leading to production of ATP Escherichia coli acetyl-CoA + formate
-
 r
pyruvate + CoA catabolic function, leading to production of ATP Enterococcus faecalis acetyl-CoA + formate
-
 ir
pyruvate + CoA catabolic function, leading to production of ATP Enterococcus faecalis acetyl-CoA + formate
-
 r
pyruvate + CoA catabolic function, leading to production of ATP Veillonella parvula acetyl-CoA + formate
-
 ir
pyruvate + dephospho-CoA
-
 Clostridium butyricum dephospho-acetyl-CoA + formate
-
 r
pyruvate + dephospho-CoA
-
 Clostridium kluyveri dephospho-acetyl-CoA + formate
-
 r
pyruvate + dephospho-CoA
-
 Clostridium beijerinckii dephospho-acetyl-CoA + formate
-
 r
pyruvate + formate carboxyl group exchange reaction Escherichia coli formate + pyruvate
-
 r
pyruvate + formate carboxyl group exchange reaction Enterococcus faecalis formate + pyruvate
-
 r
pyruvate + formate carboxyl group exchange reaction Veillonella parvula formate + pyruvate
-
 r
pyruvate + formate carboxyl group exchange reaction Clostridium butyricum formate + pyruvate
-
 r
pyruvate + formate carboxyl group exchange reaction Clostridium kluyveri formate + pyruvate
-
 r
pyruvate + formate carboxyl group exchange reaction Clostridium beijerinckii formate + pyruvate
-
 r
pyruvate + phosphate
-
 Veillonella parvula acetylphosphate + formate
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 Tris-acetate buffer Clostridium butyricum
7 7.5 Tris-acetate buffer Clostridium kluyveri
7 7.5 Tris-acetate buffer Clostridium beijerinckii
7.5
-
-
 Clostridium butyricum
7.5
-
-
 Clostridium kluyveri
7.5
-
-
 Clostridium beijerinckii