Information on EC 3.5.1.14 - N-acyl-aliphatic-L-amino acid amidohydrolase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.5.1.14
-
RECOMMENDED NAME
GeneOntology No.
N-acyl-aliphatic-L-amino acid amidohydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate
show the reaction diagram
(2)
-
-
-
an N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
deacylation
-
-
-
-
hydrolysis
-
-
hydrolysis of amide bond
-
-
-
-
hydrolysis of N-acetylated amino acids
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine biosynthesis
-
-
arginine metabolism
-
-
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
N-acyl-aliphatic-L-amino acid amidohydrolase (carboxylate-forming)
Contains Zn2+. The enzyme is found in animals and is involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). It acts on mercapturic acids (S-conjugates of N-acetyl-L-cysteine) and neutral aliphatic N-acyl-alpha-amino acids. Some bacterial aminoacylases demonstrate substrate specificity of both EC 3.5.1.14 and EC 3.5.1.114. cf. EC 3.5.1.15, aspartoacylase and EC 3.5.1.114, N-acyl-aromatic-L-amino acid amidohydrolase.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-37-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
No.9
-
-
Manually annotated by BRENDA team
cow
-
-
Manually annotated by BRENDA team
Cercidium texanum
paloverde tree
-
-
Manually annotated by BRENDA team
strain Ax20
-
-
Manually annotated by BRENDA team
strain Ax20
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
ATCC 8014
-
-
Manually annotated by BRENDA team
strain MG1363
-
-
Manually annotated by BRENDA team
strain MG1363
-
-
Manually annotated by BRENDA team
avium, smegmatis, phlei
-
-
Manually annotated by BRENDA team
no activity in Streptomyces olivaceus
-
-
-
Manually annotated by BRENDA team
no activity in Streptomyces olivaceus IFO 12805
-
-
-
Manually annotated by BRENDA team
strain SE83
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gram-negative, motile, nonsporeforming rod-shaped bacterium, B1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
overexpression of aminoacylase 1 is associated with colorectal cancer progression. Enzyme knockdown inhibits cell proliferation and causes cell cycle perturbation
physiological function
-
the enzyme is responsible for amino acid deacylation during intracellular protein degradation, it is implicated in a number of human tumor types. Strong expression of the enzyme is significantly associated with more advanced TNM stage, lymph node metastasis, positive vascular invasion, and shorter cancer-specific survival
additional information
-
strong ACY1 immunoreactivity is an adverse prognostic indicator compared with combined negative and weak ACY 1 expression
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
show the reaction diagram
2-acetylamino-3-((1S,2S)-2-hydroxycyclooctylsulfanyl)-propionic acid + H2O
S-[(1S,2S)-2-hydroxycyclooctyl]cysteine + acetate
show the reaction diagram
-
-
-
-
?
2-acetylamino-3-((3S,4S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
show the reaction diagram
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
show the reaction diagram
3-(2-furyl)acryloyl-L-methionine + H2O
3-(2-furyl)acrylate + L-methionine
show the reaction diagram
-
-
-
-
?
8-methyl-N-vanillyl-6-nonenamide + H2O
?
show the reaction diagram
-
i.e. capsaicin
-
-
?
acetyl-DL-alloisoleucine + H2O
acetate + DL-alloisoleucine
show the reaction diagram
-
-
-
-
?
acetyl-DL-ethionine + H2O
acetate + DL-ethionine
show the reaction diagram
-
-
-
-
?
acetyl-DL-isoleucine + H2O
acetate + DL-isoleucine
show the reaction diagram
-
-
-
-
?
acetyl-L-beta amino acid + H2O
acetate + L-beta amino acid
show the reaction diagram
-
-
-
-
?
acetyl-L-methionine + H2O
acetate + L-methionine
show the reaction diagram
-
-
-
-
?
alpha,beta-dichloroacetyl-DL-diaminopropionic acid + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-DL-alanine + H2O
benzoate + DL-alanine
show the reaction diagram
-
-
-
-
?
benzoyl-DL-leucinamide + H2O
benzoate + DL-leucinamide
show the reaction diagram
-
-
-
-
?
benzoyl-DL-methionine + H2O
benzoate + DL-methionine
show the reaction diagram
-
-
-
-
?
benzoyl-DL-phenylalanine + H2O
benzoate + DL-phenylalanine
show the reaction diagram
benzoyl-DL-valine + H2O
benzoate + DL-valine
show the reaction diagram
-
-
-
-
?
benzoyl-L-leucine + H2O
benzoate + L-leucine
show the reaction diagram
-
-
-
-
?
benzoyloxycarbonglycyl-L-leucine + H2O
?
show the reaction diagram
-
-
-
-
?
bromoacetyl -DL_alanine + H2O
bromoacetate + DL-alanine
show the reaction diagram
-
-
-
-
?
bromoacetyl-DL-phenylalanine + H2O
bromoacetate + DL-phenylalanine
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid
show the reaction diagram
-
-
-
-
?
chloracetyl-DL-aminoheptylic acid + H2O
chloroacetate + DL-aminoheptanoate
show the reaction diagram
-
-
-
-
?
chloroacetyl-DL-allothreonine + H2O
chloroacetate + DL-allothreonine
show the reaction diagram
-
-
-
-
?
chloroacetyl-DL-serine + H2O
chloroacetate + DL-serine
show the reaction diagram
-
-
-
-
?
chloroacetyl-DL-threonine + H2O
chloroacetate + DL-threonine
show the reaction diagram
-
-
-
-
?
chloroacetyl-L-4-fluorophenylalanine + H2O
chloroacetate + L-4-fluorophenylalanine
show the reaction diagram
-
-
-
-
?
chloroacetyl-L-4-methoxyphenylalanine + H2O
chloroacetate + L-4-methoxyphenylalanine
show the reaction diagram
-
-
-
-
?
chloroacetyl-L-beta amino acid + H2O
chloroacetate + L-beta amino acid
show the reaction diagram
-
-
-
-
?
chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
show the reaction diagram
chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
dichloroacety-DL-phenylalanine + H2O
dichloroacetate + DL-phenylalanine
show the reaction diagram
-
-
-
-
?
dichloroacetyl-DL-alanine + H2O
dichloroacetate + DL-alanine
show the reaction diagram
-
-
-
-
?
dichloroacetyl-DL-ornithine + H2O
dichloroacetate + DL-ornithine
show the reaction diagram
-
-
-
-
?
DL-N-acetyl-D,L-3-(4-thiazolyl)alanine + H2O
L-3-(4-thiazolyl)alanine + D-N-acetyl-D-3-(4-thiazolyl)alanine
show the reaction diagram
-
-
-
-
-
fluoroacetyl-DL-alanine + H2O
fluoroacetate + DL-alanine
show the reaction diagram
-
-
-
-
?
fluoroacetyl-L-phenylalanine + H2O
fluoroacetate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
formyl-DL-alanine + H2O
formate + DL-alanine
show the reaction diagram
-
-
-
-
?
formyl-DL-phenylalanine + H2O
formate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
furylacryloyl-L-methionine + H2O
?
show the reaction diagram
-
-
-
-
?
glycyl-L-methionine + H2O
glycine + L-methionine
show the reaction diagram
-
-
-
-
?
glycyl-L-tyrosine + H2O
glycine + L-tyrosine
show the reaction diagram
-
-
-
-
?
hydroxyacetyl-DL-alanine + H2O
hydroxyacetate + DL-alanine
show the reaction diagram
-
isolated as the barium salt
-
-
?
hydroxyacetyl-DL-phenylalanine + H2O
hydroxyacetate + DL-phenylalanine
show the reaction diagram
-
-
-
-
?
iodoacetyl-DL-alanine + H2O
iodoacetate + DL-alanine
show the reaction diagram
-
-
-
-
?
iodoacetyl-DL-phenylalanine + H2O
iodoacetate + DL-phenylalanine
show the reaction diagram
-
-
-
-
?
L-methionylglycine + H2O
glycine + L-methionine
show the reaction diagram
-
-
-
-
?
m-nitrobenzoyl-DL-alanine + H2O
3-nitrobenzoate + DL-alanine
show the reaction diagram
-
-
-
-
?
methyl-mercaptoacetyl-DL-alanine + H2O
methylmercaptoacetate + DL-alanine
show the reaction diagram
-
-
-
-
?
methylmercaptoacetyl-DL-phenylalanine + H2O
methylmercaptoacetate + DL-phenylalanine
show the reaction diagram
-
-
-
-
?
N-(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
(E)-3-methyl-2-hexenoyl-glutamine + L-glutamine
show the reaction diagram
N-2,2-dimethylpropionyl-L-methionine + H2O
propionate + L-methionine
show the reaction diagram
-
-
-
?
N-3-methyl-3-hydroxy-hexanoyl-L-glutamine + H2O
3-methyl-3-hydroxy-hexanoate + L-glutamine
show the reaction diagram
N-acetyl-1,2-dichlorovinyl-L-cysteine + H2O
acetate + 1,2-dichlorovinyl-L-cysteine
show the reaction diagram
N-acetyl-D-Homophenylalanine + H2O
acetate + D-Homophenylalanine
show the reaction diagram
-
-
-
-
?
N-acetyl-DL-aspartate + H2O
acetate + DL-aspartate
show the reaction diagram
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
show the reaction diagram
N-acetyl-DL-methionine + H2O
N-acetyl-D-methionine + L-methionine + acetic acid
show the reaction diagram
-
-
-
-
-
N-acetyl-DL-norleucine + H2O
DL-norleucine + acetate
show the reaction diagram
N-acetyl-DL-proline + H2O
acetate + DL-proline
show the reaction diagram
-
-
-
-
?
N-acetyl-DL-serine + H2O
acetate + DL-serine
show the reaction diagram
-
-
-
-
?
N-acetyl-DL-Trp + H2O
acetate + DL-Trp
show the reaction diagram
-
-
-
-
-
N-acetyl-DL-tyrosine + H2O
acetate + DL-tyrosine
show the reaction diagram
N-Acetyl-Gly + H2O
acetate + Gly
show the reaction diagram
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
show the reaction diagram
N-acetyl-L-2-aminopentanoate + H2O
acetate + L-2-aminopentanoate
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Ala + H2O
acetate + L-Ala
show the reaction diagram
-
-
-
?
N-acetyl-L-alanine + H2O
acetate + L-alanine
show the reaction diagram
N-acetyl-L-alanine + H2O
L-alanine + acetate
show the reaction diagram
N-acetyl-L-amino acid + H2O
carboxylic acid + L-amino acid
show the reaction diagram
N-acetyl-L-Arg + H2O
acetate + L-Arg
show the reaction diagram
-
-
-
?
N-acetyl-L-arginine + H2O
acetate + L-arginine
show the reaction diagram
N-acetyl-L-Asn + H2O
acetate + L-Asn
show the reaction diagram
-
-
-
?
N-acetyl-L-Asp + H2O
acetate + L-Asp
show the reaction diagram
-
-
-
?
N-acetyl-L-asparagine + H2O
acetate + L-asparagine
show the reaction diagram
N-acetyl-L-aspartate + H2O
acetate + L-aspartate
show the reaction diagram
-
no activity of wild type protein, mutant E167R protein shows activity
-
-
?
N-acetyl-L-Cys + H2O
acetate + L-Cys
show the reaction diagram
N-acetyl-L-Cys + H2O
L-Cys + acetate
show the reaction diagram
-
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
show the reaction diagram
N-acetyl-L-ethionine + H2O
acetate + L-ethionine
show the reaction diagram
N-acetyl-L-Gln + H2O
acetate + L-Gln
show the reaction diagram
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
show the reaction diagram
N-acetyl-L-glutamine + H2O
acetate + L-glutamine
show the reaction diagram
N-acetyl-L-glutamine + H2O
L-glutamine + acetate
show the reaction diagram
N-acetyl-L-histidine + H2O
acetate + L-histidine
show the reaction diagram
N-acetyl-L-Homophenylalanine + H2O
acetate + L-Homophenylalanine
show the reaction diagram
-
-
-
-
?
N-acetyl-L-isoleucine + H2O
acetate + L-isoleucine
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Leu + H2O
acetate + L-Leu
show the reaction diagram
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
show the reaction diagram
N-acetyl-L-Met + H2O
acetate + L-Met
show the reaction diagram
N-acetyl-L-Met + H2O
L-Met + acetate
show the reaction diagram
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
show the reaction diagram
N-acetyl-L-methionine + H2O
acetate + L-methionine
show the reaction diagram
N-acetyl-L-methionine + H2O
acetic acid + L-methionine
show the reaction diagram
N-acetyl-L-norleucine + H2O
acetate + L-norleucine
show the reaction diagram
N-acetyl-L-ornithine + H2O
acetate + L-ornithine
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Phe + H2O
acetate + L-Phe
show the reaction diagram
-
-
-
?
N-acetyl-L-Phe methyl ester + H2O
acetate + L-Phe methyl ester
show the reaction diagram
-
the enzyme also shows esterase activity with N-acetyl-L-Phe methyl ester
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
show the reaction diagram
N-acetyl-L-phenylalanine + H2O
L-phenylalanine + acetate
show the reaction diagram
N-acetyl-L-phenylalanine methyl ester + H2O
acetate + L-phenylalanine methyl ester
show the reaction diagram
-
-
-
-
?
N-acetyl-L-serine + H2O
acetate + L-serine
show the reaction diagram
-
immobilised enzyme
-
-
?
N-acetyl-L-threonine + H2O
acetate + L-threonine
show the reaction diagram
N-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
show the reaction diagram
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
show the reaction diagram
N-acetyl-L-tyrosine + H2O
tyrosine + acetate
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Val + H2O
acetate + L-Val
show the reaction diagram
-
-
-
?
N-acetyl-L-Val + H2O
L-Val + acetate
show the reaction diagram
-
-
-
-
?
N-acetyl-L-valine + H2O
acetate + L-valine
show the reaction diagram
N-acetyl-L-valine + H2O
L-valine + acetate
show the reaction diagram
N-acetyl-O-benzyl-DL-serine + H2O
acetate + O-benzyl-DL-serine
show the reaction diagram
-
no hydrolysis with immobilized enzyme
-
-
?
N-acetyl-phi-DL-glycine + H2O
?
show the reaction diagram
-
only D-isomer hydrolyzed
-
-
?
N-acetyl-S-(1,1,2,2-tetrafluoroethyl)-L-cysteine + H2O
acetate + S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(1,2,2-trichlorovinyl)-L-cysteine + H2O
acetate + S-(1,2,2-trichlorovinyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine + H2O
acetate + S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine + H2O
acetate + S-(1,2-dichlorovinyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine + H2O
acetate + S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(2,2-dichlorovinyl)-L-cysteine + H2O
acetate + S-(2,2-dichlorovinyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(2,2-difluoro-1,1-dichloroethyl)-L-cysteine + H2O
acetate + S-(2,2-difluoro-1,1-dichloroethyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine + H2O
acetate + S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(2-chlorobenzyl)-L-cysteine + H2O
acetate + S-(2-chlorobenzyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(2-fluorobenzyl)-L-cysteine + H2O
acetate + S-(2-fluorobenzyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(3-fluorobenzyl)-L-cysteine + H2O
acetate + S-(3-fluorobenzyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(4-bromobenzyl)-L-cysteine + H2O
acetate + S-(4-bromobenzyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(4-chlorobenzyl)-L-cysteine + H2O
acetate + S-(4-chlorobenzyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-(4-methoxybenzyl)-L-cysteine + H2O
acetate + S-(4-methoxybenzyl)-L-cysteine
show the reaction diagram
-
-
-
?
N-acetyl-S-1,2-dichlorovinyl-L-cysteine + H2O
acetate + S-1,2-dichlorovinyl-L-cysteine
show the reaction diagram
-
-
-
-
?
N-acetyl-S-benzyl-L-cysteine + H2O
acetate + S-benzyl-L-cysteine
show the reaction diagram
N-acetyl-S-DL-benzylcysteine + H2O
acetate + S-DL-benzylcysteine
show the reaction diagram
-
-
-
-
?
N-acetylglycine + H2O
acetate + glycine
show the reaction diagram
N-acetyllysine + H2O
lysine + acetate
show the reaction diagram
-
-
-
-
?
N-acetylmethionine + H2O
acetate + L-methionine
show the reaction diagram
-
preferred substrate
-
-
?
N-acetylpipecolic acid + H2O
acetate + L-piperidine-2-carboxylate
show the reaction diagram
-
-
-
-
?
N-acetylseleno-L-methionine + H2O
acetate + L-selenomethionine
show the reaction diagram
-
-
-
-
?
N-acyl-L-amino acid + H2O
carboxylate + L-amino acid
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-alanine + H2O
acetate + L-alanine
show the reaction diagram
N-alpha-acetyl-L-aspartate + H2O
acetate + L-aspartate
show the reaction diagram
N-alpha-acetyl-L-cysteine + H2O
acetate + L-cysteine
show the reaction diagram
N-alpha-acetyl-L-glutamate + H2O
acetate + L-glutamate
show the reaction diagram
N-alpha-acetyl-L-isoleucine + H2O
acetate + L-isoleucine
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-leucine + H2O
acetate + L-leucine
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-lysine + H2O
acetate + L-lysine
show the reaction diagram
N-alpha-acetyl-L-methionine + H2O
acetate + L-methionine
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-proline + H2O
acetate + L-proline
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-serine + H2O
acetate + L-serine
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-threonine + H2O
acetate + L-threonine
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-tryptophan + H2O
acetate + L-tryptophan
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-L-valine + H2O
acetate + L-valine
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-phenylalanine + H2O
benzoate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-alpha-chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-arginine + H2O
benzoate + L-arginine
show the reaction diagram
-
immobilised enzyme
-
-
?
N-benzoyl-L-isoleucine + H2O
benzoate + L-isoleucine
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-leucine + H2O
benzoate + L-leucine
show the reaction diagram
-
immobilised enzyme
-
-
?
N-benzoyl-L-methionine + H2O
benzoate + L-methionine
show the reaction diagram
-
-
-
?
N-benzoyl-L-phenylalanine + H2O
benzoate + L-phenylalanine
show the reaction diagram
N-benzoyl-L-tert-leucine + H2O
benzoate + L-tert-isoleucine
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-threonine + H2O
benzoate + L-threonine
show the reaction diagram
-
immobilised enzyme
-
-
?
N-benzoyl-L-valine + H2O
benzoate + L-valine
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-L-Phe-Gly + H2O
benzyl hydrogencarbonate + L-Phe-Gly
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-L-phenylalanine + H2O
benzyl hydrogencarbonate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-L-phenylalanine + H2O
benzyloxycarbonate + L-phenylalanine
show the reaction diagram
-
33.9% of the activity with N-acetyl-L-phenylalanine. no activity with N-benzyloxycarbonyl-D-phenylalanine
-
-
?
N-benzyloxycarbonyl-L-valine + H2O
benzyl hydrogencarbonate + L-valine
show the reaction diagram
-
-
-
-
?
N-butanoyl-L-methionine + H2O
butanoate + L-methionine
show the reaction diagram
-
-
-
?
N-butyryl-D-aspartate + H2O
butanoate + D-aspartate
show the reaction diagram
-
-
-
-
?
N-butyryl-L-Met + H2O
butanoate + L-Met
show the reaction diagram
-
-
-
?
N-butyryl-L-methionine + H2O
butyrate + L-methionine
show the reaction diagram
-
-
-
?
N-carbobenzyloxy-L-glutamine + H2O
?
show the reaction diagram
N-CBZ-L-phenylalanine + H2O
? + L-phenylalanine
show the reaction diagram
-
immobilised enzyme
-
-
?
N-chloroacetyl-2-aminobutyrate + H2O
chloroacetate + 2-aminobutyrate
show the reaction diagram
-
-
-
-
?
N-chloroacetyl-chloroacetate + H2O
chloroacetate + acetate
show the reaction diagram
N-chloroacetyl-DL-alanine + H2O
chloroacetate + alanine
show the reaction diagram
N-chloroacetyl-DL-aspartate + H2O
chloroacetate + DL-aspartate
show the reaction diagram
-
-
-
-
?
N-chloroacetyl-DL-glutamine + H2O
chloroacetate + DL-glutamine
show the reaction diagram
-
-
-
-
?
N-chloroacetyl-DL-norvaline + H2O
chloroacetate + DL-norvaline
show the reaction diagram
-
N-acylated racemic amino acids, pure acylated D forms hydrolyzed 10000-40000 times more slowly than corresponding pure acylated L isomers
-
-
?
N-chloroacetyl-DL-phenylalanine + H2O
chloroacetate + phenylalanine
show the reaction diagram
N-chloroacetyl-DL-proline + H2O
chloroacetate + DL-proline
show the reaction diagram
-
-
-
-
?
N-chloroacetyl-DL-tyrosine + H2O
chloroacetate + DL-tyrosine
show the reaction diagram
-
-
-
-
?
N-chloroacetyl-DL-valine + H2O
chloroacetate + DL-valine
show the reaction diagram
N-chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
show the reaction diagram
N-chloroacetyl-L-methionine + H2O
chloroacetate + L-methionine
show the reaction diagram
N-chloroacetyl-L-phenylalanine + H2O
chloroacetate + L-phenylalanine
show the reaction diagram
N-chloroacetylaminocaprylate + H2O
chloroacetate + aminocaprylate
show the reaction diagram
-
-
-
-
?
N-chloroacetylaminocyclohexylacetate + H2O
chloroacetate + aminocyclohexylacetate
show the reaction diagram
-
-
-
-
?
N-chloroacetylaminocyclohexylbutyrate + H2O
chloroacetate + aminocyclohexylbutyrate
show the reaction diagram
-
-
-
-
?
N-chloroacetylaminocyclohexylpropionate + H2O
chloroacetate + aminocyclohexylpropionate
show the reaction diagram
-
-
-
-
?
N-chloroacetylaminophenylacetate + H2O
chloroacetate + aminophenylacetate
show the reaction diagram
-
-
-
-
?
N-chloroacetylglutamine-p-aminobenzoate + H2O
?
show the reaction diagram
N-chloroacetylglycine + H2O
chloroacetate + glycine
show the reaction diagram
N-chloroacetyltryptophan + H2O
chloroacetate + tryptophan
show the reaction diagram
N-cyclohexanoyl-L-methionine + H2O
hexanoate + L-methionine
show the reaction diagram
-
-
-
?
N-cyclopentanoyl-L-methionine + H2O
pentanoate + L-methionine
show the reaction diagram
-
-
-
?
N-decanoyl-L-glutamine + H2O
decanoate + L-glutamine
show the reaction diagram
N-decanoyl-L-Met + H2O
decanoate + L-Met
show the reaction diagram
-
-
-
?
N-decanoyl-L-methionine + H2O
decanoate + L-methionine
show the reaction diagram
-
-
-
?
N-dichloroacetyl-DL-lysine + H2O
dichloroacetate + DL-lysine
show the reaction diagram
-
-
-
-
?
N-dichloroacetylnorleucine + H2O
dichloroacetate + norleucine
show the reaction diagram
-
-
-
-
?
N-formyl-D-aspartate + H2O
formate + D-aspartate
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
show the reaction diagram
N-formylmethionine + H2O
formate + L-methionine
show the reaction diagram
-
substrate synthesis and purification, overview
-
-
?
N-glycyl-D-aspartate + H2O
glycine + D-aspartate
show the reaction diagram
-
-
-
-
?
N-hexanoyl-L-Met + H2O
hexanoate + L-Met
show the reaction diagram
-
-
-
?
N-hexanoyl-L-methionine + H2O
hexanoate + L-methionine
show the reaction diagram
N-isobutanoyl-L-methionine + H2O
isobutanoate + L-methionine
show the reaction diagram
-
-
-
?
N-isopentanoyl-L-methionine + H2O
pentanoate + L-methionine
show the reaction diagram
-
-
-
?
N-lauroyl-Gly + H2O
laurate + Gly
show the reaction diagram
-
-
-
?
N-lauroyl-glycine + H2O
laurate + glycine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-Ala + H2O
laurate + L-Ala
show the reaction diagram
-
-
-
?
N-lauroyl-L-alanine + H2O
laurate + L-alanine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-Arg + H2O
laurate + L-Arg
show the reaction diagram
-
-
-
?
N-lauroyl-L-arginine + H2O
laurate + L-arginine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-Asn + H2O
laurate + L-Asn
show the reaction diagram
-
-
-
?
N-lauroyl-L-asparagine + H2O
laurate + L-asparagine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-aspartate + H2O
laurate + L-aspartate
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-Cys + H2O
laurate + L-Cys
show the reaction diagram
-
-
-
?
N-lauroyl-L-glutamate + H2O
laurate + L-glutamate
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-glutamine + H2O
laurate + L-glutamine
show the reaction diagram
N-lauroyl-L-glutaminyl-glycine + H2O
laurate + L-glutaminyl-glycine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-glutaminyl-L-lysine + H2O
laurate + L-glutaminyl-L-lysine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-glutamyl-glycine + H2O
laurate + L-glutamyl-glycine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-His + H2O
laurate + L-His
show the reaction diagram
-
-
-
?
N-lauroyl-L-histidine + H2O
laurate + L-histidine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-isoleucine + H2O
laurate + L-isoleucine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-leucine + H2O
laurate + L-leucine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-Lys + H2O
laurate + L-Lys
show the reaction diagram
-
-
-
?
N-lauroyl-L-Met + H2O
laurate + L-Met
show the reaction diagram
-
-
-
?
N-lauroyl-L-methionine + H2O
laurate + L-methionine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-phenylalanine + H2O
laurate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-Ser + H2O
laurate + L-Ser
show the reaction diagram
-
-
-
?
N-lauroyl-L-serine + H2O
laurate + L-serine
show the reaction diagram
-
best substrate
-
-
?
N-lauroyl-L-threonine + H2O
laurate + L-threonine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-tryptophan + H2O
laurate + L-tryptophan
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-tyrosine + H2O
laurate + L-tyrosine
show the reaction diagram
-
-
-
-
?
N-lauroyl-L-valine + H2O
laurate + L-valine
show the reaction diagram
-
-
-
-
?
N-lauryl-glycine + H2O
laurate + glycine
show the reaction diagram
-
-
-
?
N-lauryl-L-alanine + H2O
laurate + L-alanine
show the reaction diagram
-
-
-
?
N-lauryl-L-cysteine + H2O
laurate + L-cysteine
show the reaction diagram
-
-
-
?
N-lauryl-L-lysine + H2O
laurate + L-lysine
show the reaction diagram
-
-
-
?
N-lauryl-L-methionine + H2O
laurate + L-methionine
show the reaction diagram
-
-
-
?
N-lauryl-L-serine + H2O
laurate + L-serine
show the reaction diagram
-
-
-
?
N-linolenoyl-L-glutamine + H2O
linolenic acid + L-glutamine
show the reaction diagram
-
-
-
?
N-myristoyl-L-Met + H2O
myristate + L-Met
show the reaction diagram
-
-
-
?
N-myristoyl-L-methionine + H2O
myristate + L-methionine
show the reaction diagram
-
-
-
?
N-octanoyl-L-Met + H2O
octanoate + L-Met
show the reaction diagram
-
-
-
?
N-octanoyl-L-methionine + H2O
octanoate + L-methionine
show the reaction diagram
-
-
-
?
N-palmitoyl-L-Met + H2O
palmitate + L-Met
show the reaction diagram
-
-
-
?
N-palmitoyl-L-methionine + H2O
palmitate + L-methionine
show the reaction diagram
-
-
-
?
N-pentanoyl-L-methionine + H2O
pentanoate + L-methionine
show the reaction diagram
-
-
-
?
N-propionyl-D-aspartate + H2O
proipionate + D-aspartate
show the reaction diagram
-
-
-
-
?
N-propionyl-L-methionine + H2O
propionate + L-methionine
show the reaction diagram
-
-
-
?
N-t-BOC-L-phenylalanine + H2O
? + L-phenylalanine
show the reaction diagram
-
immobilised enzyme
-
-
?
N-tert-butyloxycarbonyl-L-phenylalanine + H2O
N-tert-butyl hydrogencarbonate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
Nalpha-acetylmethionine + H2O
acetate + L-methionine
show the reaction diagram
-
-
-
-
?
Nalpha-lauroyl-L-lysine + H2O
laurate + L-lysine
show the reaction diagram
-
-
-
-
?
Nepsilon-lauroyl-L-lysine + H2O
laurate + L-lysine
show the reaction diagram
-
-
-
-
?
O-methyl-N-acetyl-DL-serine + H2O
acetate + O-methyl-DL-serine
show the reaction diagram
-
-
-
-
?
p-bromobenzoyl-DL-alanine + H2O
4-bromobenzoate + DL-alanine
show the reaction diagram
-
-
-
-
?
p-chlorobenzoyl-DL-alanine + H2O
4-chlorbenzoate + DL-alanine
show the reaction diagram
-
-
-
-
?
p-fluorobenzoyl-DL-alanine + H2O
4-fluorobenzoate + DL-alanine
show the reaction diagram
-
-
-
-
?
p-iodobenzoyl-DL-alanine + H2O
4-iodobenzoate + DL-alanine
show the reaction diagram
-
-
-
-
?
p-nitrobenzoyl-DL-alanine + H2O
4-nitrobenzoate + DL-alanine
show the reaction diagram
-
-
-
-
?
p-tolyl-DL-alanine + H2O
?
show the reaction diagram
-
-
-
-
?
palmitoyl-L-aspartate + H2O
palmitate + L-aspartate
show the reaction diagram
-
-
-
-
?
palmitoyl-L-phenylalanine + H2O
palmitate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
propionyl-DL-alanine + H2O
propanoate + DL-alanine
show the reaction diagram
-
-
-
-
?
S-(1,1,2,2-tetrafluoroethyl)-N-acetyl-L-cysteine + H2O
acetate + S-(-1,1,2,2-terafluoroethyl)-L-cysteine
show the reaction diagram
-
-
-
-
?
S-(2-bromo-1,1,2-trifluoroethyl)-N-acetyl-L-cysteine + H2O
acetate + S-(2-bromo-1,1,2-trifluoroethyl)-L-cysteine
show the reaction diagram
-
-
-
-
?
S-(2-chloro-1,1,2-trifluoroethyl)-N-acetyl-L-cysteine + H2O
acetate + S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
show the reaction diagram
-
-
-
-
?
S-ethyl-N-acetyl-L-cysteine + H2O
acetate + S-ethyl-L-cysteine
show the reaction diagram
-
-
-
-
?
S-isobutyl-N-acetyl-L-cysteine + H2O
?
show the reaction diagram
-
-
-
-
?
S-isopropyl-N-acetyl-L-cysteine + H2O
acetate + S-isopropyl-L-cysteine
show the reaction diagram
-
-
-
-
?
S-methyl-N-acetyl-L-cysteine + H2O
acetate + S-methyl-L-cysteine
show the reaction diagram
-
-
-
-
?
S-n-butyl-N-acetyl-L-cysteine + H2O
acetate + S-n-butyl-L-cysteine
show the reaction diagram
-
-
-
-
?
S-n-propyl-N-acetyl-L-cysteine + H2O
acetate + S-n-propyl-L-cysteine
show the reaction diagram
-
-
-
-
?
trifluoroacetyl-DL-alanine + H2O
trifluoroacetate + DL-alanine
show the reaction diagram
-
trifluoroacetyl derivatives are most rapidly hydrolyzed
-
-
?
trifluoroacetyl-DL-phenylalanine + H2O
trifluoroacetate + DL-phenylalanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetyl-1,2-dichlorovinyl-L-cysteine + H2O
acetate + 1,2-dichlorovinyl-L-cysteine
show the reaction diagram
N-acetyl-L-alanine + H2O
acetate + L-alanine
show the reaction diagram
C9K2Z6
-
-
-
?
N-acetyl-L-amino acid + H2O
carboxylic acid + L-amino acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
show the reaction diagram
C9K2Z6
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
show the reaction diagram
N-acetyl-L-methionine + H2O
acetate + L-methionine
show the reaction diagram
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CuCl2
-
relative activity of ACY is 22.9% when dipicolinic acid is removed; relative activity of ACY is 26.4% when EDTA is removed
MnCl2
-
relative activity of ACY is 125.3% when dipicolinic acid is removed; relative activity of ACY is 203.5% when EDTA is removed
ZnCl2
-
relative activity of ACY is 113.7% when EDTA is removed; relative activity of ACY is 90.8% when dipicolinic acid is removed
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
1,3-dichloro-2-propanone
-
-
1-chloro-2-tosylamido-7-amino-2-heptanone
-
25 mM, 7% inhibition
-
2,3-Butanedione
-
-
2-mercaptoethanol
20% inhibition at 1 mM; 81% residual activity at 1 mM
4-hydroxymercuribenzoate
-
1 mM, 67% inhibition
Ba2+
-
0.01 mM, 17% inhibition
benzamidine
-
0.1 mM, 7% inhibition
benzyloxycarbonyl-Gly-Gly-Phe
-
beta-mercaptoethanol
Cs+
-
0.01 mM, 47% inhibition
D-phenylalanine
-
inhibition of benzoyl-L-leucine and benzoyloxycarbonglycyl-L-leucine hydrolysis
diethyldicarbonate
diethyldithiocarbamate
-
-
Diethylpyrocarbonate
-
-
dithiothreitol
DL-lactate
Fe3+
-
0.01 mM, 99% inhibition
iodoacetamide
iodoacetate
-
1 mM, 1.1fold activation
iodoacetic acid
Isocaproic acid
-
-
Isopropanol
-
10%
K+
-
0.01 mM, 24% inhibition
L-alpha-fluoro-beta-phenyl-propionate
-
-
L-benzylsuccinate
L-beta-Phenyllactate
La3+
-
0.01 mM, 17% inhibition
Li+
-
0.01 mM, 51% inhibition
mono-tert-butyl malonate
-
25 mM, 14% inhibition
N-(4-toluenesulfonyl)-DL-methionine
-
25 mM, 14% inhibition
-
N-(p-toluenesulfonyl)-DL-Met
-
25 mM, 14% inhibition
-
N-acetyl-L-2-aminohexanoic acid
-
-
-
N-alpha-p-Tosyl-L-lysine
-
-
n-butylmalonic acid
-
-
N-ethylmaleimide
-
-
Na+
-
0.01 mM, 19% inhibition
Nepsilon-formyl-Nepsilon-hydroxy-L-lysine
-
IC50: 1.0 mM
nitrilotriacetate
-
-
p-chloromercuribenzoate
PCMB
-
inhibits murine AAIII
Phenanthroline
-
phenylboronic acid
-
-
phenylmethylsulfonyl fluoride
-
1 mM, 22% inhibition
Sn4+
-
0.01 mM, 99% inhibition
Sodium arsenite
-
higher inhibitory effect on L-aminacylase
Tris-(2-amino-ethyl)-amine
-
-
Vinylpyridine
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithioerythritol
-
with palmitoylaspartate as substrate
dithiothreitol
-
slight activation of short acyl aminoacylase at 0.2 mM
DTT
-
activates murine AAIII
mercaptoethanol
-
-
mono-benzyl malonate
-
25 mM, activation to 109% of control
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1 - 0.2
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
0.3 - 0.5
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
1
2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37C
0.2 - 0.4
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
0.22
furylacryloyl-L-methionine
-
-
0.2
N-(E)-3-methyl-2-hexenoyl-L-glutamine
-
pH 7
168 - 585
N-2,2-dimethylpropionyl-L-methionine
0.74
N-3-methyl-3-hydroxy-hexanoyl-L-glutamine
-
pH 7
2.52
N-acetyl-D-aspartate
-
-
12.7
N-acetyl-DL-aspartate
-
-
43.6 - 59.9
N-acetyl-glycine
2.32 - 20.1
N-acetyl-L-alanine
34.8
N-acetyl-L-arginine
-
25C, pH 7.4
27.7
N-acetyl-L-asparagine
-
25C, pH7.4
0.4 - 0.5
N-acetyl-L-Cys
1.4 - 4.4
N-acetyl-L-cysteine
1.28 - 1.87
N-acetyl-L-ethionine
63 - 102
N-acetyl-L-glutamate
5.9
N-acetyl-L-glutamic acid
-
pH 7.0, 30C
7 - 62.3
N-acetyl-L-glutamine
1.8 - 22.7
N-acetyl-L-histidine
6.72
N-acetyl-L-isoleucine
-
25C, pH 7.4
2.4 - 3.8
N-acetyl-L-leucine
0.2 - 11.3
N-acetyl-L-Met
0.013 - 24.6
N-acetyl-L-methionine
0.44 - 1.57
N-acetyl-L-norleucine
1.6 - 10.3
N-acetyl-L-phenylalanine
30.9 - 32.5
N-acetyl-L-threonine
1.2
N-acetyl-L-tryptophan
-
37C, pH 7.5
1.4
N-acetyl-L-tyrosine
-
37C, pH 7.5
0.4 - 0.5
N-acetyl-L-Val
2.5 - 16.6
N-acetyl-L-valine
0.25
N-acetyl-S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
-
37C, pH 7.5
1.2
N-acetyl-S-(1,2,2-trichlorovinyl)-L-cysteine
-
37C, pH 7.5
1
N-acetyl-S-(1,2,3,4,4-pentachlorobutadienyl)-L-cysteine
-
37C, pH 7.5
0.9
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine
-
37C, pH 7.5
5.3
N-acetyl-S-(2,2-dichloro-1,1-difluoroethyl)-L-cysteine
-
37C, pH 7.5
0.4
N-acetyl-S-(2,2-dichlorovinyl)-L-cysteine
-
37C, pH 7.5
0.3
N-acetyl-S-(2,2-difluoro-1,1-dichloroethyl)-L-cysteine
-
37C, pH 7.5
0.28
N-acetyl-S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
-
37C, pH 7.5
1.45
N-acetyl-S-(2-chlorobenzyl)-L-cysteine
-
37C, pH 7.5
1.3
N-acetyl-S-(2-fluorobenzyl)-L-cysteine
-
37C, pH 7.5
0.6
N-acetyl-S-(3-fluorobenzyl)-L-cysteine
-
37C, pH 7.5
0.5
N-acetyl-S-(4-bromobenzyl)-L-cysteine
-
37C, pH 7.5
0.3
N-acetyl-S-(4-chlorobenzyl)-L-cysteine
-
37C, pH 7.5
0.8
N-acetyl-S-(4-methoxybenzyl)-L-cysteine
-
37C, pH 7.5
1.1
N-acetyl-S-benzyl-L-cysteine
-
37C, pH 7.5
0.3
N-acetylmethionine
-
pH 8.0, 22C
4
N-acetylseleno-L-methionine
-
-
1.2
N-alpha-acetyl-L-alanine
-
pH 7.0, 30C
5
N-alpha-acetyl-L-aspartate
-
pH 7.0, 30C
9.1
N-alpha-acetyl-L-cysteine
-
pH 7.0, 30C
3.1
N-alpha-acetyl-L-isoleucine
-
pH 7.0, 30C
2.3
N-alpha-acetyl-L-leucine
-
pH 7.0, 30C
1.3 - 6.7
N-alpha-acetyl-L-lysine
0.84 - 7.7
N-alpha-acetyl-L-methionine
1.7 - 2.7
N-alpha-acetyl-L-phenylalanine
5
N-alpha-acetyl-L-proline
-
pH 7.0, 30C
4.2
N-alpha-acetyl-L-serine
-
pH 7.0, 30C
4
N-alpha-acetyl-L-threonine
-
pH 7.0, 30C
2.4
N-alpha-acetyl-L-tryptophan
-
pH 7.0, 30C
1.9
N-alpha-acetyl-L-tyrosine
-
pH 7.0, 30C
2.5
N-alpha-acetyl-L-valine
-
pH 7.0, 30C
1.4
N-alpha-chloroacetyl-L-phenylalanine
-
pH 7.0, 30C
3.71
N-benzoyl-L-glutamate
-
hydrolysis of N-acetyl-L-glutamate substrates by Wild-Type porcine Acy1
0.38 - 1.63
N-benzoyl-L-methionine
4 - 9.7
N-benzoyl-L-phenylalanine
5 - 10.7
N-benzoyl-L-valine
0.31 - 0.73
N-butanoyl-L-methionine
0.033
N-butyryl-D-aspartate
-
-
4.3
N-CBZ-Gly-Ala
-
-
0.31 - 6.23
N-cyclohexanoyl-L-methionine
0.64 - 7.18
N-cyclopentanoyl-L-methionine
0.08
N-decanoyl-L-glutamine
-
pH 7
12.5
N-formyl-D-aspartate
-
-
16.8
N-formyl-L-methionine
-
hydrolysis of N-acyl-L-methionine substrates by hAcy1
35.4
N-formyl-Lmethionine
-
hydrolysis of N-acyl-L-methionine substrates by pAcy1
3.1
N-formylmethionine
-
pH 8.0, 22C
1.11
N-glycyl-D-aspartate
-
-
0.01 - 5.22
N-hexanoyl-L-methionine
15.3 - 58.5
N-isobutanoyl-L-methionine
0.54 - 7.29
N-isopentanoyl-L-methionine
0.06
N-lauroyl-L-glutamine
-
pH 7
0.03 - 0.19
N-pentanoyl-L-methionine
0.194
N-propionyl-D-aspartate
-
-
0.34 - 0.55
N-propionyl-L-methionine
0.05
N2-[(benzyloxy)carbonyl]-L-glutamine
-
pH 7
0.39 - 67.4
Nalpha-acetylmethionine
0.15
palmitoylaspartate
-
-
3.4
S-ethyl-N-acetyl-L-cysteine
-
-
2.1
S-methyl-N-acetyl-L-cysteine
-
-
3.7
S-n-butyl-N-acetyl-L-cysteine
-
-
0.8
S-n-propyl-N-acetyl-L-cysteine
-
-
additional information
additional information