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Literature summary for 3.5.1.14 extracted from

  • Uttamsingh, V.; Keller, D.A.; Anders, M.W.
    Acylase I-catalyzed deacetylatin of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines (1998), Chem. Res. Toxicol., 11, 801-809.
    View publication on PubMed
Search for more literature for 3.5.1.14

Application

Application Comment Organism
medicine N-acetyl-L-cysteine used for drugs against lung disorders and HIV infections, test of potential antitumor agents Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
N-acetyl-L-2-aminohexanoic acid
-
 Rattus norvegicus
n-butylmalonic acid
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 S-n-propyl-N-acetyl-L-cysteine
-
 Rattus norvegicus
2.1
-
 N-acetyl-L-methionine
-
 Rattus norvegicus
2.1
-
 S-methyl-N-acetyl-L-cysteine
-
 Rattus norvegicus
3.4
-
 S-ethyl-N-acetyl-L-cysteine
-
 Rattus norvegicus
3.7
-
 S-n-butyl-N-acetyl-L-cysteine
-
 Rattus norvegicus
4
-
 N-acetylseleno-L-methionine
-
 Rattus norvegicus
4.4
-
 N-acetyl-L-cysteine
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Sus scrofa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, SDS-PAGE Rattus norvegicus
43000
-
 2 * 43000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-methionine Homo sapiens
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Rattus norvegicus
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
 rat, male Sprague-Dawley
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
endothelium
-
 Homo sapiens
-
intestinal mucosa
-
 Rattus norvegicus
-
intestinal mucosa
-
 Sus scrofa
-
kidney
-
 Rattus norvegicus
-
kidney
-
 Sus scrofa
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-methionine + H2O
-
 Homo sapiens DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Rattus norvegicus DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
N-acetyl-L-2-aminopentanoate + H2O
-
 Rattus norvegicus acetate + L-2-aminopentanoate
-
 ?
N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + L-cysteine
-
 ?
N-acetyl-L-methionine
-
 Homo sapiens L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Rattus norvegicus L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
N-acetylseleno-L-methionine + H2O
-
 Rattus norvegicus acetate + L-selenomethionine
-
 ?
O-methyl-N-acetyl-DL-serine + H2O
-
 Rattus norvegicus acetate + O-methyl-DL-serine
-
 ?
S-(1,1,2,2-tetrafluoroethyl)-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-(-1,1,2,2-terafluoroethyl)-L-cysteine
-
 ?
S-(2-bromo-1,1,2-trifluoroethyl)-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-(2-bromo-1,1,2-trifluoroethyl)-L-cysteine
-
 ?
S-(2-chloro-1,1,2-trifluoroethyl)-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
-
 ?
S-ethyl-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-ethyl-L-cysteine
-
 ?
S-isobutyl-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus ?
-
 ?
S-isopropyl-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-isopropyl-L-cysteine
-
 ?
S-methyl-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-methyl-L-cysteine
-
 ?
S-n-butyl-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-n-butyl-L-cysteine
-
 ?
S-n-propyl-N-acetyl-L-cysteine + H2O
-
 Rattus norvegicus acetate + S-n-propyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Rattus norvegicus
dimer 2 * 43000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sus scrofa