EC Number   |
|---|
    3.5.1.14 | - |
| 3.5.1.14 | ACYI, DNA and amino acid sequence determination and analysis, comparison with the porcine intestinal enzyme |
| 3.5.1.14 | ACYI, DNA and amino acid sequence determination and analysis, comparison with the rat kidney enzyme |
| 3.5.1.14 | cloning of Acy1 from a mouse kidney library, expression of the C-terminal fragment in HEK-293 cells, in vitro expression of the GST-tagged C-terminal fragment, expression as myc-tagged enzyme in Cos-7 cells, expression with murine sphingosine kinase type 1, SphK1, in a yeast two-hybrid system |
| 3.5.1.14 | DNA and amino acid sequence determination and analysis, sequence comparisons, subcloning in Escherichia coli strain DH5alpha, expression in Streptomyces lividans strain TK24 resulting in 42fold higher activity compared to the native wild-type Streptomyces mobaraensis strain |
| 3.5.1.14 | expressed in Escherichia coli |
| 3.5.1.14 | expressed in Escherichia coli strain BL21(DE3) |
| 3.5.1.14 | expressed in Streptomyces lividans strain TK24 |
| 3.5.1.14 | expression in Acremonium chrysogenum IS-5, transformants with pSACY2 or pMACY2 |
| 3.5.1.14 | expression in Baculovirus |
