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Literature summary for 3.5.1.14 extracted from

  • Adenan, S.; Wong, C.; Zain, H.; Azziz, S.; Rahman, R.
    Characterization of thermostable aminoacylase from Geobacillus sp. strain SZN (2019), Asia Pac. J. Mol. Biol. Biotechnol., 27, 1-9 .
No PubMed abstract available
Search for more literature for 3.5.1.14

General Stability

General Stability Organism
Zn2+, 1 mM or 10 mM, enhances thermostability Geobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information treatment of the enzyme with EDTA at 5 and 10 mM does not inhibit the activity Geobacillus sp.
PMSF 10 mM, relative activity decreases to 91.6% of control Geobacillus sp.
SDS 10 mM, relative activity decreases to 85.4% of control Geobacillus sp.
Triton X-100 5 mM and 10 mM, relative activity is decreases from 73% to 66.4% Geobacillus sp.
Tween 20 5 mM and 10 mM, relative activity is decreases from 90.7% to 85.8% Geobacillus sp.
Zn2+ 1 mM or 10 mM Geobacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1 mM, increases aminoacylase activity to 107% Geobacillus sp.
Mg2+ 1 mM, increases aminoacylase activity to 153% Geobacillus sp.
Mn2+ 1 mM, increases aminoacylase activity to 160% Geobacillus sp.
Na+ 1 mM, increases aminoacylase activity to 136% Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 SDS-PAGE Geobacillus sp.

Organism

Organism UniProt Comment Textmining
Geobacillus sp.
-
-
-
Geobacillus sp. SZN
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66.4
-
 pH 7.5, 60°C Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-methionine + H2O
-
 Geobacillus sp. acetic acid + L-methionine
-
 ?
N-acetyl-L-methionine + H2O
-
 Geobacillus sp. SZN acetic acid + L-methionine
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Geobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 30°C: about 37.6% of maximal activity, 90°C: about 27% of maximal activity Geobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermostable enzyme based on the alpha-helical structure integrity and functional stability in high temperatures Geobacillus sp.
60
-
 20 h, 53% loss of activity. 32 h, 72% loss of activity Geobacillus sp.
70
-
 the alpha-helix, where notable metal binding and catalytic residues are located, is totally weakened at temperatures above 70°C and thus results in loss of activity. The loss of the alpha-helical structure can explain drastic deterioration of activity at temperatures beyond 70°C Geobacillus sp.
80
-
 thermal denaturation point Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Geobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 11 pH 4.0: 59% of maximal activity, pH 11.0: 19% of maximal activity Geobacillus sp.