EC Number   |
|---|
    3.5.1.14 | - |
| 3.5.1.14 | ammonium sulfate precipitation, octyl-Sepharose column chromatography, HiTrap chelating column chromatography, and DEAE Sephadex A-50 ion exchange chromatography |
| 3.5.1.14 | anion exchange chromatography, hydrophobic interaction chromatography |
| 3.5.1.14 | both short acyl aminoacylase and long acyl aminoacylase |
| 3.5.1.14 | by a method that includes DEAE-Sepharose column, Chelating-Sepharose column, Amicon PM 30 membrane and Sephacryl-S200 column |
| 3.5.1.14 | commercial preparation |
| 3.5.1.14 | from frass: ion exchange chromatography (Q-Sepharose), gel filtration, ion exchange chromatography (Mono-Q, gel filtration), recombinant protein from inclusion bodies: immobilized metal ion affinity chromatography (Co2+) |
| 3.5.1.14 | in 50 mM Tris/HCl-buffer, pH 8 and disrupted by ultrasonication |
| 3.5.1.14 | native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis |
| 3.5.1.14 | native enzyme from intestinal epithelial mucosa, 1833fold to homogeneity, by acid precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration and concanavalin A afinity chromatography |
