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Literature summary for 3.5.1.14 extracted from
- Purification and characterization of enzymes involved in the degradation of chemotactic N-formyl peptides (2005), Biochemistry, 44, 8514-8522.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 6% inhibition at 10 mM | Rattus norvegicus |  |
| DTT | 85% inhibition at 20 mM | Rattus norvegicus | |
| EDTA | 10% inhibition at 50 mM | Rattus norvegicus | |
| iodoacetamide | 90% inhibition at 10 mM | Rattus norvegicus | |
| Nepsilon-formyl-Nepsilon-hydroxy-L-lysine | IC50: 1.0 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
N-acetylmethionine | pH 8.0, 22°C | Rattus norvegicus | |
| 3.1 | - |
N-formylmethionine | pH 8.0, 22°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | activates 2-3fold at 0.1 mM | Rattus norvegicus | |
| NiCl2 | activates 2-3fold at 0.1 mM | Rattus norvegicus | |
| Zn2+ | 0.98 Zn2+ per enzyme molecule, tightly bound, involved in cataylsis | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | the enzyme is involved in the degradation of chemotactic N-formyl peptides and plays a protective role in degrading bacterial and mitochondrial N-formylated peptide together with the alpha-N-acylpeptide hydrolase, EC 3.4.19.1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from intestinal epithelial mucosa, 1833fold to homogeneity, by acid precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration and concanavalin A afinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mucosa | intestinal | Rattus norvegicus | - |
| small intestine | mucosa | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 2.2 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is involved in the degradation of chemotactic N-formyl peptides and plays a protective role in degrading bacterial and mitochondrial N-formylated peptide together with the alpha-N-acylpeptide hydrolase, EC 3.4.19.1 | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with N-formylmethionylalanine, N-formylalanylalanine, N-formylmethionylalanylserine, N-acetylmethionylalanylserine, and N-formylmethionylleucylphenylalanine | Rattus norvegicus | ? | - |
? | |
| N-acetylmethionine + H2O | preferred substrate | Rattus norvegicus | acetate + L-methionine | - |
? | |
| N-formylmethionine + H2O | substrate synthesis and purification, overview | Rattus norvegicus | formate + L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fMDF | - |
Rattus norvegicus |
| N-acylase IA | - |
Rattus norvegicus |
| N-formylmethionine deformylase | - |
Rattus norvegicus |
| Nalpha-acyl-L-amino acid amidohydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
N-acetylmethionine | pH 8.0, 22°C | Rattus norvegicus | |
| 7.9 | - |
N-formylmethionine | pH 8.0, 22°C | Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rattus norvegicus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 1 | - |
IC50: 1.0 mM | Rattus norvegicus | Nepsilon-formyl-Nepsilon-hydroxy-L-lysine | |
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