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Literature summary for 3.5.1.14 extracted from
- A lepidopteran aminoacylase (L-ACY-1) in Heliothis virescens (Lepidoptera: Noctuidae) gut lumen hydrolyzes fatty acid-amino acid conjugates, elicitors of plant defense (2012), Insect Biochem. Mol. Biol., 42, 32-40.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Heliothis virescens |  |
| Phenanthroline | - |
Heliothis virescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | restores activity after treatment with EDTA or phenanthroline | Heliothis virescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
x * 64000, recombinant protein about 50000, SDS-PAGE | Heliothis virescens |
| 197000 | - |
gel filtration | Heliothis virescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heliothis virescens | G8EJ32 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from frass: ion exchange chromatography (Q-Sepharose), gel filtration, ion exchange chromatography (Mono-Q, gel filtration), recombinant protein from inclusion bodies: immobilized metal ion affinity chromatography (Co2+) | Heliothis virescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | gut lumen: fatty acid-amino acid conjugates hydrolase from frass collected from fifth instar larvae | Heliothis virescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000028 | - |
substrate N-linolenoyl-L-glutamine, protein partially purified from frass, pH 8.0, 20°C | Heliothis virescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-linolenoyl-L-glutamine + H2O | - |
Heliothis virescens | linolenic acid + L-glutamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, recombinant protein about 50000, SDS-PAGE | Heliothis virescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminoacylase | - |
Heliothis virescens |
| lepidopteran aminoacylase 1 | - |
Heliothis virescens |
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Release 2023.1 (January 2023)
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