Information on EC 3.4.16.5 - carboxypeptidase C

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
3.4.16.5
-
RECOMMENDED NAME
GeneOntology No.
carboxypeptidase C
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of a C-terminal amino acid with broad specificity
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
9046-67-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CDP-AI
-
-
Manually annotated by BRENDA team
CDP-AI
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Citrus sp.
; orange
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
IFO 4478
-
-
Manually annotated by BRENDA team
cultivars Malviya-36 and Pant-12
-
-
Manually annotated by BRENDA team
strain CBS 5905
-
-
Manually annotated by BRENDA team
strain CBS 5905
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain BJ2168
-
-
Manually annotated by BRENDA team
strain BY4741
-
-
Manually annotated by BRENDA team
strain EOS1
-
-
Manually annotated by BRENDA team
strain Y
SwissProt
Manually annotated by BRENDA team
strain Y
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
mice with a double CathA/Scpep1 deficiency (DD mice) demonstrate hypertension, increased ET-1-induced vasoconstriction and prolonged half-life of circulating ET-1 as compared to both wild-type animals and those with single CathA or Scpep1 deficiencies
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-dimethylaminonaphthalene-1-sulfonyl-Phe-Leu-Arg + H2O
5-dimethylaminonaphthalene-1-sulfonyl-Phe-Leu + Arg
show the reaction diagram
-
-
-
?
9-(R)-4'-(R)-[[[(S)-1-[(ethoxycarbonyl)ethyl]amino]phenoxyphosphinyl]methoxy]-2'-fluoro-1'-furanyladenine + H2O
?
show the reaction diagram
-
-
-
-
?
9-fluorenylmethoxycarbonyl-Glu-Ala + H2O
9-fluorenylmethoxycarbonyl-Glu + Ala
show the reaction diagram
-
-
-
-
?
9-fluorenylmethoxycarbonyl-Glu-Glu-Ala + H2O
9-fluorenylmethoxycarbonyl-Glu-Glu + Ala
show the reaction diagram
-
-
-
-
?
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Ala + H2O
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu + Ala
show the reaction diagram
-
-
-
-
?
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Ala + H2O
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu + Ala
show the reaction diagram
-
-
-
-
?
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu-Ala + H2O
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu + Ala
show the reaction diagram
-
-
-
-
?
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu-Glu-Ala + H2O
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu-Glu + Ala
show the reaction diagram
-
-
-
-
?
9-[(R)-2-[[(S)-[[(S)-1-(isopropoxycarbonyl)ethyl]amino]phenoxyphosphinyl]methoxy]-propyl]adenine + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-Phe ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-Phe-Leu + H2O
acetyl-Phe + Leu
show the reaction diagram
-
-
-
-
?
acetyl-Phe-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
Acetyl-Tyr ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-Tyr-ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
adrenocorticotropic hormone fragment 7-38 + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Ala benzyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Ala-OGly + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Ala-OMe + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-4-nitroanilide + H2O
benzoyl-Arg + 4-nitrolaniline
show the reaction diagram
benzoyl-Gly benzyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-Arg + H2O
benzoyl-Gly + Arg
show the reaction diagram
benzoyl-Gly-beta-phenyllactate + H2O
?
show the reaction diagram
benzoyl-Gly-Lys + H2O
benzoyl-Gly + Lys
show the reaction diagram
-
isoenzyme MpiCP-2
-
-
?
benzoyl-Gly-OEt + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-OGly + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-OMe + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-OPhe + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-Phe + H2O
benzoyl-Gly + Phe
show the reaction diagram
-
-
-
-
?
benzoyl-L-Tyr-4-nitroanilide + H2O
benzoyl-Tyr + 4-nitroaniline
show the reaction diagram
benzoyl-L-tyrosine-p-nitroanilide + H2O
benzoyl-L-tyrosine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-Phe-Gly + H2O
benzoyl-Phe + Gly
show the reaction diagram
-
-
-
-
?
benzoyl-Phe-OGly + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Phe-OMe + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Tyr-4-nitroanilide + H2O
benzoyl-Tyr + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala + H2O
benzyloxycarbonyl-Ala + Ala
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Glu + H2O
benzyloxycarbonyl-Ala + Glu
show the reaction diagram
Benzyloxycarbonyl-Ala-Leu + H2O
Benzyloxycarbonyl-Ala + Leu
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Ala-Phe + H2O
Benzyloxycarbonyl-Ala + Phe
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-Pro + H2O
benzyloxycarbonyl-Arg + Pro
show the reaction diagram
Citrus sp.
-
-
-
-
?
benzyloxycarbonyl-Glu-Leu + H2O
benzyloxycarbonyl-Glu + Leu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Glu-Phe + H2O
benzyloxycarbonyl-Glu + Phe
show the reaction diagram
Benzyloxycarbonyl-Glu-Tyr + H2O
Benzyloxycarbonyl-Glu + Tyr
show the reaction diagram
benzyloxycarbonyl-Gly-Arg + H2O
benzyloxycarbonyl-Gly + Arg
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Glu + H2O
benzyloxycarbonyl-Gly + Glu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Gly + H2O
benzyloxycarbonyl-Gly + Gly
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Gly-Phe + H2O
benzyloxycarbonyl-Gly-Gly + Phe
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Gly-Leu + H2O
Benzyloxycarbonyl-Gly + Leu
show the reaction diagram
benzyloxycarbonyl-Gly-Met + H2O
benzyloxycarbonyl-Gly + Met
show the reaction diagram
-
19% activity compared to benzyloxycarbonyl-Glu-Tyr
-
-
?
Benzyloxycarbonyl-Gly-Phe + H2O
Benzyloxycarbonyl-Gly + Phe
show the reaction diagram
benzyloxycarbonyl-Gly-Pro + H2O
benzyloxycarbonyl-Gly + Pro
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O
benzyloxycarbonyl-Gly-Pro-Leu + Gly
show the reaction diagram
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O
benzyloxycarbonyl-Gly-Pro-Leu-Gly + Pro
show the reaction diagram
-
3% activity compared to benzyloxycarbonyl-Glu-Tyr
-
-
?
Benzyloxycarbonyl-Gly-Val + H2O
Benzyloxycarbonyl-Gly + Val
show the reaction diagram
-
24% activity compared to benzyloxycarbonyl-Glu-Tyr
-
-
?
benzyloxycarbonyl-His-Leu + H2O
benzyloxycarbonyl-His + Leu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-His-Phe + H2O
benzyloxycarbonyl-His + Phe
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-His-Tyr + H2O
benzyloxycarbonyl-His + Tyr
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ile-Leu + H2O
benzyloxycarbonyl-Ile + Leu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Phe-L-Leu + H2O
benzyloxycarbonyl-L-Phe + L-Leu
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Leu-Leu + H2O
benzyloxycarbonyl-Leu + Leu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Leu-Phe + H2O
benzyloxycarbonyl-Leu + Phe
show the reaction diagram
benzyloxycarbonyl-Nle-Leu + H2O
benzyloxycarbonyl-Nle + Leu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Ala + H2O
benzyloxycarbonyl-Phe + Ala
show the reaction diagram
benzyloxycarbonyl-Phe-beta-Ala + H2O
benzyloxycarbonyl-Phe + beta-Ala
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Glu + H2O
benzyloxycarbonyl-Phe + Glu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Gly + H2O
benzyloxycarbonyl-Phe + Gly
show the reaction diagram
benzyloxycarbonyl-Phe-His + H2O
benzyloxycarbonyl-Phe + His
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Leu + H2O
benzyloxycarbonyl-Phe + Leu
show the reaction diagram
benzyloxycarbonyl-Phe-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Phe + H2O
benzyloxycarbonyl-Phe + Phe
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Pro + H2O
benzyloxycarbonyl-Phe + Pro
show the reaction diagram
Benzyloxycarbonyl-Phe-Tyr + H2O
Benzyloxycarbonyl-Phe + Tyr
show the reaction diagram
benzyloxycarbonyl-Phe-Tyr-Gly + H2O
benzyloxycarbonyl-Phe-Tyr + Gly
show the reaction diagram
-
172% activity compared to benzyloxycarbonyl-Glu-Tyr
-
-
?
benzyloxycarbonyl-Phe-Tyr-Leu + H2O
benzyloxycarbonyl-Phe-Tyr + Leu
show the reaction diagram
-
120% activity compared to benzyloxycarbonyl-Glu-Tyr
-
-
?
benzyloxycarbonyl-Pro-Leu + H2O
benzyloxycarbonyl-Pro + Leu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Pro-Leu-Gly + H2O
benzyloxycarbonyl-Pro-Leu + Gly
show the reaction diagram
-
isoenzyme MpiCP-2
-
-
?
benzyloxycarbonyl-Ser-Leu + H2O
benzyloxycarbonyl-Ser + Leu
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Tyr-Glu + H2O
benzyloxycarbonyl-Tyr + Glu
show the reaction diagram
benzyloxycarbonyl-Tyr-Gly + H2O
benzyloxycarbonyl-Tyr + Gly
show the reaction diagram
-
188% activity compared to benzyloxycarbonyl-Glu-Tyr
-
-
?
benzyloxycarbonyl-Tyr-Phe + H2O
benzyloxycarbonyl-Tyr + Phe
show the reaction diagram
-
201% activity compared to benzyloxycarbonyl-Glu-Tyr
-
-
?
benzyloxycarbonyl-Val-Leu + H2O
benzyloxycarbonyl-Val + Leu
show the reaction diagram
-
-
-
-
?
bombesin + H2O
?
show the reaction diagram
-
-
-
-
?
carboxypropionyl-Phe-4-nitroanilide + H2O
carboxypropionyl-Phe + 4-nitroaniline
show the reaction diagram
CBZ-Phe-Leu + H2O
N-benzyloxycarbonyl-L-Phe + L-leucine
show the reaction diagram
-
-
-
-
?
corn gluten + H2O
?
show the reaction diagram
-
-
-
-
?
corticotropin-releasing factor fragment 6-33 + H2O
?
show the reaction diagram
-
-
-
-
?
cyclo-statherin Q-37 + H2O
?
show the reaction diagram
-
-
-
-
?
endothelin I + H2O
endothelin(1-20) + Trp
show the reaction diagram
furylacryloyl-Ala-Arg + H2O
furylacryloyl-Ala + Arg
show the reaction diagram
furylacryloyl-Ala-Glu + H2O
furylacryloyl-Ala + Glu
show the reaction diagram
-
-
-
-
?
furylacryloyl-Ala-Leu + H2O
furylacryloyl-Ala + Leu
show the reaction diagram
-
-
-
-
?
furylacryloyl-Ala-Lys + H2O
furylacryloyl-Ala + Lys
show the reaction diagram
furylacryloyl-Arg-Leu + H2O
furylacryloyl-Arg + Leu
show the reaction diagram
-
preferred substrate
-
-
?
furylacryloyl-Lys-Ala + H2O
furylacryloyl-Lys + Ala
show the reaction diagram
-
-
-
-
?
furylacryloyl-Lys-Leu + H2O
furylacryloyl-Lys + leu
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe ethyl ester + H2O
?
show the reaction diagram
furylacryloyl-Phe-Ala + H2O
furylacryloyl-Phe + Ala
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-Ala + H2O
furylacryloyl-Phe + Phe
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-Gly + H2O
?
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-Leu + H2O
?
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-Leu + H2O
furylacryloyl-Phe + Leu
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-NHEt + H2O
?
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-OGly + H2O
?
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-OMe + H2O
?
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-Phe + H2O
furylacryloyl-Phe + Phe
show the reaction diagram
-
-
-
-
?
furylacryloyl-Phe-Val + H2O
furylacryloyl-Phe + Val
show the reaction diagram
-
-
-
-
?
FVNQHLCGSHLV + H2O
FVNQHLCGSH + L-Leu-L-Val + L-Val
show the reaction diagram
-
-
-
-
?
FVNQHLCGSHLVEAL + H2O
FVNQHLCGSHLVE + L-Ala-L-Leu + L-Leu
show the reaction diagram
-
-
-
-
?
Glu-Asp-Glu-Phe-Phe-Leu-Ala + H2O
Glu-Asp-Glu-Phe-Phe-Leu + Ala
show the reaction diagram
-
-
-
-
?
Glucagon + H2O
?
show the reaction diagram
-
-
-
-
?
hippuryl-beta-phenyl lactate + H2O
?
show the reaction diagram
-
-
-
-
?
isoaspartyl peptides + H2O
isoaspartyl dipeptides
show the reaction diagram
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
show the reaction diagram
lysosomal neuraminidase-1 + H2O
?
show the reaction diagram
-
lysosomal neuraminidase-1 gains full catalytic activity in the lysosome through its binding to PPCA
-
-
?
N-((S)-5-amino-5-(carbamoyl)pentyl)-3-(aminooxymethyl)benzamide + H2O
?
show the reaction diagram
-
-
-
-
?
N-(2-furanacryloyl)-Phe-Phe + H2O
N-(2-furanacryloyl)-Phe + Phe
show the reaction diagram
-
-
-
-
?
N-(4-methoxyphenyl-azoformyl)-L-Phe-OH + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-renin tetradecapeptide + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-tyrosine-p-nitroanilide + H2O
N-benzoyl-L-Tyr + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-tyrosine-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
show the reaction diagram
N-carbobenzoxy-L-Phe-L-Ala + H2O
N-carbobenzoxy-L-Phe + L-Ala
show the reaction diagram
-
-
-
-
?
ochratoxin A + H2O
ochratoxin alpha
show the reaction diagram
oxidized insulin B + H2O
?
show the reaction diagram
-
-
-
-
?
pancreatic carboxypeptidase A + H2O
hydrolyzed carboxypeptidase A + C-terminal amino acid
show the reaction diagram
-
-
-
?
pancreatic carboxypeptidase B + H2O
hydrolyzed pancreatic carboxypeptidase B + C-terminal amino acid
show the reaction diagram
-
-
-
?
pepsin-digested soy protein + H2O
?
show the reaction diagram
-
-
-
-
?
phytochelatin + H2O
phytochelatin 2 + ?
show the reaction diagram
poly-alpha-L-aspartic acid + H2O
?
show the reaction diagram
-
slowly
-
-
-
poly-alpha-L-glutamic acid + H2O
glutamic acid
show the reaction diagram
-
-
-
?
succinyl-L-Ile-L-Ile-L-Trp-7-amido-4-methylcoumarin + H2O
succinyl-L-Ile-L-Ile-L-Trp + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Tamm-Horsfall glycoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
trypsin-digested casein + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactor requirement
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
As3+
-
marked increase of activity at 0.05 mM
CaCl2
-
1 mM, 18% inhibition, isoenzyme MpiCP-1
Cd2+
-
stimulates activity
CdCl2
-
1 mM, 18% inhibition, isoenzyme MpiCP-2
CoCl2
-
1 mM, 23% inhibition, isoenzyme MpiCP-1
CuCl
-
1 mM, 33% inhibition, isoenzyme MpiCP-1; 1 mM, 84% inhibition, isoenzyme MpiCP-2
CuCl2
-
1 mM, 36% inhibition, isoenzyme MpiCP-1; 1 mM, 73% inhibition, isoenzyme MpiCP-2
FeCl2
-
1 mM, 35% inhibition, isoenzyme MpiCP-1; 1 mM, 73% inhibition, isoenzyme MpiCP-2
FeCl3
-
1 mM, 37% inhibition, isoenzyme MpiCP-1; 1 mM, 86% inhibition, isoenzyme MpiCP-2
HgCl2
-
1 mM, complete inhibition, isoenzyme MpiCP-2
MgCl2
-
1 mM, 15% inhibition, isoenzyme MpiCP-1
NiCl2
-
1 mM, 12% inhibition, isoenzyme MpiCP-2
PbCl2
-
1 mM, 47% inhibition, isoenzyme MpiCP-2
SnCl2
-
1 mM, 47% inhibition, isoenzyme MpiCP-2
Zinc
Citrus sp.
-
possibly contains zinc in the active site, orange peel
ZnSO4
-
1 mM, 46% inhibition, isoenzyme MpiCP-2
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3R)-3-([(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(2-chlorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methoxyphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(3-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(4-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-[2-(trifluoromethyl)phenyl]propanoic acid
-
-
-
(3S)-3-(([1-(3-chlorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(3-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([1-(4-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([4-chloro-1-(2-fluorophenyl)-5-methoxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(([4-fluoro-1-(2-fluorophenyl)-5-methoxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-(2,4-dichlorophenyl)-3-([[5-(3,3-dimethyl-2-oxobutoxy)-1-(2-fluorophenyl)-1H-pyrazol-3-yl]carbonyl]amino)propanoic acid
-
-
(3S)-3-(2,4-dichlorophenyl)-3-[([1-(2-fluorophenyl)-5-[(2R)-2-hydroxy-3,3-dimethylbutoxy]-1H-pyrazol-3-yl]carbonyl)amino]propanoic acid
-
-
(3S)-3-(2,4-dichlorophenyl)-3-[([1-(2-fluorophenyl)-5-[(2S)-2-hydroxy-3,3-dimethylbutoxy]-1H-pyrazol-3-yl]carbonyl)amino]propanoic acid
-
-
(3S)-3-([(4-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-([(4-methoxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-([(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
-
-
-
(3S)-3-([[5-(3,3-dimethyl-2-oxobutoxy)-1-(2-fluorophenyl)-1H-pyrazol-3-yl]carbonyl]amino)-3-(2-methylphenyl)propanoic acid
-
-
(3S)-3-([[5-(cyclopropylmethoxy)-1-(2-fluorophenyl)-1H-pyrazol-3-yl]carbonyl]amino)-3-(2-methylphenyl)propanoic acid
-
-
(3S)-3-([[5-ethoxy-1-(2-fluorophenyl)-1H-pyrazol-3-yl]carbonyl]amino)-3-(2-methylphenyl)propanoic acid
-
-
(3S)-3-[([1-(2-fluorophenyl)-5-[(2S)-2-hydroxy-3,3-dimethylbutoxy]-1H-pyrazol-3-yl]carbonyl)amino]-3-(2-methylphenyl)propanoic acid
-
-
(S)-1-chloro-4-phenyl-3-tosylamido-2-butanone
-
37% residual activity at 1 mM
1,10-phenanthroline
-
-
1,2-epoxy-3-(p-nitrophenoxy)propane
-
1 mM, 14% inhibition, isoenzyme MpiCP-2
2,2'-dipyridyl
-
1 mM, 21% inhibition, isoenzyme MpiCP-1; 1 mM, 22% inhibition, isoenzyme MpiCP-2
2-mercaptoethanol
2-propanol
Citrus sp.
-
above 5%
3,4-dichloroisocoumarin
-
effective inhibitor
3-(([1-(2,5-dimethylphenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(4-fluorophenyl)propanoic acid
-
-
-
3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-phenylpropanoic acid
-
-
-
3-(([1-(2-fluorophenyl)-5-methoxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
-
-
-
3-(([1-(4-chlorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2,5-dichlorophenyl)propanoic acid
-
-
-
3-(2,3-dichlorophenyl)-3-(([1-(2,4-difluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)propanoic acid
-
-
-
3-(2,3-dichlorophenyl)-3-([(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)propanoic acid
-
-
-
3-(4'-fluorobiphenyl-4-yl)-3-([[1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl]amino)propanoic acid
-
-
3-(5-fluoro-2-methylphenyl)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)propanoic acid
-
-
-
3-(biphenyl-4-yl)-3-([[1-(2,5-dimethylphenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl]amino)propanoic acid
-
-
3-Phenyl-1-propanol
-
inhibits hydrolysis of benzyloxycarbonyl-Phe-Leu, activates hydrolysis of benzyloxycarbonyl-Gly-Phe
3-[[(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino]-3-(4-phenoxyphenyl)propanoic acid
-
-
4-Aminobenzylsuccinic acid
-
-
acetyl-D-Phe
-
-
acetyl-D-Phe ethyl ester
-
-
acetyl-D-Phe-ethyl ester
-
-
benzyloxycarbonyl-D-Phe
-
-
benzyloxycarbonyl-D-Phe-D-Leu
-
-
benzyloxycarbonyl-Gly-Leu-Phe-CH2Cl
-
-
Benzyloxycarbonyl-L-phenylalanine chloromethyl ketone
Benzyloxycarbonyl-L-phenylalanyl chloromethane
-
diisopropylphosphorofluoridate-inactivated enzyme does not react with the inhibitor
Benzyloxycarbonyl-Phe
-
-
beta-phenyl-L-lactate
-
-
Beta-phenylpropionate
-
-
carboxypeptidase inhibitor IC
-
-
-
carboxypeptidase Y inhibitor from baker's yeast
-
MW 23400-24000, purification, chemical and physical properties
-
carboxypeptidase Y inhibitor IC
-
-
-
chymostatin
CoSO4
-
62% residual activity at 10 mM
CuCl
-
1 mM, 37% inhibition
CuCl2
-
1 mM, 39% inhibition
CuSO4
-
4% residual activity at 10 mM
D-Amino acids
-
less inhibitory than the L-enantiomers, non-competitive or mixed-type
D-Phe ethyl ester
-
-
diisopropyl fluorophosphate
diisopropylfluorophosphate
dimethylformamide
-
-
E-64
-
1 mM, 10% inhibition, isoenzyme MpiCP-2; 1 mM, 22% inhibition, isoenzyme MpiCP-1
ebelactone B
Elastatinal
-
1 mM, 46% inhibition, isoenzyme MpiCP-2
ethanol
FeCl3
-
1 mM, 92% inhibition
iodoacetamide
-
slight
L-amino acids
-
competitive
lactacystin
Leupeptin
-
1 mM, 24% inhibition, isoenzyme MpiCP-1; 1 mM, 60% inhibition, isoenzyme MpiCP-2
MnCl2
-
1 mM, 14% inhibition
Monoiodoacetic acid
N-(N-[1-trans-3-carboxyoxiran-2-carbonyl]-L-leucyl)-agmatine
-
93% residual activity at 1 mM
Nalpha-tosyl-L-Lys-chloromethyl ketone
Nalpha-tosyl-L-Phe-chloromethyl ketone
NEM
-
1 mM, 71% inhibition
NH2-blocked amino acids
-
-
-
omuralide
p-chloromercuribenzenesulfonate
-
-
p-chloromercuribenzoic acid
-
complete inhibition at 1 mM, at 30C and pH 3.1
p-hydroxymercuribenzene sulfonate
-
mercurials inhibit the hydrolysis of the good substrate benzyloxycarbonyl-L-Phe-L-Leu, the inhibition is repressed by the competitive inhibitors benzyloxycarbonyl-D-Phe-D-Leu-Leu-Phe, trans-cinnamate and acetyl-D-Phe ethyl ester. Aromatic, methyl and ethyl mercurials do not cause complete inactivation with the poor substrates benzyloxycarbonyl-Gly-Phe and benzoyl-Gly-beta,L-phenyllactate. Propyl and butyl-mercurials enhance these activities
p-hydroxymercuribenzoate
Pb(CH3COO)2
-
60% residual activity at 10 mM
PbCl2
-
1 mM, 95% inhibition
pepstatin A
Phe-Asn-Arg-Ala-Val-Asp
-
-
Phe-Asn-Arg-Ala-Val-Val
-
-
Phe-Asn-Arg-Pro-Val-Asp
-
-
Phe-Asn-Arg-Pro-Val-Val
-
-
Phe-ethyl ester
-
-
phenylmethane sulfonylfluoride
-
-
phenylmethylsulfonyl fluoride
-
irreversible
Phenylmethylsulfonylfluoride
phosphoramidon
-
1 mM, 17% inhibition, isoenzyme MpiCP-1; 1 mM, 41% inhibition, isoenzyme MpiCP-2
piperastatin A
poly-L-lysine
-
-
poststatin
Product analogs
-
-
-
SDS
-
1 mM, complete inhibition
SnCl2
-
1 mM, complete inhibition
Substrate analogs
-
-
-
Tfs1p
-
NatB-dependent acetylation is essential for the inhibitory activity on carboxypeptidase Y
-
tosylphenylalanylchloromethane
-
1 mM, 35% inhibition, isoenzyme MpiCP-1
trans-epoxysuccinyl-L-leucylamido-(4-guanidine)-butane
-
-
ZnSO4
-
69% residual activity at 10 mM
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-chloro-3-tosylamido-2-heptanone
-
10% increase of activity at 10 mM
3-Phenyl-1-propanol
-
inhibits hydrolysis of benzyloxycarbonyl-Phe-Leu, activates hydrolysis of benzyloxycarbonyl-Gly-Phe
antipain
-
15 increase of activity at 0.1 mM
CH3-CH2-CH2-CH2-Hg
-
mercurials inhibit the hydrolysis of the good substrate benzyloxycarbonyl-L-Phe-L-Leu, the inhibition is repressed by the competitive inhibitors benzyloxycarbonyl-D-Phe-D-Leu-Leu-Phe, trans-cinnamate and acetyl-D-Phe ethyl ester. Aromatic, methyl and ethyl mercurials do not cause complete inactivation with the poor substrates benzyloxycarbonyl-Gly-Phe and benzoyl-Gly-beta,L-phenyllactate. Propyl and butyl-mercurials enhance these activities
CH3-CH2-CH2-Hg
-
mercurials inhibit the hydrolysis of the good substrate benzyloxycarbonyl-L-Phe-L-Leu, the inhibition is repressed by the competitive inhibitors benzyloxycarbonyl-D-Phe-D-Leu-Leu-Phe, trans-cinnamate and acetyl-D-Phe ethyl ester. Aromatic, methyl and ethyl mercurials do not cause complete inactivation with the poor substrates benzyloxycarbonyl-Gly-Phe and benzoyl-Gly-beta,L-phenyllactate. Propyl and butyl-mercurials enhance these activities
EDTA
-
25% increase of activity at 10 mM
Kar2p, Pdi1p, Ero1p
-
co-expression of Kar2p, Pdi1p and Ero1p give a synergistic effect on CPY expression, of which activity is 1.7times higher than that of the control strain
-
Karp2
-
a single co-expression of Kar2p leads to a 28% enhancement in extracellular CPY activity, relative to the control strain
-
Leupeptin
-
20% increase of activity at 0.1 mM
N-ethylmaleimide
-
8% increase of activity at 10 mM
o-phenanthroline
-
4% increase of activity at 5 mM
pepstatin A
-
5% increase of activity at 0.1 mM
proteinase A
-
active proteinase A is essential to the activity of carboxypeptidase Y
-
proteinase K
-
converts the proenzyme to the mature, active enzyme
-
additional information
-
FUSCA, a B3 domain transcription factor that lacks the N-terminal activation and B1 domain 3 activates the phaseolin promoter in the presence of abscisic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.363
9-(R)-4'-(R)-[[[(S)-1-[(ethoxycarbonyl)ethyl]amino]phenoxyphosphinyl]methoxy]-2'-fluoro-1'-furanyladenine
-
-
0.0082 - 0.21
9-fluorenylmethoxycarbonyl-Glu-Glu-Ala
0.0024 - 0.009
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Ala
0.00059 - 0.0025
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Ala
0.0001 - 0.00013
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu-Ala
0.00012
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu-Glu-Ala
-
pH 6.5
0.61
9-[(R)-2-[[(S)-[[(S)-1-(isopropoxycarbonyl)ethyl]amino]phenoxyphosphinyl]methoxy]-propyl]adenine
-
-
1.2 - 1.28
acetyl-Phe ethyl ester
0.74
Acetyl-Phe-Leu
-
pH 6.5, 25C
2.4
Acetyl-Tyr-ethyl ester
0.07
benzoyl-Ala benzyl ester
-
pH 7.5
14
benzoyl-Ala-OGly
-
pH 6.5
6.3
benzoyl-Ala-OMe
-
pH 7.5
1.7
benzoyl-Gly benzyl ester
-
pH 7.5
10
benzoyl-Gly-Arg
-
pH 6.5, 25C
0.45
benzoyl-Gly-beta-phenyllactate
-
pH 6.0, 25C
21
benzoyl-Gly-OEt
-
pH 7.5
56
benzoyl-Gly-OGly
-
pH 6.5
48
benzoyl-Gly-OMe
-
pH 7.5
0.05
benzoyl-Gly-OPhe
-
pH 6.5
7.7
benzoyl-Gly-Phe
-
pH 6.5, 25C
1.1
benzoyl-Phe-Gly
-
pH 6.5
2.4
benzoyl-Phe-OGly
-
pH 6.5
0.18
benzoyl-Phe-OMe
-
pH 7.5
0.13
benzoyl-Tyr-p-nitroanilide
-
-
3.26
Benzyloxycarbonyl-Ala-Ala
-
pH 6.5, 25C
0.57 - 4.2
Benzyloxycarbonyl-Ala-Phe
4
benzyloxycarbonyl-Arg-Pro
Citrus sp.
-
orange peel
0.053 - 3.3
benzyloxycarbonyl-Glu-Phe
0.14 - 3
Benzyloxycarbonyl-Glu-Tyr
1.15 - 9.09
Benzyloxycarbonyl-Gly-Glu
1.75
benzyloxycarbonyl-Gly-Gly
-
pH 6.5, 25C
0.83 - 9.1
Benzyloxycarbonyl-Gly-Leu
0.41 - 19
Benzyloxycarbonyl-Gly-Phe
0.1 - 0.38
Benzyloxycarbonyl-His-Phe
0.83 - 1.82
benzyloxycarbonyl-His-Tyr
0.068
benzyloxycarbonyl-Leu-Leu
-
pH 6.5, 25C
0.1 - 0.5
benzyloxycarbonyl-Leu-Phe
0.56
benzyloxycarbonyl-Phe-Ala
-
pH 6.5, 25C
9
benzyloxycarbonyl-Phe-beta-Ala
-
pH 6.5, 25C
-
0.36 - 16.5
benzyloxycarbonyl-Phe-Glu
4
benzyloxycarbonyl-Phe-Gly
0.9 - 3.2
benzyloxycarbonyl-Phe-His
0.07 - 3
Benzyloxycarbonyl-Phe-Leu
10 - 15
benzyloxycarbonyl-Phe-NH2
0.67
benzyloxycarbonyl-Phe-Pro
-
pH 6.5, 25C
-
0.86 - 1.55
benzyloxycarbonyl-Tyr-Glu
0.04
CBZ-Phe-Leu
-
recombinant protein, pH 4.5, 27C
-
0.022
endothelin
-
-
-
0.075
Furylacryloyl-Phe ethyl ester
-
wild-type
0.14
furylacryloyl-Phe-Ala
-
pH 6.5
5.4
furylacryloyl-Phe-Gly
-
pH 6.5
0.021
furylacryloyl-Phe-Leu
-
pH 6.5
0.16
Furylacryloyl-Phe-OGly-OH
-
wild-type
0.39
furylacryloyl-Phe-OMe
-
pH 7.5
0.047
furylacryloyl-Phe-Val
-
pH 6.5
0.45
Hippuryl-beta-phenyl lactate
-
-
0.029
N-benzoyl-L-tyrosine-p-nitroanilide
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
237
9-(R)-4'-(R)-[[[(S)-1-[(ethoxycarbonyl)ethyl]amino]phenoxyphosphinyl]methoxy]-2'-fluoro-1'-furanyladenine
Homo sapiens
-
-
2.7 - 6.7
9-fluorenylmethoxycarbonyl-Glu-Glu-Ala
2.2 - 3.8
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Ala
2.5 - 3.9
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Ala
2.3 - 3.7
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu-Ala
3.5
9-fluorenylmethoxycarbonyl-Glu-Glu-Glu-Glu-Glu-Glu-Ala
Saccharomyces cerevisiae
-
pH 5.0
1777
9-[(R)-2-[[(S)-[[(S)-1-(isopropoxycarbonyl)ethyl]amino]phenoxyphosphinyl]methoxy]-propyl]adenine
Homo sapiens
-
-
120 - 122
acetyl-Phe ethyl ester
36.1
Acetyl-Phe-Leu
Saccharomyces cerevisiae
-
pH 6.5, 25C
105
Acetyl-Tyr ethyl ester
123
benzoyl-Ala benzyl ester
Saccharomyces cerevisiae
-
pH 7.5
58.3
benzoyl-Ala-OGly
Saccharomyces cerevisiae
-
pH 6.5
51.7
benzoyl-Ala-OMe
Saccharomyces cerevisiae
-
pH 7.5
18.3
benzoyl-Gly benzyl ester
Saccharomyces cerevisiae
-
pH 7.5
1.44
benzoyl-Gly-Arg
Saccharomyces cerevisiae
-
pH 6.5, 25C
21.2
benzoyl-Gly-beta-phenyllactate
Saccharomyces cerevisiae
-
pH 6.0, 25C
2.5
benzoyl-Gly-OEt
Saccharomyces cerevisiae
-
pH 7.5
30
benzoyl-Gly-OGly
Saccharomyces cerevisiae
-
pH 6.5
2.5
benzoyl-Gly-OMe
Saccharomyces cerevisiae
-
pH 7.5
18.3
benzoyl-Gly-OPhe
Saccharomyces cerevisiae
-
pH 6.5
3.8 - 3.83
benzoyl-Gly-Phe
11.5
benzoyl-Phe-Gly
Saccharomyces cerevisiae
-
pH 6.5
35
benzoyl-Phe-OGly
Saccharomyces cerevisiae
-
pH 6.5
152
benzoyl-Phe-OMe
Saccharomyces cerevisiae
-
pH 7.5
2.1
benzoyl-Tyr-4-nitroanilide
Saccharomyces cerevisiae
-
-
69.4
Benzyloxycarbonyl-Ala-Ala
Saccharomyces cerevisiae
-
pH 6.5, 25C
465
Benzyloxycarbonyl-Ala-Leu
Saccharomyces cerevisiae
-
-
2.2 - 150
Benzyloxycarbonyl-Ala-Phe
13.3 - 16.8
benzyloxycarbonyl-Glu-Phe
18.8 - 23.8
Benzyloxycarbonyl-Glu-Tyr
0.32 - 0.46
Benzyloxycarbonyl-Gly-Glu
2.18
benzyloxycarbonyl-Gly-Gly
Saccharomyces cerevisiae
-
pH 6.5, 25C
0.467 - 7
Benzyloxycarbonyl-Gly-Leu
0.16 - 5.5
Benzyloxycarbonyl-Gly-Phe
0.72 - 2.05
Benzyloxycarbonyl-His-Phe
8.2 - 10.7
benzyloxycarbonyl-His-Tyr
6.85
benzyloxycarbonyl-Leu-Leu
Saccharomyces cerevisiae
-
pH 6.5, 25C
46
benzyloxycarbonyl-Leu-Phe
Saccharomyces cerevisiae
-
pH 6.5, 25C
120
benzyloxycarbonyl-Phe-Ala
Saccharomyces cerevisiae
-
pH 6.5, 25C
66
benzyloxycarbonyl-Phe-beta-Ala
Saccharomyces cerevisiae
-
pH 6.5, 25C
-
24.5 - 96.5
benzyloxycarbonyl-Phe-Glu
140
benzyloxycarbonyl-Phe-Gly
3 - 11.6
benzyloxycarbonyl-Phe-His
1.2 - 130
Benzyloxycarbonyl-Phe-Leu
2.2 - 5.33
benzyloxycarbonyl-Phe-NH2
420
benzyloxycarbonyl-Phe-Phe
23
benzyloxycarbonyl-Phe-Pro
Saccharomyces cerevisiae
-
pH 6.5, 25C
-
291 - 2039
benzyloxycarbonyl-Tyr-Glu
12.1
CBZ-Phe-Leu
Homo sapiens
-
recombinant protein, pH 4.5, 27C
-
24.2
endothelin
Saccharomyces cerevisiae
-
-
-
68.3
Furylacryloyl-Phe ethyl ester
Saccharomyces cerevisiae
-
wild-type enzyme
162
furylacryloyl-Phe-Ala
Saccharomyces cerevisiae
-
pH 6.5
96.7
furylacryloyl-Phe-Gly
Saccharomyces cerevisiae
-
pH 6.5
61.7
Furylacryloyl-Phe-Gly-OH
Saccharomyces cerevisiae
-
wild-type enzyme
81.7
furylacryloyl-Phe-Leu
Saccharomyces cerevisiae
-
pH 6.5
24.2
Furylacryloyl-Phe-OGly-OH
Saccharomyces cerevisiae
-
wild-type enzyme
183
furylacryloyl-Phe-OMe
Saccharomyces cerevisiae
-
pH 7.5
108
furylacryloyl-Phe-Val
Saccharomyces cerevisiae
-
pH 6.5
21.2
Hippuryl-beta-phenyl lactate
Saccharomyces cerevisiae
-
-
additional information
additional information
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
260
acetyl-D-Phe
-
hydrolysis of benzyloxycarbonyl-Phe-Leu
30
acetyl-D-Phe ethyl ester
-
hydrolysis of benzyloxycarbonyl-Phe-Leu
12
acetyl-D-Phe-ethyl ester
-
esterase activity
20 - 25
Acetyl-Phe
1.3 - 1.7
benzyloxycarbonyl-D-Phe
0.2 - 0.3
benzyloxycarbonyl-D-Phe-D-Leu
0.6 - 0.7
Benzyloxycarbonyl-Phe
2.6 - 5.7
beta-phenyl-L-lactate
1.9 - 4.5
Beta-phenylpropionate
0.000021
carboxypeptidase Y inhibitor IC
-
pH 4.7, hydrolysis of
-
24
D-Phe
-
hydrolysis of benzyloxycarbonyl-Phe-Leu
5.3 - 11
D-Phe ethyl ester
0.9 - 2.6
Leu-Phe
0.4 - 3.9
Phe
8.3 - 9.1
Phe ethyl ester
3.1 - 8
trans-cinnamate
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0078
(3R)-3-([(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000057
(3S)-3-(([1-(2-chlorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000357
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methoxyphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000038
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000252
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(3-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000274
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(4-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000375
(3S)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-[2-(trifluoromethyl)phenyl]propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.0001
(3S)-3-(([1-(3-chlorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000059
(3S)-3-(([1-(3-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000087
(3S)-3-(([1-(4-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.00011
(3S)-3-(([4-chloro-1-(2-fluorophenyl)-5-methoxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.00003
(3S)-3-(([4-fluoro-1-(2-fluorophenyl)-5-methoxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.00006
(3S)-3-(2,4-dichlorophenyl)-3-([[5-(3,3-dimethyl-2-oxobutoxy)-1-(2-fluorophenyl)-1H-pyrazol-3-yl]carbonyl]amino)propanoic acid
Homo sapiens
-
pH 6, 37C
0.000005 - 0.00005
(3S)-3-(2,4-dichlorophenyl)-3-[([1-(2-fluorophenyl)-5-[(2R)-2-hydroxy-3,3-dimethylbutoxy]-1H-pyrazol-3-yl]carbonyl)amino]propanoic acid
0.00007
(3S)-3-(2,4-dichlorophenyl)-3-[([1-(2-fluorophenyl)-5-[(2S)-2-hydroxy-3,3-dimethylbutoxy]-1H-pyrazol-3-yl]carbonyl)amino]propanoic acid
Homo sapiens
-
pH 6, 37C
0.00011
(3S)-3-([(4-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.0001
(3S)-3-([(4-methoxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.00007
(3S)-3-([(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000003
(3S)-3-([[5-(3,3-dimethyl-2-oxobutoxy)-1-(2-fluorophenyl)-1H-pyrazol-3-yl]carbonyl]amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
0.000015
(3S)-3-([[5-(cyclopropylmethoxy)-1-(2-fluorophenyl)-1H-pyrazol-3-yl]carbonyl]amino)-3-(2-methylphenyl)propanoic acid
0.00001
(3S)-3-[([1-(2-fluorophenyl)-5-[(2S)-2-hydroxy-3,3-dimethylbutoxy]-1H-pyrazol-3-yl]carbonyl)amino]-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
0.0006
3,4-dichloroisocoumarin
Homo sapiens
-
-
0.00538
3-(([1-(2,5-dimethylphenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(4-fluorophenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000226
3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-phenylpropanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000026
3-(([1-(2-fluorophenyl)-5-methoxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2-methylphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.03
3-(([1-(4-chlorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)-3-(2,5-dichlorophenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.00012
3-(2,3-dichlorophenyl)-3-(([1-(2,4-difluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.0001
3-(2,3-dichlorophenyl)-3-([(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000025
3-(4'-fluorobiphenyl-4-yl)-3-([[1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl]amino)propanoic acid
Homo sapiens
-
pH 6, 37C
0.000076
3-(5-fluoro-2-methylphenyl)-3-(([1-(2-fluorophenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl)amino)propanoic acid
Homo sapiens
-
pH 6, 37C
-
0.000803
3-(biphenyl-4-yl)-3-([[1-(2,5-dimethylphenyl)-5-hydroxy-1H-pyrazol-3-yl]carbonyl]amino)propanoic acid
Homo sapiens
-
pH 6, 37C
0.0002
3-[[(5-hydroxy-1-phenyl-1H-pyrazol-3-yl)carbonyl]amino]-3-(4-phenoxyphenyl)propanoic acid
Homo sapiens
-
pH 6, 37C
0.0022 - 0.1
chymostatin
0.01
diisopropyl fluorophosphate
Homo sapiens
-
-
0.00002 - 0.002
ebelactone B
0.0014 - 0.048
lactacystin
0.000002 - 0.000099
omuralide
0.72
Phe-Asn-Arg-Ala-Val-Asp
Homo sapiens
-
in 50 mM Tris-HCl and 0.1 M NaCl (pH 7.5)
0.63
Phe-Asn-Arg-Ala-Val-Val
Homo sapiens
-
in 50 mM Tris-HCl and 0.1 M NaCl (pH 7.5)
0.91
Phe-Asn-Arg-Pro-Val-Asp
Homo sapiens
-
in 50 mM Tris-HCl and 0.1 M NaCl (pH 7.5)
0.71
Phe-Asn-Arg-Pro-Val-Val
Homo sapiens
-
in 50 mM Tris-HCl and 0.1 M NaCl (pH 7.5)
0.0041 - 0.1
piperastatin A
0.029 - 0.1
poststatin
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
isoenzyme MpiCP-2
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
isoenzyme MpiCP-2
4.2
-
hydrolysis of polyglutamic acid
4.5
-
assay at
5.3 - 5.7
Citrus sp.
-
benzyloxycarbonyl-Leu-Phe, benzyloxycarbonyl-Arg-Pro, orange peel
5.3
Citrus sp.
-
-
6 - 7
-
hydrolysis of peptides containing only neutral amino acids, e.g. benzyloxycarbonyl-Phe-Pro, benzyloxycarbonyl-Gly-Phe, benzyloxycarbonyl-Phe-Leu, benzoyl-Gly-Phe, acetyl-Phe-Leu
6
-
hydrolysis of benzoyl-Gly-Arg
7
-
around, release of C-terminal basic amino acids
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 6
Citrus sp.
-
pH 4.5: about 70% of activity maximum, pH 6: about 55% of activity maximum
5 - 6
-
pH 5.0: about 40% of maximal activity, pH 6.0: about 40% of maximal activity, hydrolysis of furyacryloyl-Phe-Ala
5 - 7
-
pH 5.0: about 45% of maximal activity, pH 7.0: about 50% of maximal activity, hydrolysis of hippuryl-L-beta-phenyllactate
5 - 9
-
pH 5.0: about 45% of maximal activity, pH 9.0: about 75% of maximal activity, hydrolysis of acetyl-Phe ethyl ester
5.5 - 8
-
pH 5.5: about 50% of maximal activity, pH 8.0: about 35% of maximal activity, hydrolysis of benzoyl-Tyr-4-nitroanilide
5.5 - 8.5
-
pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, hydrolysis of benzyloxycarbonyl-Phe-NH2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
assay at
27
-
assay at
30
-
above 30C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 55
-
30C: about 45% of maximal activity, 55C: 75% of maximal activity, hydrolysis of furyacryloyl-Phe-Ala
30
Citrus sp.
-
30C: about 65% of activity maximum, 60C: about 70% of activity maximum
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
isoenzyme MpiCP-2
4.5
Citrus sp.
-
orange leaf
5.5
-
isoelectric focusing
6
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
nerve fiber
Manually annotated by BRENDA team
-
embryonic
Manually annotated by BRENDA team
-
of digestive tract
Manually annotated by BRENDA team
-
SmSCP-1 is nearly 2fold in female fat body compared to male fat body
Manually annotated by BRENDA team
-
from a galactosialidosis patient transformed with simian virus-40-adenovirus recombinant
Manually annotated by BRENDA team
Citrus sp.
-
flavedo
Manually annotated by BRENDA team
-
nerve fiber
Manually annotated by BRENDA team
-
embryonic
Manually annotated by BRENDA team
Citrus sp.
-
-
Manually annotated by BRENDA team
-
of testis
Manually annotated by BRENDA team
-
macrophage-like cells
Manually annotated by BRENDA team
-
of lung
Manually annotated by BRENDA team
a cDNA of bone marrow-derived monocyte/macrophage osteoclast progenitors is used
Manually annotated by BRENDA team
Citrus sp.
-
flavedo
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
vascular
Manually annotated by BRENDA team
-
macrophage-like cells
Manually annotated by BRENDA team
-
Leydig cells
Manually annotated by BRENDA team
additional information
-
SmSCP-1 is upregulated nearly four-fold in the female midges compared to males. SmSCP-1 mRNA is not detected in the midgut
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
small amount also present in cytosolic fraction of transfected HEK-293 cells
Manually annotated by BRENDA team
-
recombinant enzyme expressed in Escherichia coli
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
-
SDS-PAGE
36000
-
SDS-PAGE
41100 - 54700
-
SDS-PAGE, the number of subunits among phaseolins varies from 2 to 6 with a specific molecular weight for each subunit ranging from 54700 to 41100 Da depending on the cultivar investigated
42000
-
gel filtration
50000
-
proform, SDS-PAGE
51100
-
MALDI-TOF, mature protein. Enzymatic activity increases with maturation of Cathepsin A to the 51.1 kDa form
54000
-
proenzyme, SDS-PAGE
54400
-
MALDI-TOF, zymogen protein
55000
SDS-PAGE
67000
-
gel filtration
70000
-
proenzyme, SDS-PAGE
86000
Citrus sp.
-
enzyme from peel, gel filtration
100000
-
native complex, SDS-PAGE
125000
-
isoenzyme MpiCP-1, gel filtration
126000
Citrus sp.
-
enzyme from peel, gel filtration
132000
-
gel filtration
135000
-
SDS-PAGE
150000
-
SDS-PAGE
175000
Citrus sp.
-
enzyme from leaf, gel filtration
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotrimer
-
3 * 50000, SDS-PAGE
monomer
-
1 * 36000, SDS-PAGE; 1 * 42000, gel filtration
oligomer
-
38 * 59000, isoenzyme MpiCP-2, SDS-PAGE
trimer
-
3 * 45000, SDS-PAGE
additional information