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Literature summary for 3.4.16.5 extracted from

  • Mima, J.; Jung, G.; Onizuka, T.; Ueno, H.; Hayashi, R.
    Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y (2002), Eur. J. Biochem., 269, 3220-3225.
    View publication on PubMed
Search for more literature for 3.4.16.5

Protein Variants

Protein Variants Comment Organism
C341F reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu, benzyloxycarbonyl-Gly-Phe and benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae
C341G reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu and benzyloxycarbonyl-Gly-Phe, somewhat increased ratio with benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae
C341S reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu, benzyloxycarbonyl-Gly-Phe and benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae
C341V reduced ratio of turnover number to Km-value for the mutant enzyme compared to wild-type enzyme with the substrates benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Phe-Leu, benzyloxycarbonyl-Gly-Phe and benzyloxycarbonyl-Ala-Phe Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
0.16
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
0.19
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.26
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
0.32
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
0.41
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.45
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
0.57
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
0.83
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.89
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
0.98
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
1.1
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, wild-type enzyme anf mutant enzyme C341V Saccharomyces cerevisiae
3
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
3.2
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
3.7
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
4.2
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
5.2
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
6.7
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
8.4
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
9.1
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
19
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Gly-beta-phenyllactate + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
Benzyloxycarbonyl-Ala-Phe + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Ala + Phe
-
 ?
Benzyloxycarbonyl-Gly-Leu + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Leu
-
 ?
Benzyloxycarbonyl-Gly-Phe + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Phe
-
 ?
benzyloxycarbonyl-Phe-Leu + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Leu
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
0.467
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
0.96
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
1.1
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
1.2
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
1.4
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
1.9
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
2.1
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341D Saccharomyces cerevisiae
2.2
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
2.3
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
2.4
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
3.2
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
4.6
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
5.5
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C, mutant enzyme C341F Saccharomyces cerevisiae
7
-
 Benzyloxycarbonyl-Gly-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
8
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341V Saccharomyces cerevisiae
15
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
18
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
92
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
93
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341S Saccharomyces cerevisiae
95
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, mutant enzyme C341G Saccharomyces cerevisiae
150
-
 Benzyloxycarbonyl-Ala-Phe pH 6.5, 25°C, wild-type enzyme Saccharomyces cerevisiae