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Literature summary for 3.4.16.5 extracted from

  • Hayashi, R.; Bai, Y.; Hata, T.
    Kinetic studies of carboxypeptidase Y. I. Kinetic parameters for the hydrolysis of synthetic substrates (1975), J. Biochem., 77, 69-79.
    View publication on PubMed
Search for more literature for 3.4.16.5

Inhibitors

Inhibitors Comment Organism Structure
acetyl-D-Phe ethyl ester
-
 Saccharomyces cerevisiae
benzyloxycarbonyl-D-Phe
-
 Saccharomyces cerevisiae
benzyloxycarbonyl-D-Phe-D-Leu
-
 Saccharomyces cerevisiae
benzyloxycarbonyl-Phe
-
 Saccharomyces cerevisiae
dimethylformamide
-
 Saccharomyces cerevisiae
ethanol
-
 Saccharomyces cerevisiae
L-amino acids competitive Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 benzyloxycarbonyl-Glu-Phe pH 6.5, 25°C Saccharomyces cerevisiae
0.068
-
 benzyloxycarbonyl-Leu-Leu pH 6.5, 25°C Saccharomyces cerevisiae
0.1
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C Saccharomyces cerevisiae
0.1
-
 Benzyloxycarbonyl-His-Phe pH 6.5, 25°C Saccharomyces cerevisiae
0.1
-
 benzyloxycarbonyl-Leu-Phe pH 6.5, 25°C Saccharomyces cerevisiae
0.13
-
 benzyloxycarbonyl-Glu-Phe pH 5.5, 25°C Saccharomyces cerevisiae
0.14
-
 Benzyloxycarbonyl-Glu-Tyr pH 5.0, 25°C Saccharomyces cerevisiae
0.23
-
 Benzyloxycarbonyl-His-Phe pH 7.0, 25°C Saccharomyces cerevisiae
0.36
-
 benzyloxycarbonyl-Phe-Glu pH 4.5, 25°C Saccharomyces cerevisiae
0.38
-
 Benzyloxycarbonyl-His-Phe pH 7.5, 25°C Saccharomyces cerevisiae
0.41
-
 benzyloxycarbonyl-Phe-Glu pH 5.5, 25°C Saccharomyces cerevisiae
0.45
-
 benzoyl-Gly-beta-phenyllactate pH 6.0, 25°C Saccharomyces cerevisiae
0.5
-
 benzyloxycarbonyl-Leu-Phe pH 6.5, 25°C Saccharomyces cerevisiae
0.5
-
 Benzyloxycarbonyl-Glu-Tyr pH 5.5, 25°C Saccharomyces cerevisiae
0.56
-
 benzyloxycarbonyl-Phe-Ala pH 6.5, 25°C Saccharomyces cerevisiae
0.67
-
 benzyloxycarbonyl-Phe-Pro pH 6.5, 25°C Saccharomyces cerevisiae
0.74
-
 Acetyl-Phe-Leu pH 6.5, 25°C Saccharomyces cerevisiae
0.83
-
 benzyloxycarbonyl-His-Tyr pH 5.7, 25°C Saccharomyces cerevisiae
0.9
-
 benzyloxycarbonyl-Phe-His pH 5.5, 25°C Saccharomyces cerevisiae
1.15
-
 Benzyloxycarbonyl-Gly-Glu pH 4.5, 25°C Saccharomyces cerevisiae
1.2
-
 acetyl-Phe ethyl ester pH 8.0, 25°C Saccharomyces cerevisiae
1.28
-
 acetyl-Phe ethyl ester pH 6.5, 25°C Saccharomyces cerevisiae
1.33
-
 benzyloxycarbonyl-His-Tyr pH 6.5, 25°C Saccharomyces cerevisiae
1.7
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C Saccharomyces cerevisiae
1.75
-
 benzyloxycarbonyl-Gly-Gly pH 6.5, 25°C Saccharomyces cerevisiae
1.82
-
 benzyloxycarbonyl-His-Tyr pH 7.5, 25°C Saccharomyces cerevisiae
2.4
-
 Acetyl-Tyr-ethyl ester pH 8.0, 25°C Saccharomyces cerevisiae
3
-
 Benzyloxycarbonyl-Glu-Tyr pH 6.0, 25°C Saccharomyces cerevisiae
3.2
-
 benzyloxycarbonyl-Phe-His pH 6.5, 25°C Saccharomyces cerevisiae
3.26
-
 Benzyloxycarbonyl-Ala-Ala pH 6.5, 25°C Saccharomyces cerevisiae
3.3
-
 benzyloxycarbonyl-Glu-Phe pH 6.5, 25°C Saccharomyces cerevisiae
4
-
 benzyloxycarbonyl-Phe-Gly pH 6.5, 25°C Saccharomyces cerevisiae
7.7
-
 benzoyl-Gly-Phe pH 6.5, 25°C Saccharomyces cerevisiae
9
-
 benzyloxycarbonyl-Phe-beta-Ala pH 6.5, 25°C Saccharomyces cerevisiae
9.09
-
 Benzyloxycarbonyl-Gly-Glu pH 5.5, 25°C Saccharomyces cerevisiae
10
-
 benzyloxycarbonyl-Phe-NH2 pH 8.0, 25°C Saccharomyces cerevisiae
10
-
 benzoyl-Gly-Arg pH 6.5, 25°C Saccharomyces cerevisiae
11
-
 benzyloxycarbonyl-Phe-NH2 pH 7.0, 25°C Saccharomyces cerevisiae
15
-
 benzyloxycarbonyl-Phe-NH2 pH 6.0, 25°C Saccharomyces cerevisiae
16.5
-
 benzyloxycarbonyl-Phe-Glu pH 6.5, 25°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 baker's yeast
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Phe ethyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
acetyl-Phe-Leu + H2O
-
 Saccharomyces cerevisiae acetyl-Phe + Leu
-
 ?
Acetyl-Tyr ethyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
acetyl-Tyr-ethyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
benzoyl-Gly-Arg + H2O
-
 Saccharomyces cerevisiae benzoyl-Gly + Arg
-
 ?
benzoyl-Gly-beta-phenyllactate + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
benzoyl-Gly-Phe + H2O
-
 Saccharomyces cerevisiae benzoyl-Gly + Phe
-
 ?
benzoyl-L-Tyr-4-nitroanilide + H2O
-
 Saccharomyces cerevisiae benzoyl-Tyr + 4-nitroaniline
-
 ?
benzoyl-Tyr-4-nitroanilide + H2O
-
 Saccharomyces cerevisiae benzoyl-Tyr + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Ala + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Ala + Ala
-
 ?
benzyloxycarbonyl-Glu-Phe + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Glu + Phe
-
 ?
Benzyloxycarbonyl-Glu-Tyr + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Glu + Tyr
-
 ?
benzyloxycarbonyl-Gly-Glu + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Gly + Glu
-
 ?
benzyloxycarbonyl-Gly-Gly + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Gly + Gly
-
 ?
benzyloxycarbonyl-Gly-Gly-Phe + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Gly-Gly + Phe
-
 ?
Benzyloxycarbonyl-Gly-Phe + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Phe
-
 ?
benzyloxycarbonyl-His-Phe + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-His + Phe
-
 ?
benzyloxycarbonyl-His-Tyr + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-His + Tyr
-
 ?
benzyloxycarbonyl-Leu-Leu + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Leu + Leu
-
 ?
benzyloxycarbonyl-Leu-Phe + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Leu + Phe
-
 ?
benzyloxycarbonyl-Phe-Ala + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Ala
-
 ?
benzyloxycarbonyl-Phe-beta-Ala + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + beta-Ala
-
 ?
benzyloxycarbonyl-Phe-Glu + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Glu
-
 ?
benzyloxycarbonyl-Phe-Gly + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Gly
-
 ?
benzyloxycarbonyl-Phe-His + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + His
-
 ?
benzyloxycarbonyl-Phe-Leu + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Leu
-
 ?
benzyloxycarbonyl-Phe-NH2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
benzyloxycarbonyl-Phe-Phe + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Phe
-
 ?
benzyloxycarbonyl-Phe-Pro + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Pro
-
 ?
additional information overview: synthetic substrates Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
 Benzyloxycarbonyl-Gly-Glu pH 4.5, 25°C Saccharomyces cerevisiae
0.46
-
 Benzyloxycarbonyl-Gly-Glu pH 5.5, 25°C Saccharomyces cerevisiae
0.72
-
 Benzyloxycarbonyl-His-Phe pH 6.5, 25°C Saccharomyces cerevisiae
1.44
-
 benzoyl-Gly-Arg pH 6.5, 25°C Saccharomyces cerevisiae
1.82
-
 Benzyloxycarbonyl-His-Phe pH 7.0, 25°C Saccharomyces cerevisiae
2.05
-
 Benzyloxycarbonyl-His-Phe pH 7.5, 25°C Saccharomyces cerevisiae
2.18
-
 benzyloxycarbonyl-Gly-Gly pH 6.5, 25°C Saccharomyces cerevisiae
2.2
-
 Benzyloxycarbonyl-Gly-Phe pH 6.5, 25°C Saccharomyces cerevisiae
2.2
-
 benzyloxycarbonyl-Phe-NH2 pH 7.0, 25°C Saccharomyces cerevisiae
2.8
-
 benzyloxycarbonyl-Phe-NH2 pH 6.0, 25°C Saccharomyces cerevisiae
3
-
 benzyloxycarbonyl-Phe-His pH 5.5, 25°C Saccharomyces cerevisiae
3.8
-
 benzoyl-Gly-Phe pH 6.5, 25°C Saccharomyces cerevisiae
5.3
-
 benzyloxycarbonyl-Phe-NH2 pH 8.0, 25°C Saccharomyces cerevisiae
6.85
-
 benzyloxycarbonyl-Leu-Leu pH 6.5, 25°C Saccharomyces cerevisiae
8.2
-
 benzyloxycarbonyl-His-Tyr pH 6.5 or pH 7.5, 25°C Saccharomyces cerevisiae
10.7
-
 benzyloxycarbonyl-His-Tyr pH 5.7, 25°C Saccharomyces cerevisiae
11.6
-
 benzyloxycarbonyl-Phe-His pH 6.5, 25°C Saccharomyces cerevisiae
13.3
-
 benzyloxycarbonyl-Glu-Phe pH 6.5, 25°C Saccharomyces cerevisiae
15.5
-
 benzyloxycarbonyl-Glu-Phe pH 4.5, 25°C Saccharomyces cerevisiae
16.8
-
 benzyloxycarbonyl-Glu-Phe pH 5.5, 25°C Saccharomyces cerevisiae
18.8
-
 Benzyloxycarbonyl-Glu-Tyr pH 5.0, 25°C Saccharomyces cerevisiae
19
-
 Benzyloxycarbonyl-Glu-Tyr pH 5.5, 25°C Saccharomyces cerevisiae
21.2
-
 benzoyl-Gly-beta-phenyllactate pH 6.0, 25°C Saccharomyces cerevisiae
23
-
 benzyloxycarbonyl-Phe-Pro pH 6.5, 25°C Saccharomyces cerevisiae
23.8
-
 Benzyloxycarbonyl-Glu-Tyr pH 6.0, 25°C Saccharomyces cerevisiae
24.5
-
 benzyloxycarbonyl-Phe-Glu pH 4.5, 25°C Saccharomyces cerevisiae
26.2
-
 benzyloxycarbonyl-Phe-Glu pH 5.5, 25°C Saccharomyces cerevisiae
36.1
-
 Acetyl-Phe-Leu pH 6.5, 25°C Saccharomyces cerevisiae
46
-
 benzyloxycarbonyl-Leu-Phe pH 6.5, 25°C Saccharomyces cerevisiae
66
-
 benzyloxycarbonyl-Phe-beta-Ala pH 6.5, 25°C Saccharomyces cerevisiae
69.4
-
 Benzyloxycarbonyl-Ala-Ala pH 6.5, 25°C Saccharomyces cerevisiae
96.5
-
 benzyloxycarbonyl-Phe-Glu pH 6.5, 25°C Saccharomyces cerevisiae
105
-
 Acetyl-Tyr ethyl ester pH 8.0, 25°C Saccharomyces cerevisiae
120
-
 benzyloxycarbonyl-Phe-Ala pH 6.5, 25°C Saccharomyces cerevisiae
120
-
 acetyl-Phe ethyl ester pH 8.0, 25°C Saccharomyces cerevisiae
122
-
 acetyl-Phe ethyl ester pH 6.5, 25°C Saccharomyces cerevisiae
130
-
 Benzyloxycarbonyl-Phe-Leu pH 6.5, 25°C Saccharomyces cerevisiae
140
-
 benzyloxycarbonyl-Phe-Gly pH 6.5, 25°C Saccharomyces cerevisiae
420
-
 benzyloxycarbonyl-Phe-Phe pH 6.5, 25°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 hydrolysis of polyglutamic acid Saccharomyces cerevisiae
5.5
-
 peptides containing an acidic amino acid, e.g. benzyloxycarbonyl-Phe-Glu, and benzyloxycarbonyl-Glu-Phe Saccharomyces cerevisiae
6 7 hydrolysis of peptides containing only neutral amino acids, e.g. benzyloxycarbonyl-Phe-Pro, benzyloxycarbonyl-Gly-Phe, benzyloxycarbonyl-Phe-Leu, benzoyl-Gly-Phe, acetyl-Phe-Leu Saccharomyces cerevisiae
6
-
 hydrolysis of benzoyl-Gly-Arg Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 45% of maximal activity, pH 9.0: about 75% of maximal activity, hydrolysis of acetyl-Phe ethyl ester Saccharomyces cerevisiae
5 7 pH 5.0: about 45% of maximal activity, pH 7.0: about 50% of maximal activity, hydrolysis of hippuryl-L-beta-phenyllactate Saccharomyces cerevisiae
5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, hydrolysis of benzyloxycarbonyl-Phe-NH2 Saccharomyces cerevisiae
5.5 8 pH 5.5: about 50% of maximal activity, pH 8.0: about 35% of maximal activity, hydrolysis of benzoyl-Tyr-4-nitroanilide Saccharomyces cerevisiae