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Literature summary for 3.4.16.5 extracted from

  • Masuhara, M.; Sato, T.; Hada, N.; Hakeda, Y.
    Protective protein/cathepsin A down-regulates osteoclastogenesis by associating with and degrading NF-kappaB p50/p65 (2009), J. Bone Miner. Metab., 27, 46-56.
    View publication on PubMed
Search for more literature for 3.4.16.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK293 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information a yeast two-hybrid screening using a cDNA library of osteoclast precursors discloses PPCA as a binding partner of NF-kappaB p50/p65. Forced expression of PPCA with p50/p65 in HEK293 cells decreases both the level of p50/p65 proteins and the transcriptional activity. Overexpression of PPCA causes the disappearance of p50/p65 in both the lysosomal and cytosolic fractions. PPCA is expressed throughout osteoclastogenesis,and the expression is slightly up-regulated by nuclear factor (NF)-kappaB ligand signaling. Knockdown of PPCA in osteoclast precursors with PPCA siRNA stimulates binding of nuclear proteins to oligonucleotides containing an NF-kappaB binding motif and increases osteoclastogenesis Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol small amount also present in cytosolic fraction of transfected HEK-293 cells Mus musculus 5829
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lysosome in transfected HEK-293 cells Mus musculus 5764
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Organism

Organism UniProt Comment Textmining
Mus musculus P16675
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-

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow cell line
-
 Mus musculus
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osteoclast a cDNA of bone marrow-derived monocyte/macrophage osteoclast progenitors is used Mus musculus
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Synonyms

Synonyms Comment Organism
PpcA
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 Mus musculus
protective protein/cathepsin A
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 Mus musculus