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Literature summary for 3.4.16.5 extracted from
- Purification and characterization of a high molecular mass serine carboxypeptidase from Monascus pilosus (2004), J. Ind. Microbiol. Biotechnol., 31, 572-580.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(p-nitrophenoxy)propane | 1 mM, 14% inhibition, isoenzyme MpiCP-2 | Monascus pilosus |  |
| 2,2'-dipyridyl | 1 mM, 21% inhibition, isoenzyme MpiCP-1; 1 mM, 22% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| 2-mercaptoethanol | 1 mM, 13% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| chymostatin | 1 mM, 36% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| diisopropylfluorophosphate | 1 mM, 39% inhibition, isoenzyme MpiCP-1; 1 mM, 67% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| E-64 | 1 mM, 10% inhibition, isoenzyme MpiCP-2; 1 mM, 22% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| EDTA | 1 mM, 38% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| Elastatinal | 1 mM, 46% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| leupeptin | 1 mM, 24% inhibition, isoenzyme MpiCP-1; 1 mM, 60% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| Monoiodoacetic acid | 1 mM, 14% inhibition; 1 mM, 25% inhibition | Monascus pilosus | |
| additional information | no inhibition with 1 mM EDTA or chymostatin | Monascus pilosus | |
| Nalpha-tosyl-L-Lys-chloromethyl ketone | 1 mM, 13% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| Nalpha-tosyl-L-Phe-chloromethyl ketone | 1 mM, 12% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| PCMB | 1 mM, 28% inhibition, isoenzyme MpiCP-1; 1 mM, 97% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| pepstatin A | 1 mM, 11% inhibition, isoenzyme MpiCP-2; 1 mM, 19% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| Phenylmethylsulfonylfluoride | 1 mM, 61% inhibition, isoenzyme MpiCP-1; 1 mM, complete inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| phosphoramidon | 1 mM, 17% inhibition, isoenzyme MpiCP-1; 1 mM, 41% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| piperastatin A | 1 mM, 17% inhibition, isoenzyme MpiCP-2; 1 mM, 87% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| tosylphenylalanylchloromethane | 1 mM, 35% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.33 | - |
benzyloxycarbonyl-Tyr-Glu | pH 4.0, 37°C, isoenzyme MpiCP-1 | Monascus pilosus | |
| 1.55 | - |
benzyloxycarbonyl-Tyr-Glu | pH 3.5, 37°C, isoenzyme MpiCP-2 | Monascus pilosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM, 18% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| CdCl2 | 1 mM, 18% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| CoCl2 | 1 mM, 23% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| CuCl | 1 mM, 33% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| CuCl | 1 mM, 84% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| CuCl2 | 1 mM, 36% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| CuCl2 | 1 mM, 73% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| FeCl2 | 1 mM, 35% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| FeCl2 | 1 mM, 73% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| FeCl3 | 1 mM, 37% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| FeCl3 | 1 mM, 86% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| HgCl2 | 1 mM, complete inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| MgCl2 | 1 mM, 15% inhibition, isoenzyme MpiCP-1 | Monascus pilosus | |
| NiCl2 | 1 mM, 12% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| PbCl2 | 1 mM, 47% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| SnCl2 | 1 mM, 47% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
| ZnSO4 | 1 mM, 46% inhibition, isoenzyme MpiCP-2 | Monascus pilosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
38 * 59000, isoenzyme MpiCP-2, SDS-PAGE | Monascus pilosus |
| 61000 | - |
2 * 61000, isoenzyme MpiCP-1, SDS-PAGE | Monascus pilosus |
| 125000 | - |
isoenzyme MpiCP-1, gel filtration | Monascus pilosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monascus pilosus | - |
isoenzyme MpiCP-1 | - |
| Monascus pilosus | - |
isoenzyme MpiCP-2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 11% carbohydrate, isoenzyme MpiCP-1 | Monascus pilosus |
| glycoprotein | 33% carbohydrate, isoenzyme MpiCP-2 | Monascus pilosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme MpiCP-1 | Monascus pilosus |
| isoenzyme MpiCP-2 | Monascus pilosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.91 | - |
- |
Monascus pilosus |
| 4 | - |
isoenzyme MpiCP-2 | Monascus pilosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Gly-Arg + H2O | isoenzyme MpiCP-2 | Monascus pilosus | benzoyl-Gly + Arg | - |
? | |
| benzoyl-Gly-Lys + H2O | isoenzyme MpiCP-2 | Monascus pilosus | benzoyl-Gly + Lys | - |
? | |
| benzyloxycarbonyl-Ala-Glu + H2O | isoenzyme MpiCP-1 | Monascus pilosus | benzyloxycarbonyl-Ala + Glu | - |
? | |
| benzyloxycarbonyl-Ala-Glu + H2O | isoenzyme MpiCP-2 | Monascus pilosus | benzyloxycarbonyl-Ala + Glu | - |
? | |
| benzyloxycarbonyl-Glu-Phe + H2O | isoenzyme MpiCP-1 | Monascus pilosus | benzyloxycarbonyl-Glu + Phe | - |
? | |
| Benzyloxycarbonyl-Glu-Tyr + H2O | isoenzyme MpiCP-1 | Monascus pilosus | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| Benzyloxycarbonyl-Glu-Tyr + H2O | isoenzyme MpiCP-2 | Monascus pilosus | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| benzyloxycarbonyl-Phe-Ala + H2O | isoenzyme MpiCP-1 | Monascus pilosus | benzyloxycarbonyl-Phe + Ala | - |
? | |
| benzyloxycarbonyl-Phe-Ala + H2O | isoenzyme MpiCP-2 | Monascus pilosus | benzyloxycarbonyl-Phe + Ala | - |
? | |
| benzyloxycarbonyl-Phe-Gly + H2O | isoenzyme MpiCP-2 | Monascus pilosus | benzyloxycarbonyl-Phe + Gly | - |
? | |
| benzyloxycarbonyl-Phe-Leu + H2O | isoenzyme MpiCP-1 | Monascus pilosus | benzyloxycarbonyl-Phe + Leu | - |
? | |
| benzyloxycarbonyl-Phe-Leu + H2O | isoenzyme MpiCP-2 | Monascus pilosus | benzyloxycarbonyl-Phe + Leu | - |
? | |
| benzyloxycarbonyl-Pro-Leu-Gly + H2O | isoenzyme MpiCP-2 | Monascus pilosus | benzyloxycarbonyl-Pro-Leu + Gly | - |
? | |
| benzyloxycarbonyl-Tyr-Glu + H2O | preferred substrate for isoenzyme MpiCP-1 | Monascus pilosus | benzyloxycarbonyl-Tyr + Glu | - |
? | |
| benzyloxycarbonyl-Tyr-Glu + H2O | preferred substrate for isoenzyme MpiCP-2 | Monascus pilosus | benzyloxycarbonyl-Tyr + Glu | - |
? | |
| additional information | no activity with benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Gly-Phe, benzyloxycarbonyl-Gly-Pro, benzyloxycarbonyl-Gly-Pro-Leu, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, benzoyl-Gly-Lys, Leu-Gly-Gly | Monascus pilosus | ? | - |
? | |
| additional information | no activity with benzyloxycarbonylGlu-Phe, benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Gly-Phe, benzyloxycarbonyl-Gly-Pro, benzyloxycarbonyl-Gly-Pro-Leu, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, Leu-Gly-Gly | Monascus pilosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 61000, isoenzyme MpiCP-1, SDS-PAGE | Monascus pilosus |
| oligomer | 38 * 59000, isoenzyme MpiCP-2, SDS-PAGE | Monascus pilosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MpiCP-1 | - |
Monascus pilosus |
| MpiCP-2 | - |
Monascus pilosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
isoenzyme MpiCP-1 | Monascus pilosus |
| 50 | - |
isoenzyme MpiCP-2 | Monascus pilosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.0, 15 min, isoenzyme MpiCP-2 is stable up to | Monascus pilosus |
| 55 | - |
pH 6.0, 15 min, isoenzyme MpiCP-1 is stable | Monascus pilosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 723 | - |
benzyloxycarbonyl-Tyr-Glu | pH 4.0, 37°C, isoenzyme MpiCP-1 | Monascus pilosus | |
| 2039 | - |
benzyloxycarbonyl-Tyr-Glu | pH 3.5, 37°C, isoenzyme MpiCP-2 | Monascus pilosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
isoenzyme MpiCP-2 | Monascus pilosus |
| 4 | - |
isoenzyme MpiCP-1 | Monascus pilosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | 37°C, 1 h, isoenzyme MpiCP-1 is stable | Monascus pilosus |
| 5.5 | 6.6 | 37°C, 1 h, isoenzyme MpiCP-2 is stable up to | Monascus pilosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Monascus pilosus | isoenzyme MpiCP-1 | - |
3.7 |
| Monascus pilosus | isoenzyme MpiCP-2 | - |
4 |
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