Information on EC 3.2.1.51 - alpha-L-fucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.51
-
RECOMMENDED NAME
GeneOntology No.
alpha-L-fucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an alpha-L-fucoside + H2O = L-fucose + an alcohol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
-
-
-
-
transglycosylation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
degradation of hexoses
-
-
Other glycan degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-L-fucoside fucohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9037-65-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Bacillus fulminans
-
-
-
Manually annotated by BRENDA team
strain Bt2970
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
putative alpha-L-fucosidase, precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
canine
-
-
Manually annotated by BRENDA team
putative uncharacterized protein; CB15, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
guinea pig
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
probable glycosyl hydrolase; strain 13, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
Chinese hamster ovary cells
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
abalone
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
B3W8U6, B3WB08, B3WBB5
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
comparative and phylogenetic analysis of alpha-L-fucosidase genes; putative uncharacterized protein; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
Syrian hamster
-
-
Manually annotated by BRENDA team
Microscilla sp.
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
no activity in Trichomonas foetus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
marine mollusk
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
alpha-L-fucosidase, putative; W83, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
almond
-
-
Manually annotated by BRENDA team
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
SH1, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
strain R6, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
strain TIGR4 comparative and phylogenetic analysis of alpha-L-fucosidase genes
Q97NA6
SwissProt
Manually annotated by BRENDA team
putative glycosyl hydrolase; strain A3(2), comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
putative glycosyl hydrolase; strain A3(2), comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
strain 142, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
OH11242
-
-
Manually annotated by BRENDA team
comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Manually annotated by BRENDA team
strain Y5
-
-
Manually annotated by BRENDA team
strain Y5
-
-
Manually annotated by BRENDA team
alpha-L-fucosidase family protein; ATCC35405, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Venus mercenaria
-
-
-
Manually annotated by BRENDA team
strain pv. Citri str.306, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
pv. campestris str. 8004, comparative and phylogenetic analysis of alpha-L-fucosidase genes
Q4UXD3
SwissProt
Manually annotated by BRENDA team
Xenopus (Silurana) tropicalis
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
enzyme is involved in gamete recognition. Recombinant enzyme interacts with alpha-L-fucose residues present on the micropyle of the Drosophila melanogaster eggshell, confirming that it is a good candidate as receptor involved in gamete interactions in fruit fly
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
show the reaction diagram
2-chloro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-chloro-4-nitrophenol + L-fucose
show the reaction diagram
-
pH 6.8, 37C
-
-
?
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride + H2O
fluoride + 2-deoxy-2-fluoro-alpha-L-fucose
show the reaction diagram
-
-
-
-
2-fluoro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-fluoro-4-nitrophenol + L-fucose
show the reaction diagram
-
pH 6.8, 37C
-
-
?
2-methyl-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-methyl-4-nitrophenol + L-fucose
show the reaction diagram
-
pH 6.8, 37C
-
-
?
2-nitrophenyl alpha-L-fucopyranoside + H2O
2-nitrophenol + alpha-L-fucopyranose
show the reaction diagram
B3W8U6, B3WB08, B3WBB5
-
-
-
?
2alpha-fucosyl-lactitol + H2O
?
show the reaction diagram
-
-
-
-
-
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
show the reaction diagram
4-methylumbelliferyl alpha-L-fucopyranoside
4-methylumbelliferone + alpha-L-fucose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
show the reaction diagram
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
show the reaction diagram
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
methylumbelliferone + alpha-L-fucose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-fucoside
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
-
sodium acetate buffer 50 mM, pH 5.0, 37C
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside
?
show the reaction diagram
-
assay at pH 4.5, 37C for 3 h, reaction stopped by addition of borate buffer
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
show the reaction diagram
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
show the reaction diagram
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-fucose-(1-3)-D-GlcNAc
show the reaction diagram
-
catalytic residues Asp242, Glu58, and Glu292
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
show the reaction diagram
-
activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
alpha-L-fucopyranose + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
show the reaction diagram
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
show the reaction diagram
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
show the reaction diagram
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
show the reaction diagram
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-a-L-Fuc-O-4-NP + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-L-Fuc-(1-3)-alpha-L-Fuc-O-4-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
show the reaction diagram
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-3)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
B3W8U6, B3WB08, B3WBB5
-
-
-
?
alpha-L-Fuc-(1-4)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
B3W8U6, B3WB08, B3WBB5
-
-
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
show the reaction diagram
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-6)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
B3W8U6, B3WB08, B3WBB5
-
-
-
?
alpha-L-fucopyranosyl fluoride + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
show the reaction diagram
-
-
13% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + N-acetylglucosamine
2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranose + fluoride
show the reaction diagram
-
-
7% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
show the reaction diagram
-
-
8% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + fluoride
show the reaction diagram
-
-
34% yield based on the donor substrate
-
?
alpha-L-fucosyl fluoride + H2O
alpha-L-fucose + fluoride
show the reaction diagram
alpha-L-fucosyl fluoride + H2O
fluoride + alpha-L-fucose
show the reaction diagram
-
-
-
?
antigen H disaccharide + H2O
?
show the reaction diagram
B3W8U6, B3WB08, B3WBB5
-
-
-
?
antigen H type II trisaccharide + H2O
?
show the reaction diagram
B3W8U6, B3WB08, B3WBB5
-
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
show the reaction diagram
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
alpha-L-fucose + Fuc-beta-4-nitrophenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
alpha-L-fucose + beta-galactosyl-4-nitrophenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Xyl + H2O
alpha-L-fucose + D-xylose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
show the reaction diagram
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
show the reaction diagram
Fuc-alpha-(1-3)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
alpha-L-fucose + GlcNAc-4-nitrophenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
show the reaction diagram
-
-
-
?
fucoidan + H2O
alpha-L-fucose + ?
show the reaction diagram
-
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
show the reaction diagram
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
show the reaction diagram
gastric mucin + H2O
alpha-L-fucose + ?
show the reaction diagram
GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
show the reaction diagram
-
not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues
-
?
glycopeptide + H2O
alpha-L-fucose + ?
show the reaction diagram
glycoprotein + H2O
alpha-L-fucose + ?
show the reaction diagram
glycosphingolipid + H2O
alpha-L-fucose + ?
show the reaction diagram
Venus mercenaria
-
blood-group active types B and H
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
show the reaction diagram
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
IgG Fc peptide + H2O
alpha-L-fucose + ?
show the reaction diagram
IgG L peptide + H2O
alpha-L-fucose + ?
show the reaction diagram
-
-
-
?
lacto-N-difucohexaitol I + H2O
alpha-L-fucose + ?
show the reaction diagram
-
low activity
-
?
lacto-N-difucohexaose I + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
-
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
show the reaction diagram
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
lactoferrin + H2O
alpha-L-fucose + ?
show the reaction diagram
-
low activity
-
?
LH-beta peptic glycopeptide + H2O
alpha-L-fucose + ?
show the reaction diagram
-
-
-
?
m-nitrophenyl alpha-L-fucopyranoside + H2O
m-nitrophenol + L-fucose
show the reaction diagram
-
pH 6.8, 37C
-
-
?
Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
show the reaction diagram
-
-
-
?
meconium H-1 + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
-
-
?
meconium H-2 + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
-
-
?
meconium X-1 + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
low activity
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
show the reaction diagram
milk + H2O
alpha-L-fucose + ?
show the reaction diagram
-
human
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
show the reaction diagram
oligosaccharides + H2O
alpha-L-fucose + ?
show the reaction diagram
orosomucoid + H2O
alpha-L-fucose + ?
show the reaction diagram
-
low activity, higher activity if desialyzed
-
?
p-cyanophenyl alpha-L-fucopyranoside + H2O
p-cyanophenol + L-fucose
show the reaction diagram
-
pH 6.8, 37C
-
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
show the reaction diagram
p-nitrophenyl alpha-L-fucopyranoside + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
show the reaction diagram
-
-
29% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
show the reaction diagram
-
-
34% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + p-nitrophenol
show the reaction diagram
-
-
25% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
show the reaction diagram
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
show the reaction diagram
p-nitrophenyl-alpha-L-fucoside + H2O
L-fucose + p-nitrophenol
show the reaction diagram
the enzyme has low transglycosylation properties
-
-
?
phenyl alpha-L-fucopyranoside + H2O
phenol + L-fucose
show the reaction diagram
-
pH 6.8, 37C
-
-
?
saliva + H2O
alpha-L-fucose + ?
show the reaction diagram
stomach + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
-
-
?
submaxilary mucin trisaccharide + H2O
alpha-L-fucose + disaccharide
show the reaction diagram
-
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
show the reaction diagram
submaxillary porcine trisaccharide + H2O
alpha-L-fucose + disaccharide
show the reaction diagram
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
show the reaction diagram
Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside
show the reaction diagram
-
-
-
?
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Fucalpha(1-2)Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + H2O
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + alpha-L-fucose
show the reaction diagram
-
-
-
-
?
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT-[Fuc-alpha-(1-6)] + H2O
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT + alpha-L-fucose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
show the reaction diagram
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
show the reaction diagram
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
-
-
-
-
?
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
show the reaction diagram
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
show the reaction diagram
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
show the reaction diagram
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
show the reaction diagram
-
same yield of transglycosylation and hydrolysis products
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
show the reaction diagram
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
-
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-2)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Xyl + H2O
alpha-L-fucose + D-xylose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
show the reaction diagram
Fuc-alpha-(1-3)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
show the reaction diagram
-
-
-
?
fucoidan + H2O
alpha-L-fucose + ?
show the reaction diagram
-
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
show the reaction diagram
-
very slow hydrolysis, NaBH4-reduced oligosaccharide
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
show the reaction diagram
gastric mucin + H2O
alpha-L-fucose + ?
show the reaction diagram
glycopeptide + H2O
alpha-L-fucose + ?
show the reaction diagram
glycoprotein + H2O
alpha-L-fucose + ?
show the reaction diagram
glycosphingolipid + H2O
alpha-L-fucose + ?
show the reaction diagram
Venus mercenaria
-
blood-group active types B and H
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
show the reaction diagram
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
IgG Fc peptide + H2O
alpha-L-fucose + ?
show the reaction diagram
-
human, rabbit, horse
-
?
IgG L peptide + H2O
alpha-L-fucose + ?
show the reaction diagram
-
-
-
?
lacto-N-difucohexaitol I + H2O
alpha-L-fucose + ?
show the reaction diagram
-
low activity
-
?
lacto-N-difucohexaose I + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
-
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
show the reaction diagram
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
lactoferrin + H2O
alpha-L-fucose + ?
show the reaction diagram
-
low activity
-
?
LH-beta peptic glycopeptide + H2O
alpha-L-fucose + ?
show the reaction diagram
-
-
-
?
meconium H-1 + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
-
-
?
meconium H-2 + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
-
-
?
meconium X-1 + H2O
alpha-L-fucose + ?
show the reaction diagram
Bacillus fulminans
-
low activity
-
?
milk + H2O
alpha-L-fucose + ?
show the reaction diagram
-
human
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
show the reaction diagram
orosomucoid + H2O
alpha-L-fucose + ?
show the reaction diagram
-
low activity, higher activity if desialyzed
-
?
saliva + H2O
alpha-L-fucose + ?
show the reaction diagram
submaxillary mucin + H2O
alpha-L-fucose + ?
show the reaction diagram
submaxillary porcine trisaccharide + H2O
alpha-L-fucose + disaccharide
show the reaction diagram
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
show the reaction diagram
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT-[Fuc-alpha-(1-6)] + H2O
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT + alpha-L-fucose
show the reaction diagram
-
-
-
?
additional information
?
-
-
the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ag+
-
5 mM, complete inhibition
Cd2+
-
5 mM, about 5% inhibition
Co2+
-
5 mM, about 5% inhibition
Cu2+
-
5 mM, about 5% inhibition
Mn2+
-
22% stimulation at 5 mM
additional information
-
the addition of calcium, magnesium, cobalt, manganese, nickel, and zinc at 10 mM concentrations does not increase enzymatic rates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,3R,4R,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
(1R,2R,3R,4S,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
(2S,3R,4R,5R)-5-(3-aminopropoxy)-2-methylpiperidine-3,4-diol
-
-
(2S,3R,4R,5R,6S)-2-(hydroxymethyl)-6-methylpiperidine-3,4,5-triol
-
-
(2S,3R,4S,5R)-1-(3-aminopropyl)-2-methylpiperidine-3,4,5-triol
-
-
(2S,3R,4S,5R)-2-(propan-2-yl)piperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
(2S,3R,4S,5R)-2-isopropylpiperidine-3,4,5-triol
-
-
(2S,3R,4S,5S)-2-methyl-5-(phenylamino)pyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-(1H-benzimidazol-2-yl)-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-[(biphenyl-4-ylamino)methyl]-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(5R,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 50% inhibition
(5R,6S,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 41% inhibition
(5S,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 49% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 55% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]-4-azaspiro[2.4]heptane
-
1 mM,97 inhibition, competitive
(R)-2-aminobutanol
(S)-2-aminobutanol
1,5-dideoxy-1,5-([(2R,3R)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2R,3S,4R,5R)-2,4,5,6-tetrahydroxy-3-(sulfooxy)hexyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-[5-methoxycarbonyl-1-pentyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1,5-dideoxy-1,5-[methyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
-
complete inactivation at 22 mM
1-deoxyfuconojirimycin
-
-
1-deoxygalactonojirimycin
-
-
1-deoxymannojirimycin
-
-
1-deoxynojirimycin
-
-
2-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-(hydroxymethyl)-5-methylpyrrolidine-3,4-diol
-
competitive or uncompetitive inhibition, Ki: 0.004-26 mM, depending on conformation
2-cyclopentyl-2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)acetamide
-
-
2-cyclopentyl-2-phenyl-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-methyltetrahydro-2H-pyran-3,4-diol
-
uncompetitive inhibition, Ki: 23 mM
2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)quinoline-4-carboxamide
-
-
2-phenyl-tetrahydro-2H-pyran-3,4-diol
-
uncompetitive inhibition, Ki: 22-83 mM, depending on conformation
3-(1H-indol-3-yl)-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)propanamide
-
-
3-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]propanamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
4-aminophenyl 1-thio alpha-L-fucoside
-
weak competitive inhibition, Ki: 1.25 mM
4-chloromercuribenzoate
4-chloromercuriphenylsulfonic acid
4-hydroxy-2-methyltetrahydro-2H-pyran-3-yl acetate
-
competitive inhibition, Ki: 0.09 mM
-
4-mercuribenzoate
-
complete inhibition at 0.25 mM
5-(hydroxymethyl)pyrrolidine-2,3,4-triol
-
competitive inhibition, Ki: 0.91 mM
5-amino-5-deoxy-L-fucose
-
-
5-fluoro-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)-1H-indole-2-carboxamide
-
-
6-N-acetylamino-deoxymannojirimycin
-
-
Ag2+
-
complete inactivation at 1 mM
alpha-L-fuco-N-benzyl aminocyclopentitol
alpha-L-fucose
Ca2+
-
alpha-fucosidase I, slight inhibition at 10 mM
Cd2+
-
effective inhibition at 5 mM
D-1-deoxymannojirimycin
-
competitive inhibition, Ki: 0.03 mM
D-1-deoxyrhamnojirimycin
-
competitive inhibition, Ki: 0.011 mM
D-1-deoxytalonojirimycin
-
competitive inhibition, Ki: 0.053 mM
D-arabinose
D-fucal
-
uncompetitive inhibition, Ki: 35 mM
D-fucose
-
15% inhibition at 50 mM
D-Galactono-1,4-lactone
-
15% inhibition at 50 mM
D-galactose
D-glucono-1,5-lactone
-
30% inhibition at 50 mM
D-glucose
deoxycholate
deoxyfuconojirimycin
ethanolamine
-
strong inhibition
ethylenediaminetetraacetic acid
Bacillus fulminans
-
68% inhibition at 0.1 mM
Fe3+
-
40% inhibition at 2 mM
formaldehyde
-
57% inhibition at 160 mM
fuconojirimycin
fucose
-
-
fucosylamine
-
competitive inhibition, Ki: 0.014 mM
iodoacetamide
-
30% inhibition at 1 mM
L-1-deoxyfuconojirimycin
-
competitive inhibition, Ki: 0.00003 mM
-
L-1-deoxymannojirimycin
-
competitive inhibition, Ki: 90 mM
-
L-1-deoxyrhamnojirimycin
-
competitive inhibition, Ki: 1 mM
-
L-cysteine
L-fucal
-
competitive inhibition, best inhibitor of glycal compounds tested, Ki: 0.05 mM
-
L-fuco-nojirimycin
-
very potent, competitive inhibitor
L-fucosamine
-
-
L-fucose
-
-
L-galactono-1,4-lactone
-
30% inhibition at 50 mM
L-rhamnose
-
competitive inhibition, Ki: 140 mM
Mg2+
-
slight inhibition at 10 mM
N-ethylmaleimide
-
30% inhibition at 1 mM
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1-benzofuran-2-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1H-indole-3-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
-
p-nitrophenyl alpha-L-fucoside
-
; substrate inhibition at high concentration
Pb2+
-
effective inhibition at 5 mM
Phenol
-
noncompetitive inhibition, Ki: 112 mM
SDS
Bacillus fulminans
-
42% inhibition at 0.02%, w/v
Sn2+
-
24% inhibition at 2 mM
Tris buffer
-
-
Zn-acetate
-
more than 90% inhibition at 5 mM
additional information
-
time-associated loss in the activity of alpha-fucosidase post infection. Sialic acid release is correlated with a gradual loss in activity of the enzyme
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
azide
-
increases the rate of substrate cleavage in depending of azide concentration
bovine serum albumin
chemokine CCL1
-
1.8-fold increase of ALP-expression after 24-h stimulation
-
chemokine CCL5
-
2.2-fold increase of ALP-expression after 24-h stimulation
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CN-
-
30% activation at 10 mM
dithiothreitol
-
13% activation at 0.2 mM
ethylenediaminetetraacetic acid
-
5% activation at 10 mM
formate
-
increases the rate of substrate cleavage in depending of azide concentration
KCl
-
17% activation at 300 mM
methanol
-
linear increase in enzyme-catalyzed cleavage of p-nitrophenyl alpha-L-fucoside and fucosyl fluoride
NaCl
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small positive effect
Sodium azide
-
1M, enhanced enzyme activity, only in mutants
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.67 - 1.18
2'-fucosyllactitol
0.63 - 10.6
2'-fucosyllactose
0.063
2-chloro-4-nitrophenyl alpha-L-fucopyranoside
-
mutant E289G
2.1
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride
-
pH 5.0, 60C
0.27 - 5.2
2-nitrophenyl alpha-L-fucopyranoside
1.25 - 1.9
3'-fucosyllactose
0.06 - 0.08
4-methylumbelliferyl alpha-L-fucopyranoside
0.022 - 0.4
4-methylumbelliferyl-alpha-L-fucopyranoside
0.00325 - 0.01825
4-methylumbelliferyl-beta-fucoside
0.02 - 17
4-nitrophenyl-alpha-L-fucopyranoside
0.0287
4-nitrophenyl-alpha-L-fucoside
-
wild-type and mutant fucA1B
3
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc
-
pH 6.5, 37C
7
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
pH 6.5, 37C
0.075 - 2.5
alpha-L-fucosyl fluoride
9
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc
-
pH 6.5, 37C
2.9
Fuc-alpha-(1-2)-Gal
-
-
1.56
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc
-
-
0.52
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol
-
-
1.63
Fuc-alpha-(1-3)-GlcNAc-phenol
-
-
2.01
Fuc-alpha-(1-4)-GlcNAc-beta-phenol
-
-
1.75
Fuc-alpha-(1-6)-GlcNAc
-
at pH 5.0
0.88
gastric mucin
-
-
-
1.6
glycopeptide from desialyzed porcine submaxillary mucin
Bacillus fulminans
-
-
-
1.02
gp120 oligosaccharide
-
at pH 5.0
-
0.1
lacto-N-fucopentaitol II
-
alpha-fucosidase I
0.095
lacto-N-fucopentaitol III
-
alpha-fucosidase I
2
lacto-N-fucopentaose
Bacillus fulminans
-
-
-
16
methyl alpha-L-fucopyranoside
-
pH 6.5, 37C
0.11 - 1.06
p-nitrophenyl alpha-L-fucopyranoside
0.028 - 2.1
p-nitrophenyl alpha-L-fucoside
0.02 - 0.31
p-nitrophenyl-alpha-L-fucose
0.33
porcine submaxillary mucine, desialyzed
Bacillus fulminans
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-
-