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Literature summary for 3.2.1.51 extracted from
- Identification of an archaeal alpha-L-fucosidase encoded by an interrupted gene. Production of a functional enzyme by mutations mimicking programmed -1 frameshifting (2003), J. Biol. Chem., 278, 14622-14631.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
p-nitrophenyl alpha-L-fucoside | pH 6.3, 65°C | Saccharolobus solfataricus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
10 * 57000, recombinant enzyme | Saccharolobus solfataricus |
| 508000 | - |
gel filtration, recombinant enzyme | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
P2 and MT4 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32.1 | - |
recombinant enzyme | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Two specific mutations, designed on the basis of the programmed -1 frameshifting mechanism, promotes transfucosylation reactions by following a retaining reaction mechanism. | Saccharolobus solfataricus | ? | - |
? | |
| p-nitrophenyl alpha-L-fucoside + H2O | fase II, not: fase I | Saccharolobus solfataricus | p-nitrophenol + alpha-L-fucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | 10 * 57000, recombinant enzyme | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
the activity increases sharply up to the optimal temperature of 95°C, the highest temperature tested | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
high stability | Saccharolobus solfataricus |
| 80 | - |
60% residual activity after 2 h | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 287 | - |
p-nitrophenyl alpha-L-fucoside | pH 6.3, 65°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | 6.3 | 65°C | Saccharolobus solfataricus |
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