Application | Comment | Organism |
---|---|---|
medicine | deficiency in enzyme activity is associated with fucosidosis | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of enzyme is determined at 2.5 A resolution by the MAD method | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
E266A | the mutant exhibit very low hydrolytic activity with p-nitrophenyl alpha-L-fucoside | Thermotoga maritima |
E66A | the mutant retains ability to catalyse the hydrolysis of p-nitrophenyl alpha-L-fucoside and fucosyl fluorid, the Km value is increase dramatically | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
p-nitrophenyl alpha-L-fucoside | pH 5.0, 37°C, wild-type enzyme | Thermotoga maritima | |
0.075 | - |
alpha-L-fucosyl fluoride | pH 5.0, 25°C, mutant E266A | Thermotoga maritima | |
0.15 | - |
alpha-L-fucosyl fluoride | pH 5.0, 25°C, wild-type enzyme | Thermotoga maritima | |
2.1 | - |
p-nitrophenyl alpha-L-fucoside | pH 5.0, 37°C, mutant E66A | Thermotoga maritima | |
2.5 | - |
alpha-L-fucosyl fluoride | pH 5.0, 25°C, mutant E66A | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-fucosyl fluoride + H2O | - |
Thermotoga maritima | alpha-L-fucose + fluoride | - |
? | |
p-nitrophenyl alpha-L-fucoside + H2O | - |
Thermotoga maritima | p-nitrophenol + alpha-L-fucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | hexamer of two trimers | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
alpha-L-fucosyl fluoride | pH 5.0, 25°C, mutant E266A | Thermotoga maritima | |
1.3 | - |
p-nitrophenyl alpha-L-fucoside | pH 5.0, 37°C, mutant E66A | Thermotoga maritima | |
1.4 | - |
alpha-L-fucosyl fluoride | pH 5.0, 25°C, mutant E66A | Thermotoga maritima | |
7.6 | - |
alpha-L-fucosyl fluoride | pH 5.0, 25°C, wild-type enzyme | Thermotoga maritima | |
14.3 | - |
p-nitrophenyl alpha-L-fucoside | pH 5.0, 37°C, wild-type enzyme | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermotoga maritima |