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Literature summary for 3.2.1.51 extracted from
- Glycosidases of molluscs. Purification and properties of alpha-L-fucosidase from Chamelea gallina L. (1976), Eur. J. Biochem., 66, 379-387.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CN- | 30% activation at 10 mM | Chamelea gallina |  |
| ethylenediaminetetraacetic acid | 5% activation at 10 mM | Chamelea gallina | |
General Stability
| General Stability | Organism |
|---|---|
| sensitive to repeated freezing and thawing | Chamelea gallina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-L-fucose | competitive inhibition at 50 mM | Chamelea gallina | |
| D-fucose | 15% inhibition at 50 mM | Chamelea gallina | |
| D-galactono-1,4-lactone | 15% inhibition at 50 mM | Chamelea gallina | |
| D-galactose | 15% inhibition at 50 mM | Chamelea gallina | |
| D-glucono-1,5-lactone | 30% inhibition at 50 mM | Chamelea gallina | |
| D-glucose | 15% inhibition at 50 mM | Chamelea gallina | |
| Hg2+ | effective non-competitive inhibition at 2 mM | Chamelea gallina | |
| L-galactono-1,4-lactone | 30% inhibition at 50 mM | Chamelea gallina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Chamelea gallina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10% activation at 10 mM | Chamelea gallina | |
| Zn2+ | 10% activation at 10 mM | Chamelea gallina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Chamelea gallina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fuc-alpha-(1-2)-Gal + H2O | Chamelea gallina | - |
alpha-L-fucose + D-galactose | - |
? | |
| Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Chamelea gallina | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? | |
| Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | Chamelea gallina | 3-fucosyllactose | alpha-D-fucose + lactose | - |
? | |
| Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Chamelea gallina | lacto-N-fucopentaose III | alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O | Chamelea gallina | - |
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal | - |
? | |
| lacto-N-fucopentaose II + H2O | Chamelea gallina | - |
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| oligosaccharide + H2O | Chamelea gallina | di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds | alpha-L-fucose + ? | - |
? | |
| submaxillary mucin + H2O | Chamelea gallina | - |
alpha-L-fucose + ? | - |
? | |
| thyroglobulin + H2O | Chamelea gallina | - |
alpha-L-fucose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chamelea gallina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, isoelectric focusing | Chamelea gallina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Chamelea gallina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1620 | - |
- |
Chamelea gallina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-L-fucopyranoside + H2O | can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages | Chamelea gallina | 4-nitrophenol + alpha-L-fucose | - |
? | |
| Fuc-alpha-(1-2)-Gal + H2O | - |
Chamelea gallina | alpha-L-fucose + D-galactose | - |
? | |
| Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Chamelea gallina | alpha-L-fucose + lactose | - |
? | |
| Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | 3-fucosyllactose | Chamelea gallina | alpha-L-fucose + lactose | - |
? | |
| Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O | 3-fucosyllactose | Chamelea gallina | alpha-D-fucose + lactose | - |
? | |
| Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaose III | Chamelea gallina | alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O | - |
Chamelea gallina | alpha-L-fucose + GalNAc-alpha-(1-3)-Gal | - |
? | |
| lacto-N-fucopentaose II + H2O | - |
Chamelea gallina | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| oligosaccharide + H2O | di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds | Chamelea gallina | alpha-L-fucose + ? | - |
? | |
| oligosaccharides + H2O | di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds | Chamelea gallina | alpha-L-fucose + ? | - |
? | |
| submaxillary mucin + H2O | - |
Chamelea gallina | alpha-L-fucose + ? | - |
? | |
| thyroglobulin + H2O | - |
Chamelea gallina | alpha-L-fucose + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
fully active after 5 min | Chamelea gallina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Chamelea gallina |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | stable between | Chamelea gallina |
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