KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
4-nitrophenyl-alpha-L-fucopyranoside | sperm-bound enzyme | Unio elongatulus | |
0.91 | - |
4-nitrophenyl-alpha-L-fucopyranoside | gonad fluid enzyme | Unio elongatulus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Unio elongatulus | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55970 | - |
gonad fluid enzyme, mass spectrometry | Unio elongatulus |
55971 | - |
1 * 55971, gonad fluid enzyme, 1 * 67847, sperm-bound enzyme, mass spectrometry | Unio elongatulus |
67847 | - |
1 * 55971, gonad fluid enzyme, 1 * 67847, sperm-bound enzyme, mass spectrometry | Unio elongatulus |
67850 | - |
sperm-bound enzyme, mass spectrometry | Unio elongatulus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Unio elongatulus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-linked oligosaccharides only for the gonad fluid enzyme, sperm-bound enzyme not N- or O-linked glycosylated | Unio elongatulus |
Purification (Comment) | Organism |
---|---|
to homogeneity, two forms, affinity chromatography | Unio elongatulus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gonad | fluid | Unio elongatulus | - |
semen | one form tightly bound to the sperm plasma membrane, one form present in the gonad fluid | Unio elongatulus | - |
Storage Stability | Organism |
---|---|
4°C, 10 mM sodium phosphate, pH 5.5, 1 mM EDTA, 1 mM PMSF, one month | Unio elongatulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Unio elongatulus | 4-nitrophenol + alpha-L-fucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55971, gonad fluid enzyme, 1 * 67847, sperm-bound enzyme, mass spectrometry | Unio elongatulus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Unio elongatulus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
gonad fluid enzyme sharper pH dependence curve than the sperm-bound enzyme | Unio elongatulus |