Literature summary for 3.2.1.51 extracted from

Merino-Trigo, A.; Rodriguez-Berrocal, F.J.; de Miguel, E.; Paez de la Cadena, M.
Activity and properties of alpha-L-fucosidase are dependent on the state of enterocytic differentiation of HT-29 colon cancer cells (2002), Int. J. Biochem. Cell Biol., 34, 1291-1303.

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Application

Application	Comment	Organism
medicine	the differentiation of HT-29 colon cancer cells can be used as a model to study the alteration of the enzyme alpha-L-fucosidase during the progression of the tumoural processs	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06	-	4-methylumbelliferyl alpha-L-fucopyranoside	pH 5.0, 37°C, undifferentiated HT-29 cells	Homo sapiens
0.07	-	4-methylumbelliferyl alpha-L-fucopyranoside	pH 5.0, 37°C, differentiated HT-29 cells	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HT-29 cell	cell colorectal cancer cells	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	variation of activity during differentiation	Homo sapiens
0.003	-	non-differentiated HT-29 cells	Homo sapiens
0.009	-	differentiated HT-29 cells	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-L-fucopyranoside + H2O	-	Homo sapiens	4-methylumbelliferone + alpha-L-fucose	-	?

Subunits

Subunits	Comment	Organism
More	comparison of elution profile of enzyme from undifferentiated and differentiated HT-29 cells through gel filtration and SDS-PAGE, presence of a 50000 Da protein in both cultures	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	comparison of heat stability of enzyme from undifferentiated and differentiated HT-29 cells	Homo sapiens

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	comparison of pH stability of enzyme from undifferentiated and differentiated HT-29 cells, enzymes from undifferentiated and differentiated HT-29 cells are very stable at neutral and basic pH values	Homo sapiens

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Release 2023.1 (January 2023)