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Literature summary for 3.2.1.51 extracted from
- Recombinant canine alpha-L-fucosidase: Expression, purification, and characterization (2000), Mol. Genet. Metab., 69, 24-32.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | recombinant enzyme will extend the proven efficiacy of enzyme replacement therapy in lysosomal storage disorders e.g. fucosidosis | Canis lupus lupus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in CHO cells | Canis lupus lupus |
| expression in MDCK cells | Canis lupus lupus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | - |
Canis lupus lupus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Canis lupus lupus |
| 156000 | - |
gel filtration | Canis lupus lupus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus lupus | - |
canine | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 10%, w/w, carbohydrate content | Canis lupus lupus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, recombinant enzyme | Canis lupus lupus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.9 | 17.1 | recombinant enzyme, value depending on expression systems and buffer | Canis lupus lupus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Canis lupus lupus | 4-methylumbelliferone + alpha-L-fucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Canis lupus lupus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
fluorogenic substrate, natural substrates, isolated from dog brain, more readily digested at acidic pH | Canis lupus lupus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Canis lupus lupus |
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