Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-L-fucose | competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis | Thermus sp. | |
fucose | - |
Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
p-nitrophenyl alpha-L-fucopyranoside | pH 6.5, 37°C | Thermus sp. | |
3 | - |
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc | pH 6.5, 37°C | Thermus sp. | |
7 | - |
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me | pH 6.5, 37°C | Thermus sp. | |
9 | - |
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc | pH 6.5, 37°C | Thermus sp. | |
16 | - |
methyl alpha-L-fucopyranoside | pH 6.5, 37°C | Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
4 * 61000, SDS-PAGE | Thermus sp. |
240000 | - |
gel filtration | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Thermus sp. Y5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an alpha-L-fucoside + H2O = L-fucose + an alcohol | the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides | Thermus sp. |
Storage Stability | Organism |
---|---|
4°C, enzyme freeze dried from aqueous solution containing 3 mM DTT and 5-20 mg/ml bovine serum albumin retains full activity for at least one year | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O | - |
Thermus sp. | alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc | - |
? | |
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O | - |
Thermus sp. Y5 | alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc | - |
? | |
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O | - |
Thermus sp. | alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me | - |
? | |
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O | - |
Thermus sp. Y5 | alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me | - |
? | |
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O | - |
Thermus sp. | alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc | - |
? | |
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O | - |
Thermus sp. Y5 | alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc | - |
? | |
methyl alpha-L-fucopyranoside + H2O | or methanol or ethanol as acceptor | Thermus sp. | methanol + alpha-L-fucose | - |
? | |
methyl alpha-L-fucopyranoside + H2O | or methanol or ethanol as acceptor | Thermus sp. Y5 | methanol + alpha-L-fucose | - |
? | |
additional information | not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside | Thermus sp. | ? | - |
? | |
additional information | not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside | Thermus sp. Y5 | ? | - |
? | |
p-nitrophenyl alpha-L-fucopyranoside + H2O | or methanol or ethanol as acceptor | Thermus sp. | p-nitrophenol + alpha-L-fucopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 61000, SDS-PAGE | Thermus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
optimal temperatures for hydrolysis | Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the enzyme is stable overnight in the pH range from 5.5 to 7.5 | Thermus sp. |
45 | - |
thermal inactivation at temperatures above 45°C | Thermus sp. |
60 | - |
the activity decreases rapidly at temperatures above 60°C | Thermus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | the enzyme is stable overnight at 37°C | Thermus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
fucose | pH 6.5, 37°C | Thermus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermus sp. | - |
- |
4.2 |