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Literature summary for 3.2.1.51 extracted from

  • Eneyskaya, E.V.; Kulminskaya, A.A.; Kalkkinen, N.; Nifantiev, N.E.; Arbatskii, N.P.; Saenko, A.I.; Chepurnaya, O.V.; Arutyunyan, A.V.; Shabalin, K.A.; Neustroev, K.N.
    An alpha-L-fucosidase from Thermus sp. with unusually broad specificity (2001), Glycoconj. J., 18, 827-834.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alpha-L-fucose competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis Thermus sp.
fucose
-
Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
p-nitrophenyl alpha-L-fucopyranoside pH 6.5, 37°C Thermus sp.
3
-
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc pH 6.5, 37°C Thermus sp.
7
-
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me pH 6.5, 37°C Thermus sp.
9
-
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc pH 6.5, 37°C Thermus sp.
16
-
methyl alpha-L-fucopyranoside pH 6.5, 37°C Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
4 * 61000, SDS-PAGE Thermus sp.
240000
-
gel filtration Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-
Thermus sp. Y5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus sp.

Reaction

Reaction Comment Organism Reaction ID
an alpha-L-fucoside + H2O = L-fucose + an alcohol the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides Thermus sp.

Storage Stability

Storage Stability Organism
4°C, enzyme freeze dried from aqueous solution containing 3 mM DTT and 5-20 mg/ml bovine serum albumin retains full activity for at least one year Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
Thermus sp. alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
Thermus sp. Y5 alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
Thermus sp. alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
Thermus sp. Y5 alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
Thermus sp. alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
Thermus sp. Y5 alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
?
methyl alpha-L-fucopyranoside + H2O or methanol or ethanol as acceptor Thermus sp. methanol + alpha-L-fucose
-
?
methyl alpha-L-fucopyranoside + H2O or methanol or ethanol as acceptor Thermus sp. Y5 methanol + alpha-L-fucose
-
?
additional information not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside Thermus sp. ?
-
?
additional information not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside Thermus sp. Y5 ?
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O or methanol or ethanol as acceptor Thermus sp. p-nitrophenol + alpha-L-fucopyranose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 61000, SDS-PAGE Thermus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
optimal temperatures for hydrolysis Thermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the enzyme is stable overnight in the pH range from 5.5 to 7.5 Thermus sp.
45
-
thermal inactivation at temperatures above 45°C Thermus sp.
60
-
the activity decreases rapidly at temperatures above 60°C Thermus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 the enzyme is stable overnight at 37°C Thermus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.15
-
fucose pH 6.5, 37°C Thermus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Thermus sp.
-
-
4.2