Information on EC 3.1.1.20 - tannase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.1.1.20
-
RECOMMENDED NAME
GeneOntology No.
tannase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
digallate + H2O = 2 gallate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
-
hydrolysis of carboxylic ester
-
the enzyme also shows beta-glucosidase activity
hydrolysis of carboxylic ester
Aspergillus aculeatus DBF 9, Bacillus licheniformis KBR 6, Aspergillus niger MTCC 2425
-
-
-
SYSTEMATIC NAME
IUBMB Comments
tannin acylhydrolase
Also hydrolyses ester links in other tannins.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
depsidase
-
-
fungal tannase
-
-
fungal tannase
Aspergillus niger Aa-20
-
-
-
gallotannin-degrading esterase
-
-
GALLO_1609
DEHEY7
locus name
GALLO_1609
DEHEY7
locus name
-
plant tannase
-
-
TAH I
-
isoform
TAH I
Verticillium sp. P9
-
isoform
-
TAH II
-
isoform
TAH II
Verticillium sp. P9
-
isoform
-
Tan410
E7D7J5
-
TanA
Lactobacillus plantarum ATCC 14917T
-
-
-
tannase
Aspergillus awamori MTCC9299
-
-
-
tannase
Aspergillus niger GH1
-
-
-
tannase
Aspergillus tamarii IMI388810 (B)
-
-
-
tannase
Bacillus licheniformis KBR6
-
-
-
tannase
Hyalopus sp.
-
-
tannase
Hyalopus sp. DSF3
-
-
-
tannase
Lactobacillus plantarum CECT 748T
-
-
-
tannase I
-
degrades ester-containing polygallol derivatives
tannase II
-
hydrolzes polygallol derivatives containing depside groups
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Aspergillus aculeatus DBF 9
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
C7F6Y1
-
tannin acyl hydrolase
Aspergillus awamori BTMFW032
-, C7F6Y1
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Aspergillus foetidus MTCC 6322
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Aspergillus heteromorphus MTCC 8818
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Aspergillus niger GH1, Aspergillus niger MTCC 2425
-
-
-
tannin acyl hydrolase
Aspergillus oryzae No. 7
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Bacillus licheniformis KBR 6, Bacillus licheniformis KBR6
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
C7EDT0
-
tannin acyl hydrolase
Enterobacter sp. KPJ103
C7EDT0
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Lactobacillus paraplantarum NSO120
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Lactobacillus pentosus 21A-3
-
-
-
tannin acyl hydrolase
B3Y018
-
tannin acyl hydrolase
B3Y018
tannase
tannin acyl hydrolase
Lactobacillus plantarum ATCC 14917T
-, B3Y018
-
-
tannin acyl hydrolase
Lactobacillus plantarum CECT 748T, Lactobacillus plantarum CIR1
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Penicillium chrysogenum NCIM 923
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Penicillium sp. EZ-ZH190, Penicillium sp. EZ-ZH290, Penicillium sp. EZ-ZH390
-
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Rhizopus sp.
-
-
tannin acyl hydrolase
Rhizopus sp. C3-I
-
-
-
tannin acyl hydrolase
DEHEY7
-
tannin acyl hydrolase
DEHEY7
-
-
tannin acyl hydrolase
-
belongs to the class of serine hydrolases
tannin acyl hydrolase
Talaromyces variabilis IARI 2031
-
belongs to the class of serine hydrolases
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
Trichoderma sp.
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
-
-
tannin acyl hydrolase
E7D7J5
-
tannin acyl hydrolase
-
-
tannin acyl-hydrolase
-
-
tannin acyl-hydrolase
Corynebacterium sp. Q40
-
-
-
tannin acyl-hydrolase
-
-
tannin acyl-hydrolase
Klebsiella pneumoniae Q52
-
-
-
tannin acyl-hydrolase
-
-
tannin acyl-hydrolase
Paenibacillus polymyxa Q47
-
-
-
tannin acylhydrolase
-
-
tannin acylhydrolase
Aspergillus aculeatus DBF 9
-
-
-
tannin acylhydrolase
Aspergillus niger ATTC 16620, Aspergillus niger GH1, Aspergillus niger ITCC 6514.07, Aspergillus niger van Tieghem
-
-
-
tannin acylhydrolase
Bacillus cereus KBR9
-
-
-
tannin acylhydrolase
-
-
tannin acylhydrolase
Bacillus licheniformis KBR 6
-
-
-
tannin acylhydrolase
B3Y018
-
tannin acylhydrolase
Lactobacillus plantarum ATCC 1491 T
-
-
-
tannin acylhydrolase
Lactobacillus plantarum SN35N
B3Y018
-
-
tannin acylhydrolase
-
-
tannin acylhydrolase
Verticillium sp. P9
-
-
-
tannin-acyl-hydrolase
-
-
tannin-acyl-hydrolase
Bacillus licheniformis KBR6
-
-
-
yeast tannase
Candida sp.
-
-
yeast tannase
Candida sp. K-1
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9025-71-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain DBF 9, intra-and extracellular enzyme forms, inducible, fungus can utilize tannic acid as sole carbon and energy source
-
-
Manually annotated by BRENDA team
strain DBF9
-
-
Manually annotated by BRENDA team
Aspergillus aculeatus DBF 9
-
-
-
Manually annotated by BRENDA team
Aspergillus aculeatus DBF 9
strain DBF 9, intra-and extracellular enzyme forms, inducible, fungus can utilize tannic acid as sole carbon and energy source
-
-
Manually annotated by BRENDA team
Aspergillus aculeatus DBF9
strain DBF9
-
-
Manually annotated by BRENDA team
subsp. nakazawa
-
-
Manually annotated by BRENDA team
var. Nakazawa
-
-
Manually annotated by BRENDA team
Aspergillus awamori BTMFW032
-
-
-
Manually annotated by BRENDA team
Aspergillus awamori BTMFW032
-
UniProt
Manually annotated by BRENDA team
Aspergillus awamori MTCC9299
MTCC9299
-
-
Manually annotated by BRENDA team
Aspergillus ficuum Gim 3.6
-
-
-
Manually annotated by BRENDA team
IFO 5839
-
-
Manually annotated by BRENDA team
isolated from a composite tannery effluent collected from a local tannery
-
-
Manually annotated by BRENDA team
GMRB013 MTCC 3557
-
-
Manually annotated by BRENDA team
strain GMRB 013 MTCC 3557
-
-
Manually annotated by BRENDA team
strain MTCC 6322
-
-
Manually annotated by BRENDA team
Aspergillus foetidus GMRB 013 MTCC 3557
strain GMRB 013 MTCC 3557
-
-
Manually annotated by BRENDA team
Aspergillus foetidus MTCC 6322
strain MTCC 6322
-
-
Manually annotated by BRENDA team
Aspergillus heteromorphus MTCC 8818
-
-
-
Manually annotated by BRENDA team
synonym Aspergillus nidulans
-
-
Manually annotated by BRENDA team
inducible enzyme
-
-
Manually annotated by BRENDA team
IRDUAM collection strain No. Aa20
-
-
Manually annotated by BRENDA team
PKL 104
-
-
Manually annotated by BRENDA team
strain Aa-20
-
-
Manually annotated by BRENDA team
strain ATCC 16620
-
-
Manually annotated by BRENDA team
strain GH1
-
-
Manually annotated by BRENDA team
strain HA37
-
-
Manually annotated by BRENDA team
strain MTCC 2425
-
-
Manually annotated by BRENDA team
strain MTCC 2425, and a strain isolated from a composite tannery effluent collected from a local tannery
-
-
Manually annotated by BRENDA team
strain van Tieghem MTCC 2425
-
-
Manually annotated by BRENDA team
Aspergillus niger Aa-20
strain Aa-20
-
-
Manually annotated by BRENDA team
Aspergillus niger ATTC 16620
-
-
-
Manually annotated by BRENDA team
Aspergillus niger GH1
-
-
-
Manually annotated by BRENDA team
Aspergillus niger GH1
strain GH1
-
-
Manually annotated by BRENDA team
Aspergillus niger HA37
strain HA37
-
-
Manually annotated by BRENDA team
Aspergillus niger ITCC 6514.07
-
-
-
Manually annotated by BRENDA team
Aspergillus niger LCF 8
LCF 8
-
-
Manually annotated by BRENDA team
Aspergillus niger MTCC 2425
strain MTCC 2425
-
-
Manually annotated by BRENDA team
Aspergillus niger PKL 104
PKL 104
-
-
Manually annotated by BRENDA team
Aspergillus niger T3-5-1
-
-
-
Manually annotated by BRENDA team
Aspergillus niger van Tieghem
-
-
-
Manually annotated by BRENDA team
2 isozymes
-
-
Manually annotated by BRENDA team
expression in Pichia pastoris
-
-
Manually annotated by BRENDA team
Aspergillus oryzae No. 7
No. 7
-
-
Manually annotated by BRENDA team
strain SHL 6, induction of enzyme by growth on peptone
-
-
Manually annotated by BRENDA team
Aspergillus sp. SHL 6
strain SHL 6, induction of enzyme by growth on peptone
-
-
Manually annotated by BRENDA team
IMI388810 (B)
-
-
Manually annotated by BRENDA team
Aspergillus tamarii IMI388810 (B)
IMI388810 (B)
-
-
Manually annotated by BRENDA team
strain DBS66, isolated from forest soil in india
-
-
Manually annotated by BRENDA team
Aureobasidium pullulans DBS66
strain DBS66, isolated from forest soil in india
-
-
Manually annotated by BRENDA team
KBR 9, inducible enzyme
-
-
Manually annotated by BRENDA team
Bacillus cereus KBR9
-
-
-
Manually annotated by BRENDA team
strain KBR 6
-
-
Manually annotated by BRENDA team
strain KBR 6 isolated from lateritic soil, inducible enzyme
-
-
Manually annotated by BRENDA team
strain KBR6
-
-
Manually annotated by BRENDA team
strain KBR6, inducible enzyme
-
-
Manually annotated by BRENDA team
Bacillus licheniformis KBR 6
-
-
-
Manually annotated by BRENDA team
Bacillus licheniformis KBR 6
strain KBR 6
-
-
Manually annotated by BRENDA team
Bacillus licheniformis KBR 6
strain KBR 6 isolated from lateritic soil, inducible enzyme
-
-
Manually annotated by BRENDA team
Bacillus licheniformis KBR6
strain KBR6
-
-
Manually annotated by BRENDA team
Bacillus licheniformis KBR6
strain KBR6, inducible enzyme
-
-
Manually annotated by BRENDA team
synonym Blastobotrys adeninivorans
-
-
Manually annotated by BRENDA team
Blastobotrys adeninivorans LS3
strain LS3
Uniprot
Manually annotated by BRENDA team
Candida sp.
-
-
-
Manually annotated by BRENDA team
Candida sp.
K-1
-
-
Manually annotated by BRENDA team
Candida sp. K-1
K-1
-
-
Manually annotated by BRENDA team
Corynebacterium sp. Q40
Q40
-
-
Manually annotated by BRENDA team
Enterobacter sp. KPJ103
-
UniProt
Manually annotated by BRENDA team
isolated from a composite tannery effluent collected from a local tannery
-
-
Manually annotated by BRENDA team
Hyalopus sp.
-
-
-
Manually annotated by BRENDA team
Hyalopus sp.
strain DSF3
-
-
Manually annotated by BRENDA team
Hyalopus sp. DSF3
strain DSF3
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae Q52
Q52
-
-
Manually annotated by BRENDA team
Lactobacillus paraplantarum NSO120
-
-
-
Manually annotated by BRENDA team
Lactobacillus pentosus 21A-3
-
-
-
Manually annotated by BRENDA team
CECT 748T strain
-
-
Manually annotated by BRENDA team
inducible enzyme
-
-
Manually annotated by BRENDA team
strain ATCC 14917(T)
SwissProt
Manually annotated by BRENDA team
strain CECT 748(T), ATCC 14917, DSMZ 20174
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum ATCC 1491 T
-
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum ATCC 14917T
-
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum ATCC 14917T
-
SwissProt
Manually annotated by BRENDA team
Lactobacillus plantarum CECT 748(T)
strain CECT 748(T), ATCC 14917, DSMZ 20174
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum CECT 748T
-
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum CECT 748T
CECT 748T strain
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum CIR1
-
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum SN35N
-
SwissProt
Manually annotated by BRENDA team
strain ASR-S1, induction of enzyme by solid-state fermentation on tamarind seed powder, wheat bran, or coffee husk
-
-
Manually annotated by BRENDA team
Lactobacillus sp. ASR-S1
strain ASR-S1, induction of enzyme by solid-state fermentation on tamarind seed powder, wheat bran, or coffee husk
-
-
Manually annotated by BRENDA team
i.e. Divi-Divi
-
-
Manually annotated by BRENDA team
a strain isolated in Sao Paulo, Brazil
-
-
Manually annotated by BRENDA team
Paenibacillus polymyxa Q47
Q47
-
-
Manually annotated by BRENDA team
Penicillium chrysogenum NCIM 923
-
-
-
Manually annotated by BRENDA team
Penicillium chrysogenum NCIM-722
NCIM-722
-
-
Manually annotated by BRENDA team
isolated from a composite tannery effluent collected from a local tannery
-
-
Manually annotated by BRENDA team
Penicillium sp. EZ-ZH190
-
-
-
Manually annotated by BRENDA team
Penicillium sp. EZ-ZH290
-
-
-
Manually annotated by BRENDA team
Penicillium sp. EZ-ZH390
-
-
-
Manually annotated by BRENDA team
Qercus pedunculata
-
-
Manually annotated by BRENDA team
RO IIT RB-13, NRRL 21498
-
-
Manually annotated by BRENDA team
strain RO IIT RB-13, NRRL 21498
-
-
Manually annotated by BRENDA team
Rhizopus oryzae RO IIT RB-13
strain RO IIT RB-13, NRRL 21498
-
-
Manually annotated by BRENDA team
Rhizopus sp.
strain C3-I
-
-
Manually annotated by BRENDA team
Rhizopus sp. C3-I
strain C3-I
-
-
Manually annotated by BRENDA team
subsp. ruminantium
-
-
Manually annotated by BRENDA team
strain IARI 2031
-
-
Manually annotated by BRENDA team
Talaromyces variabilis IARI 2031
strain IARI 2031
-
-
Manually annotated by BRENDA team
Trichoderma sp.
isolated from a composite tannery effluent collected from a local tannery
-
-
Manually annotated by BRENDA team
Antarctic strain P9, two cold-adapted isozymes TAH I and TAH II
-
-
Manually annotated by BRENDA team
Verticillium sp. P9
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
B7VFD0, -
enzyme titre with the recombinant strain (390 U/l) is approximately 10times higher than that in the control strain without the TEF1 promoter-ATAN1 gene expression cassette. The Atan1 protein contains at positions 210-214 the canonical Gly-X-Ser-X-Gly motif found in the serine hydrolases as the catalytic triad for nucleophilic serine
physiological function
-
tanA gene is specific to Staphylococcus lugdunensis
physiological function
-
the enzyme production follows logarithmic growth phase with maximum enzyme yield being obtained after 6 days corresponding to the culture pH of 3.8
physiological function
C7EDT0
tannase and the organism itself are employed to protect grazing animals and environment against the toxic effects caused by tannins in them
physiological function
Aspergillus tamarii IMI388810 (B)
-
the enzyme production follows logarithmic growth phase with maximum enzyme yield being obtained after 6 days corresponding to the culture pH of 3.8
-
physiological function
Enterobacter sp. KPJ103
-
tannase and the organism itself are employed to protect grazing animals and environment against the toxic effects caused by tannins in them
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+/-)-epicatechin-gallate + H2O
epicatechin + gallate
show the reaction diagram
-
-
-
-
?
(+/-)-epigallocatechin-3-gallate + H2O
epigallocatechin + gallate
show the reaction diagram
-
-
-
-
?
(-)-epicatechin gallate + H2O
(-)-epicatechin + gallic acid
show the reaction diagram
-
from green tea leaves
-
-
?
(-)-epigallocatechin gallate + H2O
(-)-epigallocatechin + gallic acid
show the reaction diagram
-
from green tea leaves
-
-
?
1,2,3,4,6-pentagalloyl glucose + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
-
1,2,3,4,6-pentagalloyl glucose + H2O
gallic acid + D-glucose
show the reaction diagram
-
89% activity compared to methyl gallate
-
-
?
1,2,3,6-tetra-O-galloyl-beta-D-glucose + H2O
?
show the reaction diagram
-
-
-
-
?
1,2,6-tri-O-galloyl-beta-D-glucose + H2O
?
show the reaction diagram
-
-
-
-
?
1,6-di-O-galloyl-beta-D-glucose + H2O
?
show the reaction diagram
-
-
-
-
?
1-O-anisoyl-beta-D-glucose + H2O
anisic acid + glucose
show the reaction diagram
-
-
-
-
?
1-O-benzoyl-beta-D-glucose + H2O
benzoic acid + beta-D-glucose
show the reaction diagram
-
-
-
-
?
1-O-galloyl-beta-D-glucose + H2O
gallic acid + beta-D-glucose
show the reaction diagram
-
-
-
-
-
1-O-galloyl-beta-D-glucose + H2O
gallic acid + beta-D-glucose
show the reaction diagram
-
-
-
-
?
1-O-p-hydroxybenzoyl-beta-D-glucose + H2O
4-hydroxybenzoate + beta-D-glucose
show the reaction diagram
-
-
-
-
?
1-O-protocatechuoyl-beta-D-glucose + H2O
protocatechuate + glucose
show the reaction diagram
-
-
-
-
?
1-O-syringoyl-beta-D-glucose + H2O
4-hydroxy-3,5-dimethoxy benzoic acid + beta-D-glucose
show the reaction diagram
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + H2O
4-hydroxy-3-methoxy benzoic acid + beta-D-glucose
show the reaction diagram
-
-
-
-
?
1-O-veratroyl-beta-D-glucose + H2O
3,4-dimethyoxy benzoic acid + beta-D-glucose
show the reaction diagram
-
-
-
-
?
1-propanol + gallic acid
propyl gallate + H2O
show the reaction diagram
-
the reaction takes place in organic solvents, best in benzene, effect of water content, overview
-
-
r
3,6-di-O-galloyl-beta-D-glucose + H2O
?
show the reaction diagram
-
-
-
-
?
4-O-digalloyl-1,2,3,6-tetra-O-galloyl-beta-D-glucose + H2O
?
show the reaction diagram
-
-
-
-
?
6-O-galloyl-beta-D-glucose + H2O
gallic acid + beta-D-glucose
show the reaction diagram
-
-
-
-
?
butyl 4-hydroxybenzoate + H2O
butanol + 4-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
butyl 4-hydroxybenzoate + H2O
butanol + 4-hydroxybenzoate
show the reaction diagram
DEHEY7
-
-
-
?
catechin gallate + H2O
gallic acid + catechin
show the reaction diagram
-
-
-
?
catechin gallate + H2O
gallic acid + catechin
show the reaction diagram
B7VFD0, -
best substrate
-
-
?
catechin gallate + H2O
?
show the reaction diagram
Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus pentosus 21A-3, Lactobacillus paraplantarum NSO120
-
-
-
-
?
catechin gallate + H2O
?
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
B3Y018
-
-
-
?
catechin gallate + H2O
gallate + catechin
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
-
beta-glucosidase activity of the enzyme, only observed in absence of tannic acid
-
?
chlorogenic acid + H2O
?
show the reaction diagram
-
-
-
-
?
chlorogenic acid + H2O
trans-caffeate + quinate
show the reaction diagram
E7D7J5
complete hydrolysis within 40 min
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
B3Y018, -
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
substrate: tannins
-
?
digallate + H2O
gallate
show the reaction diagram
-
substrate: tannins
i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
-
substrate: tannins
i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
Verticillium sp. P9, Aspergillus aculeatus DBF 9
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Aspergillus aculeatus DBF 9
-
substrate: tannins
-, i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
Aspergillus niger ATTC 16620, Aspergillus niger GH1, Bacillus licheniformis KBR 6
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Bacillus licheniformis KBR 6
-
substrate: tannins
i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
Aspergillus niger van Tieghem, Lactobacillus plantarum ATCC 1491 T, Aspergillus niger ITCC 6514.07
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Penicillium chrysogenum NCIM 923
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Bacillus cereus KBR9
-
-
-
-
?
ellagitannin + H2O
hexahydroxydiphenic acid + ?
show the reaction diagram
-
-
-
-
?
ellagitannins + H2O
hexahydroxydiphenic acid + gallic acid
show the reaction diagram
Aspergillus niger, Libidibia coriaria, Aspergillus niger Aa-20
-
-
-
-
?
epicatechin gallate + H2O
gallic acid + epicatechin
show the reaction diagram
-
-
-
?
epicatechin gallate + H2O
gallic acid + epicatechin
show the reaction diagram
-
-
-
-
?
epicatechin gallate + H2O
gallic acid + epicatechin
show the reaction diagram
B7VFD0, -
-
-
-
?
epicatechin gallate + H2O
gallate + epicatechin
show the reaction diagram
-
best substrate
-
-
?
epicatechin gallate + H2O
gallate + epicatechin
show the reaction diagram
E7D7J5
complete hydrolysis within 40 min
-
-
?
epicatechin gallate + H2O
?
show the reaction diagram
Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus pentosus 21A-3, Lactobacillus paraplantarum NSO120
-
-
-
-
?
epicatechin gallate + H2O
?
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
B3Y018
-
-
-
?
epigallocatechin gallate + H2O
gallic acid + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallic acid + epigallocatechin
show the reaction diagram
-
-
-
?
epigallocatechin gallate + H2O
gallic acid + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallic acid + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallic acid + epigallocatechin
show the reaction diagram
B7VFD0, -
-
-
-
?
epigallocatechin gallate + H2O
gallic acid + epigallocatechin
show the reaction diagram
Lactobacillus plantarum CECT 748T
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Trametes elegans, Hyalopus sp.
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
E7D7J5
complete hydrolysis within 40 min
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Verticillium sp. P9, Aspergillus aculeatus DBF 9, Aspergillus niger ATTC 16620, Aspergillus niger GH1
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Lactobacillus pentosus 21A-3
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Lactobacillus paraplantarum NSO120
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Aspergillus niger van Tieghem
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
B3Y018
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Lactobacillus plantarum ATCC 1491 T, Aspergillus niger ITCC 6514.07
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Penicillium chrysogenum NCIM 923
-
-
-
-
?
epigallocatechin gallate + H2O
gallate + epigallocatechin
show the reaction diagram
Bacillus cereus KBR9
-
-
-
-
?
epigallocatechin gallate + H2O
epigalocatechin + gallate
show the reaction diagram
-
-
-
-
?
epigallocatechin gallate + H2O
epigalocatechin + gallate
show the reaction diagram
DEHEY7
-
-
-
?
epigallocatechin-3-O-(3-O-methyl) gallate + H2O
?
show the reaction diagram
-
-
-
-
?
ethyl 3,4-dihydroxybenzoate + H2O
mthanol + 3,4-dihydroxybenzoate
show the reaction diagram
-
-
-
-
?
ethyl 3,4-dihydroxybenzoate + H2O
mthanol + 3,4-dihydroxybenzoate
show the reaction diagram
DEHEY7
-
-
-
?
ethyl 3,5-dihydroxybenzoate + H2O
ethanol + 3,5-dihydroxybenzoate
show the reaction diagram
-
-
-
-
?
ethyl 3,5-dihydroxybenzoate + H2O
ethanol + 3,5-dihydroxybenzoate
show the reaction diagram
DEHEY7
-
-
-
?
ethyl 4-hydroxybenzoate + H2O
ethanol + 4-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
ethyl 4-hydroxybenzoate + H2O
ethanol + 4-hydroxybenzoate
show the reaction diagram
DEHEY7
-
-
-
?
ethyl 4-hydroxybenzoate + H2O
ethanol + 4-hydroxybenzoate
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
-
-
-
-
?
ethyl 4-hydroxybenzoate + H2O
ethanol + 4-hydroxybenzoate
show the reaction diagram
DEHEY7, -
-
-
-
?
ethyl benzoate + H2O
ethanol + benzoate
show the reaction diagram
-
-
-
-
?
ethyl benzoate + H2O
ethanol + benzoate
show the reaction diagram
DEHEY7
-
-
-
?
ethyl benzoate + H2O
ethanol + benzoate
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
-
-
-
-
?
ethyl benzoate + H2O
ethanol + benzoate
show the reaction diagram
DEHEY7, -
-
-
-
?
ethyl ferulate + H2O
ethanol + ferulate
show the reaction diagram
E7D7J5
complete hydrolysis within 40 min
-
-
?
ethyl gallate + H2O
gallic acid + ethanol
show the reaction diagram
-
-
-
-
?
ethyl gallate + H2O
gallic acid + ethanol
show the reaction diagram
-
-
-
-
?
ethyl gallate + H2O
gallic acid + ethanol
show the reaction diagram
Aspergillus oryzae, Aspergillus oryzae No. 7
-
55% activity compared to methyl gallate
-
-
?
ethyl gallate + H2O
gallic acid + ethanol
show the reaction diagram
Lactobacillus plantarum CECT 748T
-
-
-
-
?
ethyl gallate + H2O
gallate + ethanol
show the reaction diagram
-
-
-
-
?
ethyl gallate + H2O
gallate + ethanol
show the reaction diagram
DEHEY7
-
-
-
?
ethyl gallate + H2O
gallate + ethanol
show the reaction diagram
-
worst substrate
-
-
?
ferulic ethyl ester + H2O
ethanol + ferulate
show the reaction diagram
-
-
-
-
?
ferulic ethyl ester + H2O
ethanol + ferulate
show the reaction diagram
DEHEY7
-
-
-
?
ferulic methyl ester + H2O
methanol + ferulate
show the reaction diagram
-
-
-
-
?
ferulic methyl ester + H2O
methanol + ferulate
show the reaction diagram
DEHEY7
-
-
-
?
gallate + 1-butanol
1-butyl gallate + H2O
show the reaction diagram
-
85% of the yield with 1-propanol
-
-
r
gallate + 1-hexanol
1-hexyl gallate + H2O
show the reaction diagram
-
2% of the yield with 1-propanol
-
-
r
gallate + 1-pentanol
1-pentyl gallate + H2O
show the reaction diagram
-
77% of the yield with 1-propanol
-
-
r
gallate + ethanol
ethyl gallate + H2O
show the reaction diagram
-
5% of the yield with 1-propanol
-
-
r
gallate + methanol
methyl gallate + H2O
show the reaction diagram
-
2% of the yield with 1-propanol
-
-
r
gallate + propan-1-ol
propyl gallate + H2O
show the reaction diagram
-
-
-
-
r
gallic acid ethyl ester + H2O
gallic acid + ethanol
show the reaction diagram
B7VFD0, -
-
-
-
?
gallic acid methyl ester + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
?
gallic acid propyl ester + H2O
gallic acid + propanol
show the reaction diagram
B7VFD0, -
-
-
-
?
gallocatechin gallate + H2O
gallic acid + gallocatechin
show the reaction diagram
-
-
-
-
?
gallocatechin gallate + H2O
gallic acid + gallocatechin
show the reaction diagram
B7VFD0, -
-
-
-
?
gallocatechin gallate + H2O
gallic acid + gallocatechin
show the reaction diagram
-
best substrate
-
?
gallocatechin gallate + H2O
gallic acid + gallocatechin
show the reaction diagram
Lactobacillus plantarum CECT 748T
-
-
-
-
?
gallotanin + H2O
gallate + D-glucose
show the reaction diagram
DEHEY7
-
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
Rhizopus sp.
-
-
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
-
hydrolysis of ester and depside linkages of gallotannins via galloylglucose
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
Aspergillus niger Aa-20
-
-, hydrolysis of ester and depside linkages of gallotannins via galloylglucose
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
Bacillus licheniformis KBR 6
-
-, hydrolysis of ester and depside linkages of gallotannins via galloylglucose
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
Rhizopus sp. C3-I
-
-
-
-
?
gallotannin + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
gallotannin + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
gallotannin + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
gallotannin + H2O
gallate + D-glucose
show the reaction diagram
Verticillium sp., Trametes elegans, Hyalopus sp., Verticillium sp. P9, Aspergillus aculeatus DBF 9, Aspergillus niger ATTC 16620, Aspergillus niger GH1, Bacillus licheniformis KBR 6, Aspergillus niger van Tieghem, Lactobacillus plantarum ATCC 1491 T, Aspergillus niger ITCC 6514.07
-
-
-
-
?
gallotannin + H2O
gallate + D-glucose
show the reaction diagram
Penicillium chrysogenum NCIM 923
-
-
-
-
?
gallotannin + H2O
gallate + D-glucose
show the reaction diagram
Bacillus cereus KBR9
-
-
-
-
?
isoamyl gallate + H2O
gallic acid + isoamyl alcohol
show the reaction diagram
-
35% activity compared to methyl gallate
-
-
?
lauryl gallate + H2O
gallic acid + lauric acid
show the reaction diagram
-
-
-
-
?
lauryl gallate + H2O
gallate + lauric acid
show the reaction diagram
-
-
-
-
?
lauryl gallate + H2O
gallate + lauric acid
show the reaction diagram
DEHEY7
-
-
-
?
m-digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
meta-digallic acid + H2O
gallic acid
show the reaction diagram
-
-
-
-
?
meta-digallic acid + H2O
gallic acid
show the reaction diagram
-
-
-
-
?
methyl 2,4-dihydroxybenzoate + H2O
methanol + 2,4-dihydroxybenzoate
show the reaction diagram
-
-
-
-
?
methyl 2,4-dihydroxybenzoate + H2O
methanol + 2,4-dihydroxybenzoate
show the reaction diagram
DEHEY7
-
-
-
?
methyl 3,4,5-trihydroxybenzoate + H2O
methanol + 3,4,5-trihydroxybenzoate
show the reaction diagram
Aspergillus niger, Aspergillus niger GH1
-
-
-
-
?
methyl 4-hydroxybenzoate + H2O
methanol + 4-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
methyl 4-hydroxybenzoate + H2O
methanol + 4-hydroxybenzoate
show the reaction diagram
DEHEY7
-
-
-
?
methyl benzoate + H2O
methanol + benzoate
show the reaction diagram
-
-
-
-
?
methyl benzoate + H2O
methanol + benzoate
show the reaction diagram
DEHEY7
-
-
-
?
methyl benzoate + H2O
methanol + benzoate
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
-
-
-
-
?
methyl benzoate + H2O
methanol + benzoate
show the reaction diagram
DEHEY7, -
-
-
-
?
methyl gallate + 1-propanol
propyl gallate + ?
show the reaction diagram
Lactobacillus plantarum, Lactobacillus plantarum CECT 748T
-
transesterification, synthetic yields of 45% are obtained with 30% of 1-propanol at pH 5.0
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
B3Y018, -
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
Aspergillus oryzae No. 7
-
-
-
-
?
methyl gallate + H2O
gallic acid + methanol
show the reaction diagram
Talaromyces variabilis IARI 2031
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
B7VFD0, -
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
r
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Trametes elegans, Hyalopus sp.
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
C7EDT0
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
DEHEY7
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
C7F6Y1
best substrate
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
-
recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum CIR1
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Verticillium sp. P9, Aspergillus aculeatus DBF 9, Aspergillus niger ATTC 16620
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum SN35N
B3Y018
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Aspergillus niger GH1
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum CECT 748(T)
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus pentosus 21A-3
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus paraplantarum NSO120
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Aspergillus niger van Tieghem
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
B3Y018
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum ATCC 1491 T
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
DEHEY7, -
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Aspergillus awamori BTMFW032
C7F6Y1
best substrate
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Aspergillus niger ITCC 6514.07
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum CECT 748T
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Lactobacillus plantarum CECT 748T
-
recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Enterobacter sp. KPJ103
C7EDT0
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Penicillium chrysogenum NCIM 923
-
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Aspergillus niger MTCC 2425
-
-
-
?
methyl gallate + H2O
gallate + methanol
show the reaction diagram
Bacillus cereus KBR9
-
-
-
-
?
methyl gallate + H2O
methanol + gallate
show the reaction diagram
-
-
-
-
?
methyl gentisate + H2O
methanol + gentisate
show the reaction diagram
-
-
-
-
?
methyl gentisate + H2O
methanol + gentisate
show the reaction diagram
DEHEY7
-
-
-
?
methyl salicylate + H2O
methanol + salicylate
show the reaction diagram
-
-
-
-
?
methyl salicylate + H2O
methanol + salicylate
show the reaction diagram
DEHEY7
-
-
-
?
methyl vanillate + H2O
methanol + vanillate
show the reaction diagram
-
-
-
-
?
methyl vanillate + H2O
methanol + vanillate
show the reaction diagram
DEHEY7
-
-
-
?
methyl vanillate + H2O
methanol + vanillate
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
-
-
-
-
?
methyl vanillate + H2O
methanol + vanillate
show the reaction diagram
DEHEY7, -
-
-
-
?
n-propyl gallate + H2O
gallic acid + n-propanol
show the reaction diagram
-
-
-
-
?
n-propyl gallate + H2O
gallic acid + n-propanol
show the reaction diagram
-
-
-
-
?
n-propyl gallate + H2O
gallic acid + n-propanol
show the reaction diagram
Aspergillus oryzae, Aspergillus oryzae No. 7
-
55% activity compared to methyl gallate
-
-
?
n-propyl gallate + H2O
gallic acid + n-propanol
show the reaction diagram
Talaromyces variabilis IARI 2031
-
-
-
-
?
propyl 4-hydroxybenzoate + H2O
propanol + 4-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
propyl 4-hydroxybenzoate + H2O
propanol + 4-hydroxybenzoate
show the reaction diagram
DEHEY7
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
-
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
-
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
-
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
-
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
P78581
-
-
-
r
propyl gallate + H2O
gallate + propanol
show the reaction diagram
-
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
C7F6Y1
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
DEHEY7
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
E7D7J5
46% hydrolysis within 40 min
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
Aspergillus niger T3-5-1
-
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
Aspergillus awamori BTMFW032
C7F6Y1
-
-
-
?
propyl gallate + H2O
gallate + propanol
show the reaction diagram
Aspergillus niger MTCC 2425
-
-
-
?
propyl gallate + H2O
propanol + gallate
show the reaction diagram
-
-
-
-
?
propyl gallate + H2O
gallic acid + propanol
show the reaction diagram
-
-
-
-
?
propyl gallate + H2O
gallate + 1-propanol
show the reaction diagram
Lactobacillus plantarum, Lactobacillus plantarum CECT 748T
-
-
-
-
?
protocatechuic acid ethyl ester + H2O
protocatechuate + ethanol
show the reaction diagram
-
-
-
-
?
protocatechuic acid ethyl ester + H2O
ethanol + protocatechuate
show the reaction diagram
-
-
-
-
?
protocatechuic acid ethyl ester + H2O
ethanol + protocatechuate
show the reaction diagram
DEHEY7
-
-
-
?
pyrogallol + H2O
?
show the reaction diagram
-
-
-
-
?
rhodanine + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
rosmarinic acid + H2O
(2E)-3,4-dihydroxycinnamate + (2R)-3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate
show the reaction diagram
E7D7J5
88% hydrolysis within 40 min
-
-
?
tannic acid + 1-propanol
propyl gallate + ?
show the reaction diagram
Lactobacillus plantarum, Lactobacillus plantarum CECT 748T
-
transesterification, synthetic yields of 45% are obtained with 30% of 1-propanol at pH 5.0
-
-
?
tannic acid + 10 H2O
10 gallic acid + glucose
show the reaction diagram
-
-
-
?
tannic acid + 10 H2O
10 gallic acid + glucose
show the reaction diagram
-
-
-
?
tannic acid + 10 H2O
10 gallic acid + glucose
show the reaction diagram
-
-
-
?
tannic acid + 10 H2O
10 gallic acid + glucose
show the reaction diagram
-
gallotannin from fruits of Terminalia chebula
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Candida sp.
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aspergillus oryzae, Aspergillus oryzae No. 7
-
130% activity compared to methyl gallate
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aspergillus oryzae No. 7
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Klebsiella pneumoniae Q52
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aspergillus niger PKL 104
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Penicillium chrysogenum NCIM-722
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aspergillus niger LCF 8
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Candida sp. K-1
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Paenibacillus polymyxa Q47
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aspergillus aculeatus DBF9
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aureobasidium pullulans DBS66
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Corynebacterium sp. Q40
-
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Candida sp.
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
specific for
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
best substrate
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
enzyme hydrolyzes the ester and depside linkages of tannic acid
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Aspergillus aculeatus DBF 9
-
enzyme hydrolyzes the ester and depside linkages of tannic acid
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Aspergillus oryzae No. 7
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Klebsiella pneumoniae Q52
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Paenibacillus polymyxa Q47
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Aspergillus niger MTCC 2425
-
best substrate
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Corynebacterium sp. Q40
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Candida sp.
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Aspergillus oryzae No. 7
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Klebsiella pneumoniae Q52
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Aspergillus niger PKL 104
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Penicillium chrysogenum NCIM-722
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Aspergillus niger LCF 8
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Candida sp. K-1
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Paenibacillus polymyxa Q47, Corynebacterium sp. Q40
-
-
-
-
-
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Fusarium sp., Trichoderma sp.
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
tannins from Chebulina myrobalan
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Lactobacillus plantarum CECT 748(T)
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Rhizopus oryzae RO IIT RB-13
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Talaromyces variabilis IARI 2031
-
-, tannins from Chebulina myrobalan
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Aspergillus foetidus GMRB 013 MTCC 3557
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Trametes elegans, Hyalopus sp.
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
DEHEY7
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
best substrate
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
from green tea leaves
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
E7D7J5
complete hydrolysis within 40 min
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum CIR1
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Verticillium sp. P9, Aspergillus aculeatus DBF 9, Aspergillus niger ATTC 16620
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus ficuum Gim 3.6
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum SN35N
B3Y018
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger GH1
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger T3-5-1
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus foetidus MTCC 6322
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus pentosus 21A-3
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Penicillium sp. EZ-ZH390
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus paraplantarum NSO120
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger van Tieghem
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Penicillium sp. EZ-ZH190, Penicillium sp. EZ-ZH290
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
B3Y018
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Talaromyces variabilis IARI 2031
-
best substrate
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum ATCC 1491 T, Aspergillus niger ITCC 6514.07
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Penicillium chrysogenum NCIM 923
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Bacillus cereus KBR9
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Bacillus licheniformis KBR6
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
B7VFD0, -
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Hyalopus sp.
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
C7EDT0
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
C7F6Y1
lowest activity
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
at least 95% of tannic acid is transformed into pure gallate
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
highest rate of tannase activity at 1.5-2.5% (w/v) tannic acid
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Aspergillus heteromorphus MTCC 8818
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Aspergillus tamarii IMI388810 (B)
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Aspergillus niger GH1
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Hyalopus sp. DSF3
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Aspergillus awamori MTCC9299
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Aspergillus awamori BTMFW032
C7F6Y1
lowest activity
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Enterobacter sp. KPJ103
C7EDT0
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Bacillus licheniformis KBR6
-
-
-
-
?
tannic acid + n-propanol
propyl gallate
show the reaction diagram
Aspergillus awamori, Aspergillus awamori BTMFW032
-
transesterification, 3.2% conversion
-
-
?
tannin + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannin + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannin + H2O
gallate + D-glucose
show the reaction diagram
-
tannin is degraded by 55% while a combination of tannin and gelatin (1:1) results in 60% of tannin degradation
-
-
?
monogalloyl glucose + H2O
gallic acid + D-glucose
show the reaction diagram
-
37% activity compared to methyl gallate
-
-
?
additional information
?
-
-
the enzyme removes gallic acid non-specifically from both condensed and hydrolysable tannins
-
?
additional information
?
-
-
production of phytate in beans
-
?
additional information
?
-
-
the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters
-
?
additional information
?
-
-
tannase-treated green tea leaf extract shows increased inhibition activity on N-nitrosamine formation of secondary amines due to increased catechin levels compared to untreated leaf extract, the inhibitory effect is also higher as with ascorbic acid, overview
-
-
-
additional information
?
-
-
the enzyme is involved in the degradation of condensed tannins, complex tannins, gallotannins, and ellagitannins produced by different plant species in almost all tissue types, e.g. bark, wood, leaf, fruit, root and seed, overview, tannin structure and physiologic effects, degradation mechanism, and pathways, detailed overview
-
-
-
additional information
?
-
Q0KKP0, -
the tannase from Staphylococcus lugdunensis is associated with colon cancer in the human host, comparison of isolation of tannase-producing bacteria between colon cancer, adenoma, and normal groups, overview
-
-
-
additional information
?
-
-
tannase catalyzes the hydrolysis of ester and depside bonds in hydrolysable tannins or gallic acid esters liberating glucose or gallic acid
-
-
-
additional information
?
-
-
tannase cleaves ester bonds between gallic acid and glucose in tannic acid and m-digallic acid ester linkages, named depside bonds, it also hydrolyzes (+)-epicatechin gallate and (+)-epigallocatechin-3-gallate
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of ester and depside bonds in hydrolysable tannin such as tannic acid, releasing glucose and gallic acid
-
-
-
additional information
?
-
-
almost no activity with starch, D-glucose, D-sucrose, and D-xylan
-
-
-
additional information
?
-
-
tannases hydrolyze only those substrates that contain at least two phenolic OH groups in the acid component. The esterified COOH group must be on the oxidized benzene ring and must not be ortho to one of the OH groups. Benzoic esters (methyl benzoate, and ethyl benzoate), hydroxybenzoic esters (methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, and butyl 4-hydroxybenzoate), vanillic ester (methyl vanillate), gentisic ester (methyl gentisate), salicylic ester (methyl salicylate), ferulic esters (ferulic methyl ester and ferulic ethyl ester), ellagic, chlorogenic acids, quercetin, catechin, epicatechin, gallocatechin, epigallocatechin or 4-nitrophenyl beta-D-glucopyranoside are not metabolized
-
-
-
additional information
?
-
-
no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid
-
-
-
additional information
?
-
Aspergillus niger Aa-20, Bacillus licheniformis KBR 6
-
the enzyme is involved in the degradation of condensed tannins, complex tannins, gallotannins, and ellagitannins produced by different plant species in almost all tissue types, e.g. bark, wood, leaf, fruit, root and seed, overview, tannin structure and physiologic effects, degradation mechanism, and pathways, detailed overview
-
-
-
additional information
?
-
Lactobacillus plantarum CECT 748T
-
tannases hydrolyze only those substrates that contain at least two phenolic OH groups in the acid component. The esterified COOH group must be on the oxidized benzene ring and must not be ortho to one of the OH groups. Benzoic esters (methyl benzoate, and ethyl benzoate), hydroxybenzoic esters (methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, and butyl 4-hydroxybenzoate), vanillic ester (methyl vanillate), gentisic ester (methyl gentisate), salicylic ester (methyl salicylate), ferulic esters (ferulic methyl ester and ferulic ethyl ester), ellagic, chlorogenic acids, quercetin, catechin, epicatechin, gallocatechin, epigallocatechin or 4-nitrophenyl beta-D-glucopyranoside are not metabolized
-
-
-
additional information
?
-
Bacillus licheniformis KBR6
-
the enzyme catalyses the hydrolysis of ester and depside bonds in hydrolysable tannin such as tannic acid, releasing glucose and gallic acid
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
-
substrate: tannins
i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
-
substrate: tannins
i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
Verticillium sp. P9, Aspergillus aculeatus DBF 9
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Aspergillus aculeatus DBF 9
-
substrate: tannins
i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
Aspergillus niger ATTC 16620, Aspergillus niger GH1, Bacillus licheniformis KBR 6
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Bacillus licheniformis KBR 6
-
substrate: tannins
i.e. 3,4,5-trihydroxybenzoate
?
digallate + H2O
gallate
show the reaction diagram
Aspergillus niger van Tieghem, Lactobacillus plantarum ATCC 1491 T, Aspergillus niger ITCC 6514.07
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Penicillium chrysogenum NCIM 923
-
-
-
-
?
digallate + H2O
gallate
show the reaction diagram
Bacillus cereus KBR9
-
-
-
-
?
ellagitannin + H2O
hexahydroxydiphenic acid + ?
show the reaction diagram
-
-
-
-
?
ellagitannins + H2O
hexahydroxydiphenic acid + gallic acid
show the reaction diagram
Aspergillus niger, Libidibia coriaria, Aspergillus niger Aa-20
-
-
-
-
?
gallotanin + H2O
gallate + D-glucose
show the reaction diagram
DEHEY7
-
-
-
?
gallotannin + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + 10 H2O
10 gallic acid + glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
enzyme hydrolyzes the ester and depside linkages of tannic acid
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
-
the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening
-
?
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Candida sp.
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
-
-
-
-
-
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Fusarium sp., Trichoderma sp.
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Trametes elegans, Hyalopus sp.
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
DEHEY7
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
C7EDT0
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
-
at least 95% of tannic acid is transformed into pure gallate
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum CIR1
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Verticillium sp. P9, Aspergillus aculeatus DBF 9
-
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Aspergillus aculeatus DBF 9
-
enzyme hydrolyzes the ester and depside linkages of tannic acid
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger ATTC 16620
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus ficuum Gim 3.6
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum SN35N
B3Y018
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
Aspergillus oryzae No. 7
-
-
-
-
-
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger GH1
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Lactobacillus plantarum CECT 748(T)
-
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
Klebsiella pneumoniae Q52
-
-
-
-
-
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger T3-5-1
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus pentosus 21A-3
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
-
?
tannic acid + H2O
10 gallate + D-glucose
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Bacillus licheniformis KBR 6
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Penicillium sp. EZ-ZH390
-
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
Aspergillus niger PKL 104
-
-
-
-
-
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus paraplantarum NSO120
-
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
Penicillium chrysogenum NCIM-722
-
-
-
-
-
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger van Tieghem
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Penicillium sp. EZ-ZH190, Penicillium sp. EZ-ZH290
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Rhizopus oryzae RO IIT RB-13
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum ATCC 14917T
B3Y018
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Talaromyces variabilis IARI 2031
-
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
Aspergillus niger LCF 8
-
-
-
-
-
tannic acid + H2O
?
show the reaction diagram
Candida sp. K-1
-
-
-
-
-
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Lactobacillus plantarum ATCC 1491 T
-
-
-
-
?
tannic acid + H2O
gallate + ?
show the reaction diagram
Aspergillus foetidus GMRB 013 MTCC 3557
-
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
Paenibacillus polymyxa Q47
-
-
-
-
-
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Aspergillus niger ITCC 6514.07
-
-
-
-
?
tannic acid + H2O
gallic acid + D-glucose
show the reaction diagram
Enterobacter sp. KPJ103
C7EDT0
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Penicillium chrysogenum NCIM 923
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aspergillus aculeatus DBF9
-
-
-
-
?
tannic acid + H2O
gallic acid + ?
show the reaction diagram
Aureobasidium pullulans DBS66
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Bacillus cereus KBR9
-
-
-
-
?
tannic acid + H2O
gallate + D-glucose
show the reaction diagram
Bacillus licheniformis KBR6
-
-
-
-
?
tannic acid + H2O
?
show the reaction diagram
Corynebacterium sp. Q40
-
-
-
-
-
tannin + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
tannin + H2O
gallate + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
production of phytate in beans
-
?
additional information
?
-
-
the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters
-
?
additional information
?
-
-
tannase-treated green tea leaf extract shows increased inhibition activity on N-nitrosamine formation of secondary amines due to increased catechin levels compared to untreated leaf extract, the inhibitory effect is also higher as with ascorbic acid, overview
-
-
-
additional information
?
-
-
the enzyme is involved in the degradation of condensed tannins, complex tannins, gallotannins, and ellagitannins produced by different plant species in almost all tissue types, e.g. bark, wood, leaf, fruit, root and seed, overview, tannin structure and physiologic effects, degradation mechanism, and pathways, detailed overview
-
-
-
additional information
?
-
Q0KKP0, -
the tannase from Staphylococcus lugdunensis is associated with colon cancer in the human host, comparison of isolation of tannase-producing bacteria between colon cancer, adenoma, and normal groups, overview
-
-
-
additional information
?
-
Aspergillus niger Aa-20, Bacillus licheniformis KBR 6
-
the enzyme is involved in the degradation of condensed tannins, complex tannins, gallotannins, and ellagitannins produced by different plant species in almost all tissue types, e.g. bark, wood, leaf, fruit, root and seed, overview, tannin structure and physiologic effects, degradation mechanism, and pathways, detailed overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(NH4)6Mo7O24
-
0.1 M, no effect on activity
Ba2+
-
117.94% activity at 1 mM
BaCl2
-
0.1 M, no effect on activity
Br-
-
activates both isozymes TAH I and TAH II
Ca2+
-
highly stable in presence of 1 mM CaCl2
Ca2+
-
at 1 mM stimulates by 16%
Ca2+
-
1 mM increases activity by 81%
Ca2+
-
105.6% activity at 1 mM
Ca2+
E7D7J5
126% activity at 1 mM
Ca2+
-
109.51% activity at 1 mM
Ca2+
-
about 175% activity at 1 mM
Ca2+
DEHEY7
120% activity at 1 mM
Ca2+
-
1 mM Ca2+ leads to 126% and 113% activity for the free and immobilized enzyme, respectively
Ca2+
-
Ca(CH3COO)2: 33% inhibition, at 20 mM concentration
Ca2+
Candida sp.
-
Ca(CH3COO)2: no effect at a concentration of 45 mM at 30C
Ca2+
-
CaCl2: 0.1 M, no effect on activity
Ca2+
-
CaCl2, 5 mM, no effect on the activity, both the free enzyme and the immoblized enzyme
Ca2+
-
CaCl2, no effect on the activity
Cd2+
E7D7J5
121% activity at 1 mM
Co2+
-
133.59% activity at 1 mM
Co2+
-
1 mM Co2+ leads to 105% activity of the immobilized enzyme
Co2+
-
activates
Co2+
-
Co(NO3)2: 36% inhibition, at 20 mM concentration
Co2+
Candida sp.
-
CoCl2: no effect at a concentration of 45 mM at 30C
Co2+
-
0.1 M, no effect on activity
Co2+
-
CoCl2 at 5 mM, free enzyme: 49.3% inhibition, immobilized enzyme: 24% inhibition
Co2+
-
CoCl2 no effect on activity
Cu+
-
108.6% activity at 1 mM
Cu2+
-
highly stable in presence of 1 mM CuSO4
Cu2+
-
106.2% activity at 1 mM
Cu2+
E7D7J5
108% activity at 1 mM
Cu2+
-
108.44% activity at 1 mM
Cu2+
-
CuSO4: 53% inhibition, at 20 mM concentration
Cu2+
Candida sp.
-
CuSO4: no effect at a concentration of 45 mM at 30C
Cu2+
-
CuCl2: 70% inhibition, 0.1 M
Cu2+
-
CuSO4 at 5 mM, free enzyme: 59% inhibition, immobilized enzyme: 44% inhibition
Cu2+
-
CuSO4: 68% inhibition
EDTA
-
EDTA does not affect the enzyme activity
Fe2+
-
107.6% activity at 1 mM
Fe3+
C7F6Y1
144.44% activity at 10 mM
FeCl3
-
0.1 M, no effect on activity
FeCl3
-
13% inhibition
FeSO4
-
45% inhibition, at 20 mM concentration
FeSO4
-
at 5 mM, free enzyme: 61.3% inhibition, immobilized enzyme: 28% inhibition
Hg2+
-
137.73% activity at 1 mM
Hg2+
-
activates
HgCl2
-
5 mM, free enzyme: 13.7% inhibition, immobilized enzyme: 30% inhibition
K+
-
at 1 mM stimulates by 9%
K+
-
1 mM increases activity by 26%
K+
C7F6Y1
176.62% activity at 10 mM
K+
-
115.18% activity at 1 mM
Mg2+
-
activates both isozymes TAH I and TAH II
Mg2+
-
activates
Mg2+
-
at 1 mM stimulates by 23.9%
Mg2+
E7D7J5
120% activity at 1 mM
Mg2+
-
118.25% activity at 1 mM
Mg2+
-
about 120% activity at 1 mM
Mg2+
-
required for activity
Mg2+
-
110.5% activity at 1 mM
Mg2+
-
123.7% activity at 1 mM
Mg2+
-
1 mM Mg2+ leads to 120% and 110% activity for the free and immobilized enzyme, respectively
Mg2+
-
activates
Mg2+
-
MgSO4: 17% inhibition, at 20 mM concentration
Mg2+
Candida sp.
-
MgCl2, MgSO4: no effect at a concentration of 45 mM at 30C
Mg2+
-
MgCl2: no effect on activity
Mg2+
-
MgCl2 at 5 mM, free enzyme: 28.4% inhibition, immobilized enzyme: 14% inhibition
Mg2+
-
MgSO4 no effect on activity
Mn2+
-
highly stable in presence of 1 mM MnSO4
Mn2+
-
at 1 mM stimulates by 15.23%
Mn2+
-
115.8% activity at 1 mM
Mn2+
-
111.3% activity at 1 mM
Mn2+
-
activates
Mn2+
-
MnSO4: 22% inhibition, at 20 mM concentration
Mn2+
-
0.1 M, no effect on activity
Na+
-
highly stable in presence of 1 mM NaCl
Na+
-
at 1 mM stimulates by 11%
Na+
C7F6Y1
120.37% activity at 20 mM
Na+
-
124.69% activity at 1 mM
NH4+
-
activates
NH4Cl
-
110.74% activity at 1 mM
Ni2+
-
NiCl2: 42% inhibition, at 20 mM concentration
Ni2+
Candida sp.
-
NiSO4, no effect at a concentration of 45 mM at 30C
Ni2+
-
NiSO4: 0.1 M, no effect on activity
Urea
-
activates both isozymes TAH I and TAH II
Zn2+
-
142% activity at 1 mM
Zn2+
-
1 mM Zn2+ leads to 108% activity of the immobilized enzyme
Zn2+
-
activates
Zn2+
-
ZnCl2: 22% inhibition, ZnSO4: 45% inhibition, at 20 mM concentration
Zn2+
Candida sp.
-
no effect of ZnCl2 and ZnSO4 at a concentration of 45 mM at 30C
Zn2+
-
ZnCl2 70% inhibition, 0.1 M
Zn2+
-
ZnCl2 at 5 mM, free enzyme: 55.4% inhibition, immobilized enzyme: 29.3% inhibition
Zn2+
-
ZnCl2 39% inhibition
Mn2+
-
MnCl2 no effect on activity
additional information
-
-
additional information
-
no effect at 1 mM by K+, Ca2+, Zn2+, and urea
additional information
-
1 mM EDTA has no significant effect
additional information
-
Ba2+ has no significant effect on tannase activity up to 3 mM
additional information
E7D7J5
the enzyme is not influenced by Zn2+ and Co2+
additional information
-
not affected by K+
additional information
-
enzyme activity is not affected significantly by most ions
additional information
-
not affected by Mg2+, Mn2+, Ca2+, and Zn2+
additional information
-
no effect of the following metal ions at a concentration of 0.1 M: KCl, AlCl3, CdCl2 and SrCl2
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
slight inhibition, 1 mM, 88% remaining activity
1,10-phenanthroline
-
-
1,10-phenanthroline
-
40% residual activity at 1 mM
1,10-phenanthroline
-
42% inhibition at 1 mM
1,2,3-trihydroxybenzoic acid
-
competitive; i.e. pyrogallol
-
1,2-dihydroxybenzene
-
competitive
1,3-dihydroxybenzene
-
competitive
1,4-dihydroxybenzene
-
competitive
1-propanol
-
activates at a concentration of 3.6-7.3% v/v, at higher concentration it inhibits the propyl gallate synthesis reaction causing disruption of essential membrane functions and denaturation of enzyme
1-propanol
-
inhibits the enzymatic hydrolysis of propyl gallate
2,3-Dihydroxybenzoic acid
-
competitive
2,5-dihydroxybenzoic acid
-
competitive
2,6-dihydroxybenzoic acid
-
noncompetitive
2-hydroxybenzoic acid
-
competitive
2-mercaptoethanol
-
1 mM, 68.3% remaining activity
2-mercaptoethanol
-
inhibits both isozymes TAH I and TAH II
2-mercaptoethanol
-
41% inhibition at 1 mM
2-mercaptoethanol
-
39% inhibition at 1 mM
2-mercaptoethanol
DEHEY7
16% residual activity at 1 mM
2-mercaptoethanol
-
-
3,4-dihydroxybenzoic acid
-
competitive
3,5-Dihydroxybenzoic acid
-
competitive
3-Hydroxybenzoic acid
-
competitive
4-Aminobenzoic acid
-
25% inhibition at 1 mM
4-Aminobenzoic acid
-
81.9% residual activity at 1 mM
4-chloromercuribenzoate
-
inhibits both isozymes TAH I and TAH II
4-chloromercuribenzoate
-
-
4-hydroxybenzoic acid
-
competitive
8-hydroxyquinoline
-
47.2% residual activity at 1 mM
acetic acid
-
completely inhibits activity
acetone
-
complete inactivation
acetone
-
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 55.01%
acetone
-
at 60% of concentration, completely inhibits activity at 30C
Ag+
-
slight competitive inhibition at 1 mM, 82.4% remaining activity
Ag+
-
inhibits both isozymes TAH I and TAH II
Ag+
-
57% residual activity at 5 mM
Ag+
E7D7J5
51% residual activity at 1 mM
AgNO3
-
98.77% residual activity at 1 mM
Al3+
E7D7J5
97% residual activity at 1 mM
Al3+
C7F6Y1
complete inhibition at 1 mM
Al3+
-
75.14% residual activity at 1 mM
alpha-glutathione
-
inhibits both isozymes TAH I and TAH II
Ba2+
-
competitive inhibition at 1 mM, 5.4% remaining activity
Ba2+
-
competitive inhibitor
Ba2+
C7F6Y1
complete inhibition at 5 mM
Benzene
-
98% residual activity at 5% (v/v)
Benzoic acid
-
83.1% residual activity at 1 mM
beta-mercaptoethanol
-
-
beta-mercaptoethanol
-
1 mM inhibits activity by 85%
beta-mercaptoethanol
-
59.4% residual activity at 1 mM
beta-mercaptoethanol
E7D7J5
63% residual activity at 1 mM
beta-mercaptoethanol
C7F6Y1
-
beta-mercaptoethanol
-
37.07% residual activity at 20 mM
beta-mercaptoethanol
-
about 10% residual activity at 1 mM
Bromoacetic acid
-
-
Bromoacetic acid
-
53.3% residual activity at 1 mM
Ca2+
-
58% inhibition at 20 mM, noncompetitive
Ca2+
-
slight competitive inhibition at 1 mM, 86.6% remaining activity
Ca2+
-
7% inhibition of tannase produced under submerged fermentation at 1 mM
Ca2+
C7F6Y1
0.84% residual activity at 20 mM
Ca2+
-
88.7% residual activity at 1 mM
Ca2+
-
92.3% residual activity at 1 mM
Ca2+
-
98.3% residual activity at 1 mM
CaCl2
-
noncompetitive
carbon tetrachloride
-
complete inactivation
Cd2+
-
55% inhibition at 20 mM, noncompetitive
Cd2+
-
inhibits both isozymes TAH I and TAH II
Cd2+
B7VFD0, -
45% inhibition, at 30C in sodium citrate buffer, pH 6.0
Cd2+
-
57% residual activity at 5 mM
Cd2+
C7F6Y1
11.82% residual activity at 20 mM
Chloroform
-
completely inhibits activity
Co2+
-
complete inhibition at 1 mM
Co2+
-
inhibits both isozymes TAH I and TAH II
Co2+
-
at 1 mM inhibits by 71.14%
Co2+
-
competitive inhibitor
Co2+
-
87.6% residual activity at 1 mM
Co2+
C7F6Y1
39.06% residual activity at 20 mM
CO32-
-
inhibits both isozymes TAH I and TAH II
Cr2+
E7D7J5
52% residual activity at 1 mM
Cu2+
-
inhibits both isozymes TAH I and TAH II
Cu2+
-
at 1 mM inhibits by 51.21%
Cu2+
-
ca. 20% inhibition after 60 min of incubation at 30C and pH 5
Cu2+
-
competitive inhibitor
Cu2+
B7VFD0, -
51% inhibition, at 30C in sodium citrate buffer, pH 6.0
Cu2+
-
41% inhibition of tannase produced under solid-state fermentation at 1 mM
Cu2+
C7F6Y1
10.58% residual activity at 20 mM
Cu2+
-
mild inhibitory effect
cyanamide
-
61.7% residual activity at 1 mM
cysteine
-
38% inhibition at 1 mM
D-glucose
-
slightly stimulating, inhibits intracellular enzyme production at concentrations above 0.05% w/v, and extracellular enzyme production above 0.1% w/v
diisopropylfluorophosphate
-
DFP, inhibition is not immediate, but requires a period of preincubation of the enzyme. 1 mol of 32P of DFP is incorporated into 1 mol of enzyme to give complete inhibition, suggest that the enzyme contains one essential serine per mol enzyme, a typical serine enzyme
diisopropylfluorophosphate
-
83% inhibition
dithiothreitol
-
54.1% residual activity at 1 mM
dithiothreitol
C7F6Y1
displays inhibitory properties at higher concentrations of 1.0% to 5.0% (v/v)
DMSO
-
1 mM, 19% remaining activity
DMSO
-
most potent inhibitor
EDTA
-
1 mM, 30% remaining activity
EDTA
-
complete inhibition at 5 mM
EDTA
-
38% inhibition at 1 mM
EDTA
-
at 0.01% of concentration 22% inhibition after 5 min and at 0.1% of concentration 19% inhibition after 60 min at 30C
EDTA
-
35% inhibition at 1 mM
EDTA
E7D7J5
86% residual activity at 1 mM
EDTA
-
25.89% residual activity at 20 mM
EDTA
-
about 30% residual activity at 1 mM
EDTA
DEHEY7
80% residual activity at 1 mM
EDTA
-
the enzyme is partially inhibited by but not significantly affected (87.8% residual activity at 1 mM)
EDTA
-
the enzyme is partially inhibited by but not significantly affected (97.6% residual activity at 1 mM)
EDTA
-
5 mM EDTA is completely inhibitory for tannase activity
EDTA
Candida sp.
-
95% remaining activity after 3 days at a concentration of 10 mM, pH 7.2, 0.1 M phosphate buffer
EDTA
-
activity is completely lost, when the enzyme is dialyzed against 0.025 M EDTA
ethanol
-
inhibits by 48.84% initially, thereafter complete loss in the enzyme activity at 40% and 60%
ethanol
-
at 60% of concentration, completely inhibits activity at 30C
ethanol
-
80.2% residual activity at 5% (v/v)
ethyl gallate
-
20% inhibition at 1 mM
ethyl gallate
-
1 mM inhibits activity by 48%
Fe2+
-
63% inhibition of tannase produced under submerged fermentation at 1 mM
Fe2+
-
24.1% residual activity at 1 mM
Fe2+
-
23.4% residual activity at 1 mM
Fe2+
-
22.5% residual activity at 1 mM
Fe3+
-
complete inhibition at 1 mM
Fe3+
-
inhibits both isozymes TAH I and TAH II
Fe3+
-
at 1 mM inhibits by 76.89%
Fe3+
-
more than 40% inhibition after 5 min and 60 min of incubation at 30C and pH 5
Fe3+
-
competitive inhibitor
Fe3+
B7VFD0, -
35% inhibition, at 30C in sodium citrate buffer, pH 6.0
Fe3+
-
85.74% residual activity at 1 mM
Fe3+
-
highly inhibited by Fe3+
formaldehyde
-
complete inactivation
formaldehyde
-
51.5% inhibition at 20% after 60 min at 30C
gallate
-
inhibits the enzymatic hydrolysis of propyl gallate
gallic acid
-
activates at a concentration up to 0.005 mM, at higher concentration it inhibits the propyl gallate synthesis reaction
gallic acid
B7VFD0, -
competitive inhibition using gallic acid as substrate
gallic acid
-
82.1% residual activity at 1 mM
gallic acid
-
competitive; i.e. 3,4,5-trihydroxybenzoic acid
glutathione
-
-
H2O2
C7F6Y1
H2O2 concentrations of less than 2% and higher than 10% lead to a decline in enzyme activity
heptane
-
at 60% of concentration 95% inhibition after 60 min at 30C
Hg2+
-
competitive inhibition at 1 mM, 13.8% remaining activity
Hg2+
-
inhibits both isozymes TAH I and TAH II
Hg2+
-
66% inhibition at 1 mM
Hg2+
-
1 mM inhibits activity by 78%
Hg2+
-
56% residual activity at 5 mM
Hg2+
E7D7J5
complete inhibition at 1 mM
Hg2+
C7F6Y1
53.02% residual activity at 20 mM
Hg2+
-
complete inhibition at 1 mM
Hg2+
DEHEY7
6% residual activity at 1 mM
Hg2+
-
complete inhibition of the free enzyme at 1 mM, the immobilized enzyme shows 93.7% residual activity at 1 mM
Hg2+
-
efficient inhibitor
iodoacetic acid
-
-
iodoacetic acid
-
48% residual activity at 1 mM
Isoamylalcohol
-
completely inhibits activity
Isopropanol
-
96.3% residual activity at 5% (v/v)
Isopropyl alcohol
-
completely inhibits activity
K+
-
inhibits both isozymes TAH I and TAH II
K+
-
73.3% residual activity at 5 mM
K+
DEHEY7
69% residual activity at 1 mM
Li+
C7F6Y1
3.07% residual activity at 20 mM
mercuribenzoic acid
-
48.8% residual activity at 1 mM
mercuric benzoic acid
-
-
-
methanol
-
initial inhibitory effect at 20% and 40%, original activity is regained at 60%
methanol
-
95.8% residual activity at 5% (v/v)
Mg2+
-
33% inhibition at 20 mM, noncompetitive
Mg2+
-
16% inhibition at 1 mM
Mg2+
-
slight reduction in activity in the presence of Mg2+
Mg2+
-
1 mM inhibits activity by 12%
Mg2+
-
ca. 20% inhibition after 5 min of incubation at 30C and pH 5
Mg2+
-
77.5% residual activity at 5 mM
Mg2+
C7F6Y1
7.03% residual activity at 20 mM
Mg2+
DEHEY7
65% residual activity at 1 mM
Mg2+
-
96.7% residual activity at 1 mM
Mn2+
-
54% inhibition at 20 mM, noncompetitive
Mn2+
-
inhibits both isozymes TAH I and TAH II
Mn2+
-
ca. 20% inhibition after 5 min of incubation at 30C and pH 5
Mn2+
-
79% residual activity at 1 mM
Mn2+
E7D7J5
90% residual activity at 1 mM
Mn2+
C7F6Y1
10.18% residual activity at 20 mM
Mn2+
-
75.6% residual activity at 1 mM
Mn2+
-
87.6% residual activity at 1 mM
N-bromosuccinimide
-
29% inhibition at 1 mM
N-bromosuccinimide
-
73.9% residual activity at 1 mM
N-ethylmaleimide
-
7% residual activity at 1 mM
N-ethylmaleimide
-
-
Na+
-
89.1% residual activity at 5 mM
NaCl
-
inhibits above 3 M
NaCl
E7D7J5
the enzyme retains 80% activity at 5 M
NEM
-
81% inhibition
Pb2+
-
inhibits both isozymes TAH I and TAH II
petroleum ether
-
-
-
phenyl boronic acid
-
-
phenyl boronic acid
-
67.1% residual activity at 1 mM
phenyl methyl sulphonyl fluoride
B7VFD0, -
95% inhibition, at 30C in sodium citrate buffer, pH 6.0
phenylmethylsulfonyl fluoride
-
28% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
38% inhibition after 5 min of incubation at 30C and pH 5, indicating the presence of a serine or cysteine residue in the catalytic site
phenylmethylsulfonyl fluoride
-
86.1% residual activity at 1 mM
phenylmethylsulfonyl fluoride
E7D7J5
32% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
the enzyme is partially inhibited by but not significantly affected (66.2% residual activity at 1 mM)
phenylmethylsulfonyl fluoride
-
81.2% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
the enzyme is partially inhibited by but not significantly affected (83.2% residual activity at 1 mM)
PMSF
-
72% inhibition
propranolol hydrochloride
-
-
propranolol hydrochloride
-
49.6% residual activity at 1 mM
propyl gallate
-
85.1% residual activity at 1 mM
pyrogallol
-
95% residual activity at 1 mM
SDS
-
complete inactivation
SDS
-
at 0.01% of concentration 15% inhibition after 60 min at 30C
SDS
-
2% (w/v) SDS is completely inhibitory for tannase activity
Sn2+
-
inhibits both isozymes TAH I and TAH II
Sodium azide
-
36% inhibition at 1 mM
Sodium azide
-
82.8% residual activity at 1 mM
Sodium bisulfite
-
25% inhibition at 1 mM
Sodium bisulfite
-
81.3% residual activity at 1 mM
Sodium cholate
-
52.6% residual activity at 1 mM
sodium dodecyl sulphate
-
inhibits isozyme TAH II
sodium lauryl sulfate
-
-
sodium lauryl sulfate
-
above 2%
Sodium thioglycolate
-
83.1% residual activity at 1 mM
soybean extract
-
inhibits at 0.05-1.0% w/v
-
tetrahydrofuran
-
complete inactivation
tetrahydrofuran
-
at 60% of concentration, completely inhibits activity at 30C
Toluene
-
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 28.49%
Triton X 100
-
1 mM inhibits activity by 20%
Triton X-100
-
inhibits both isozymes TAH I and TAH II
Triton X-100
-
48% inhibition at 1% v/v
Triton X-100
-
32% inhibition at 1 mM
Triton X-100
-
21.9% inhibition at 1% (v/v)
Triton X-100
DEHEY7
78% residual activity at 1 mM
Triton X-100
-
-
Tween
-
1 mM inhibits activity by 20%
Tween 20
-
inhibits both isozymes TAH I and TAH II
Tween 20
-
30% inhibition at 1% v/v
Tween 20
-
30.8% inhibition at 1% (v/v)
Tween 40
-
activation up to 0.05% v/v, complete inhibition at 0.6% v/v; activation up to 0.4% v/v, complete inhibition at 0.6% v/v
Tween 60
-
complete inhibition at 1% v/v
Tween 60
-
inhibits both isozymes TAH I and TAH II
Tween 80
-
inhibits both isozymes TAH I and TAH II
Tween 80
-
30% inhibition at 1% v/v
Tween 80
-
at 0.01% of concentration 35% inhibition after 5 min at 30C
Tween 80
-
27.4% inhibition at 1% (v/v)
Tween 80
E7D7J5
47% residual activity at 1 mM
Tween-60
-
complete inactivation
Tween-80
-
complete inactivation
Urea
-
at 1.5 M maximal enhancement of activity, at 3 M 50% inhibition
Urea
-
slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M
Urea
-
1 mM inhibits activity by 52%
Urea
DEHEY7
35% residual activity at 1 mM
Urea
-
the enzyme is partially inhibited by but not significantly affected (96.9% residual activity at 1 mM)
Urea
-
the enzyme is partially inhibited by but not significantly affected (91.4% residual activity at 1 mM)
Urea
-
urea concentrations higher than 3 M are completely inhibitory for tannase activity
Zn2+
-
41% inhibition at 20 mM, noncompetitive
Zn2+
-
competitive inhibition at 1 mM, 21.3% remaining activity
Zn2+
-
inhibits both isozymes TAH I and TAH II
Zn2+
-
slight reduction in activity in the presence of Zn2+
Zn2+
-
1 mM inhibits activity by 18%
Zn2+
-
ca. 10% inhibition after 5 min and 60 min of incubation at 30C and pH 5
Zn2+
-
competitive inhibitor
Zn2+
-
80% residual activity at 5 mM
Zn2+
C7F6Y1
30.54% residual activity at 20 mM
Zn2+
-
about 65% residual activity at 1 mM
Zn2+
DEHEY7
10% residual activity at 1 mM
Zn2+
-
95.4% residual activity at 1 mM
Zn2+
-
25.2% residual activity at 1 mM
Zn2+
-
46.1% residual activity at 1 mM
Zn2+
-
; mild inhibitory effect
Mo2+
C7F6Y1
22.48% residual activity at 20 mM
additional information
-
no inhibition of the extracellular enzyme by PMSF
-
additional information
-
not affected by EDTA
-
additional information
-
no inhibition by Triton X-100
-
additional information
-
no inhibition at 1 mM iodoacetamide
-
additional information
-
-
-
additional information
-
no effect at 1 mM by K+, Ca2+, Zn2+, Tween 80, urea, DMSO, and EDTA
-
additional information
-
induction and repression patterns, overview
-
additional information
-
the enzyme is not affected by EDTA
-
additional information
-
1 mM Zn2+ has no effect
-
additional information
-
1 mM DMSO has no significant effect
-
additional information
-
at 0.1% and 0.01% of concentration, Ca2+, Tween 20 and Triton X-100 have no effect at 30C
-
additional information
B7VFD0, -
EDTA, Mg2+, Mn2+, Ca2+, Zn2+ and EDTA have no or only weak effects
-
additional information
-
not inhibited by iodoacetamide
-
additional information
E7D7J5
the enzyme is not inhibited by 1-4 M NaCl
-
additional information
-
not inhibited by EDTA
-
additional information
-
not inhibited by benzoic acid and 3-methyl-benzoic acid
-
additional information
-
not inhibited by o-phenanthroline, PMSF, EDTA, 2-mercaptomethanol, sodium thioglycolate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-propanol
-
activates at a concentration of 3.6-7.3% v/v, at higher concentration it inhibits the propyl gallate synthesis reaction causing disruption of essential membrane functions and denaturation of enzyme
ammonium chloride
-
0.1% w/v, 4fold increased activity when used as nitrogen source
ammonium nitrate
-
0.1% w/v, 1.2fold increased activity when used as nitrogen source
ammonium sulfate
-
with FeSO4, when used as nitrogen source, 1.7fold increase in activity
Benzene
-
optimum enzyme concentration should be 0.1 g mycelial dry weight per 10 ml benzene
Benzene
-
increases activity by 2fold at 60% v/v concentration
Br-
-
1.6fold induction
butanol
-
increases activity by 2fold at 60% v/v concentration
D-glucose
-
glucose has a stimulatory effect on tannase synthesis in vivo at 0.1% w/v concentration
dithiothreitol
C7F6Y1
enhances activity at low concentrations of 0.2% (v/v) (108.1% residual activity) to 0.6% (v/v) (111.8% residual activity)
DMSO
-
about 130% activity at 1 mM
DMSO
DEHEY7
144% activity at 1 mM
EDTA
-
103.7% activity at 1 mM
EDTA
-
1 mM EDTA leads to 106% activity of the immobilized enzyme
gallic acid
-
activates at a concentration up to 0.005 mM, at higher concentration it inhibits the propyl gallate synthesis reaction
heptane
-
at 20% of concentration increases activity by 19% after 60 min, and at 60% of concentration by 97% after 6 min
Hg+
-
slight activation at 1 mM, 105% activity
Mg2+
-
activation at 1 mM, 137.9% activity
N,N-Dimethylformamide
-
-
petroleum ether
-
at 20% of concentration increases activity by 19% after 60 min, and at 60% of concentration by 73% after 6 min
-
S2O32-
-
2.2fold induction
SDS
-
138.96% activity at 0.01% (v/v)
SDS
-
1% (v/v) SDS shows stimulatory (13.2%) effect on tannase activity
sodium lauryl sulfate
-
45% increase of activity at 1% (v/v)
sodium taurocholate
-
-
Sodium thioglycolate
C7F6Y1
an increase in residual enzyme activity from 23.1% (0.2% concentration) to a maximum of 321.2% (1.0% concentration) is noted along with an increase in its concentration
Tannic acid
-
required for enzyme induction, best at 1% w/v tannic acid for the intracellular enzyme, and at 2% w/v for the extracellular enzyme
Tannic acid
-
enzyme is inducible
Tannic acid
-
maximum enzyme production (9.38 U/ml) is recorded after 96 h of incubation at 35C, initial pH 5, in submerged culture (200 rpm) utilising 2% (w/v) tannic acid as a sole carbon source
Triton X-100
-
121.63% activity at 0.01% (v/v)
Triton X-100
-
about 150% activity at 1 mM
Tween 40
-
best at 0.4% v/v, above that concentration it inhibits the enzyme
Tween 80
-
best at 0.05% v/v, above that concentration it inhibits the enzyme
Tween 80
-
at 0.1% of concentration increases activity by 25% after 60 min
Tween 80
DEHEY7
119% activity at 1 mM
Urea
-
at 1.5 M maximal enhancement of activity, at 3 M 50% inhibition
Urea
-
slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M
Urea
E7D7J5
106% activity at 1 mM
Urea
-
119.5% activity at 1 mM
Urea
-
1 mM urea leads to 106% and 113% activity for the free and immobilized enzyme, respectively
additional information
-
not affected by EDTA
-
additional information
-
no effect at 1 mM by Tween 80, DMSO, and EDTA
-
additional information
-
induction and repression patterns, overview
-
additional information
-
the enzyme is not affected by EDTA
-
additional information
-
poor activating effects by octane and dodecane, tannic acid induces the enzyme, effects of organic solvents on propyl gallate production by the enzyme, and effect of water content, overview
-
additional information
-
Tween 20 and Triton X-100 have no effect
-
additional information
-
addition of 0.5% (w/v) green tea extract increases enzyme activity
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5.9
-
1,2,3,4,6-pentagalloyl glucose
-
-
6.9
-
1,2,3,6-tetra-O-galloyl-beta-D-glucose
-
-
2.3
-
1,2,6-Tri-O-galloyl-beta-D-glucose
-
-
1.3
-
1,6-di-O-galloyl-beta-D-glucose
-
-
12.7
-
1-O-anisoyl-beta-D-glucose
-
-
2
-
1-O-benzoyl-beta-D-glucose
-
-
0.14
-
1-O-Galloyl-beta-D-glucose
-
-
9.1
-
1-O-Galloyl-beta-D-glucose
-
-
2.1
-
1-O-p-hydroxybenzoyl-beta-D-glucose
-
-
6
-
1-O-Protocatechuoyl-beta-D-glucose
-
-
0.85
-
1-O-syringoyl-beta-D-glucose
-
-
17.5
-
1-O-vanilloyl-beta-D-glucose
-
-
4
-
1-O-Veratroyl-beta-D-glucose
-
-
5.5
-
3,6-di-O-galloyl-beta-D-glucose
-
-
6
-
4-O-digalloyl-1,2,3,6-tetra-O-galloyl-beta-D-glucose
-
-
-
4.7
-
6-O-galloyl-beta-D-glucose
-
-
0.05
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.08
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.45
-
catechin gallate
-
native enzyme, at pH 5.0 and 30C
0.47
-
catechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
4.1
-
chlorogenic acid
-
-
0.03
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.05
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.06
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.23
-
Epicatechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
0.34
-
Epicatechin gallate
-
native enzyme, at pH 5.0 and 30C
0.06
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.1
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.26
-
epigallocatechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
0.32
-
epigallocatechin gallate
-
native enzyme, at pH 5.0 and 30C
0.005
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.04
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.86
-
ethyl gallate
-
native enzyme, at pH 5.0 and 30C
1.3
-
ethyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
2.3
-
ethyl gallate
-
-
0.03
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.06
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.07
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.7
-
m-digallic acid
-
tannase II
0.99
-
m-digallic acid
-
-
2
-
m-digallic acid
-
tannase I
0.41
-
Methyl 3,4,5-trihydroxybenzoate
-
at 60C
0.37
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.433
-
methyl gallate
-
free enzyme, in citrate buffer (50 mM, pH 5.5), at 30C
0.5
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.529
-
methyl gallate
-
Ca alginate-immobilized enzyme, in citrate buffer (50 mM, pH 5.5), at 30C
0.62
-
methyl gallate
B3Y018
at pH 8.0
0.62
-
methyl gallate
-
wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37C
0.78
-
methyl gallate
-
enzyme produced under solid-state fermentation, at pH 7.0, 30C
0.86
-
methyl gallate
-
-
0.87
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.95
-
methyl gallate
-
-
0.95
-
methyl gallate
-
mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37C
1
-
methyl gallate
-
mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37C
1.02
-
methyl gallate
-
native enzyme, at pH 5.0 and 30C
1.6
-
methyl gallate
-
pH and temperature not specified in the publication
1.6
-
methyl gallate
-
-
1.7
-
methyl gallate
-
tannase I
1.82
-
methyl gallate
-
at 45C, in 50 mM citrate buffer at pH 5.0
1.9
-
methyl gallate
C7F6Y1
at pH 2.0, 30C
1.97
-
methyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
2.06
-
methyl gallate
-
at 40C, in 50 mM citrate buffer at pH 5.0
3.5
-
methyl gallate
B7VFD0, -
recombinant tannase, at 30C, pH 6.0
3.7
-
methyl gallate
-
pH and temperature not specified in the publication
4.06
-
methyl gallate
-
at 50C, in 50 mM citrate buffer at pH 5.0
4.35
-
methyl gallate
-
at 55C, in 50 mM citrate buffer at pH 5.0
4.4
-
methyl gallate
B7VFD0, -
endogenous tannase, at 30C, pH 6.0
4.47
-
methyl gallate
-
at 60C, in 50 mM citrate buffer at pH 5.0
4.78
-
methyl gallate
-
pH 5.0, 45C
4.94
-
methyl gallate
-
at 20C, in 50 mM citrate buffer at pH 5.0
5.06
-
methyl gallate
-
at 35C, in 50 mM citrate buffer at pH 5.0
5.11
-
methyl gallate
-
at 25C, in 50 mM citrate buffer at pH 5.0
5.17
-
methyl gallate
-
at 30C, in 50 mM citrate buffer at pH 5.0
6.2
-
methyl gallate
-
tannase II
7.41
-
methyl gallate
-
enzyme produced under submerged fermentation, at pH 7.0, 30C
12.05
-
methyl gallate
-
at 65C, in 50 mM citrate buffer at pH 5.0
14
-
methyl gallate
-
in 0.5 M citrate phosphate buffer, pH 5.0, at 50C
0.85
-
propyl gallate
-
at pH 5.0 and 45C
1.12
-
propyl gallate
-
native enzyme, at pH 5.0 and 30C
1.38
-
propyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
2.05
-
propyl gallate
-
-
2.4
-
propyl gallate
-
-
7.69
-
propyl gallate
-
pH 5.0, 45C
12
-
propyl gallate
-
in 0.5 M citrate phosphate buffer, pH 5.0, at 50C
16.94
-
pyrogallol
-
pH 5.0, 45C
0.000011
-
Tannic acid
-
free enzyme, pH 5.0, 30C
0.000041
-
Tannic acid
-
immobilized enzyme, pH 6.0, 40C
0.00061
-
Tannic acid
-
pH 5.5, 60C
0.00337
-
Tannic acid
-
pH and temperature not specified in the publication
0.048
-
Tannic acid
-
-
0.05
-
Tannic acid
-
-
0.14
-
Tannic acid
B7VFD0, -
endogenous tannase, at 30C, pH 6.0
0.17
-
Tannic acid
B7VFD0, -
recombinant tannase, at 30C, pH 6.0
0.21
-
Tannic acid
-
pH 5.0, 40C
0.23
-
Tannic acid
-
at 60C
0.28
-
Tannic acid
-
-
0.28
-
Tannic acid
-
-
0.4
-
Tannic acid
-
free enzyme, in citrate buffer (50 mM, pH 5.5), at 30C
0.445
-
Tannic acid
-
pH and temperature not specified in the publication
0.49
-
Tannic acid
-
enzyme produced under submerged fermentation, at pH 7.0, 30C
1.05
-
Tannic acid
-
isozyme TAH I, pH 5.5, 20C
1.4
-
Tannic acid
-
enzyme produced under solid-state fermentation, at pH 7.0, 30C
1.51
-
Tannic acid
-
isozyme TAH II, pH 5.5, 20C
3.3
-
Tannic acid
-
purified enzyme, at pH 5.0 and 37C
3.57
-
Tannic acid
-
purified enzyme
5.5
-
Tannic acid
-
at pH 5.5, 37C
6.6
-
Tannic acid
-
crude enzyme, at pH 5.0 and 37C
14.01
-
Tannic acid
-
pH 5.0, 45C
23.75
-
Tannic acid
-
Ca alginate-immobilized enzyme, in citrate buffer (50 mM, pH 5.5), at 30C
32
-
Tannic acid
-
pH 5.0, 50C
32
-
Tannic acid
-
in 0.5 M citrate phosphate buffer, pH 5.0, at 50C
0.00061
-
tannin
-
-
-
71.4
-
methyl gallate
-
-
additional information
-
additional information
-
kinetics
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.48
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
2.41
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
8.1
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
64.5
-
catechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
79.8
-
catechin gallate
-
native enzyme, at pH 5.0 and 30C
0.42
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1.49
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
11.08
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
67.5
-
Epicatechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
103.4
-
Epicatechin gallate
-
native enzyme, at pH 5.0 and 30C
0.44
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1.12
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
14.29
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
62.1
-
epigallocatechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
86.1
-
epigallocatechin gallate
-
native enzyme, at pH 5.0 and 30C
0.06
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.26
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.35
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
9.8
-
ethyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
19
-
ethyl gallate
-
native enzyme, at pH 5.0 and 30C
0.89
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1.12
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
9.2
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
0.53
-
methyl gallate
-
mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37C
2.5
-
methyl gallate
-
mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37C
15.95
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
17.02
-
methyl gallate
-
at 20C, in 50 mM citrate buffer at pH 5.0
46.02
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
52.48
-
methyl gallate
-
at 25C, in 50 mM citrate buffer at pH 5.0
60.2
-
methyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
60.99
-
methyl gallate
-
at 35C, in 50 mM citrate buffer at pH 5.0
70.2
-
methyl gallate
-
native enzyme, at pH 5.0 and 30C
72.73
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
74
-
methyl gallate
-
wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37C
78.01
-
methyl gallate
-
at 30C, in 50 mM citrate buffer at pH 5.0
580.1
-
methyl gallate
-
at 40C, in 50 mM citrate buffer at pH 5.0
601.4
-
methyl gallate
-
at 45C, in 50 mM citrate buffer at pH 5.0
1109
-
methyl gallate
-
at 50C, in 50 mM citrate buffer at pH 5.0
1364
-
methyl gallate
-
at 55C, in 50 mM citrate buffer at pH 5.0
1564
-
methyl gallate
-
at 60C, in 50 mM citrate buffer at pH 5.0
2611
-
methyl gallate
-
at 65C, in 50 mM citrate buffer at pH 5.0
18.6
-
propyl gallate
-
native enzyme, at pH 5.0 and 30C
20.5
-
propyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
19.22
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
55330
53.65
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
55330
137.3
-
catechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
55330
179.3
-
catechin gallate
-
native enzyme, at pH 5.0 and 30C
55330
181.5
-
catechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
55330
8.63
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
3466
52.23
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
3466
195.3
-
Epicatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
3466
293.6
-
Epicatechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
3466
308.8
-
Epicatechin gallate
-
native enzyme, at pH 5.0 and 30C
3466
7.25
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1234
11.68
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1234
234
-
epigallocatechin gallate
-
recombinant enzyme, at pH 5.0 and 30C
1234
260.8
-
epigallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1234
273.4
-
epigallocatechin gallate
-
native enzyme, at pH 5.0 and 30C
1234
6.04
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
197668
9.02
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
197668
10.57
-
epigallocatechin-3-O-(3-O-methyl) gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
197668
7.54
-
ethyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
11000
22.1
-
ethyl gallate
-
native enzyme, at pH 5.0 and 30C
11000
14.32
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
6836
27.19
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
6836
154.7
-
gallocatechin gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
6836
0.021
-
methyl gallate
-
mutant enzyme K343A, in 25 mM Tris-HCl (pH 8.0), at 37C
1402
0.112
-
methyl gallate
B7VFD0, -
recombinant tannase, at 30C, pH 6.0
1402
0.127
-
methyl gallate
B7VFD0, -
endogenous tannase, at 30C, pH 6.0
1402
0.52
-
methyl gallate
-
mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37C
1402
2.7
-
methyl gallate
-
mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37C
1402
3.45
-
methyl gallate
-
at 20C, in 50 mM citrate buffer at pH 5.0
1402
10.26
-
methyl gallate
-
at 25C, in 50 mM citrate buffer at pH 5.0
1402
12.06
-
methyl gallate
-
at 35C, in 50 mM citrate buffer at pH 5.0
1402
15.08
-
methyl gallate
-
at 30C, in 50 mM citrate buffer at pH 5.0
1402
18.79
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1402
30.6
-
methyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
1402
69.2
-
methyl gallate
-
native enzyme, at pH 5.0 and 30C
1402
120
-
methyl gallate
-
wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37C
1402
125
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1402
145.1
-
methyl gallate
-
in 50 mM Tris-HCl (pH 8.0), at 37C
1402
216.7
-
methyl gallate
-
at 65C, in 50 mM citrate buffer at pH 5.0
1402
273.5
-
methyl gallate
-
at 50C, in 50 mM citrate buffer at pH 5.0
1402
282
-
methyl gallate
-
at 40C, in 50 mM citrate buffer at pH 5.0
1402
313.7
-
methyl gallate
-
at 55C, in 50 mM citrate buffer at pH 5.0
1402
330
-
methyl gallate
-
at 45C, in 50 mM citrate buffer at pH 5.0
1402
350.2
-
methyl gallate
-
at 60C, in 50 mM citrate buffer at pH 5.0
1402
14.9
-
propyl gallate
-
recombinant enzyme, at pH 5.0 and 30C
1879
16.6
-
propyl gallate
-
native enzyme, at pH 5.0 and 30C
1879
0.064
-
Tannic acid
B7VFD0, -
recombinant tannase, at 30C, pH 6.0
1019
0.078
-
Tannic acid
B7VFD0, -
endogenous tannase, at 30C, pH 6.0
1019
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.5
-
Ag+
-
40C
0.49
-
Ba2+
-
40C
0.59
-
CaCl2
-
-
0.45
-
gallic acid
B7VFD0, -
endogenous tannase
0.57
-
Hg2+
-
40C
8.25
-
Zn2+
-
40C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.031
-
-
crude extract, pH 5.0, 45C
0.084
-
C7EDT0
crude extract, pH and temperature not specified in the publication
0.1602
-
-
6 step purified enzyme
0.18
-
-
purified enzyme, substrate catechin gallate
0.31
-
-
crude extract, at pH 5.5, 37C
0.43
-
-
purified enzyme, substrate epicatechin gallate
0.575
-
-
crude extract, pH 5.5, 50C
0.64
-
-
purified enzyme, substrate epigallocatechin gallate
0.84
-
-
purified enzyme, substrate tannic acid
0.87
-
-
purified enzyme, substrate gallic acid methyl ester
1.13
-
-
purified enzyme, substrate gallocatechin gallate
1.6
-
-
pH 6.0
1.91
-
-
after 61.61fold purification, pH 5.0, 45C
2.7
-
Hyalopus sp.
-
fermented broth, in 0.2 M acetate buffer, pH 5.5, at 40C, for 30 min
5.6
-
-
purified native enzyme
6.3
-
Hyalopus sp.
-
2.33fold purified enzyme, in 0.2 M acetate buffer, pH 5.5, at 40C, for 30 min
6.49
-
-
after 21fold purification, at pH 5.5, 37C
6.8
-
-
methyl gallate, activity increases 17fold, when the cells are grown in the presence of methyl gallate and 36fold in the presence of tannic acid
7.5
-
B3Y018
crude extract, at pH 8.0
13.63
-
C7EDT0
after 162fold purification, pH and temperature not specified in the publication
14.65
-
-
immobilized enzyme
15.27
-
-
crude filtrate, in 0.5 M citrate phosphate buffer, pH 5.0, at 50C
20
-
-
purified enzyme, in 100 mM sodium acetate, pH 5.0, 30% (w/v) diglyme, at 4C
22.85
-
-
after 39.74fold purification, pH 5.5, 50C
33.7
-
-
crude extract
61.8
-
-
purified isozyme TAH I
72.2
-
-
free enzyme
78
-
-
purified enzyme
82.2
-
-
purified isozyme TAH II
84.34
-
B3Y018
after 11.25fold purification, at pH 8.0
86
-
-
3 step purified enzyme
88.3
-
-
after 2.62fold purification
92
-
Candida sp.
-
8 step purified enzyme
118.8
-
-
3 step purification
135
-
-
5 step purification
170
-
-
purified enzyme
250
-
-
commercial preparation
264.5
-
-
enzyme produced under solid-state fermentation, after 153fold purification, at pH 7.0, 30C
355.6
-
-
purified enzyme
436.7
-
C7F6Y1
crude extract, pH 2.0, 30C
489
-
-
crude extract
2055
-
-
purified native enzyme
2055
-
-
after 135fold purification, in 0.5 M citrate phosphate buffer, pH 5.0, at 50C
2067
-
-
enzyme produced under submerged fermentation, after 477fold purification, at pH 7.0, 30C
2762
-
C7F6Y1
after 6.32fold purification, pH 2.0, 30C
9550
-
-
19.5fold purified enzyme
49330
-
-
pH 5.0, 30C
additional information
-
-
activity at different fermentation conditions, overview
additional information
-
-
-
additional information
-
-
activity depending on growth substrate
additional information
-
-
enzyme activity during cell growth in a fermenter culture, production kinetics
additional information
-
-
-
additional information
-
-
enzyme activity on different substrate for solid-state fermentation, overview
additional information
-
-
activity of extracellular and intracellular enzymes
additional information
-
-
-
additional information
-
-
paper chromatographic detection method development
additional information
-
-
the crude extract initially has a specific activity of 5.2 IU/mg. The 46fold purified enzyme has a specific activity of 238.14 IU/mg
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
-
-
two pH optima are observed for maximal activity, one at pH 2.0 and the other at a pH 8.0
2
-
C7F6Y1
two pH optima, pH 2.0 and pH 8.0, are recorded
4
5
-
native enzyme
4
-
-
a minor secondary peak at pH 4.0 is found in both enzymes produced under solid-state and submerged fermentations with 70% of tannase activation
4.3
5
-
potassium phosphate/citrate buffer
4.5
6.5
-
purified enzyme, 70% of maximal activity at pH 4.5, maximal activity at pH 5.5, and 80% of maximal activity at pH 6.5
4.5
-
-
optimal pH for enzyme production
4.5
-
-
immobilized enzyme
5
5.5
-
methyl gallate as substrate
5
6
-
submerged fermentation of Aspergillus
5
6.5
-
solid-state fermentation of Aspergillus
5
-
-
free enzyme
5
-
-
esterase activity
5
-
-
maximal cell growth and enzyme production in vivo
5
-
-
intra- and extracellular enzyme form
5
-
-
optimal pH for enzyme production
5
-
-
assay at
5
-
-
enzyme from co-culture with Rhizopus oryzae, the tannase needs an acidic environment to be active
5
-
-
enzyme from co-culture with Aspergillus foetidus, the tannase needs an acidic environment to be active
5
-
-
purified enzyme
5
-
-
intracellular enzyme of the submerged fermentation
5.5
6.5
-
liquid-surface fermentation of Aspergillus
5.5
-
-
assay at
5.5
-
-
strain PKL-104
5.5
-
-
isozymes TAH I and TAH II
5.5
-
-
at 37C
5.5
-
-
extracellular enzyme produced in the solid-state fermentation
5.5
-
-
free enzyme
5.7
6
B7VFD0, -
endogenous and recombinant tannases
5.8
-
-
maximal enzyme activity in vitro with partially purified enzyme
6
-
-
immobilized enzyme
6
-
-
optimal pH value for enzyme production in culture
6
-
-
tannase activity, strain N888
6
-
-
at 30C, using methyl 3,4,5-trihydroxybenzoate as substrate
6
-
-
maximum tannase activity at pH 6.0
6
-
-
at 30C
6
-
-
pH optimum for both enzymes produced under solid-state and submerged fermentations
6
-
Candida sp.
-
-
6
-
-
pI 4.3
6.4
-
E7D7J5
-
6.4
-
-
free enzyme
6.5
-
Hyalopus sp.
-
-
6.6
-
-
optimal growth and enzyme production pH value
7
-
-
purified recombinant tannase
7
-
-
immobilized enzyme
8
-
-
two pH optima are observed for maximal activity, one at pH 2.0 and the other at a pH 8.0
8
-
C7F6Y1
two pH optima, pH 2.0 and pH 8.0, are recorded
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1
7
C7F6Y1
the enzyme records a significant level of activity even at a pH of 1.0, however, the enzyme activity declines rapidly at pH 3.0-4.0 and is almost nil at a pH of 7.0
2
6
-
at pH 2.0 exhibits a relative activity of about 45%
2
8
-
extracellular enzyme produced in the solid-state fermentation
3
7
-
in vivo enzyme production
3
8
-
activity range
3
8
-
immobilized enzyme, pH 3: 40% of the maximal activity, pH 8: 20% of the maximal activity. Free enzyme, pH 3: 5% of the maximal activity, pH 8: 20% of the maximal activity
3
9
-
maximal activity at pH 5.0, 40% of maximal activity at pH 6.0
3.5
6
-
activity of the partially purified enzyme
3.5
6.5
-
cell growth and enzyme production in vivo
3.5
7
-
partially purified enzyme
4
5
-
tannase activity is greatly decreased below pH 4.0 and above pH 5.0
4
6.3
-
half-maximal acivities at pH 4.0 and pH 6.3, inactive below pH 3.8 and above pH 7.8
4
7
-
intracellular enzyme of the submerged fermentation
4.5
5.5
-
extracellular enzyme of the submerged fermentation
4.5
8
-
more than 50% activity between pH 4.5 and 8.0
5
7
-
pH 5.0 and pH 7.0: 50-60% of the maximal activity
5
8
-
more than 50% activity between pH 5.0 and 8.0
6
8
DEHEY7
highly active at pH 6.0-8.0. At pH 5.0, the enzyme shows less than 20% activity. At pH 3.0 and 10.0, the enzyme completely loses its activity
6
8
-
pH 6: 53% of the maximal activity, pH 8: 85% of the maximal activity
8
10
-
the enzyme shows more than 80% of activities at a pH range of 8.0-10.0
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
-
-
isozyme TAH II
20
-
-
isoform TAH II
25
-
-
isozyme TAH I
25
-
-
isoform TAH I
30
-
-
free enzyme; immobilized enzyme
30
-
-
optimal growth and enzyme production temperature
30
-
-
independent of fermentation process
30
-
-
in vivo assay at
30
-
C7F6Y1
-
30
-
-
free enzyme
35
40
B7VFD0, -
endogenous and recombinant tannases
35
40
-
activation energy of 24.3 kJ per mol
37
-
-
optimal temperature for enzyme production in culture, assay at
40
-
-
immobilized enzyme
40
-
-
assay at
40
-
-
in vitro assay at
40
-
-
enzyme from a commercial strain
40
-
-
enzyme from co-culture with Rhizopus oryzae
40
-
-
enzyme from co-culture with Aspergillus foetidus
40
-
-
purified recombinant tannase
40
-
-
maximum tannase activity at 40C
40
-
C7EDT0
-
40
-
-
free enzyme, activation energy 6.75 kcal per mol
45
-
-
immobilized enzyme
50
60
-
enzyme produced under submerged fermentation
50
-
-
assay at
50
-
-
extracellular enzyme form
50
-
-
assay at
50
-
Candida sp.
-
-
55
-
-
immobilized enzyme, activation energy 5.77 kcal per mol
60
-
-
strain PKL-104
60
-
-
intracellular enzyme form
60
-
Hyalopus sp.
-
-
60
-
-
with 0.1 M citrate buffer, pH 5.5
60
-
-
at a methyl gallate concentration of 4.4 mM
60
-
-
enzyme produced under solid-state fermentation
60
-
-
extracellular enzyme produced in the solid-state fermentation and in the submerged fermentation
70
-
-
strain N888
70
-
-
intracellular enzyme of the submerged fermentation
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
60
-
more than 50% activity between 4 and 60C
4
65
DEHEY7
active between 4 and 65C
5
80
C7F6Y1
the enzyme activity declines markedly along with increases in temperature above 60C
20
45
-
active between these temperatures
20
60
-
at 20C exhibits a relative activity of 10%
20
60
-
20C: 58% of the maximal activity, 60C: 2% of the maximal activity
20
70
-
maximal activity at 30C, 50% of maximal activity at 50C
20
80
-
purified enzyme, 20% of maximal activity at 20C and at 80C, 50% of maximal activity at 40C and at 65C, maximal activity at 50C
25
75
-
immobilized enzyme, 25C: 40% of the maximal activity, 75C: 18% of the maximal activity. Free enzyme, 25C: 57% of the maximal activity, 75C: 10% of the maximal activity
25
80
-
activity range
30
90
-
markedly reduced activity above 70C
additional information
-
-
temperature profile of the isozymes
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.8
-
-
strain N888
3.8
-
-
isoelectric focusing
4.4
-
C7F6Y1
isoelectric focusing
4.4
-
-
isoelectric focusing
5.1
-
DEHEY7
calculated from amino acid sequence
6.58
-
-
isoelectric focusing
9.9
-
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Lactobacillus plantarum CECT 748(T)
-
-
-
Manually annotated by BRENDA team
-
intracellular tannase is tightly bound with the mycelium
Manually annotated by BRENDA team
-
mycelium-bound tannase
Manually annotated by BRENDA team
-
submerged fermentation culture
Manually annotated by BRENDA team
Aspergillus niger MTCC 2425
-
-
-
Manually annotated by BRENDA team
additional information
-
culture conditions
Manually annotated by BRENDA team
additional information
-
culture methods, overview
Manually annotated by BRENDA team
additional information
-
modified solid-state fermentation
Manually annotated by BRENDA team
additional information
-
optimization of fermentation conditions, statistics
Manually annotated by BRENDA team
additional information
-
co-culture with Aspergillus foetidus, solid-state fermentation on tannin rich substrates
Manually annotated by BRENDA team
additional information
-
co-culture with Rhizopus oryzae, solid-state fermentation on tannin rich substrates
Manually annotated by BRENDA team
additional information
-
from solid-state culture, optimal on minimal medium containing 0.2% w/v glucose and 0.7% w/v tannic acid at 37 C and pH 6.0 in the absence of O2
Manually annotated by BRENDA team
additional information
-
from submerged or solid-state cultures with higher enzyme activity in the latter
Manually annotated by BRENDA team
additional information
-
optimization of culture conditions for tannase production, presence of additional carbon sources like maltose, glucose, and sucrose at 0.1% w/v concentration in the tannic acid medium is found to be effective for growth of the organism and inductive for enzyme production, though tannase synthesis is inhibited in maltose and arabinose, (NH4)2HPO4 as nitrogen source results in high enzyme production rates, overview
Manually annotated by BRENDA team
additional information
Aspergillus niger Aa-20
-
from submerged or solid-state cultures with higher enzyme activity in the latter
-
Manually annotated by BRENDA team
additional information
Rhizopus oryzae RO IIT RB-13
-
co-culture with Aspergillus foetidus, solid-state fermentation on tannin rich substrates
-
Manually annotated by BRENDA team
additional information
Aspergillus foetidus GMRB 013 MTCC 3557
-
co-culture with Rhizopus oryzae, solid-state fermentation on tannin rich substrates
-
Manually annotated by BRENDA team
additional information
Aureobasidium pullulans DBS66
-
optimization of culture conditions for tannase production, presence of additional carbon sources like maltose, glucose, and sucrose at 0.1% w/v concentration in the tannic acid medium is found to be effective for growth of the organism and inductive for enzyme production, though tannase synthesis is inhibited in maltose and arabinose, (NH4)2HPO4 as nitrogen source results in high enzyme production rates, overview
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Fusarium sp., Trichoderma sp.
-
-
-
Manually annotated by BRENDA team
-
5fold lower production level in culture than the intracellular form
-
Manually annotated by BRENDA team
Aspergillus heteromorphus MTCC 8818
-
-
-
-
Manually annotated by BRENDA team
Aspergillus aculeatus DBF 9
-
5fold lower production level in culture than the intracellular form
-
-
Manually annotated by BRENDA team
Bacillus licheniformis KBR 6, Aspergillus awamori BTMFW032, Lactobacillus sp. ASR-S1
-
-
-
-
Manually annotated by BRENDA team
-
5fold higher production level in culture than the extracellular form
Manually annotated by BRENDA team
-
intracellular tannase is tightly bound with the mycelium
Manually annotated by BRENDA team
Aspergillus aculeatus DBF 9
-
5fold higher production level in culture than the extracellular form
-
Manually annotated by BRENDA team
-
intracellular enzyme
Manually annotated by BRENDA team
-
mucous membrane
Manually annotated by BRENDA team
Aspergillus aculeatus DBF 9
-
intracellular enzyme
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45000
-
P78581
purified tannase with 2-mercaptoethanol, SDS-PAGE
50000
-
B3Y018
SDS-PAGE
50700
-
B3Y018
deduced from the amino acid sequence
55000
-
E7D7J5
gel filtration
61000
-
B7VFD0, -
mature enzyme, calculated from the ORF
100000
-
P78581
purified tannase without 2-mercaptoethanol, SDS-PAGE
149800
-
-
PAGE
150000
-
-
purified enzyme
150000
-
-
can exist as tetramer or dimer, gel fitration
155000
-
-
isozymes TAH I and TAH II, gel filtration
163000
-
-
gel filtration
186000
-
-
-
186000
-
-
glycoprotein containing 43% sugars, nondenaturing PAGE
192300
-
-
sedimentation equilibrium
194000
-
-
sedimentation diffusion
200000
-
-
gel filtration
230000
-
-
gel filtration
230000
-
C7F6Y1
gel filtration
250000
-
Candida sp.
-
gel filtration
290000
-
-
strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, sedimentation equilibrium
300000
-
-
can exist as tetramer or dimer, gel fitration
302000
-
-
gel filtration
310000
-
-
-
310000
-
-
gel filtration
310000
-
-
strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, gel filtration
320000
-
B7VFD0, -
native endogenous and recombinant tannases, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 90000, SDS-PAGE
?
-
x * 60000, SDS-PAGE
?
-
x * 50000, SDS-PAGE
?
-
x * 50000, SDS-PAGE
?
-
x * 50000, SDS-PAGE
?
-
x * 45000, SDS-PAGE
?
-
x * 55000, SDS-PAGE
?
-
x * 67000, SDS-PAGE
?
-
x * 59000, SDS-PAGE
?
-
x * 31000, SDS-PAGE
?
-
x * 46500, SDS-PAGE
?
-
x * 158000, SDS-PAGE
?
-
x * 85000, SDS-PAGE
?
-
x * 52000
?
-
x * 155000, SDS-PAGE
?
-
x * 102000, SDS-PAGE, x * 83000, SDS-PAGE
?
-
x * 80000-85000, strain N888
?
-
x * 90000, SDS-PAGE, x * 180000, SDS-PAGE
?
-
x * 100000, SDS-PAGE, x * 31000 + x * 34000, SDS-PAGE after treatment with N-glycosidase F, x * 90000, mass spectrometry
?
-
x * 168000, SDS-PAGE
?
-
x * 102000 + x * 83000
?
-
x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE
?
-
x * 87300, SDS-PAGE
?
-
x * 101000, SDS-PAGE
?
-
x * 102000, enzyme produced under solid-state fermentation, SDS-PAGE, x * 105000, enzyme produced under submerged fermentation, SDS-PAGE
?
-
x * 163000, SDS-PAGE
?
Hyalopus sp.
-
x * 000, SDS-PAGE
?
-
x * 218000, SDS-PAGE
?
-
x * 30000-33000, deglycosylated native enzyme, SDS-PAGE, x * 30000-34000, deglycosylated recombinant enzyme, SDS-PAGE
?
-
x * 302000, SDS-PAGE
?
-
x * 320000, SDS-PAGE
?
-
x * 45000-75000, recombinant enzyme, smeared bands on SDS-PAGE, x * 45000-80000, native enzyme, smeared bands on SDS-PAGE
?
-
x * 50700, SDS-PAGE
?
DEHEY7
x * 52980, calculated from amino acid sequence
?
Verticillium sp. P9
-
x * 155000, SDS-PAGE
-
?
Aspergillus niger ATTC 16620, Aspergillus niger GH1
-
x * 168000, SDS-PAGE
-
?
Lactobacillus pentosus 21A-3
-
x * 50000, SDS-PAGE
-
?
Aspergillus awamori MTCC9299
-
x * 101000, SDS-PAGE
-
?
Lactobacillus paraplantarum NSO120
-
x * 50000, SDS-PAGE
-
?
Aspergillus niger van Tieghem
-
x * 168000, SDS-PAGE
-
?
Lactobacillus plantarum ATCC 14917T
-
x * 67000, SDS-PAGE, x * 50000, SDS-PAGE
-
?
Lactobacillus plantarum ATCC 1491 T
-
x * 50000, SDS-PAGE
-
?
-
x * 52980, calculated from amino acid sequence
-
?
Aspergillus niger ITCC 6514.07
-
x * 168000, SDS-PAGE
-
?
Enterobacter sp. KPJ103
-
x * 90000, SDS-PAGE
-
?
Aspergillus niger MTCC 2425
-
x * 102000, SDS-PAGE, x * 83000, SDS-PAGE
-
dimer
-
2 * 53000, SDS-PAGE
dimer
Candida sp.
-
2 * 120000, SDS-PAGE
dimer
-
2 * 158000, SDS-PAGE
dimer
-
2 * 75000, SDS-PAGE, can exist as dimer or tetramer
dimer
Talaromyces variabilis IARI 2031
-
2 * 158000, SDS-PAGE
-
dimer
Aspergillus niger LCF 8
-
2 * 53000, SDS-PAGE
-
dimer
Candida sp. K-1
-
2 * 120000, SDS-PAGE
-
heterodimer
P78581
1 * 33000 + 1 * 30000, purified tannase digested with N-glycosidase F and loaded with 2-mercaptoethanol, SDS-PAGE
heterodimer
-
1 * 97000 + 1 * 43000, SDS-PAGE
heterodimer
Penicillium chrysogenum NCIM 923
-
1 * 97000 + 1 * 43000, SDS-PAGE
-
heterotetramer
-
2 * 45800 + 2 * 52000, SDS-PAGE
homodimer
-
2 * 158000, SDS-PAGE, 2 * 155000, gel filtration