Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM EDTA leads to 106% activity of the immobilized enzyme | uncultured bacterium | |
Urea | 1 mM urea leads to 106% and 113% activity for the free and immobilized enzyme, respectively | uncultured bacterium |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | uncultured bacterium |
General Stability | Organism |
---|---|
the immobilized enzyme retains its initial activity over 10 cycles and retains 80% of its initial activity after 26 cycles. After that, the activity of the immobilized enzyme is quickly lost, retaining about 40% of its initial activity after 46 cycles | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition of the free enzyme at 1 mM, the immobilized enzyme shows 93.7% residual activity at 1 mM | uncultured bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM Ca2+ leads to 126% and 113% activity for the free and immobilized enzyme, respectively | uncultured bacterium | |
Co2+ | 1 mM Co2+ leads to 105% activity of the immobilized enzyme | uncultured bacterium | |
Mg2+ | 1 mM Mg2+ leads to 120% and 110% activity for the free and immobilized enzyme, respectively | uncultured bacterium | |
Zn2+ | 1 mM Zn2+ leads to 108% activity of the immobilized enzyme | uncultured bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | uncultured bacterium | - |
gallate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA His-Bind resin column chromatography | uncultured bacterium |
Storage Stability | Organism |
---|---|
4°C, free enzyme in 100 mM phosphate buffer (pH 6.5), 16 days, complete loss of activity | uncultured bacterium |
4°C, immobilized enzyme in 100 mM phosphate buffer (pH 6.5), 30 days, less than 10% loss of activity | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propyl gallate + H2O | - |
uncultured bacterium | gallate + propanol | - |
? | |
tannic acid + H2O | - |
uncultured bacterium | gallate + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tan410 | - |
uncultured bacterium |
tannin acyl hydrolase | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
free enzyme | uncultured bacterium |
45 | - |
immobilized enzyme | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
the enzyme is the most active after incubating at 25°C for 12 h | uncultured bacterium |
50 | - |
the immobilized enzyme retains about 75% of its initial activity after incubation at 50°C for 4 h, whereas the free enzyme retains 50% of its initial activity when exposed to the same condition | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
free enzyme | uncultured bacterium |
7 | - |
immobilized enzyme | uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | more than 50% activity between pH 5.0 and 8.0 | uncultured bacterium |