Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain RIB40 | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
0.26 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
0.32 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
0.34 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
0.45 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
0.47 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
0.86 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
1.02 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
1.12 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
1.3 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
1.38 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
1.97 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Aspergillus oryzae | - |
gallate + D-glucose | - |
? | |
tannic acid + H2O | Aspergillus oryzae RIB 40 | - |
gallate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae RIB 40 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
TSKgel DEAE-5PW column chromatography and G3000-SWXL gel filtration | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechin gallate + H2O | - |
Aspergillus oryzae | gallate + catechin | - |
? | |
epicatechin gallate + H2O | best substrate | Aspergillus oryzae | gallate + epicatechin | - |
? | |
epicatechin gallate + H2O | best substrate | Aspergillus oryzae RIB 40 | gallate + epicatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Aspergillus oryzae | gallate + epigallocatechin | - |
? | |
ethyl gallate + H2O | worst substrate | Aspergillus oryzae | gallate + ethanol | - |
? | |
methyl gallate + H2O | - |
Aspergillus oryzae | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Aspergillus oryzae RIB 40 | gallate + methanol | - |
? | |
additional information | no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid | Aspergillus oryzae | ? | - |
? | |
additional information | no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid | Aspergillus oryzae RIB 40 | ? | - |
? | |
propyl gallate + H2O | - |
Aspergillus oryzae | gallate + propanol | - |
? | |
propyl gallate + H2O | - |
Aspergillus oryzae RIB 40 | gallate + propanol | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae | gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae RIB 40 | gallate + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000-33000, deglycosylated native enzyme, SDS-PAGE | Aspergillus oryzae |
? | x * 30000-34000, deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
? | x * 45000-75000, recombinant enzyme, smeared bands on SDS-PAGE | Aspergillus oryzae |
? | x * 45000-80000, native enzyme, smeared bands on SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
TanA | - |
Aspergillus oryzae |
tannin acyl hydrolase | - |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | the enzyme is stable up to 40°C. Native enzyme retains more than 70% of ist activity after incubation at 50°C for 60 min, whereas the residual activity of recombinant enzyme is approximately 40% after treatment at 50°C for 60 min | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.8 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
18.6 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
19 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
20.5 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
60.2 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
62.1 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
64.5 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
67.5 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
70.2 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
79.8 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
86.1 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
103.4 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | native enzyme | Aspergillus oryzae |
5 | - |
purified enzyme | Aspergillus oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.54 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
14.9 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
16.6 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
22.1 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
30.6 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
69.2 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
137.3 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
179.3 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
234 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
273.4 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
293.6 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
308.8 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae |