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Literature summary for 3.1.1.20 extracted from
- Production of tannase from wood-degrading fungus using as substrate plant residues: purification and characterization (2011), World J. Microbiol. Biotechnol., 27, 2325-2333.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.5 | - |
Tannic acid | at pH 5.5, 37°C | Trametes elegans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
3 * 50000, SDS-PAGE | Trametes elegans |
| 163000 | - |
gel filtration | Trametes elegans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes elegans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified by DEAE-Sepharose column chromatography (21times with 62.5% recovery) | Trametes elegans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.31 | - |
crude extract, at pH 5.5, 37°C | Trametes elegans |
| 6.49 | - |
after 21fold purification, at pH 5.5, 37°C | Trametes elegans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in 10 mM acetate buffer (pH 5.5), 3 months, no loss of activity | Trametes elegans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | - |
Trametes elegans | gallic acid + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 50000, SDS-PAGE | Trametes elegans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tannin acyl hydrolase | - |
Trametes elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trametes elegans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | the enzyme is stable at temperatures between 40 and 60°C but loses all activity at 90-100°C | Trametes elegans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
at 37°C | Trametes elegans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | - |
Trametes elegans |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Trametes elegans | Lenzites elegans reaches the highest tannase activity at 13 days of growth in media supplemented with glucose (1% w/v), tannic acid (0.5% w/v), and Fabiana densa powder (5% w/v) | up |
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