Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli JM 109 and expressed in Aspergillus oryzae strain AO1, a tannase low-producing and nitrate reductase-deficient strain | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE | Aspergillus oryzae |
33000 | - |
x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE | Aspergillus oryzae |
290000 | - |
strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, sedimentation equilibrium | Aspergillus oryzae |
310000 | - |
strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, gel filtration | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Aspergillus oryzae | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified from the strain AO1 | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | - |
Aspergillus oryzae | gallic acid + ? | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE | Aspergillus oryzae |