Any feedback?
Literature summary for 3.1.1.20 extracted from
- Extracellular tannase production by endophyitc Hyalopus sp (2009), J. Gen. Appl. Microbiol., 55, 255-259.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | in a short period of time the organism produces a large amount of tannase in an agricultural byproduct and cheap substrate like wheat bran, so in future, this organism can be applied for commercial tannase production. Broad range of pH and temperature stability of the enzyme can be exploited in various ways for pollution control in the leather industry and bioprocess industry in future | Hyalopus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyalopus sp. | - |
- |
- |
| Hyalopus sp. DSF3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified by acetone precipitation, 2.33fold with 79.06% recovery | Hyalopus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.7 | - |
fermented broth, in 0.2 M acetate buffer, pH 5.5, at 40°C, for 30 min | Hyalopus sp. |
| 6.3 | - |
2.33fold purified enzyme, in 0.2 M acetate buffer, pH 5.5, at 40°C, for 30 min | Hyalopus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | - |
Hyalopus sp. | gallic acid + D-glucose | - |
? |  |
| tannic acid + H2O | - |
Hyalopus sp. DSF3 | gallic acid + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tannase | - |
Hyalopus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Hyalopus sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | 60°C: heat stable for 10 min. 50°C: heat stable for 30 min, loses only 4% of its activity even after 60 min | Hyalopus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Hyalopus sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Hyalopus sp. |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Hyalopus sp. | enzyme production is depleted at higher tannic acid concentrations | down |
| Hyalopus sp. | organism in liquid submerged fermentation produces maximum extracellular tannase at the end of its exponential phase after 120 h. Wheat bran supports tannase production. 2.5% tannic acid is most suitable for maximum tannase synthesis through solid state fermentation | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
