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Literature summary for 3.1.1.20 extracted from
- Studies on tannin acyl hydrolase of microorganisms. Part III. Purification of the enzyme and some proporties of it (1968), Agric. Biol. Chem., 32, 803-809.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | activity is completely lost, when the enzyme is dialyzed against 0.025 M EDTA | Aspergillus oryzae |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)6Mo7O24 | 0.1 M, no effect on activity | Aspergillus oryzae | |
| BaCl2 | 0.1 M, no effect on activity | Aspergillus oryzae | |
| Ca2+ | CaCl2: 0.1 M, no effect on activity | Aspergillus oryzae | |
| Co2+ | 0.1 M, no effect on activity | Aspergillus oryzae | |
| Cu2+ | CuCl2: 70% inhibition, 0.1 M | Aspergillus oryzae | |
| FeCl3 | 0.1 M, no effect on activity | Aspergillus oryzae | |
| Mg2+ | MgCl2: no effect on activity | Aspergillus oryzae | |
| Mn2+ | 0.1 M, no effect on activity | Aspergillus oryzae | |
| additional information | no effect of the following metal ions at a concentration of 0.1 M: KCl, AlCl3, CdCl2 and SrCl2 | Aspergillus oryzae | |
| Ni2+ | NiSO4: 0.1 M, no effect on activity | Aspergillus oryzae | |
| Zn2+ | ZnCl2 70% inhibition, 0.1 M | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | Aspergillus oryzae | - |
? | - |
? | |
| tannic acid + H2O | Aspergillus oryzae No. 7 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
No. 7 | - |
| Aspergillus oryzae No. 7 | - |
No. 7 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 78 | - |
purified enzyme | Aspergillus oryzae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, citrate buffer, pH 5.5 or distilled water, as purified powder or as a solution, maintains activity over 6 months | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | - |
Aspergillus oryzae | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae No. 7 | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae No. 7 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Aspergillus oryzae |
| 55 | - |
activity completely lost after 20 min | Aspergillus oryzae |
| 85 | - |
activity completely lost after 10 min | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7.5 | stable for 12 h at 5°C | Aspergillus oryzae |
| 4.5 | 6 | stable for 25 h at 5°C | Aspergillus oryzae |
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