Information on EC 2.7.4.6 - nucleoside-diphosphate kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
2.7.4.6
-
RECOMMENDED NAME
GeneOntology No.
nucleoside-diphosphate kinase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors
-
-
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
mechanism
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
the catalytic mechanism involves a phosphoenzyme intermediate
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
the catalytic mechanism involves a phosphoenzyme intermediate
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
the catalytic mechanism involves a phosphoenzyme intermediate
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
the catalytic mechanism involves a phosphoenzyme intermediate
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
phosphoenzyme intermediate with 2-4 mol phosphate per mol of enzyme
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
mechanism
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
mechanism, role of 3-OH moiety of nucleotide
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
mechanism
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
overview on mechanism
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
two-step ping-pong mechanism
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
isozyme NDPKB shows a ping-pong phospho-transfer mechanism, salt and nucleotide dependent NDPKB assembly is dynamic and requires its catalytic activity
-
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
ping pong mechanism with formation of an enzyme intermediate phosphorylated at His118
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
ping pong mechanism with formation of an enzyme intermediate phosphorylated at His118
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenosine deoxyribonucleotides de novo biosynthesis
-
-
adenosine deoxyribonucleotides de novo biosynthesis II
-
-
CMP phosphorylation
-
-
guanosine deoxyribonucleotides de novo biosynthesis I
-
-
guanosine deoxyribonucleotides de novo biosynthesis II
-
-
guanosine ribonucleotides de novo biosynthesis
-
-
ppGpp biosynthesis
-
-
purine deoxyribonucleosides salvage
-
-
pyrimidine deoxyribonucleotide phosphorylation
-
-
pyrimidine deoxyribonucleotides biosynthesis from CTP
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis I
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis II
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis III
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis IV
-
-
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)
-
-
UTP and CTP de novo biosynthesis
-
-
non-pathway related
-
-
purine metabolism
-
-
pyrimidine metabolism
-
-
Purine metabolism
-
-
Pyrimidine metabolism
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
HsNDK
Halobacterium salinarum JMC 11081
-
-
kinase, nucleoside diphosphate (phosphorylating)
-
-
-
-
mitochondrial nucleoside diphosphate kinase
-
-
NDK
-
-
-
-
NDK
Halomonas sp. 4593, Halomonas sp. 593
-
-
-
NDK
Halomonas sp. 593
;
-
NDK
Leishmania amazonensis LV78
-
-
-
NDK
Mycobacterium smegmatis mc2155
-
-
-
NDK
Plasmodium falciparum FCR-3
-
-
-
NDP kinase
Pyrobaculum aerophilum DSM 7523
-
-
NDP kinase A
-
NDP kinase alpha
-
-
NDP kinase beta
-
-
NDPK
-
-
NDPK
-
-
NDPK IIpn
-
isoform
NDPK In
-
isoform
NDPK-D
-
-
NDPK-D
-
isoform
NDPK-D
-
mitochondrial isoform of nucleoside diphosphate kinase
NDPK1
-
isozyme
NDPK2
-
isozyme
NDPK2
isoform
NDPK3
-
isozyme
NM23 metastasis suppressor
-
exhibits Ndk activity
Nm23-H1
-
-
NM23-H4
-
-
nucleoside 5'-diphosphate kinase
-
-
-
-
nucleoside diphosphate (UDP) kinase
-
-
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
Halobacterium salinarum JMC 11081
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
Halomonas sp. 4593, Halomonas sp. 593
-
-
-
nucleoside diphosphate kinase
Halomonas sp. 593
;
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
Leishmania amazonensis LV78
-
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
Mycobacterium smegmatis mc2155
-
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
Plasmodium falciparum FCR-3
-
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphate kinase
-
nucleoside diphosphate kinase 1
-
nucleoside diphosphate kinase 1
-
nucleoside diphosphate kinase 2
-
nucleoside diphosphate kinase 2
-
nucleoside diphosphate kinase 2
-
nucleoside diphosphate kinase 2
-
nucleoside diphosphate kinase 3
-
nucleoside diphosphate kinase 3a
-
-
nucleoside diphosphate kinase A
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
-
nucleoside diphosphate kinase B
-
-
nucleoside diphosphate kinase B
-
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
nucleoside diphosphate kinase B
-
-
nucleoside diphosphate kinase B
-
-
nucleoside diphosphate kinase C
-
-
nucleoside diphosphate kinase C
-
-
-
nucleoside diphosphate kinase D
-
-
nucleoside diphosphate kinase-1
-
nucleoside diphosphate kinase-2
-
nucleoside diphosphate kinases alpha
-
-
nucleoside diphosphate kinases beta
-
-
nucleoside diphosphate phosphotransferase
-
-
nucleoside diphosphate phosphotransferase
Plasmodium falciparum FCR-3
-
-
-
nucleoside diphosphokinase
-
-
-
-
nucleotide diphosphate kinase
-
-
nucleotide phosphate kinase
-
-
-
-
UDP kinase
-
-
-
-
uridine diphosphate kinase
-
-
-
-
additional information
-
the enzyme belongs to the nucleoside diphosphate kinase family
additional information
-
the enzyme belongs to the nucleoside diphosphate kinase gene family
additional information
-
enzyme is able to specifically interact with proteins encoded by the bacteriophage T4
additional information
-
the enzyme belongs to the nucleoside diphosphate kinase family
additional information
the enzyme belongs to the nucleoside diphosphate kinase family
CAS REGISTRY NUMBER
COMMENTARY
9026-51-1
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Columbia-0, gene NDPK3a, isozyme NDPK3a
-
-
Manually annotated by BRENDA team
gene ndpk2
-
-
Manually annotated by BRENDA team
isozyme NDPK2
UniProt
Manually annotated by BRENDA team
several isozymes
-
-
Manually annotated by BRENDA team
oat, cv. Garry oats
-
-
Manually annotated by BRENDA team
calf
-
-
Manually annotated by BRENDA team
enzyme forms complexes with G protein beta gamma dimers and contributes to G protein activation
-
-
Manually annotated by BRENDA team
gene Cf-ndpk
UniProt
Manually annotated by BRENDA team
mitochondrial isoform
UniProt
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
gene ndk
-
-
Manually annotated by BRENDA team
infected and uninfected
-
-
Manually annotated by BRENDA team
infected by bacteriophage T4, host-coded enzyme from infected Escherichia coli is part of bacteriophage T4 dNTP-synthesizing multi-enzyme complex
-
-
Manually annotated by BRENDA team
overproducing strain JM83(pKT8P3)
-
-
Manually annotated by BRENDA team
strain B
-
-
Manually annotated by BRENDA team
wild type and enzyme deficient strains
-
-
Manually annotated by BRENDA team
isozyme NDK B; isozyme NDK B
Uniprot
Manually annotated by BRENDA team
Halobacterium salinarum 4R
strain 4R
-
-
Manually annotated by BRENDA team
Halobacterium salinarum JCM 11081
-
Uniprot
Manually annotated by BRENDA team
Halobacterium salinarum JMC 11081
-
Uniprot
Manually annotated by BRENDA team
strain 4593; strain 593, gene ndk
-
-
Manually annotated by BRENDA team
strain 593, gene ndk
SwissProt
Manually annotated by BRENDA team
Halomonas sp. 4593
strain 4593
-
-
Manually annotated by BRENDA team
Halomonas sp. 593
-
SwissProt
Manually annotated by BRENDA team
Halomonas sp. 593
strain 593, gene ndk
-
-
Manually annotated by BRENDA team
Halomonas sp. 593
strain 593, gene ndk
SwissProt
Manually annotated by BRENDA team
Jerusalem artichoke
-
-
Manually annotated by BRENDA team
eight NDPKB isozymes
UniProt
Manually annotated by BRENDA team
gene NME4 encoding isozyme NDPK-D
Uniprot
Manually annotated by BRENDA team
isoforms A and B
-
-
Manually annotated by BRENDA team
isozyme NDPKB
-
-
Manually annotated by BRENDA team
Impatiens holstii
-
-
-
Manually annotated by BRENDA team
strain LV78, clone 12-1, gene ndk
-
-
Manually annotated by BRENDA team
Leishmania amazonensis LV78
strain LV78, clone 12-1, gene ndk
-
-
Manually annotated by BRENDA team
expression in Escherichia coli
SwissProt
Manually annotated by BRENDA team
magellanicus, isozyme NDK B; novezelandiae, isozyme NDK B
-
-
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
UniProt
Manually annotated by BRENDA team
isozyme NDK B
-
-
Manually annotated by BRENDA team
Mycobacterium smegmatis mc2155
-
-
-
Manually annotated by BRENDA team
strain H37Ra
SwissProt
Manually annotated by BRENDA team
hagfish
-
-
Manually annotated by BRENDA team
haloalkaliphilic archaeon
-
-
Manually annotated by BRENDA team
haloalkaliphilic archaeon, enzyme binds specifically to natronobacterial flagellins
-
-
Manually annotated by BRENDA team
isoform Ndk-1
-
-
Manually annotated by BRENDA team
cv. Bright Yellow 2; study on mitochondria during programmed cell death
-
-
Manually annotated by BRENDA team
cultivar Kitaake
-
-
Manually annotated by BRENDA team
cv. Oregon sugarpod
-
-
Manually annotated by BRENDA team
isoform NDPK2; cultivar Alaska
UniProt
Manually annotated by BRENDA team
isoform NDPK3; cultivar Alaska
UniProt
Manually annotated by BRENDA team
isoform NDPK3; paraquat-resistant line R3-1
UniProt
Manually annotated by BRENDA team
isozyme NDPK3; recombinant isoform Ndpk3, enzyme additionally has nuclease activity cleaving supercoiled plasmid DNA, tRNAs or the 3UTR of atp9 mRNA
-
-
Manually annotated by BRENDA team
L. cv Oregon sugarpod
Uniprot
Manually annotated by BRENDA team
NDPK1
UniProt
Manually annotated by BRENDA team
NDPK2
UniProt
Manually annotated by BRENDA team
paraquat-resistant line R3-1
-
-
Manually annotated by BRENDA team
two mature isoforms of NDPK2 in plastids
-
-
Manually annotated by BRENDA team
strain FCR-3
-
-
Manually annotated by BRENDA team
Plasmodium falciparum FCR-3
strain FCR-3
-
-
Manually annotated by BRENDA team
strain 8822, kinase is associated in a complex with EC 6.2.1.5
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa 8822
strain 8822, kinase is associated in a complex with EC 6.2.1.5
-
-
Manually annotated by BRENDA team
strain ATCC 13525
-
-
Manually annotated by BRENDA team
; gene ndk
-
-
Manually annotated by BRENDA team
gene ndk
-
-
Manually annotated by BRENDA team
Pyrobaculum aerophilum DSM 7523
-
SwissProt
Manually annotated by BRENDA team
; isoform NDPK B
-
-
Manually annotated by BRENDA team
isoforms alpha, beta
-
-
Manually annotated by BRENDA team
isozyme NDPKB
-
-
Manually annotated by BRENDA team
isozymes NDP kinase alpha and NDP kinase beta
-
-
Manually annotated by BRENDA team
male Wistar rats, isozyme NDPK-D
-
-
Manually annotated by BRENDA team
Wistar strain
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar strain
-
-
Manually annotated by BRENDA team
brewer's yeast, Anheuser-Busch strain
-
-
Manually annotated by BRENDA team
baker's yeast, strain BJ2168
-
-
Manually annotated by BRENDA team
sugar cane
-
-
Manually annotated by BRENDA team
isoform NDPK1, strongly regulated by the NTP/NDP ratio
SwissProt
Manually annotated by BRENDA team
strain HB8
-
-
Manually annotated by BRENDA team
procyclic and bloodstream forms
-
-
Manually annotated by BRENDA team
multiple enzyme isoforms
UniProt
Manually annotated by BRENDA team
broad bean
-
-
Manually annotated by BRENDA team
female mature frog
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
loss of Nm23-H1 in diploid cells leads to cytokinetic furrow regression, followed by cytokinesis failure and generationof tetraploid cells. The loss of Nm23-H1, an event suspected to promote metastasis, additionally functions at an earlier stage of tumor development to drive the acquisition of chromosomal instability
physiological function
NDPK-D is proposed to couple ATP export through adenine nucleotide translocator to the synthesis of the other nucleoside triphosphates e.g. GTP
physiological function
-
isoform NDPK-D alters membrane organisation in terms of fluidity, hydration and lipid clusterin, mediates contact sites and contributes to the mitochondrial intermembrane space structuring
physiological function
there is a strong interaction between isoform NDPK2 and CAT1 in R3-1 plants, which possibly plays a vital role in the antioxidant defense against reactive oxygen species
physiological function
-
nucleoside diphosphate kinase exhibits GTPase activating protein activity towards Rab5 and Rab7. The enzyme is a putative virulence factor that inhibits phagosome maturation and promotes survival of mycobacteria within the macrophage
physiological function
-
the enzyme inhibits phagocytosis (but not amoeboid motility) and macropinocytosis which contrasts with its positive regulatory role in micropinocytosis. The enzyme has no effect on multicellular development and promotes growth in liquid
physiological function
-
nucleoside diphosphate kinase Nm23-H1 regulates chromosomal stability by activating the GTPase dynamin during cytokinesis
physiological function
key enzyme in maintaining cellular pools of all nucleoside triphosphates
physiological function
-
the enzyme inhibits phagocytosis (but not amoeboid motility) and macropinocytosis which contrasts with its positive regulatory role in micropinocytosis. The enzyme has no effect on multicellular development and promotes growth in liquid
-
physiological function
-
key enzyme in maintaining cellular pools of all nucleoside triphosphates
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-dehydro-3'-deoxyTDP + ATP
2',3'-dehydro-3'-deoxyTTP + ADP
show the reaction diagram
-
-
?
3'-fluoro-2',3'-dideoxyUDP + ATP
3'-fluoro-2',3'-dideoxyUTP + ADP
show the reaction diagram
better than 2,3-dideoxyUDP, but much poorer than 2-deoxyUDP
-
?
3'-fluoro-3'-deoxyTDP + ATP
3'-fluoro-3'-deoxyTTP + ADP
show the reaction diagram
-
-
?
ADP + CTP
ATP + CDP
show the reaction diagram
-
-
-
?
ADP + CTP
ATP + CDP
show the reaction diagram
-
-
-
?
ADP + CTP
ATP + CDP
show the reaction diagram
-
-
?
ADP + dCTP
ATP + dCDP
show the reaction diagram
-
-
-
?
ADP + dGTP
ATP + dGDP
show the reaction diagram
-
-
?
ADP + dTTP
ATP + dTDP
show the reaction diagram
-
-
-
?
ADP + dUTP
ATP + dUDP
show the reaction diagram
-
-
-
?
ADP + GTP
ATP + GDP
show the reaction diagram
-
-
-
?
ADP + GTP
ATP + GDP
show the reaction diagram
-
-
-
?
ADP + GTP
ATP + GDP
show the reaction diagram
-
-
?
ADP + GTP
ATP + GDP
show the reaction diagram
-
-
?
ADP + GTP
ATP + GDP
show the reaction diagram
-
-
-
?
ADP + TTP
ATP + TDP
show the reaction diagram
-
-
-
?
ADP + UTP
ATP + UDP
show the reaction diagram
-
-
-
?
ATP + 8-bromo-IDP
ADP + ?
show the reaction diagram
-
-
-
?
ATP + 8-bromo-IDP
ADP + ?
show the reaction diagram
-
-
-
?
ATP + 8-bromo-IDP
ADP + ?
show the reaction diagram
-
-
-
?
ATP + 8-bromoinosine 5'-diphosphate
ADP + 8-bromoinosine 5'-triphosphate
show the reaction diagram
-
-
-
?
ATP + adenosine 5'-methylene diphosphonate
ADP + ?
show the reaction diagram
-
-
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
-
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
-
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
-
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
best substrate
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
less effective dinucleotide substrate
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
reaction at 94% the rate of UDP
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
r
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
-
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
r
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
r
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
r
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
r
ATP + CDP
ADP + CTP
show the reaction diagram
-
-
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
highly effective dinucleotide substrate
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
reverse reaction at 21% the rate of ATP plus ADP
-
r
ATP + CDP
ADP + CTP
show the reaction diagram
-
reaction at 92% the rate of UDP
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
outer mitochondrial membrane-bound NDPK activity is involved in functional coupling to oxidative phosphorylation
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
Mycobacterium smegmatis mc2155
-
-
-
r
ATP + CDP
ADP + CTP
show the reaction diagram
Leishmania amazonensis LV78
-
-
-
r
ATP + dADP
ADP + dATP
show the reaction diagram
-
-
-
r
ATP + dADP
ADP + dATP
show the reaction diagram
-
less effective dinucleotide substrate
-
?
ATP + dADP
ADP + dATP
show the reaction diagram
-
reaction at 59% the rate of UDP
-
?
ATP + dCDP
ADP + dCTP
show the reaction diagram
-
-
-
r
ATP + dCDP
ADP + dCTP
show the reaction diagram
-
-
?
ATP + dCDP
ADP + dCTP
show the reaction diagram
-
-
-
r
ATP + dCDP
ADP + dCTP
show the reaction diagram
-
-
-
?
ATP + dCDP
ADP + dCTP
show the reaction diagram
-
-
r
ATP + dCDP
ADP + dCTP
show the reaction diagram
-
less effective dinucleotide substrate
-
?
ATP + dCDP
ADP + dCTP
show the reaction diagram
-
reaction at 61% the rate of UDP
-
?
ATP + dGDP
ADP + dGTP
show the reaction diagram
-
-
-
r
ATP + dGDP
ADP + dGTP
show the reaction diagram
-
-
-
r
ATP + dGDP
ADP + dGTP
show the reaction diagram
-
-
r
ATP + dGDP
ADP + dGTP
show the reaction diagram
-
less effective dinucleotide substrate
-
?
ATP + dGDP
ADP + dGTP
show the reaction diagram
-
reaction at 55% the rate of UDP
-
?
ATP + dGDP
ADP + dGTP
show the reaction diagram
dGDP is preferred over dTDP as an acceptor
-
?
ATP + dNDP
ADP + dNTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
?
ATP + dNDP
ADP + dNTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
?
ATP + dNDP
ADP + dNTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
-
r
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
-
r
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
highly effective dinucleotide substrate
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
in decreasing order of activity: dGDP, IDP, UDP, dUDP, CDP, GDP, dCDP
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
in decreasing order of activity: UDP, dUDP, dCDP, GDP, dGDP, CDP, GTP can fully replace ATP
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
reaction at 27% the rate of UDP
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
best substrate: dTDP
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
-
best substrate: dTDP
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
Plasmodium falciparum FCR-3
-
-
-
?
ATP + dUDP
?
show the reaction diagram
-
highly effective dinucleotide substrate
-
-
?
ATP + dUDP
?
show the reaction diagram
-
reaction at 39% the rate of UDP
-
-
?
ATP + dUDP
ADP + dUTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
an essential function of NDP kinase in ROS is phosphorylation of GDP to GTP, which is crucial for providing the functional cycle of transducin independently of its activation
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
assay by spontaneous release of GDP from G proteins. This GDP serves as a substrate for phosphotransfer by the NDPK, and the formed GTP then binds back to the G protein. NDPK is able to transfer phosphate also onto denatured proteins and thus also on the covalently linked GDP
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
-
assay by spontaneous release of GDP from G proteins. This GDP serves as a substrate for phosphotransfer by the NDPK, and the formed GTP then binds back to the G protein. NDPK is able to transfer phosphate also onto denatured proteins and thus also on the covalently linked GDP
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
Mycobacterium smegmatis mc2155
-
-
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
Plasmodium falciparum FCR-3
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
Leishmania amazonensis LV78
-
-
-
r
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
r
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
r
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
-
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all nucleoside triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, involved in coleoptile elongation and anaerobic stress response
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, broad substrate specificity, in plants involved in different signaling pathways
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, enzyme is able to specifically interact with proteins encoded by the bacteriophage T4
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, NM23 metastasis suppressor involved in inactivation of oncogenic forms of Ras proteins
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
MgATP promotes correct folding and association
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Mycobacterium smegmatis mc2155
-
-
-
r
ATP + NDP
ADP + NTP
show the reaction diagram
Plasmodium falciparum FCR-3
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Halobacterium salinarum 4R
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages
-
?
ATP + nucleoside diphosphate
ADP + nucleoside triphosphate
show the reaction diagram
-
-
?
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
-
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
?
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
?
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
?
ATP + TDP
ADP + TTP
show the reaction diagram
the enzyme activity depends largely upon the membrane-bound state
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
the enzyme activity depends largely upon the membrane-bound state
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
Halomonas sp. 593
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
Halomonas sp. 593
-
-
?
ATP + tubulin-GDP
ADP + tubulin-GTP
show the reaction diagram
-
-
-
?
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
-
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
-
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
?
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
?
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
r
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
r
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
?
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
r
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
-
?
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
?
ATP + UDP
ADP + UTP
show the reaction diagram
-
-
r
ATP + UDP
ADP + UTP
show the reaction diagram
-
highly effective dinucleotide substrate
-
-
ATP + UDP
ADP + UTP
show the reaction diagram
-
best dinucleotide substrate
-
r
ATP + UDP
ADP + UTP
show the reaction diagram
-
reverse reaction at 32% the rate of the reaction ATP plus ADP
-
r
ATP + UDP
ADP + UTP
show the reaction diagram
NDPK regulates a variety of eukaryotic cellular activities including cell proliferation, development, and differentiation
-
r
ATP + UDP
ADP + UTP
show the reaction diagram
Leishmania amazonensis LV78
-
-
-
r
ATP + XDP
ADP + XTP
show the reaction diagram
-
reaction at 37% the rate of UDP
-
?
CTP + ADP
CDP + ATP
show the reaction diagram
-
-
?
CTP + CDP
CDP + CTP
show the reaction diagram
-
-
-
?
CTP + CDP
CDP + CTP
show the reaction diagram
-
reaction at 15% the rate of ATP
-
?
dATP + ADP
dADP + ATP
show the reaction diagram
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
at 25C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25C and 50C
-
?
dATP + CDP
dADP + CTP
show the reaction diagram
-
-
-
r
dATP + GDP
dADP + GTP
show the reaction diagram
-
-
-
?
dATP + IDP
dADP + ITP
show the reaction diagram
-
-
-
?
dATP + UDP
dADP + UTP
show the reaction diagram
-
-
-
?
dCTP + ADP
dCDP + ATP
show the reaction diagram
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
at 25C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25C and 50C
-
?
dGDP + ATP
dGTP + ADP
show the reaction diagram
-
-
-
?
dGTP + ADP
dGDP + ATP
show the reaction diagram
-
-
-
?
dGTP + ADP
dGDP + ATP
show the reaction diagram
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66100 and 156165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor
-
?
dTTP + ADP
dTDP + ATP
show the reaction diagram
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
at 25C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25C and 50C
-
?
gamma-S-ATP + GDP
ADP + gamma-S-GTP
show the reaction diagram
-
poor substrate
-
?
gamma-S-ATP + GDP
ADP + gamma-S-GTP
show the reaction diagram
-
poor substrate
-
?
gamma-S-ATP + GDP
ADP + gamma-S-GTP
show the reaction diagram
-
poor substrate
-
?
gamma-S-GTP + GDP
GDP + gamma-S-GTP
show the reaction diagram
-
poor substrate
-
?
gamma-S-GTP + GDP
GDP + gamma-S-GTP
show the reaction diagram
-
poor substrate
-
?
gamma-S-GTP + GDP
GDP + gamma-S-GTP
show the reaction diagram
-
poor substrate
-
?
GDP + ATP
GTP + ADP
show the reaction diagram
-
-
?
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
?
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
-
-
?
GTP + ADP
GDP + ATP
show the reaction diagram
-
reaction at 12% the rate of ATP
-
-
GTP + ADP
GDP + ATP
show the reaction diagram
-
GDP is less effective dinucleotide substrate
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
dADP can replace ADP
-
r
GTP + ADP
GDP + ATP
show the reaction diagram
-
reverse reaction at 62% the rate of UDP
-
r
GTP + CDP
GDP + CTP
show the reaction diagram
-
-
-
?
GTP + CDP
GDP + CTP
show the reaction diagram
-
-
-
r
GTP + dGDP
GDP + dGTP
show the reaction diagram
-
-
-
-
GTP + dTDP
GDP + dTTP
show the reaction diagram
-
-
-
?
GTP + GDP
GDP + GTP
show the reaction diagram
-
-
-
?
GTP + GDP
GDP + GTP
show the reaction diagram
-
in decreasing order of activity: UTP, dGTP, dTTP, CTP, dCTP, dUTP, equally active as ATP
-
?
GTP + nucleoside diphosphate
GDP + nucleoside triphosphate
show the reaction diagram
Pseudomonas sp., Halomonas sp., Halomonas sp. 593, Halomonas sp. 4593
-
-
-
?
GTP + UDP
GDP + UTP
show the reaction diagram
-
-
-
?
GTP + UDP
GDP + UTP
show the reaction diagram
-
-
-
?
GTP-bound Ras protein + NDP
GDP-bound Ras protein + NTP
show the reaction diagram
-
in vitro, inactivation of mutant oncogenic Ras proteins
-
?
ITP + ADP
IDP + ATP
show the reaction diagram
-
-
-
?
ITP + ADP
IDP + ATP
show the reaction diagram
-
-
-
?
ITP + ADP
IDP + ATP
show the reaction diagram
-
reverse reaction at 42% the rate of UDP
-
?
NDP + NTP
NTP + NDP
show the reaction diagram
-
-
-
r
NDP + NTP
NTP + NDP
show the reaction diagram
-
-
-
r
NDP + NTP
NTP + NDP
show the reaction diagram
-
autophosphorylation and phosphorylation of histone H1
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
may be involved in regulation of growth, development and signal-transduction processes
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
major component of nucleoside triphosphate synthetic pathway
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
enzyme forms complexes with G protein beta gamma dimers and contributes to G protein activation
-
r
NDP + NTP
NTP + NDP
show the reaction diagram
-
involved in RNA- and DNA metabolism
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
overview on roles in metabolic pathways and nucleic acid synthesis
-
-
NTP + ADP
NDP + ATP
show the reaction diagram
-
-
?
NTP + NDP
NDP + NTP
show the reaction diagram
NDPK is a key metabolic enzyme that catalyzes the synthesis of non-adenine nucleoside triphosphate by transferring the terminal phosphate between NDP and NTP
-
r
NTP + NDP
NDP + NTP
show the reaction diagram
the high-energy phosphate is mainly supplied by ATP, but the enzymes have broad substrate specificity and use the ribose and deoxyribose forms of both, purine and pyrimidine nucleotides, ping-pong mechanism with a phosphoenzyme/phosphohistidine intermediary involved in the transfer reaction, overview
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
no substrates are several nucleoside monophosphates
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
substrate 8-aza-UDP, human erythrocytic pI-variant 5.8
-
r
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
in decreasing order of activity: UTP, dTTP, CTP, dATP, ATP, dGTP, GTP
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
the nature of the nucleoside triphosphate controls the rate of the reaction
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
Escherichia coli 201
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
Pseudomonas aeruginosa 8822
-
-
-
?
TTP + ADP
TDP + ATP
show the reaction diagram
-
-
?
tubulin-GDP-complex + ATP
tubulin-GTP-complex + ADP
show the reaction diagram
-
-
-
?
UTP + ADP
UDP + ATP
show the reaction diagram
-
-
?
UTP + CDP
UDP + CTP
show the reaction diagram
-
-
-
-
UTP + CDP
UDP + CTP
show the reaction diagram
-
best substrate: UTP, in decreasing order of activity: dATP, ITP, dTTP, ATP, dGTP, GTP, dCTP, CTP
-
-
UTP + dCDP
UDP + dCTP
show the reaction diagram
-
-
-
?
UTP + dCDP
UDP + dCTP
show the reaction diagram
-
best substrate: UTP, in decreasing order of activity: ITP, dATP, dTTP, dGTP, GTP, ATP, CTP, dCTP
-
?
UTP + dTDP
UDP + dTDP
show the reaction diagram
-
best substrates, nucleoside triphosphates in decreasing order of activity: dTTP, CTP, dCTP, dATP, dGTP, GTP
-
?
UTP + dTDP
UDP + dTDP
show the reaction diagram
-
best substrate: UTP, in decreasing order of activity: ITP, dATP, dTTP, GTP, ATP, dGTP, dUTP, CTP, dCTP
-
?
UTP + dTDP
UDP + dTDP
show the reaction diagram
-
nucleoside diphosphates in decreasing order of activity: CDP, UDP, GDP, dGDP, dCDP, dADP, ADP
-
?
UTP + UDP
UDP + UTP
show the reaction diagram
-
reaction at 31% the rate of ATP
-
?
ITP + UDP
IDP + UTP
show the reaction diagram
-
-
-
?
additional information
?
-
-
autophosphorylation and phosphorylation of histone H1
-
-
-
additional information
?
-
-
strong preference for D-enantiomers of antiviral nucleotide analogs like ddATP, ddCTP, 3'-deoxy-3'-thymidine, 2',3'-didehydro-2',3'-dideoxythymidine
-
-
-
additional information
?
-
-
in the absence of nucleoside diphosphates the enzyme undergoes Mg2+-dependent stoichiometric autophosphorylation using ATP, GTP or gamma-thiotriphosphate as phosphate donor, 2 mol phosphate per mol enzyme
-
-
-
additional information
?
-
catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments
-
-
-
additional information
?
-
-
enzyme is involved in receptor-independent regulation of cAMP synthesis and contractility in intact cardiomyocytes and has histidine kinase activity for phosphorylation of G protein subunit beta phosphorylation at H266
-
-
-
additional information
?
-
-
role of enzyme in dUTP detoxification to compensate for the absence of a dUTPase in Mimivirus
-
-
-
additional information
?
-
-
enzyme additionally has nuclease activity cleaving supercoiled plasmid DNA, tRNAs or the 3UTR of atp9 mRNA. Nuclease- and kinase mechanisms are separate
-
-
-
additional information
?
-
-
preferential affinity for deoxypyrimidine nucleotides
-
-
-
additional information
?
-
-
critical role of the two amino acid residues, E134 and E135, at the carboxy-terminal region of NDK in determining the halophilic characteristics of the protein, overview
-
-
-
additional information
?
-
-
In addition to the receptor-induced GDP/GTP exchange, G protein alpha subunits can be activated by a phosphate transfer via a plasma membrane-associated complex of NDPK B and G protein betagamma-dimers, Gbetagamma, requiring the intermediate phosphorylation of His266, the histidine kinase activity of NDPK B for Gbeta is important for the receptor-independent regulation of cAMP synthesis and contractility in intact cardiomyocytes, overview
-
-
-
additional information
?
-
key enzyme involved in the homeostasis of nucleoside triphosphates
-
-
-
additional information
?
-
kinetic parameters and regulation mechanisms of NDPK1 and NDPK2, overview
-
-
-
additional information
?
-
-
MDA-MB-435S cell-secreted NDPK-B supports tumour formation by modulating ATP levels locally to activate endothelial cell P2Y receptor-mediated angiogenesis, overview
-
-
-
additional information
?
-
-
NdK is a stable enzyme capable of decreasing extracellular ATP levels
-
-
-
additional information
?
-
NDK-1 plays an important role in protecting conidial viability under light stress in Neurospora crassa
-
-
-
additional information
?
-
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
-
additional information
?
-
-
NDPK2 is a H2O2 signaling protein that performs autophosphorylation. NDPK2 and H2O2 are involved in salt resistance, overview
-
-
-
additional information
?
-
NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate
-
-
-
additional information
?
-
nucleoside diphosphate kinase is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except ATP, but it also plays a significant role in signal transduction pathways involved in oxidative stress. The enzyme protects the plants against reactive ocygen species, heat, and salt stress, overview
-
-
-
additional information
?
-
-
nucleoside diphosphate kinase is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions, differentially for adenylate kinase isozymes, overview
-
-
-
additional information
?
-
-
the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate, NTP, to a nucleoside diphosphate, NDP
-
-
-
additional information
?
-
-
the enzyme is involved in endoplasmic reticulum membranes structure formation, the mechanism of membrane extension involves direct lipid binding, as NDKB-bound phosphatidylinositol-4-phosphate, phosphatidylinositol-4,5-diphosphate, and phosphatidic acid. Binding to these anionic lipids requires clusters of basic residues on the surface of the NDKB hexamer. Amino acid changes in NDKB, that block lipid binding, also block endoplasmic reticulum network extension, overview
-
-
-
additional information
?
-
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
-
additional information
?
-
the transfer of high energetic phosphate by NDPK and/or the beta subunit of G proteins can induce G protein activation. Interaction of nucleoside diphosphate kinase B with heterotrimeric G protein betagamma dimers influencing the G protein activation and stability, mechanism, detailed overview
-
-
-
additional information
?
-
-
the transfer of high energetic phosphate by NDPK and/or the beta subunit of G proteins can induce G protein activation. Interaction of nucleoside diphosphate kinase B with heterotrimeric G protein betagamma dimers influencing the G protein activation and stability, mechanism, detailed overview
-
-
-
additional information
?
-
-
both recombinant and native Leishmania NdK utilized ATP to produce expected nucleoside triphosphates in the presence of nucleoside diphosphates in excess
-
-
-
additional information
?
-
-
coupling of the formation of ATP to phenazine methosulfate and iodonitrotetrazolium chloride, coupling is achieved using hexokinase and G6PDH, which produce the necessary NADPH molecules for formazan precipitation at the site of enzyme activity, method optimization, overview, measurement of the reverse enzyme reactions, overview
-
-
-
additional information
?
-
-
interaction of recombinant NDP kinases alpha and beta and their mutant chimeric derivatives with rhodopsin-transducin complex, binding to ROS photoreceptor membranes, overview
-
-
-
additional information
?
-
-
NDKB stabilizes an extended endoplasmic reticulum network in vitro, overview
-
-
-
additional information
?
-
NDPK B acts as histidine kinase in a NDPK B/Gbetagamma complex, NDPK B/Gbetagamma complex formation, structure, and mode of action, overview. Nucleoside diphosphate kinase is a ubiquitous enzyme that catalyses the transfer of the gamma-phosphate from nucleoside 5'-triphosphates to nucleoside 5'-diphosphates by a ping-pong mechanism involving the formation of a high energy phosphate intermediate on His118
-
-
-
additional information
?
-
-
NDPK B acts as histidine kinase in a NDPK B/Gbetagamma complex, NDPK B/Gbetagamma complex formation, structure, and mode of action, overview. Nucleoside diphosphate kinase is a ubiquitous enzyme that catalyses the transfer of the gamma-phosphate from nucleoside 5'-triphosphates to nucleoside 5'-diphosphates by a ping-pong mechanism involving the formation of a high energy phosphate intermediate on His118
-
-
-
additional information
?
-
-
NDPK catalyzes the transfer of gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates
-
-
-
additional information
?
-
NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
-
additional information
?
-
-
NDPK3 protein additionally cleaves not only supercoiled plasmid DNA, but also highly structured RNA molecules such as tRNAs or the 3'UTR of the atp9 mRNA suggesting that the NDPK3 nuclease activity has a structural requirement, ATP inhibits this nuclease activity, while ADP has no effect, the nuclease- and the kinase-mechanisms are separate, overview
-
-
-
additional information
?
-
-
protein-protein interaction analysis of adenylate kinases from Arabidopsis thaliana with the immobilized recombinant His-tagged NDPK from Pisum sativum, the interaction influences both enzyme activities, overview
-
-
-
additional information
?
-
substrate specificities of wild-type and G114R mutant enzymes, overview
-
-
-
additional information
?
-
-
NDPK co-immunoprecipitates reciprocally with AMP-activated kinase and is co-localised with this enzyme in human airways
-
-
-
additional information
?
-
NDPK1 exhibits nuclease activity cleaving not only linear DNA, but also supercoiled plasmid DNA, ATP or ADP do not affect the nuclease activity while the absence of Mg2+ completely inhibits this activity
-
-
-
additional information
?
-
-
isoform NDPK-D is able to bind in a non-negligible manner to zwitterionic phosphatidylcholine
-
-
-
additional information
?
-
-
the binding affinity of different nucleotides with Plasmodium falciparum nucleoside diphosphate kinase is varied according to the following order ADP/GDP > dGDP > dADP > dTDP > CDP > dCDP >U DP. The enzyme shows more preferences to ribonucleotides over deoxyribonucleotides
-
-
-
additional information
?
-
Leishmania amazonensis LV78
-
NdK is a stable enzyme capable of decreasing extracellular ATP levels, both recombinant and native Leishmania NdK utilized ATP to produce expected nucleoside triphosphates in the presence of nucleoside diphosphates in excess
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + CDP
ADP + CTP
show the reaction diagram
-
outer mitochondrial membrane-bound NDPK activity is involved in functional coupling to oxidative phosphorylation
-
?
ATP + dCDP
ADP + dCTP
show the reaction diagram
Q9UUY8
-
-
r
ATP + dGDP
ADP + dGTP
show the reaction diagram
Q9UUY8
-
-
r
ATP + dNDP
ADP + dNTP
show the reaction diagram
A5U5E1
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
?
ATP + dNDP
ADP + dNTP
show the reaction diagram
P9WJH7
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
?
ATP + dNDP
ADP + dNTP
show the reaction diagram
Q07661
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
?
ATP + dTDP
ADP + dTTP
show the reaction diagram
Q4E256
-
-
r
ATP + dTDP
ADP + dTTP
show the reaction diagram
Q9UUY8
-
-
r
ATP + GDP
ADP + GTP
show the reaction diagram
-
-
-
?
ATP + GDP
ADP + GTP
show the reaction diagram
-
an essential function of NDP kinase in ROS is phosphorylation of GDP to GTP, which is crucial for providing the functional cycle of transducin independently of its activation
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
r
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
r
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
-
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Q4E256, Q4E4R9
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Q401C5
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Q5UQL3
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
A5U5E1
contributes to the maintenance of the cellular pools of all nucleoside triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
P9WJH7
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Q07661
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, involved in coleoptile elongation and anaerobic stress response
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Q8LP25, Q8RUX2, Q8S945
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Q9SP13
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, broad substrate specificity, in plants involved in different signaling pathways
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, enzyme is able to specifically interact with proteins encoded by the bacteriophage T4
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, NM23 metastasis suppressor involved in inactivation of oncogenic forms of Ras proteins
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
P15531
MgATP promotes correct folding and association
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Mycobacterium smegmatis mc2155
-
-
-
r
ATP + NDP
ADP + NTP
show the reaction diagram
Plasmodium falciparum FCR-3
-
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
Halobacterium salinarum 4R
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages
-
?
ATP + TDP
ADP + TTP
show the reaction diagram
O00746
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
-
-
-
r
ATP + TDP
ADP + TTP
show the reaction diagram
Halomonas sp., Halomonas sp. 593
Q83WH5
-
-
r
NDP + NTP
NTP + NDP
show the reaction diagram
-
-
-
r
NDP + NTP
NTP + NDP
show the reaction diagram
-
-
-
r
NDP + NTP
NTP + NDP
show the reaction diagram
-
autophosphorylation and phosphorylation of histone H1
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
may be involved in regulation of growth, development and signal-transduction processes
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
major component of nucleoside triphosphate synthetic pathway
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
enzyme forms complexes with G protein beta gamma dimers and contributes to G protein activation
-
r
NDP + NTP
NTP + NDP
show the reaction diagram
-
involved in RNA- and DNA metabolism
-
-
NDP + NTP
NTP + NDP
show the reaction diagram
-
overview on roles in metabolic pathways and nucleic acid synthesis
-
-
NTP + ADP
NDP + ATP
show the reaction diagram
P61136
-
-
?
NTP + NDP
NDP + NTP
show the reaction diagram
Q4E256
-
-
r
NTP + NDP
NDP + NTP
show the reaction diagram
A2TLM3
NDPK is a key metabolic enzyme that catalyzes the synthesis of non-adenine nucleoside triphosphate by transferring the terminal phosphate between NDP and NTP
-
r
tubulin-GDP-complex + ATP
tubulin-GTP-complex + ADP
show the reaction diagram
-
-
-
?
ATP + UDP
ADP + UTP
show the reaction diagram
A2TLM3
NDPK regulates a variety of eukaryotic cellular activities including cell proliferation, development, and differentiation
-
r
additional information
?
-
P9WJH7
catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments
-
-
-
additional information
?
-
-
enzyme is involved in receptor-independent regulation of cAMP synthesis and contractility in intact cardiomyocytes and has histidine kinase activity for phosphorylation of G protein subunit beta phosphorylation at H266
-
-
-
additional information
?
-
-
role of enzyme in dUTP detoxification to compensate for the absence of a dUTPase in Mimivirus
-
-
-
additional information
?
-
-
critical role of the two amino acid residues, E134 and E135, at the carboxy-terminal region of NDK in determining the halophilic characteristics of the protein, overview
-
-
-
additional information
?
-
-
In addition to the receptor-induced GDP/GTP exchange, G protein alpha subunits can be activated by a phosphate transfer via a plasma membrane-associated complex of NDPK B and G protein betagamma-dimers, Gbetagamma, requiring the intermediate phosphorylation of His266, the histidine kinase activity of NDPK B for Gbeta is important for the receptor-independent regulation of cAMP synthesis and contractility in intact cardiomyocytes, overview
-
-
-
additional information
?
-
P47923
key enzyme involved in the homeostasis of nucleoside triphosphates
-
-
-
additional information
?
-
Q4E256
kinetic parameters and regulation mechanisms of NDPK1 and NDPK2, overview
-
-
-
additional information
?
-
-
MDA-MB-435S cell-secreted NDPK-B supports tumour formation by modulating ATP levels locally to activate endothelial cell P2Y receptor-mediated angiogenesis, overview
-
-
-
additional information
?
-
-
NdK is a stable enzyme capable of decreasing extracellular ATP levels
-
-
-
additional information
?
-
Q9UUY8
NDK-1 plays an important role in protecting conidial viability under light stress in Neurospora crassa
-
-
-
additional information
?
-
O64903
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
-
additional information
?
-
-
NDPK2 is a H2O2 signaling protein that performs autophosphorylation. NDPK2 and H2O2 are involved in salt resistance, overview
-
-
-
additional information
?
-
P15531
NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate
-
-
-
additional information
?
-
O64903
nucleoside diphosphate kinase is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except ATP, but it also plays a significant role in signal transduction pathways involved in oxidative stress. The enzyme protects the plants against reactive ocygen species, heat, and salt stress, overview
-
-
-
additional information
?
-
-
nucleoside diphosphate kinase is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions, differentially for adenylate kinase isozymes, overview
-
-
-
additional information
?
-
-
the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate, NTP, to a nucleoside diphosphate, NDP
-
-
-
additional information
?
-
-
the enzyme is involved in endoplasmic reticulum membranes structure formation, the mechanism of membrane extension involves direct lipid binding, as NDKB-bound phosphatidylinositol-4-phosphate, phosphatidylinositol-4,5-diphosphate, and phosphatidic acid. Binding to these anionic lipids requires clusters of basic residues on the surface of the NDKB hexamer. Amino acid changes in NDKB, that block lipid binding, also block endoplasmic reticulum network extension, overview
-
-
-
additional information
?
-
Q81SV8
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
-
additional information
?
-
P15531
the transfer of high energetic phosphate by NDPK and/or the beta subunit of G proteins can induce G protein activation. Interaction of nucleoside diphosphate kinase B with heterotrimeric G protein betagamma dimers influencing the G protein activation and stability, mechanism, detailed overview
-
-
-
additional information
?
-
-
the transfer of high energetic phosphate by NDPK and/or the beta subunit of G proteins can induce G protein activation. Interaction of nucleoside diphosphate kinase B with heterotrimeric G protein betagamma dimers influencing the G protein activation and stability, mechanism, detailed overview
-
-
-
additional information
?
-
-
NDPK co-immunoprecipitates reciprocally with AMP-activated kinase and is co-localised with this enzyme in human airways
-
-
-
additional information
?
-
Leishmania amazonensis LV78
-
NdK is a stable enzyme capable of decreasing extracellular ATP levels
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Ca2+
-
requirement, about half as effective as Mg2+, Mn2+ or Co2+, pI 7.3-enzyme variant
Ca2+
-
can replace Mg2+, about 20% as effective, inhibitory above 5 mM
Co2+
-
in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+; requirement
Co2+
-
equally effective as Mg2+ or Mn2+, pI 7.3-enzyme variant; requirement
Fe2+
-
requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
KCl
-
concentration of 3 M required for activity
Mg2+
-
requirement; true substrates: Mg-ADP and Mg-ATP
Mg2+
-
in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+; requirement
Mg2+
-
probably tightly bound, 30% of activity even after prolonged exposure to EDTA
Mg2+
-
requirement
Mg2+
-
requirement; true substrates: Mg2+-nucleotide complexes
Mg2+
-
equally effective as Mn2+ or Co2+, pI 7.3-enzyme variant
Mg2+
-
true substrates: Mg2+-nucleotide complexes
Mg2+
-
true substrates: Mg2+-nucleotide complexes
Mg2+
-
requirement
Mg2+
-
2mM, stimulation up to 7-fold
Mg2+
-
requirement, inhibitory above 5 mM
Mg2+
-
required for DNA cleavage reaction
Mg2+
divalent cation required for catalysis, Mg2+ preferred over other ions
Mg2+
MgATP promotes correct folding and association
Mg2+
-
-
Mg2+
-
the presence of Mg2+ enhances enthalpy changes by stabilizing water molecule hydrogen bonding network, resulting in favorable enthalpy changes, the absence of Mg2+ induces a noticeable loss in binding enthalpies and positive favorable entropic changes in GDP binding, indicating a more unstable enzyme-ligand complex formation and lesser complex tightening
Mg2+
-
required for activity, with no exogenous Mg2+ added, but in the absence of EDTA at low temperature the activity of membrane-bound NDPK is below the limit of detection
Mg2+
-
the activity is dependent on a metal ion (mainly Mg2+)
Mn2+
-
in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+; requirement
Mn2+
-
equally effective as Co2+, pI 7.3-enzyme variant; equally effective as Mg2+; requirement
Na+
-
optimal NaCl concentration is 1 M
Na+
optimal NaCl concentration is 2 M
NaCl
-
concentration of 2 M required for activity
NaCl
-
nucleoside diphosphate kinase prepared from Halobacterium salinarum is active and stable in the absence of salt. Recombinant nucleoside diphosphate kinase expressed in Escherichia coli requires salt for activation in vitro, but once it acquires the proper folding, it no longer requires the presence of salts for its activity and stability
Ni2+
-
requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Zn2+
-
requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Zn2+
-
not
Zn2+
-
not
additional information
-
no activation by Fe3+
additional information
-
no activation by Cu2+ or monovalent cations
additional information
divalent cation required for catalysis
additional information
-
the enzyme does not require NaCl for stability or enzymatic activity below 30C
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8-bromo-IDP
-
in excess
Ag2+
-
-
AMP
-
kinetics, substrate inhibition
ATP
-
inhibition of nuclease activity
ATP
-
inhibition of nuclease activity
Ca2+
-
above 5 mM
cAMP
-
IC50 6 mM, weak, competitive
chromoglycate
IC50: 90 mM
cromoglycate
-
5 mM, more than 50% inhibition
dCDP
-
minimal inhibitory concentration 0.010 to 0.050mM
Desdanine
-
irreversible, specific inhibitor, kinetics, ATP protects, not dTDP or bovine serum albumin
dTDP
-
minimal inhibitory concentration 0.001 mM
EDTA
-
kinetics, enhanced by Zn2+
EDTA
-
complete inhibition at 10 mM
ellagic acid
-
inhibits NDPK and suppresses MDA-MB-435S cell growth
epigallocatechin gallate
-
EGCG, inhibits NDPK and suppresses MDA-MB-435S cell growth
GDP
-
at higher concentrations, substrate inhibition
GDP
especially susceptible to substrate inhibition by GDP
GDP
-
competitive inhibition of nuclease activity
GMP
-
erythrocytic kinase, kinetics
Mg2+
-
above 5 mM
NaCl
-
strong inhibition and destabilization at 2 M
p-chloromercuribenzoate
-
pI 7.3-isoform, DTT restores, but not in the presence of urea, ATP or dTDP protects
p-chloromercuribenzoate
-
bovine liver mitochondrial and human erythrocytic enzymes: substrates protect
theophylline
-
-
UDP
-
5 mM, more than 50% inhibition
Urea
-
inactivation occurs at very low urea concentrations (0.5 M)
MgADP-
-
in excess
additional information
-
no inhibition by papaverine
-
additional information
-
no inhibition by Zn2+ alone
-
additional information
-
heat shock causes a non-competitive inhibition of the enzyme, which is partly prevented by ascorbate, effects of heat shock on mitochondrial metabolism overview
-
additional information
-
the acid-induced binding of NDP kinase HA-alpha1-130beta131-152 to ROS photoreceptor membranes is prevented by addition of NaCl or KCl
-
additional information
-
SOS2, a class 3 sucrose-nonfermenting 1-related kinase, interacts with the H2O2 signaling protein NDPK2 and inhibits its autophosphorylation activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Trimethylamine N-oxide
-
concentration of 4 M required for activity, at pH 8 inclusion of low concentrations of NaCl required for activation
additional information
-
nucleoside triphosphate, especially GTP, promotes the dynamic self-assembly of isozyme NDPKB into ordered 20-25 nm diameter filaments in vitro
-
additional information
-
isozyme NDPK3a is highly inducible by glucose and sucrose, other NDPK isozymes are less sensitive to sucrose, overview
-
additional information
-
adenylate kinase from Arabidopsis thaliana stimulates NDPK activity, whereas NDPK inhibits the activity of adenylate kinase, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.033
8-bromo-IDP
-
isozyme A6, 25C
0.06
8-bromo-IDP
-
isozyme B6, 25C
0.024
ADP
-
cosubstrate GTP
0.042
ADP
-
cosubstrate ATP, cytosolic kinase
0.066
ADP
-
isoform alpha
0.073
ADP
-
cosubstrate ATP, membrane-associated kinase
0.079
ADP
-
isozyme NDPK2, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.08
ADP
-
isoform beta
0.108
ADP
-
isozyme NDPK3, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.125
ADP
-
isozyme NDPK3, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.138
ADP
-
isozyme NDPK2, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.392
ADP
-
isozyme NDPK1, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.552
ADP
-
isozyme NDPK1, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.578
ADP
-
isozyme NDPK3, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.6
ADP
-
wild-type, pH 7.5, 30C
0.7
ADP
-
mutant G114R, pH 7.5, 30C
0.707
ADP
-
isozyme NDPK1, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
1.26
ADP
-
isozyme NDPK2, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.126
ATP
-
recombinant His-tagged NDPK
0.19
ATP
-
cosubstrate dTDP, 30C, pH 8.0
0.2 - 0.38
ATP
-
cosubstrate dTDP, two different assays
0.89
ATP
-
cosubstrate GDP, NDP-kinase II, 37C, pH 7.4
1.33
ATP
-
cosubstrate GDP, cytosolic kinase
1.66
ATP
-
cosubstrate GDP, membrane-associated kinase
1.8
ATP
-
isoform alpha
2
ATP
-
NDP-kinase I, 37C, pH 7.4
4.6
ATP
-
isoform beta
0.26 - 0.37
CDP
-
30C, pH 8.0; cosubstrate ATP
0.26 - 0.37
CDP
-
37C, pH 7.6; cosubstrate ATP
0.5 - 0.54
CDP
-
cosubstrate ATP; pH 7.5, 37C
0.5 - 0.54
CDP
-
cosubstrate ATP; cytosolic kinase
0.69
CDP
-
cosubstrate ATP, membrane-associated kinase
5.28
CTP
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
6.72
CTP
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
9.37
CTP
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
10
CTP
-
isoform alpha
12
CTP
-
isoform beta
0.09
dCDP
S69A mutant enzyme, 22C
0.2
dCDP
wild type enzyme, 22C
0.28
dCDP
S119A mutant enzyme, 22C
0.42
dCDP
-
cosubstrate ATP, 30C, pH 8.0
0.5 - 0.54
dCDP
-
cosubstrate ATP, 37C, pH 7.6
0.56
dCDP
-
cosubstrate ATP, pH 7.5, 37C
0.25
dGDP
-
cosubstrate ATP, 37C, pH 7.6
0.07
dTDP
S69A mutant enzyme, 22C
0.08
dTDP
S119A mutant enzyme, 22C
0.1
dTDP
wild type enzyme, 22C
0.17
dTDP
-
cosubstrate ATP, 37C, pH 7.6
0.21 - 0.39
dTDP
-
cosubstrate ATP, two different assays
0.21 - 0.39
dTDP
-
-
0.21 - 0.39
dTDP
-
30C, pH 8.0
0.47
dTDP
pH 7.5, 35C, isozyme NDPK2
0.86
dTDP
pH 7.5, 35C, isozyme NDPK1
0.5 - 0.54
dUDP
-
cosubstrate ATP, 37C, pH 7.6
0.02
GDP
-
pH 7.5, 25C
0.031
GDP
-
cosubstrate ATP, cytosolic kinase
0.036
GDP
-
cosubstrate ATP, membrane-associated kinase
0.041
GDP
-
isoform beta
0.049
GDP
-
isoform alpha
0.091 - 0.1
GDP
-
cosubstrate ATP, NDP-kinase I and II, 37C, pH 7.4
0.2 - 0.38
GDP
-
-
0.07
GTP
-
cosubstrate dTDP, 30C, pH 8.0
0.12
GTP
-
cosubstrate ADP
0.15
GTP
-
isoform alpha
0.45
GTP
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.51
GTP
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.64
GTP
-
isoform beta
0.88
GTP
-
pH 8.0
1.21
GTP
-
pH 8.0, presence of 0.2 M NaCl
1.69
GTP
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
2.13
GTP
-
pH 8.0, presence of 1 M NaCl
2.17
GTP
-
pH 8.0, presence of 0.5 M NaCl
2.24
GTP
-
pH 8.0, presence of 0.2 M NaCl
2.62
GTP
-
pH 8.0, presence of 0.5 M NaCl
2.63
GTP
-
pH 8.0
3
GTP
-
wild-type, pH 7.5, 30C
3.26
GTP
-
pH 8.0, presence of 1 M NaCl
3.8
GTP
-
wild-type, pH 7.5, 30C, presence of 3.8 M NaCl
4.4
GTP
-
mutant G114R, pH 7.5, 30C
0.89
TTP
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
1.34
TTP
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
5.28
TTP
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
0.16
UDP
-
cosubstrate ATP, membrane-associated kinase
0.19
UDP
-
cosubstrate ATP, cytosolic kinase
0.28
UDP
-
cosubstrate ATP, 37C, pH 7.6
4.8
UTP
-
mutant G114R, pH 7.5, 30C
6
UTP
-
mutant G114R, pH 7.5, 30C, presence of 3.8 M NaCl
6.1
UTP
-
wild-type, pH 7.5, 30C
8.1
UTP
-
wild-type, pH 7.5, 30C, presence of 3.8 M NaCl
16
UTP
-
isoform beta
27
UTP
-
isoform alpha
5.5
GTP
-
mutant G114R, pH 7.5, 30C, presence of 3.8 M NaCl
additional information
additional information
-
effect of Mg2+ on kinetic parameters
-
additional information
additional information
-
Km-values of different pI-enzyme variants of ATP or GTP and TDP or CDP as substrates
-
additional information
additional information
-
Michaelis constants of kinases from various sources
-
additional information
additional information
-
effect of pH on kinetic parameters of pI 7.3-enzyme variant from human erythrocytes; Michaelis constants of kinases from various sources
-
additional information
additional information
-
Michaelis constants of kinases from various sources
-
additional information
additional information
-
kinetics of membrane binding of recombinat wild-type isozymes and chimeric mutants NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152 to N-membranes
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.159
8-bromoinosine 5'-diphosphate
Mycobacterium tuberculosis
-
wild type enzyme, at pH 7.5 and 22C
-
0.23
8-bromoinosine 5'-diphosphate
Mycobacterium tuberculosis
-
mutant enzyme D93N, at pH 7.5 and 22C
-
232
ATP
Mycobacterium tuberculosis
-
mutant enzyme D93N, at pH 7.5 and 22C
264
ATP
Mycobacterium tuberculosis
-
wild type enzyme, at pH 7.5 and 22C
2085
ATP
Pisum sativum
-
recombinant His-tagged NDPK
85.2
CTP
Oryza sativa
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
89
CTP
Oryza sativa
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
229
CTP
Oryza sativa
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
9
dCDP
Pisum sativum
Q9SP13
S69A mutant enzyme, 22C
105
dCDP
Pisum sativum
Q9SP13
S119A mutant enzyme, 22C
106
dCDP
Pisum sativum
Q9SP13
wild type enzyme, 22C
13.3
dTDP
Saccharomyces cerevisiae
-
-
17
dTDP
Pisum sativum
Q9SP13
S69A mutant enzyme, 22C
120
dTDP
Pisum sativum
Q9SP13
S119A mutant enzyme, 22C
147
dTDP
Pisum sativum
Q9SP13
wild type enzyme, 22C
38
GDP
Thermus thermophilus
-
pH 7.5, 25 C
17.9
GTP
Oryza sativa
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
27.7
GTP
Oryza sativa
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
49.4
GTP
Oryza sativa
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
14.2
TTP
Oryza sativa
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
33.5
TTP
Oryza sativa
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
55
TTP
Oryza sativa
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
67.6
ADP
Oryza sativa
-
isozyme NDPK2, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
69.9
ADP
Oryza sativa
-
isozyme NDPK1, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
140
ADP
Oryza sativa
-
isozyme NDPK1, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
143
ADP
Oryza sativa
-
isozyme NDPK3, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
154
ADP
Oryza sativa
-
isozyme NDPK3, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
180
ADP
Oryza sativa
-
isozyme NDPK2, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
210
ADP
Oryza sativa
-
isozyme NDPK2, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
310
ADP
Oryza sativa
-
isozyme NDPK3, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
415
ADP
Oryza sativa
-
isozyme NDPK1, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
13
9.1
CTP
Oryza sativa
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
60
16.9
CTP
Oryza sativa
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
60
34.1
CTP
Oryza sativa
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
60
29.2
GTP
Oryza sativa
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
37
35.1
GTP
Oryza sativa
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
37
61.6
GTP
Oryza sativa
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
37
10.4
TTP
Oryza sativa
-
isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
523
16
TTP
Oryza sativa
-
isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
523
25
TTP
Oryza sativa
-
isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25C
523
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.9
GDP
-
competitive inhibition of nuclease activity
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.9
AMP
Mycobacterium tuberculosis
A5U5E1
IC50: 1.9 mM
6
cAMP
Streptomyces coelicolor
-
IC50 6 mM, weak, competitive
26.7
cAMP
Mycobacterium tuberculosis
A5U5E1
IC50: 26.7 mM
90
chromoglycate
Mycobacterium tuberculosis
A5U5E1
IC50: 90 mM
0.002 - 0.003
ellagic acid
Homo sapiens
-
-
0.008 - 0.01
epigallocatechin gallate
Homo sapiens
-
-
1.6
UDP
Mycobacterium tuberculosis
A5U5E1
IC50: 1.6 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0000083
-
autophosphorylation assay
5.4
-
37C, pH 7.6
41.3
wild type isoform NDPK2, using dTDP as substrate, at pH 7.5 and 22C
50
H117A mutant enzyme with dCDP as substrate, 22C
60
S69A/S119A mutant enzyme with dCDP as substrate, 22C
63.98
line R3-1 isoform NDPK3, using dGDP as substrate, at pH 7.5 and 22C
64.43
wild type isoform NDPK3, using dGDP as substrate, at pH 7.5 and 22C
70
H117D/S119A mutant enzyme with dCDP as substrate, 22C
90.55
line R3-1 isoform NDPK2, using dTDP as substrate, at pH 7.5 and 22C
103.5
line R3-1 isoform NDPK3, using dTDP as substrate, at pH 7.5 and 22C
112.6
wild type isoform NDPK3, using dTDP as substrate, at pH 7.5 and 22C
156.6
wild type isoform NDPK2, using dGDP as substrate, at pH 7.5 and 22C
160
S69A mutant enzyme with dCDP as substrate, 22C
207
-
purified extracellular enzyme
283.9
line R3-1 isoform NDPK2, using dGDP as substrate, at pH 7.5 and 22C
340
-
pH 8.0, temperature not specified in the publication
360
-
25C, pH 8.0
550
-
isozyme A6, 25C
700
S69A mutant enzyme with dTDP as substrate, 22C
740
-
isozyme B6, 25C
800
-
25C
800
-
native enzyme
1000
-
about, in absence of NaCl, purified wild-type enzyme
1100
-
about, in absence of NaCl, purified wild-type enzyme
1200
-
about, purified recombinant enzyme; pH 8.0
1250
-
NDP-kinase I, 37C, pH 7.4
1500
-
25C, pH 7.4
1500
-
about, purified recombinant enzyme; pH 8.0
1736
-
30C, pH 8.0
2026
-
25C
2160
-
NDP-kinase II, 37C, pH 7.4
2400
S119A mutant enzyme with dCDP as substrate, 22C
2700
wild type enzyme with dCDP as substrate, 22C
3900
S119A mutant enzyme with dTDP as substrate, 22C
4341
pH 7.5, 30C
5100
wild type enzyme with dTDP as substrate, 22C
6427
-
purified cytosolic enzyme
additional information
-
-
additional information
-
-
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.2
-
isozymes NDPK2 and NDPK3
7.2
-
assembly assay at
7.4
-
assay at
7.5
assay at
7.5
-
assay at
8 - 8.1
-
assay at
8.4
-
isozyme NDPK1
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.5 - 10.5
-
-
6.5 - 10.3
-
about half-maximal activity at pH 6.5 and 10.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
22
-
assay at room temperature
25
-
assay at
25
-
assay at
25
-
assay at
25
-
assay at
25
-
assay at
25
assay at
30
-
assay at
30
-
assay at
30
-
assay at
30
-
assay at
37
-
assay with tubulin-GDP as substrate
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assembly assay at
40 - 50
-
-
40 - 50
-
sharp decrease in activity above 50C
additional information
-
temperature-dependence of NDP kinases
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 60
-
temperature profile, overview
30 - 80
-
temperature profile, overview
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.04
isoelectric focusing
5.07
isoelectric focusing
5.07
isoelectric focusing
5.07
isoelectric focusing
5.07
isoelectric focusing
5.29
isoelectric focusing
5.42
isoelectric focusing
5.43
-
isoelectric focusing
5.43
isoelectric focusing
5.43
isoelectric focusing
5.43
-
isoelectric focusing
5.45
-
isoelectric focusing
5.47
isoelectric focusing
6.6
calculated from the deduced amino acid sequence
8.8
calculated from the deduced amino acid sequence
9.5
calculated from the deduced amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
highest expression
Manually annotated by BRENDA team
-
H50-7209 cell suspension culture
Manually annotated by BRENDA team
enzyme activity and protein level raise during exponential growth, no rise in activity when supply with sucrose is limited
Manually annotated by BRENDA team
-
asexual conidia, presence of enzyme during most developmental stages
Manually annotated by BRENDA team
highly expressed in cotyledons of seedlings; highly expressed in cotyledons of seedlings
Manually annotated by BRENDA team
-
isoforms A,B in the ratio 1:1
Manually annotated by BRENDA team
; increased expression during flower maturation; increased expression during flower maturation
Manually annotated by BRENDA team
the expression level is increased in hemocytes after bacterial challenge
Manually annotated by BRENDA team
the expression level is increased in hemolymph after bacterial challenge
Manually annotated by BRENDA team
-
aerial hyphae, presence of enzyme during most developmental stages
Manually annotated by BRENDA team
highest expression
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
highest expression
Manually annotated by BRENDA team
-
of the mucosa, RBL-2H3 cell line
Manually annotated by BRENDA team
-
mast cell, cell line RBL-2H3
Manually annotated by BRENDA team
-
skeletal muscle
Manually annotated by BRENDA team
-
breast muscle
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
skeletal muscle
-
Manually annotated by BRENDA team
-
submerged mycelium, presence of enzyme during most developmental stages
Manually annotated by BRENDA team
-
presence of enzyme during most developmental stages
Manually annotated by BRENDA team
-
isoforms A,B in the ratio 1:3
Manually annotated by BRENDA team
highest expression
Manually annotated by BRENDA team
highly expressed in roots of seedlings
Manually annotated by BRENDA team
predominant localization in meristematic zones and provascular tissues
Manually annotated by BRENDA team
-
immature seed
Manually annotated by BRENDA team
expression of NDK2 increases dramatically when seedlings germinated in the dark are transferred into light, not expressed when seedlings are grown in the dark; increased expression at day 3-4 in seedlings germinated in the dark, highly expressed in hooks of seedlings; increased expression from day 2-5 in seedlings germinated in the dark, highly expressed in hooks of seedlings
Manually annotated by BRENDA team
-
; i.e. Tobacco Bright Yellow 2 cells
Manually annotated by BRENDA team
additional information
-
tissue distribution
Manually annotated by BRENDA team
additional information
in situ hybridization
Manually annotated by BRENDA team
additional information
-
NDPK1 is the most highly expressed isozyme, followed by isozymes NDPK3a and NDPK3b, the latter is generally expressed at very low extent but increased in inflorescences and dividing cells
Manually annotated by BRENDA team
additional information
HeLa cells are naturally almost devoid of NDPK-D
Manually annotated by BRENDA team
additional information
-
isoform NDPK1 is weakly expressed in leaf sheaths
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
isozyme NDPK2 is localized in the stroma, no NDPK3 in the chloroplasts
Manually annotated by BRENDA team
NDPK2 contains a predicted chloroplast targeting transit peptide of 63 amino acids, import mechanism, overview
Manually annotated by BRENDA team
-
in mycelia, compartmentalization to plasma membrane in darkness and relocalization to cytoplasm under light
Manually annotated by BRENDA team
-
more than 80% of total activity is detected in the cytoplasm
Manually annotated by BRENDA team
-
cytosolic and plasma membrane-associated enzymes are quite similar, if not identical
-
Manually annotated by BRENDA team
-
cytosolic and plasma membrane-associated enzymes are quite similar, if not identical
Manually annotated by BRENDA team
-
isozyme NDPK1
Manually annotated by BRENDA team
Dictyostelium discoideum AX2, Leishmania amazonensis LV78, Pseudomonas aeruginosa 8822
-
-
-
Manually annotated by BRENDA team
-
enzyme is secreted to the culture medium
-
Manually annotated by BRENDA team
-
enzyme is secreted into the culture medium
-
Manually annotated by BRENDA team
-
the enzyme is secreted
-
Manually annotated by BRENDA team
Leishmania amazonensis LV78
-
enzyme secretion
-
-
Manually annotated by BRENDA team
-
enzyme is secreted to the culture medium
-
-
Manually annotated by BRENDA team
-
microsomal membrane
-
Manually annotated by BRENDA team
-
NDPK is attached to the outer side of the inner the membrane
Manually annotated by BRENDA team
structural basis of NDPK-D/mitochondrial membrane interactions via binding of cardiolipin, overview
Manually annotated by BRENDA team
-
structural basis of NDPK-D/mitochondrial membrane interactions, overview
Manually annotated by BRENDA team
-
in rat liver mitochondria, all NDPK activity of the outer compartment is associated with the cytoplasmic surface of the outer mitochondrial membrane
Manually annotated by BRENDA team
-
dual cellular localization in mitochondria and cytosol
Manually annotated by BRENDA team
-
after 2 h from heat shock, rate of ADT/ATP exchange by adenine nucleotide translocator activity, adenylate kinase activity EC 2.7.4.3 and nucleoside diphosphate kinase are inhibited in a non-competitive-like manner. Externally added ascorbate prevents the inhibition
Manually annotated by BRENDA team
-
intermembrane space; intermembrane space of mitochondria
Manually annotated by BRENDA team
-
isozyme NDPK3a is predominantly localized in the mictochondria
Manually annotated by BRENDA team
located in the mitochondrial intermembrane/cristae space
Manually annotated by BRENDA team
-
associated as complex of NDPK B and G proteinbetagamma-dimers
Manually annotated by BRENDA team
-
in mycelia, compartmentalization to plasma membrane in darkness and relocalization to cytoplasm under light
Manually annotated by BRENDA team
-
isozyme NDPK2
Manually annotated by BRENDA team
additional information
-
overview: localization, physiological role
-
Manually annotated by BRENDA team
additional information
-
subcellular localization analysis, overview
-
Manually annotated by BRENDA team
additional information
subcellular localization analysis, overview
-
Manually annotated by BRENDA team
additional information
-
the NDPK isozymes are localized in different subcelllar compartments, distribution, overview
-
Manually annotated by BRENDA team
additional information
no NDPK3 in the chloroplasts; no NDPK3 in the chloroplasts
-
Manually annotated by BRENDA team
additional information
-
the enzyme colocalises with actin and calmodulin, but not with macropinosomes
-
Manually annotated by BRENDA team
additional information
-
the enzyme colocalises with actin and calmodulin, but not with macropinosomes
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Acinetobacter baumannii (strain SDF)
Acinetobacter baumannii (strain SDF)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Encephalitozoon cuniculi (strain GB-M1)
Escherichia coli (strain K12)
Giardia intestinalis (strain ATCC 50803 / WB clone C6)
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Oryza sativa subsp. japonica
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma cruzi (strain CL Brener)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
17000 - 18000
-
SDS-PAGE
701452
18000
-
SDS-PAGE
702511
30130
-
light scattering
673747
32100
-
gel filtration and multiangle laser light scattering
723728
33000
gel filtration
722131
36600
-
gel filtration of wild-type, 35C
673696
42000
in the presence of 0.5 M NaCl, sedimentation velocity
702706
45000
-
dimer, gel filtration
642644
55000
-
gel filtration
642657
60000
-
gel filtration
660576
62120
-
light scattering
673747
63000
-
NDPK IIpn isoform, gel filtration
701452
65000 - 68000
-
-
694064
66000
-
sedimentation equilibrium
642658
66000
-
gel filtration
642663
66000
gel filtration, mitochondrial isoform
642671
68000 - 70000
-
FPLC gel filtration
642651
70000
-
-
642633
70000
-
gel filtration
642644
70000
-
-
642661
70000 - 75000
-
gel filtration
642641
71000
-
NDPK In isoform, gel filtration
701452
80000
-
erythrocytic enzyme variants pI 5.4 and pI 6.8, gel filtration
642661
80000 - 100000
-
erythrocytic enzyme variants
642656
84000
-
erythrocytic enzyme variants pI 6.3 and pI 7.3, gel filtration
642661
89000
dynamic lights cattering measurements
726567
92000
-
isozyme I, gel filtration
642646
93000
-
erythrocytic enzyme variant pI 5.8, gel filtration
642661
99700
-
sucrose density gradient centrifugation
642640
100000
-
gel filtration
642636
100000
-
gel filtration
642649
100000
-
-
642661
100000
-
erythrocytic enzyme variant pI 8.3, gel filtration
642661
100000
-
-
642661
102000
-
high speed sedimentation equilibrium, meniscus depletion procedure
642653
102000
-
-
642661
103000
-
sucrose density gradient centrifugation
642637
103000
-
heart mitochondrial enzyme
642661
104000
-
in the presence of 2 M NaCl, sedimentation velocity
702706
105000
-
gel filtration
642634
105000
-
gel filtration
701497
106800
-
gel filtration of wild-type, 35C, of mutant G114R at 25 and 35C
673696
107000
gel filtration
662280
108000
-
gel filtration
642637
109000
-
liver mitochondrial enzyme, gel filtration, sedimentation equilibrium
642661
110000
-
-
209854
110000
-
isozyme II, gel filtration
642646
111000
in the presence of 2 M NaCl, sedimentation velocity
702706
112000
-
gel filtration
674397
112000
-
estimated from amino acid sequence
701497
114000
-
in the presence of 0.5 M NaCl, sedimentation velocity
702706
125000
-
gel filtration
642667
125000
gel filtration
662729
130000
-
gel filtration
642666
additional information
-
N-terminal sequence homology of kinases from different organisms
642645
additional information
-
amino acid composition
642646
additional information
-
amino acid sequence of isozyme I deduced from cDNA sequence
642647
additional information
-
N-terminal sequence homology of kinases from different organisms
642648
additional information
-
N-terminal sequence homology of kinases from different organisms
642649
additional information
-
comparison of kinase amino acid sequences
642661
additional information
-
-
642661
additional information
-
comparison of kinase amino acid sequences
642661
additional information
-
-
642661
additional information
-
the enzyme has a molecular weight of 60-120 kDa
692061
additional information
-
N-terminal sequence homology of kinases from different organisms
817
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 17000, SDS-PAGE
?
-
x * 1700, SDS-PAGE
?
-
x * 36000, SDS-PAGE under non-reducing conditions
?
-
x * 21500, SDS-PAGE
?
-
x * 18000, SDS-PAGE
?
-
x * 16000, deduced from gene sequence
?
-
x * 16500, SDS-PAGE
?
x * 18000, SDS-PAGE
?
-
x * 18000, SDS-PAGE
?
-
x * 17200, isoform beta, x * 17300, isoform alpha, deduced from gene sequence, major form is alpha
?
-
x * 15000, SDS-PAGE
?
-
x * 14400, SDS-PAGE
?
x * 17400, calculated from the deduced amino acid sequence; x * 25500, calculated from the deduced amino acid sequence; x * 25700, calculated from the deduced amino acid sequence
?
x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE; x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms
?
x * 19300, SDS-PAGE of recombinant protein including His-tag
?
x * 18600, SDS-PAGE, x * 18675, mass spectrometry, recombinant protein including 19 amino acid extension
?
-
x * 16810, about, MALDI-TOF mass spectrometry
?
x * 17800, about, MALDI-TOF mass spectrometry
?
x * 17810, about, MALDI-TOF mass spectrometry
?
x * 17820, about, MALDI-TOF mass spectrometry
?
x * 18590, about, MALDI-TOF mass spectrometry
?
x * 17800, about, MALDI-TOF mass spectrometry
?
x * 17810, about, MALDI-TOF mass spectrometry
?
x * 18600, about, MALDI-TOF mass spectrometry
?
x * 18590, about, MALDI-TOF mass spectrometry
?
x * 18600, about, MALDI-TOF mass spectrometry