Cloned (Comment) | Organism |
---|---|
fusion of extra sequence containing hexa-His-tag at amino-terminus of the enzyme facilitates folding and activation in Escherichia coli | Halobacterium salinarum |
General Stability | Organism |
---|---|
amino-terminal extension enhances dimer to hexamer assembly and stabilizes the structure in low salt | Halobacterium salinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | P61136 | - |
- |
Halobacterium salinarum JCM 11081 | P61136 | - |
- |
Renatured (Comment) | Organism |
---|---|
refolding of the His-tagged enzyme after denaturation is much more efficient than refolding of the untagged enzyme | Halobacterium salinarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
340 | - |
pH 8.0, temperature not specified in the publication | Halobacterium salinarum |
Subunits | Comment | Organism |
---|---|---|
dimer | folded His-tagged enzyme isolated from Escherichia coli forms a hexamer in both 0.2 M and 3.8 M NaCl at 30 °C, while the native enzyme purified from Halobacterium salinarum dissociates to dimer in 0.2 M NaCl | Halobacterium salinarum |
hexamer | folded His-tagged enzyme isolated from Escherichia coli forms a hexamer in both 0.2 M and 3.8 M NaCl at 30 °C, while the native enzyme purified from Halobacterium salinarum dissociates to dimer in 0.2 M NaCl | Halobacterium salinarum |
Synonyms | Comment | Organism |
---|---|---|
HsNDK | - |
Halobacterium salinarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Halobacterium salinarum |