Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysE | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
GDP | pH 7.5, 25°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
4 * 15000, calculated from the deduced amino acid sequence, SDS-PAGE, native mass by gel filtration | Thermus thermophilus |
60000 | - |
gel filtration | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | Thermus thermophilus | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | ADP + NTP | - |
? | |
ATP + NDP | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | ADP + NTP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Thermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate | two-step ping-pong mechanism | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CDP | - |
Thermus thermophilus | ADP + CTP | - |
? | |
ATP + CDP | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + CTP | - |
? | |
ATP + GDP | - |
Thermus thermophilus | ADP + GTP | - |
? | |
ATP + GDP | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + GTP | - |
? | |
ATP + NDP | - |
Thermus thermophilus | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | Thermus thermophilus | ADP + NTP | - |
? | |
ATP + NDP | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleosides triphosphates | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + NTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 15000, calculated from the deduced amino acid sequence, SDS-PAGE, native mass by gel filtration | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38 | - |
GDP | pH 7.5, 25 °C | Thermus thermophilus |