General Stability | Organism |
---|---|
unstable in dilute solutions, bovine serum albumin stabilizes, not DTT | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | kinetics, substrate inhibition | Bos taurus | |
MgADP- | in excess | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of Mg2+ on kinetic parameters | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
mitochondrion | dual cellular localization in mitochondria and cytosol | Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bos taurus | |
Mg2+ | true substrates: Mg-ADP and Mg-ATP | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103000 | - |
sucrose density gradient centrifugation | Bos taurus |
108000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate | mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
360 | - |
25°C, pH 8.0 | Bos taurus |
Storage Stability | Organism |
---|---|
0-2°C, 2 mg bovine serum albumin/ml, 0.04% NaN3, at least 1 month | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-methylene diphosphonate | - |
Bos taurus | ADP + ? | - |
? | |
ATP + ADP | best substrate | Bos taurus | ADP + ATP | - |
? | |
ATP + CDP | reverse reaction at 21% the rate of ATP plus ADP | Bos taurus | ADP + CTP | - |
r | |
ATP + UDP | reverse reaction at 32% the rate of the reaction ATP plus ADP | Bos taurus | ADP + UTP | - |
r | |
CTP + CDP | reaction at 15% the rate of ATP | Bos taurus | CDP + CTP | - |
? | |
GTP + ADP | reaction at 12% the rate of ATP | Bos taurus | GDP + ATP | - |
? | |
NTP + nucleoside diphosphate | the nature of the nucleoside triphosphate controls the rate of the reaction | Bos taurus | NDP + NTP | - |
? | |
UTP + UDP | reaction at 31% the rate of ATP | Bos taurus | UDP + UTP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
1 h, 0.0018 mg enzyme/ml, in 0.1 M phosphate buffer, pH 7, more than 50% loss of activity, 2 mg/ml bovine serum albumin plus 2 mM Mg-ADP or 5 mM free ADP protect, not MgCl2 or free ADP alone | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
inactivation below | Bos taurus |