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Literature summary for 2.7.4.6 extracted from

  • Tokunaga, H.; Izutsu, K.; Arai, S.; Yonezawa, Y.; Kuroki, R.; Arakawa, T.; Tokunaga, M.
    Dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacterium Chromohalobacter salexigens DSM3043: Both residues 134 and 136 are critical for the tetramer assembly (2010), Enzyme Microb. Technol., 46, 129-135.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21star(DE3) cells Chromohalobacter salexigens

Protein Variants

Protein Variants Comment Organism
C139S the mutant forms a dimer Chromohalobacter salexigens
E136T the mutant forms a dimer Chromohalobacter salexigens
G134A/E136T the mutant forms a tetramer Chromohalobacter salexigens
G134A/E136T/C139S the mutant forms a tetramer Chromohalobacter salexigens
G134A/N135A/E136T the mutant forms a tetramer Chromohalobacter salexigens
G134E the mutant forms a dimer Chromohalobacter salexigens
G134E/E136T the mutant forms a dimer Chromohalobacter salexigens
G134EA the mutant forms a dimer Chromohalobacter salexigens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15159
-
2 * 15159, calculated from amino acid sequence Chromohalobacter salexigens
33000
-
gel filtration Chromohalobacter salexigens

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens Q1QTL2
-
-
Chromohalobacter salexigens DSM 3043 Q1QTL2
-
-

Purification (Commentary)

Purification (Comment) Organism
ATP-agarose-affinity column chromatography, and gel filtration Chromohalobacter salexigens

Subunits

Subunits Comment Organism
homodimer 2 * 15159, calculated from amino acid sequence Chromohalobacter salexigens

Synonyms

Synonyms Comment Organism
NDK
-
Chromohalobacter salexigens
nucleoside diphosphate kinase
-
Chromohalobacter salexigens