EC Number |
---|
2.7.4.6 | - |
2.7.4.6 | crystal structure and crystal-ADP interaction |
2.7.4.6 | crystallized at 24°C using polyethylene glycol 4000 as precipitant. The crystal is hexagonal, belonging to the space group P63, with unit-cell parameters a = b = 72.89, c = 100.87 A. The asymmetric unit contains two subunits of NDP kinase |
2.7.4.6 | hanging drop vapor diffusion method |
2.7.4.6 | hanging drop vapor diffusion method at room temperature |
2.7.4.6 | hanging drop vapour diffusion |
2.7.4.6 | hanging drop vapour-diffusion method, 0.002 ml protein solution consisting of 10 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM EDTA equilibrated against 0.5 ml reservoir solution consisting of 2.25 M ammonium formate and 0.1 M HEPES buffer, pH 7.25, X-ray diffraction structure determination at 2.0 A resolution |
2.7.4.6 | hanging-drop vapor diffusion method |
2.7.4.6 | hanging-drop vapor diffusion method at 18°C. Crystallized in a free state and a substrate-bound form with CDP. The structures are solved to a resolution of 2.35 and 2.2 A, respectively. Crystals with the apo-form are obtained with His6-tagged enzyme, whereas the untagged form is used for co-crystallization with the nucleotide |
2.7.4.6 | hanging-drop vapor-diffusion method |