Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Halobacterium salinarum |
Protein Variants | Comment | Organism |
---|---|---|
D112S | the mutant shows reduced specific activity compared to the wild type enzyme | Halobacterium salinarum |
D58G | the mutant shows strongly reduced specific activity compared to the wild type enzyme | Halobacterium salinarum |
D58G/D63G | the mutant shows no refolding enhancement and strongly reduced specific activity compared to the wild type enzyme | Halobacterium salinarum |
D63G | the mutant shows slightly reduced specific activity compared to the wild type enzyme | Halobacterium salinarum |
G114D | the mutant, which forms a dimer in low salt solution, is strongly stabilized by His-tag to form a stable hexamer and shows strongly reduced specific activity compared to the wild type enzyme | Halobacterium salinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ATP-agarose column chromatography, and gel filtration | Halobacterium salinarum |
Renatured (Comment) | Organism |
---|---|
in 50 mM Tris-HCl buffer containing 3 M NaCl, at 4°C. Refolding is extremely slow, reaching the nearly full activity at 72 h | Halobacterium salinarum |
Synonyms | Comment | Organism |
---|---|---|
NDK | - |
Halobacterium salinarum |
nucleoside diphosphate kinase | - |
Halobacterium salinarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
heating at 90°C for 5 min is sufficient to fully inactivate the protein | Halobacterium salinarum |