Cloned (Comment) | Organism |
---|---|
transient expression of GFP-tagged wild-type and mutant NDPK2s in Pisum sativum protoplasts | Pisum sativum |
Protein Variants | Comment | Organism |
---|---|---|
A77T | site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview | Pisum sativum |
R63D | site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview | Pisum sativum |
R74D | site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | chloroplast stroma | Pisum sativum | 9507 | - |
chloroplast stroma | NDPK2 contains a predicted chloroplast targeting transit peptide of 63 amino acids, import mechanism, overview | Pisum sativum | 9570 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17400 | - |
x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
18500 | - |
x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
18500 | - |
x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE | Pisum sativum |
25000 | - |
x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
25000 | - |
x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pisum sativum | key enzyme involved in the homeostasis of nucleoside triphosphates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
two mature isoforms of NDPK2 in plastids | - |
Pisum sativum | P47923 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | 25000 Da, precursor protein, 18500 Da and 17400 Da, mature enzyme forms starting at amino acids 65 and 78 resp. | Pisum sativum |
Purification (Comment) | Organism |
---|---|
native enzyme from chloroplasts to homogeneity, from cytosol and membranes | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | key enzyme involved in the homeostasis of nucleoside triphosphates | Pisum sativum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms | Pisum sativum |
? | x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
NDPK2 | - |
Pisum sativum |
nucleoside diphosphate kinase 2 | - |
Pisum sativum |