Information on EC 2.6.1.7 - kynurenine-oxoglutarate transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.6.1.7
-
RECOMMENDED NAME
GeneOntology No.
kynurenine-oxoglutarate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-tryptophan degradation XI (mammalian, via kynurenine)
-
-
Metabolic pathways
-
-
tryptophan metabolism
-
-
Tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-kynurenine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on 3-hydroxykynurenine. The product 4-(2-aminophenyl)-2,4-dioxobutanoate is converted into kynurenate by a spontaneous reaction.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-38-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
Debaryomyces globosus
low activity
-
-
Manually annotated by BRENDA team
yeast
-
-
Manually annotated by BRENDA team
Hansenula schneggii
yeast
-
-
Manually annotated by BRENDA team
isoforms KAT I and KAT II, no change of enzyme activity 24 and 72 h after transient global ischemia episode
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
black tiger prawn
-
-
Manually annotated by BRENDA team
Donryu
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Saccharomyces fragilis
low activity
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
KAT-II is the enzymatic isoform that mainly accounts for the synthesis of cerebral kynurenic acid
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobutyrate + glyoxylate
?
show the reaction diagram
-
-
-
-
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3-(2,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
slightly better than L-kynurenine
-
?
3-hydroxykynurenine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
3-hydroxykynurenine + pyruvate
?
show the reaction diagram
-
-
-
?
L-2-aminoheptane-1,7-dioate + 2-oxoglutarate
2-oxoheptane-1,7-dioate + L-glutamate
show the reaction diagram
-
at 39.7% of the activity with 2-aminohexane-1,6-dioate
-
?
L-2-aminohexane-1,6-dioate + 2-oxoglutarate
2-oxohexane-1,6-dioate + L-glutamate
show the reaction diagram
Hansenula schneggii
-
at about 60% the rate of L-kynurenine
-
?
L-3-hydroxykynurenine + 2-oxoglutarate
xanthurenic acid + L-glutamate
show the reaction diagram
L-4-chloro-kynurenine + 2-oxoglutarate
L-7-chloro-kynurenic acid + L-glutamate
show the reaction diagram
-
-
-
?
L-5-chloro-kynurenine + 2-oxoglutarate
L-6-chloro-kynurenic acid + L-glutamate
show the reaction diagram
-
-
-
?
L-alanine + 2-oxobutanoate
pyruvate + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-alanine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-asparagine + 2-oxobutanoate
2-oxosuccinamic acid + 2-aminobutanoate
show the reaction diagram
L-asparagine + glyoxylate
?
show the reaction diagram
-
KAT II has high transamination activity toward L-asparagine
-
-
?
L-cysteine + 2-oxobutanoate
2-aminobutanoate + 3-mercapto-2-oxopropanoate
show the reaction diagram
L-cysteine + 2-oxobutanoate
? + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-cysteine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-glutamine + 2-oxobutanoate
2-oxoglutarate + 2-aminobutanoate
show the reaction diagram
L-glutamine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-histidine + 2-oxobutanoate
3-(1H-imidazol-4-yl)-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
L-histidine + 2-oxoglutarate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-histidine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxo-4-methylthiobutyrate
?
show the reaction diagram
L-kynurenine + 2-oxo-4-methylthiobutyrate
kynurenate + L-methionine
show the reaction diagram
L-kynurenine + 2-oxo-4-methylthiobutyrate
kynurenic acid + L-methionine
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxoadipate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxoadipate
kynurenic acid + 2-aminoadipate + H2O
show the reaction diagram
L-kynurenine + 2-oxobutanoate
4-(2-aminophenyl)-2,4-dioxobutanoate + 2-aminobutanoate
show the reaction diagram
L-kynurenine + 2-oxobutyrate
?
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxobutyrate
kynurenate + ?
show the reaction diagram
L-kynurenine + 2-oxobutyrate
kynurenic acid + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxobutyrate
kynurenic acid + 2-aminobutanoate + H2O
show the reaction diagram
L-kynurenine + 2-oxocaproate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxocaproic acid
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxocaproic acid
kynurenic acid + 2-aminohexanoate
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxoglutarate
4-(2-aminophenyl)2,4-dioxobutanoate + L-glutamate
show the reaction diagram
L-kynurenine + 2-oxoglutarate
?
show the reaction diagram
-
poor cosubstrate for KAT III
-
-
?
L-kynurenine + 2-oxoglutarate
kynurenate + L-glutamate
show the reaction diagram
L-kynurenine + 2-oxoglutarate
kynurenic acid + L-glutamate
show the reaction diagram
L-kynurenine + 2-oxoglutarate
kynurenic acid + L-glutamate + H2O
show the reaction diagram
L-kynurenine + 2-oxohexanedioate
kynurenic acid + 2-aminohexanedioate + H2O
show the reaction diagram
L-kynurenine + 2-oxohexanoate
kynurenic acid + L-norleucine + H2O
show the reaction diagram
Hansenula schneggii
-
-
-
?
L-kynurenine + 2-oxoisocaproate
4-(2-aminophenyl)-2,4-dioxobutanoate + L-leucine
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxoisohexanoate
kynurenic acid + 2-aminoisohexanoate + H2O
show the reaction diagram
L-kynurenine + 2-oxoisopentanoate
kynurenic acid + 2-aminoisopentanoate + H2O
show the reaction diagram
-
poor substrate
-
?
L-kynurenine + 2-oxomethylthiobutyric acid
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxopentanoate
kynurenic acid + L-norvaline + H2O
show the reaction diagram
L-kynurenine + 2-oxovalerate
?
show the reaction diagram
L-kynurenine + 2-oxovalerate
kynurenic acid + 2-aminopentanoate
show the reaction diagram
-
-
-
?
L-kynurenine + 3-indole-2-oxopropanoate
kynurenic acid + L-tryptophan + H2O
show the reaction diagram
L-kynurenine + 3-methyl-2-oxobutanoate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 3-methyl-2-oxopentanoate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 3-methyl-2-oxopentanoate
kynurenic acid + L-isoleucine + H2O
show the reaction diagram
-
poor substrate
-
?
L-kynurenine + 3-phenyl-2-oxopropanoate
kynurenic acid + L-phenylalanine + H2O
show the reaction diagram
L-kynurenine + 4-methyl-2-oxopentanoate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 4-methyl-2-oxovalerate
kynurenic acid + 2,4-dimethylvalerate + H2O
show the reaction diagram
5.9% of activity with 2-oxoglutarate
-
?
L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
kynurenic acid + L-methionine + H2O
show the reaction diagram
L-kynurenine + glyoxylate
4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
show the reaction diagram
L-kynurenine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + glyoxylate
kynurenic acid + glycine
show the reaction diagram
L-kynurenine + glyoxylate
kynurenic acid + glycine + H2O
show the reaction diagram
L-kynurenine + hydroxyphenylpyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + hydroxyphenylpyruvate
kynurenic acid + L-tyrosine
show the reaction diagram
-
-
-
?
L-kynurenine + indo-3-pyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + indol-3-ylpyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + indole-3-pyruvate
kynurenic acid + L-tryptophan
show the reaction diagram
-
-
-
?
L-kynurenine + mercaptopyruvate
4-(2-aminophenyl)-2,4-dioxobutanoate + L-cysteine
show the reaction diagram
-
-
-
-
?
L-kynurenine + mercaptopyruvate
?
show the reaction diagram
L-kynurenine + mercaptopyruvate
kynurenic acid + ?
show the reaction diagram
-
-
-
?
L-kynurenine + oxaloacetate
4-(2-aminophenyl)-2,4-dioxobutanoate + 2-aminosuccinate
show the reaction diagram
-
-
-
-
?
L-kynurenine + oxaloacetate
?
show the reaction diagram
L-kynurenine + oxaloacetate
kynurenate + L-aspartate
show the reaction diagram
L-kynurenine + oxaloacetate
kynurenic acid + L-aspartate
show the reaction diagram
-
-
-
?
L-kynurenine + oxaloacetate
kynurenic acid + L-aspartate + H2O
show the reaction diagram
L-kynurenine + p-hydroxyphenylpyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + phenylpyruvate
4-(2-aminophenyl)-2,4-dioxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + phenylpyruvate
?
show the reaction diagram
L-kynurenine + phenylpyruvate
kynurenic acid + L-phenylalanine
show the reaction diagram
-
-
-
?
L-kynurenine + phenylpyruvate
phenylalanine + L-glutamate + H2O
show the reaction diagram
43% of activity with 2-oxoglutarate
-
?
L-kynurenine + pyruvate
4-(2-aminophenyl)-2,4-dioxobutanoic acid + L-alanine
show the reaction diagram
-
-
-
-
ir
L-kynurenine + pyruvate
4-(2-aminophenyl)2,4-dioxobutanoate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + pyruvate
?
show the reaction diagram
L-kynurenine + pyruvate
kynurenic acid + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + pyruvate
kynurenic acid + L-alanine + H2O
show the reaction diagram
L-leucine + 2-oxobutanoate
2-oxoisocaproate + 2-aminobutanoate
show the reaction diagram
-
-
-
-
?
L-leucine + 2-oxobutanoate
alpha-ketoleucine + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
L-lysine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-methionine + 2-oxobutanoate
4-methylsulfanyl-2-oxobutanoate + 2-aminobutanoate
show the reaction diagram
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + glutamate
show the reaction diagram
L-methionine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-norleucine + 2-oxoglutarate
2-oxohexanoate + L-glutamate
show the reaction diagram
-
at about 30% the rate of L-kynurenine
-
?
L-phenylalanine + 2-oxobutanoate
phenylpyruvate + 2-aminobutanoate
show the reaction diagram
L-phenylalanine + 2-oxoglutarate
phenylpyruvate + L-glutamate
show the reaction diagram
-
-
-
?
L-phenylalanine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-serine + 2-oxobutanoate
3-hydroxy-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-serine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-tryptophan + 2-oxobutanoate
3-indole-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
L-tryptophan + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-tyrosine + 2-oxobutanoate
3-(4-hydroxyphenyl)-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
L-tyrosine + 2-oxoglutarate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
L-tyrosine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-vinylglycine
2-oxobutyrate + NH3
show the reaction diagram
Hansenula schneggii
-
deamination at 1% the rate of kynurenine, suicide substrate
-
?
L-vinylglycine + 1-propanethiol
S-n-propylhomocysteine
show the reaction diagram
Hansenula schneggii
-
gamma-addition reaction in the presence of alkanethiols
-
?
L-vinylglycine + 2-oxobutyrate
2-aminobutyrate + 2-oxo-3-butenoate
show the reaction diagram
Hansenula schneggii
-
-
-
?
L-vinylglycine + ethanethiol
ethionine
show the reaction diagram
Hansenula schneggii
-
gamma-addition reaction in the presence of alkanethiols
i.e. S-ethylhomocysteine
?
L-vinylglycine + methanethiol
methionine
show the reaction diagram
Hansenula schneggii
-
gamma-addition reaction in the presence of alkanethiols
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-kynurenine + 2-oxoglutarate
kynurenic acid + L-glutamate + H2O
show the reaction diagram
additional information
?
-
B1B6U5
the purified recombinant KAT shows dose-dependent inhibition effect on the growth of equine babesial parasite, Babesia caballi, in in vitro culture
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
additional information
-
pyridoxamine 5'-phosphate is not a cofactor
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
approx. 4fold increase in activity at pH 6.4
additional information
-
not activated by chloride salts of Mg2+, Mn2+, Na+, K+ or NH4+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-2-amino-4-(4-(ethylsulfonyl)phenyl)-4-oxobutanoic acid
-
71% inhibition at 1 mM, 63% inhibition at 0.1 mM, poor substrate that undergo slow transamination, KM-value 4.5 mM, Kcat-value 0.15 1/sec, pH 7.5, 25C
(S)-(-)-9-(4-aminopiperazin-1-yl)-8-fluoro-3-methyl-6-oxo-2,3-dihydro-6H-1-oxa-3a-azaphenalene-5-carboxylic acid
-
BFF-122
(S)-(4)-(ethylsulfonyl)benzoylalanine
-
no inhibition of the human enzyme
(S)-4-ethylsulfonylbenzoylalanine
-
specific inhibitor of KAT II
(S)-9-(4-aminopiperazine-1-yl)-8-fluoro-3-methyl-6-oxo-2,3,5,6-tetrahydro-4H-1-oxa-3a-azaphenalene-5-carboxylic acid
-
BFF 122, KAT II activity is totally blocked at 0.01 mM, 43% inhibition of KAT II at 0.001 mM, 26% inhibition of KAT I at 1 mM
2-Aminoadipate
-
-
2-oxoadipate
-
-
2-oxoglutarate
3-(2-carboxyethyl)-5-chloro-1H-indole-2-carboxylic acid
-
-
3-(2-carboxyethyl)-5-nitro-1H-indole-2-carboxylic acid
-
-
3-hydroxykynurenine
3-indolepropionic acid
3-Methyl-2-benzothiazolone hydrazone hydrochloride
Hansenula schneggii
-
0.1 mM, complete inhibition, reversible by pyridoxal phosphate
4-carboxy-3-hydroxyphenylglycine
-
inhibition of brain KAT II
4-chloromercuribenzoate
5-(2-(3-chlorophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
-
-
5-(2-(4-bromophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
-
-
5-(2-(4-chlorophenyl) hydrazono)-6-ethoxy-6-oxohexanoic acid
-
-
5-bromo-3-(2-carboxyethyl)-1H-indole-2-carboxylic acid
-
-
6-ethoxy-5-(2-(4-iodophenyl)hydrazono)-6-oxohexanoic acid
-
-
6-ethoxy-5-(2-(4-methoxyphenyl)hydrazono)-6-oxohexanoic acid
-
-
6-ethoxy-5-(2-(4-nitrophenyl)hydrazono)-6-oxohexanoic acid
-
-
6-ethoxy-6-oxo-5-(2-p-tolylhydrazono)hexanoic acid
-
-
adipate
alpha-aminoadipate
-
strong inhibition of KAT II
amino-2,3-dihydro-5-methyl-3-oxo-4-isoxazolepropionic acid
-
inhibitory in whole cells
aminoadipate
aminooxyacetic acid
-
inhibitory in whole cells
anthranilate
-
weak inhibition
azelaic acid
-
-
cysteine
cysteine sulfinate
-
poor substrate that undergoes slow transamination, KM-value 12.3 mM, pH 7.5, 25C
Decanoic acid
-
-
Dicarboxylic acids
Diethylglutaric acid
-
-
Dimethylglutaric acid
-
-
-
DL-indole-3-lactic acid
EGTA
-
strong inhibition at pH 6.8 and Ca2+ concentrations below 0.5 mM
glutamate
glutamine
hydroxylamine
indole-3-acetic acid
-
-
Indole-3-propionic acid
Indole-3-pyruvate
-
5 mM, complete inhibition
indole-3-pyruvic acid
-
-
iodoacetate
Isonicotinic acid hydrazide
-
anti-tuberculosis drug, enzyme is inhibited in animals fed with a diet containing isonicotinic acid hydrazide and by injection of the drug
KCN
-
complete inhibition
Kynurenate
-
weak inhibition
L- glutamate
-
5 mM decreases KAT II activity
L-2-amino-4-phosphonobutyric acid
-
inhibition of brain KAT II
L-2-aminoadipate
-
-
L-4-chlorokynurenine
-
-
L-5-chlorokynurenine
-
-
L-asparagine
L-aspartate
L-Cycloserine
Hansenula schneggii
-
0.1 mM, 88% inhibition, reversible by pyridoxal phosphate
L-cysteate
-
5.38 mM, 50% inhibition of brain KAT I, 1.55 mM, 50% inhibition of brain KAT II
L-cysteine
L-cysteine sulfinate
-
0.002 mM, 50% inhibition of brain KAT II
L-glutamate
L-glutamine
L-histidine
L-Homocysteate
-
5.0 mM, 50% inhibition of brain KAT I, 2.1 mM, 50% inhibition of brain KAT II
L-Homocysteine sulfinate
-
1.59 mM, 50% inhibition of brain KAT II
L-kynurenine
L-leucine
L-lysine
L-methionine
L-Penicillamine
Hansenula schneggii
-
0.1 mM, 62% inhibition, reversible by pyridoxal phosphate
L-phenylalanine
L-serine-O-phosphate
-
inhibition of brain KAT II
L-tryptophan
methionine
N-ethylmaleimide
Hansenula schneggii
-
-
N-omega-nitro-L-arginine
-
-
nicotinate
-
weak inhibition
phenylalanine
;
phenylhydrazine
Hansenula schneggii
-
0.1 mM, 81% inhibition, reversible by pyridoxal phosphate
phosphate
-
-
pimelate
pyridoxal 5'-phosphate
-
at high concentrations
quinolinic acid
-
strong inhibition at pH 6.8 and Ca2+ concentrations below 0.5 mM
quisqualate
Quisqualic acid
-
1 mM, isoform KAT I, 81% residual activity, isoform KAT II, 1% residual activity
S-dichlorovinyl-L-cysteine
Sulfate or phosphate esters of steroids
-
-
-
trans-3-indolacrylic acid
-
-
trans-pyrrolidine-2,4-dicarboxylate
-
inhibition of brain KAT II
-
Tris amine
-
the commonly used buffer for KAT I assays greatly inhibits KAT I around neutral pH conditions, but shows no inhibition at basic pH condition
tryptophan
xanthurenate
-
weak inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
L-cysteine
at 1.25 mM, 3fold stimulation, at 10 mM, 70% inhibition
L-kynurenine
-
allosterically regulated
L-tryptophan
-
2 mM, 84% increase in activity of heart KAT at pH 8.0
pyridoxamine 5'-phosphate
Hansenula schneggii
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
21.3 - 92.7
2-aminobutanoate
0.00241 - 5.7
2-oxo-4-methylthiobutyrate
0.01 - 20.9
2-oxoadipate
3
2-oxobutanoate
-
pH 7.5, 45C
0.000217 - 42.2
2-oxobutyrate
1.5
2-oxocaproate
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
0.5 - 10.4
2-oxocaproic acid
0.013 - 8.1
2-oxoglutarate
1
2-oxoisocaproate
5.3
2-oxoisocaproic acid
-
in 100 mM boric acid buffer, pH 9.0, at 45C
1.2 - 10.9
2-oxovalerate
1.9
3-(2-aminobenzoyl)-L-alanine
Hansenula schneggii
-
pH 8.0, 37C
0.2062
3-hydroxykynurenine
in 200 mM phosphate buffer, pH 7.5, at 37C
12.9 - 14.2
3-methyl-2-oxobutanoate
3.3
4-methyl-2-oxopentanoate
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
0.4
alpha-oxo-gamma-methiol-butyric acid
-
in 100 mM boric acid buffer, pH 9.0, at 45C
1.2
beta-phenylpyruvate
0.4 - 18
glyoxylate
1 - 1.6
hydroxyphenylpyruvate
0.5 - 1.4
indo-3-pyruvate
3.6
Indole-3-pyruvate
-
pH 7.5, 38C
1.36 - 6.3
L-3-hydroxykynurenine
6.2 - 246
L-alanine
1.4 - 23.1
L-asparagine
0.7 - 1.3
L-cysteine
5.6
L-glutamate
-
pH 8.0, 37C, alpha-aminoadipate aminotransferase activity
0.7 - 3.8
L-glutamine
1.5 - 5.4
L-histidine
0.083 - 27
L-kynurenine
5 - 34.5
L-leucine
0.9 - 6.4
L-methionine
1.1 - 3.5
L-phenylalanine
3 - 32.7
L-serine
1.2 - 12.9
L-tryptophan
0.9 - 3.2
L-tyrosine
2.7
L-Vinylglycine
Hansenula schneggii
-
pH 8.0. 25C
2.4 - 3.2
Mercaptopyruvate
0.9 - 16.8
oxaloacetate
0.6 - 1.8
phenylpyruvate
0.00085 - 0.0013
pyridoxal 5'-phosphate
0.0032
pyridoxamine 5'-phosphate
Hansenula schneggii
-
pH 8.0, 37C
0.0175 - 12.1
pyruvate
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
38.3 - 317
2-aminobutanoate
0.16 - 4.27
2-oxo-4-methylthiobutyrate
4.84
2-oxoadipate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
234
2-oxobutanoate
Homo sapiens
-
pH 7.5, 45C
0.0018 - 3.49
2-oxobutyrate
4.83
2-oxocaproate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
0.41 - 2.59
2-oxocaproic acid
0.37 - 7.67
2-oxoglutarate
296
2-oxoisocaproate
Homo sapiens
-
pH 7.5, 45C
0.74
2-oxoisocaproic acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
0.13 - 2.7
2-oxovalerate
65.12
3-hydroxykynurenine
Haemaphysalis longicornis
B1B6U5
in 200 mM phosphate buffer, pH 7.5, at 37C
0.92
3-methyl-2-oxobutanoate
2.51
4-methyl-2-oxopentanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
1.02
alpha-oxo-gamma-methiol-butyric acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
0.19 - 263
glyoxylate
0.39 - 1.48
hydroxyphenylpyruvate
1.03 - 1.49
indo-3-pyruvate
0.49
Indole-3-pyruvate
Mus musculus
-
pH 7.5, 38C
1.53 - 1540
L-alanine
2.93 - 230
L-asparagine
1.3 - 206
L-cysteine
2.27 - 561
L-glutamine
2 - 168
L-histidine
1.8 - 201
L-kynurenine
339 - 758
L-leucine
2.43 - 215
L-methionine
2.7 - 283
L-phenylalanine
2.17 - 345
L-serine
3.57 - 283
L-tryptophan
1.03 - 139
L-tyrosine
0.44 - 234
Mercaptopyruvate
0.065 - 143
oxaloacetate
0.63 - 110
phenylpyruvate
0.36 - 29
pyruvate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07 - 1.75
2-oxo-4-methylthiobutyrate
0.23
2-oxoadipate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
1184
0.02 - 8.2
2-oxobutyrate
3.13
2-oxocaproate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
3676
0.04 - 5.18
2-oxocaproic acid
0.045 - 6.24
2-oxoglutarate
0.14
2-oxoisocaproic acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
27233
0.01 - 2.25
2-oxovalerate
315
3-hydroxykynurenine
Haemaphysalis longicornis
B1B6U5
in 200 mM phosphate buffer, pH7.5, at 37C
2461
0.07
3-methyl-2-oxobutanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
829
0.065
3-methyl-2-oxopentanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
1133
0.76
4-methyl-2-oxopentanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
722
36.8
alpha-keto-gamma-methylthiobutyrate
Haemaphysalis longicornis
B1B6U5
in 200 mM phosphate buffer, pH7.5, at 37C
177096
2.54
alpha-oxo-gamma-methiol-butyric acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
27232
0.05 - 6.78
glyoxylate
0.26 - 1.48
hydroxyphenylpyruvate
1.08 - 2.07
indo-3-pyruvate
0.13
Indole-3-pyruvate
Mus musculus
-
pH 7.5, 38C
2408
0.25
L-alanine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
103
2.095
L-asparagine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
266
1.86
L-cysteine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
74
3.24
L-glutamine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
102
2.86
L-histidine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
349
1.53 - 500
L-kynurenine
2.7
L-methionine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
88
2.45
L-phenylalanine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
104
0.72
L-serine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
95
0.5
L-tryptophan
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
119
0.38
L-tyrosine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
109
0.14 - 1.37
Mercaptopyruvate
0.09 - 0.75
oxaloacetate
0.96 - 4.22
phenylpyruvate
0.04 - 116
pyruvate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02011
2-oxoglutarate
-
pH 7.5, 25C
13
adipate
Hansenula schneggii
-
-
0.013
cysteine sulfinate
-
17.2 mM dissociation constant for dissociation of the inhibitor from the enzyme substrate complex, pH 7.5, 25C
9.5
pimelate
Hansenula schneggii
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00091
(S)-(-)-9-(4-aminopiperazin-1-yl)-8-fluoro-3-methyl-6-oxo-2,3-dihydro-6H-1-oxa-3a-azaphenalene-5-carboxylic acid
Homo sapiens
-
0.0015 mM or the two enantiomers, pH 8.0, 37C
0.293
3-(2-carboxyethyl)-5-chloro-1H-indole-2-carboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.666
3-(2-carboxyethyl)-5-nitro-1H-indole-2-carboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.02
5-(2-(3-chlorophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.021
5-(2-(4-bromophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.02
5-(2-(4-chlorophenyl) hydrazono)-6-ethoxy-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.271
5-bromo-3-(2-carboxyethyl)-1H-indole-2-carboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.034
6-ethoxy-5-(2-(4-iodophenyl)hydrazono)-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.043
6-ethoxy-5-(2-(4-methoxyphenyl)hydrazono)-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.179
6-ethoxy-5-(2-(4-nitrophenyl)hydrazono)-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.033
6-ethoxy-6-oxo-5-(2-p-tolylhydrazono)hexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.002
cysteine sulfinate
Homo sapiens
-
pH 7.5, 25C
0.14
Indole-3-propionic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.17
Hansenula schneggii
-
-
16.9
Hansenula schneggii
-
-
71.7
-
pH 7.0, 55C
additional information
-
prawns at intermoult show significant higher KAT activity compared to prawns from premoult
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
-
KAT II from brain
7
-
KAT II
7 - 9
-
KAT IV
7 - 7.5
-
KAT II
7.4 - 10
-
KAT I
8.5
optima at pH 8.5 and 10.0
8.8
-
total placental KAT activity
9 - 10
-
KAT III
9 - 9.5
-
KAT I
9.5 - 10
-
optimal pH range of human brain and placenta KAT I
9.6 - 10
-
KAT I from brain, sharp optimum
9.8
-
placental KAT I activity
10
optima at pH 8.5 and 10.0
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 9.2
-
approx. half-maximal activity at pH 6.2, approx. 60% of maximal activity at pH 9.2
6.5 - 8.8
-
maximal activity at pH 6.5, approx. half-maximal activity at pH 8.8
7 - 9
-
-
7.5 - 9
-
-
8 - 11
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 60
-
-