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Literature summary for 2.6.1.7 extracted from
- Purification, characterization and identification of rat liver mitochondrial kynurenine aminotransferase with alpha-aminoadipate aminotransferase (1983), Biochim. Biophys. Acta, 743, 323-330.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Aminoadipate | - |
Rattus norvegicus |  |
| 2-oxoadipate | - |
Rattus norvegicus | |
| 2-oxoglutarate | substrate inhibition | Rattus norvegicus | |
| 4-chloromercuribenzoate | - |
Rattus norvegicus | |
| adipate | strong inhibition | Rattus norvegicus | |
| anthranilate | weak inhibition | Rattus norvegicus | |
| Co2+ | - |
Rattus norvegicus | |
| Cu2+ | - |
Rattus norvegicus | |
| Fe3+ | - |
Rattus norvegicus | |
| glutamate | weak inhibition | Rattus norvegicus | |
| Hg2+ | strong inhibition | Rattus norvegicus | |
| Kynurenate | weak inhibition | Rattus norvegicus | |
| L-kynurenine | substrate inhibition above 6 mM | Rattus norvegicus | |
| additional information | not inhibited by glycerol, DTT, up to 10%, tryptophan, 3-hydroxyanthranilate, picolinate, NAD+, NADH, NADP+, NADPH, serotonin, tryptamine, 5-hydroxytryptophan, indole-3-acetate, organic acids of tricarboxylic acid cycle, glyoxylate, pyruvate, glutamine, Mg2+, Ca2+, Mn2+, monovalent cations; not inhibited by quinolinate | Rattus norvegicus | |
| nicotinate | weak inhibition | Rattus norvegicus | |
| xanthurenate | weak inhibition | Rattus norvegicus | |
| Zn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
2-oxoadipate | - |
Rattus norvegicus | |
| 0.013 | - |
2-oxoglutarate | cosubstrate hydroxykynurenine | Rattus norvegicus | |
| 0.02 | - |
2-oxoglutarate | cosubstrate L-kynurenine | Rattus norvegicus | |
| 4.3 | - |
L-kynurenine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | - |
Rattus norvegicus | 5743 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49500 | - |
2 * 49500, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.13 | - |
hydroxykynurenine | Rattus norvegicus |
| 1.5 | - |
L-kynurenine | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 10 mM phosphate buffer, pH 7, 50% glycerol, at least 2 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-3-hydroxykynurenine + 2-oxoglutarate | - |
Rattus norvegicus | xanthurenic acid + L-glutamate | - |
ir | |
| L-kynurenine + 2-oxoglutarate | best substrates | Rattus norvegicus | kynurenic acid + L-glutamate + H2O | - |
ir | |
| L-kynurenine + 2-oxoglutarate | best substrates | Rattus norvegicus Wistar | kynurenic acid + L-glutamate + H2O | - |
ir | |
| L-kynurenine + 2-oxohexanedioate | - |
Rattus norvegicus | kynurenic acid + 2-aminohexanedioate + H2O | - |
? | |
| L-kynurenine + 2-oxohexanedioate | - |
Rattus norvegicus Wistar | kynurenic acid + 2-aminohexanedioate + H2O | - |
? | |
| L-kynurenine + glyoxylate | poor substrate | Rattus norvegicus | kynurenic acid + glycine + H2O | - |
? | |
| L-kynurenine + glyoxylate | poor substrate | Rattus norvegicus Wistar | kynurenic acid + glycine + H2O | - |
? | |
| L-kynurenine + oxaloacetate | less effective than 2-oxoglutarate | Rattus norvegicus | kynurenic acid + L-aspartate + H2O | - |
? | |
| L-kynurenine + oxaloacetate | less effective than 2-oxoglutarate | Rattus norvegicus Wistar | kynurenic acid + L-aspartate + H2O | - |
? | |
| L-kynurenine + pyruvate | less effective than 2-oxoglutarate | Rattus norvegicus | kynurenic acid + L-alanine + H2O | - |
? | |
| L-kynurenine + pyruvate | less effective than 2-oxoglutarate | Rattus norvegicus Wistar | kynurenic acid + L-alanine + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 49500, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme from mitochondria may be identical with EC 2.6.1.39 | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
in 20% glycerol, 0.1 mM DTT, 2 mM pyridoxal phosphate, 25 mM imidazole-HCl buffer, pH 7, 30 min stable | Rattus norvegicus |
| 70 | - |
20 min, inactivation | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
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