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Literature summary for 2.6.1.7 extracted from

  • Okada, K.; Angkawidjaja, C.; Koga, Y.; Takano, K.; Kanaya, S.
    Characteristic features of kynurenine aminotransferase allosterically regulated by (alpha)-ketoglutarate in cooperation with kynurenine (2012), PLoS ONE, 7, e40307.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.7

Activating Compound

Activating Compound Comment Organism Structure
L-kynurenine allosterically regulated Pyrococcus horikoshii

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21-CodonPlus (DE3) Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor diffusion method Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate inhibited by high concentrations, allosterically regulated Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00173
-
 2-oxobutyrate 0.1 mM L-kynurenine, pH 7.5, 25°C Pyrococcus horikoshii
0.00241
-
 2-oxo-4-methylthiobutyrate 0.1 mM L-kynurenine, pH 7.5, 25°C Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE, crystal structure Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-
Pyrococcus horikoshii OT-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Pyrococcus horikoshii

Storage Stability

Storage Stability Organism
full activity after 12 month at -80°C, 50 mM HEPES/NaOH, pH 7.5, 100 mM NaCl Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-kynurenine + 2-oxo-4-methylthiobutyrate
-
 Pyrococcus horikoshii kynurenate + L-methionine
-
 ?
L-kynurenine + 2-oxo-4-methylthiobutyrate
-
 Pyrococcus horikoshii OT-3 kynurenate + L-methionine
-
 ?
L-kynurenine + 2-oxobutyrate
-
 Pyrococcus horikoshii kynurenate + ?
-
 ?
L-kynurenine + 2-oxobutyrate
-
 Pyrococcus horikoshii OT-3 kynurenate + ?
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Pyrococcus horikoshii kynurenate + L-glutamate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Pyrococcus horikoshii OT-3 kynurenate + L-glutamate + H2O
-
 ?
L-kynurenine + oxaloacetate
-
 Pyrococcus horikoshii kynurenate + L-aspartate + H2O
-
 ?
L-kynurenine + oxaloacetate
-
 Pyrococcus horikoshii OT-3 kynurenate + L-aspartate + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 48000, SDS-PAGE, crystal structure Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
kynurenine aminotransferase
-
 Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pyrococcus horikoshii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02011
-
 2-oxoglutarate pH 7.5, 25°C Pyrococcus horikoshii