Information on EC 2.1.2.9 - methionyl-tRNA formyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.1.2.9
-
RECOMMENDED NAME
GeneOntology No.
methionyl-tRNA formyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
10-formyltetrahydrofolate + L-methionyl-tRNAfMet = tetrahydrofolate + N-formylmethionyl-tRNAfMet
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
formyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Aminoacyl-tRNA biosynthesis
-
One carbon pool by folate
-
SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate:L-methionyl-tRNA N-formyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
FMT
-
-
-
-
formylmethionyl-transfer ribonucleic synthetase
-
-
-
-
methionyl ribonucleic formyltransferase
-
-
-
-
methionyl-transfer ribonucleic transformylase
-
-
-
-
methionyl-transfer RNA transformylase
-
-
-
-
methionyl-tRNA formyltransferase
-
-
methionyl-tRNA formyltransferase
-
-
methionyl-tRNA formyltransferase
-
-
methionyl-tRNA formyltransferase
-
-
-
methionyl-tRNA Met formyltransferase
-
-
-
-
methionyl-tRNA transformylase
-
-
-
-
methionyl-tRNA-Formyltransferase
-
-
methyltransferase, methionyl-transfer ribonucleate
-
-
-
-
mitochondrial methionyl-tRNA formyltransferase
-
-
MTF
-
-
-
-
MTFMT
-
-
N10-formyltetrahydrofolic-methionyl-transfer ribonucleic transformylase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9015-76-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Enterococcus faecalis R
strain R
-
-
Manually annotated by BRENDA team
strain A19
-
-
Manually annotated by BRENDA team
strain B
-
-
Manually annotated by BRENDA team
strain CA274; strain DH5AlphaF'; strain JM109
-
-
Manually annotated by BRENDA team
strain CA274; strain JM109; strains DH5Alpha, Novablue(DE3) pLysS
-
-
Manually annotated by BRENDA team
strain JM109; strain XL1-Blue
Uniprot
Manually annotated by BRENDA team
strain K12 EM20031(F32)
-
-
Manually annotated by BRENDA team
strain K12 MO (lot 15-290)
-
-
Manually annotated by BRENDA team
Escherichia coli A19
strain A19
-
-
Manually annotated by BRENDA team
Escherichia coli CA274
strain CA274
-
-
Manually annotated by BRENDA team
Escherichia coli DH5AlphaF
strain DH5AlphaF'
-
-
Manually annotated by BRENDA team
Escherichia coli JM109
strain JM109
-
-
Manually annotated by BRENDA team
Escherichia coli JM109
strain JM109
Uniprot
Manually annotated by BRENDA team
Escherichia coli K12 EM20031(F32)
strain K12 EM20031(F32)
-
-
Manually annotated by BRENDA team
Escherichia coli K12 MO
strain K12 MO (lot 15-290)
-
-
Manually annotated by BRENDA team
Escherichia coli XL1-Blue
strain XL1-Blue
Uniprot
Manually annotated by BRENDA team
strain Z
-
-
Manually annotated by BRENDA team
Euglena gracilis Z
strain Z
-
-
Manually annotated by BRENDA team
strain D243-2B-R1
-
-
Manually annotated by BRENDA team
strains CMY214, W303, YPALS, DBY2057
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae D243-2B-R1
strain D243-2B-R1
-
-
Manually annotated by BRENDA team
possesses a single gene coding for each FMT and peptide deformylase, PDF, respectively
-
-
Manually annotated by BRENDA team
possesses a single gene coding for each FMT and peptide deformylase, PDF, respectively
-
-
Manually annotated by BRENDA team
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
10-Formylaminopterin
-
0.01 mM: 38% inhibition, 0.1 mM: 62% inhibition, competitive
10-formylpteroylglutamic acid
-
0.1 mM: 31% inhibition, 1 mM: 52% inhibition, competitive
5,10-methylenetetrahydrofolate
-
-
5-formyltetrahydrofolate
-
competitive inhibition
5-methyltetrahydrofolate
-
most effective, non-competitive inhibitor
aminopterin
-
1 mM: 26% inhibition, competitive inhibition
reduced aminopterin
-
1 mM, 34% inhibition, competitive inhibition
tetrahydrofolate
-
competitive, multisite inhibition
tetrahydrofolate
-
competitive inhibition
tRNAfMet
-
tRNAfMet from Streptococcus faecalis or E. coli K12, strong inhibition
tRNAfMet
-
non-aminoacylated tRNAfMet is a competitive inhibitor
methotrexate
-
0.01 mM: 30% inhibition, 0.1 mM, 52% inhibition, competitive
additional information
-
inhibition studies, not inhibited by trimethoprim; not inhibited by folic acid
-
additional information
-
not inhibited by folic acid; not inhibited by pteroic acid, aminopterin, methionyl-tRNAmMet and uncharged tRNAmMet
-
additional information
-
E. coli A19 enzyme is not inhibited by uncharged tRNAmMet and tRNAfMet from E. coli K12 or Streptococcus faecalis
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
not stimulated by ATP or GTP
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.045
-
5-formyltetrahydrofolate
-
-
0.003
-
5-methyltetrahydrofolate
-
-
0.0002
-
tetrahydrofolate
-
-
0.024
-
tetrahydrofolate
-
-
0.00018
0.00057
tRNAfMet
-
-
PDB
SCOP
CATH
ORGANISM
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 40000, SDS-PAGE
monomer
-
1 * 32000, SDS-PAGE
monomer
-
1 * 32000
monomer
Escherichia coli K12 EM20031(F32)
-
1 * 32000, SDS-PAGE; 1 * 32000, SDS-PAGE
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystal structure of enzyme complexed with formylmethionyl-tRNAfMet
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
EDTA or 2-mercaptoethanol stabilizes
-
high ionic strength, e.g. 150 mM KCl stabilizes and prevents adsorption to glassware
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Glycerol
-
50%, stable to
Glycerol
Escherichia coli K12 EM20031(F32)
-
50%, stable to
-