Information on EC 2.1.2.9 - methionyl-tRNA formyltransferase

New: Word Map on EC 2.1.2.9
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.1.2.9
-
RECOMMENDED NAME
GeneOntology No.
methionyl-tRNA formyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
10-formyltetrahydrofolate + L-methionyl-tRNAfMet = tetrahydrofolate + N-formylmethionyl-tRNAfMet
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminoacyl-tRNA biosynthesis
-
-
One carbon pool by folate
-
-
SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate:L-methionyl-tRNA N-formyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9015-76-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain R
-
-
Manually annotated by BRENDA team
strain R
-
-
Manually annotated by BRENDA team
strain A19
-
-
Manually annotated by BRENDA team
strain CA274
-
-
Manually annotated by BRENDA team
Escherichia coli DH5-alphaF
strain DH5AlphaF'
-
-
Manually annotated by BRENDA team
Escherichia coli K12 EM20031(F32)
strain K12 EM20031(F32)
-
-
Manually annotated by BRENDA team
strain K12 MO (lot 15-290)
-
-
Manually annotated by BRENDA team
strain XL1-Blue
Uniprot
Manually annotated by BRENDA team
strain Z
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain D243-2B-R1
-
-
Manually annotated by BRENDA team
possesses a single gene coding for each FMT and peptide deformylase, PDF, respectively
-
-
Manually annotated by BRENDA team
possesses a single gene coding for each FMT and peptide deformylase, PDF, respectively
-
-
Manually annotated by BRENDA team
wheat
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O
tetrahydrofolate + N-formylisoleucyl-tRNAfMet
show the reaction diagram
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
show the reaction diagram
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
show the reaction diagram
10-formyltetrahydrofolate + L-phenylalanyl-tRNAfMet + H2O
tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
show the reaction diagram
-
mutant tRNA G34A36 is a good substrate
-
-
?
10-formyltetrahydrofolate + methionyl-tRNA1Val(CAU) + H2O
tetrahydrofolate + N-formylmethionyl-tRNA1Val(CAU)
show the reaction diagram
-
mischarged E. coli tRNA1Val(CAU), less efficient than with methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + methionyl-tRNAmMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAmMet
show the reaction diagram
-
E. coli elongator Met-tRNAmMet, less efficient than with methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O
tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
show the reaction diagram
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
show the reaction diagram
10-formyltetrahydropteroyltriglutamate + L-methionyl-tRNAfMet + H2O
tetrahydropteroyltriglutamate + N-formylmethionyl-tRNAfMet
show the reaction diagram
-
10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can act as donor
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
show the reaction diagram
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10-formyltetrahydrofolate
tetrahydrofolate
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
can partially replace Mg2+
spermine
-
activates, can partially replace Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10-Formylaminopterin
-
0.01 mM: 38% inhibition, 0.1 mM: 62% inhibition, competitive
10-formylpteroylglutamic acid
-
0.1 mM: 31% inhibition, 1 mM: 52% inhibition, competitive
5,10-methylenetetrahydrofolate
-
-
5-formyltetrahydrofolate
-
competitive inhibition
5-methyltetrahydrofolate
-
most effective, non-competitive inhibitor
aminopterin
-
1 mM: 26% inhibition, competitive inhibition
methotrexate
-
0.01 mM: 30% inhibition, 0.1 mM, 52% inhibition, competitive
reduced aminopterin
-
1 mM, 34% inhibition, competitive inhibition
tetrahydrofolate
tRNAfMet
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006 - 0.0135
10-formyltetrahydrofolate
0.011 - 0.016
10-formyltetrahydropteroyltriglutamate
-
-
0.00004
bovine mitochondrial methionyl-tRNA
-
-
-
0.0003
E. coli isoleucyl-tRNAfMet
0.01
E. coli methionyl-tRNA1Val(CAU)
-
above
-
0.0001 - 0.00112
E. coli methionyl-tRNAfMet
0.000097 - 0.01
E. coli methionyl-tRNAmMet
0.0005 - 0.0014
E. coli valyl-tRNAfMet
0.00035 - 0.0096
methionyl-tRNAfMet
0.01
methionyl-tRNAMet
-
-
-
0.0013
phenylalanyl-tRNAfMet
-
-
0.000086 - 0.00119
tRNAfMet
0.0014
valyl-tRNAfMet
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0005
E. coli isoleucyl-tRNAfMet
Saccharomyces cerevisiae
-
-
-
0.03
E. coli methionyl-tRNA1Val(CAU)
Bos taurus
-
above
-
0.13 - 11.3
E. coli methionyl-tRNAfMet
0.04
E. coli methionyl-tRNAmMet
Bos taurus
-
above
-
0.009
E. coli valyl-tRNAfMet
Saccharomyces cerevisiae
-
-
-
0.2 - 46.3
methionyl-tRNAfMet
additional information
additional information
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
5-formyltetrahydrofolate
-
-
0.003
5-methyltetrahydrofolate
-
-
0.0002 - 0.024
tetrahydrofolate
0.00018 - 0.00057
tRNAfMet
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.64 - 0.73
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
good activity between
6.8 - 8.4
-
about 60% of maximal activity at pH 6.8 and 8.4
7
-
below: rapid decrease in reaction rate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
higher specific activity in isolated chloroplasts than in the remainder of the leaf extract
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
high speed equilibrium sedimentation
29900
-
sucrose density gradient centrifugation
30000
-
sedimentation equilibrium method
33000
-
sedimentation equilibrium method, gel filtration, small-angle neutron scattering
34000
-
gel filtration, small-angle neutron scattering
38000
-
gel filtration
45000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 40000, SDS-PAGE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of enzyme complexed with formylmethionyl-tRNAfMet
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
EDTA or 2-mercaptoethanol stabilizes
-
high ionic strength, e.g. 150 mM KCl stabilizes and prevents adsorption to glassware
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Glycerol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, several months, stable
-
-20C, 1 month, 30% loss of activity
-
-20C, 20 mM imidazole, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, stable
-
-20C, 20 mM imidazole, pH 7.5, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, 2-4 months, stable
-
-20C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, 6 months, stable
-
-20C, longer than 4 months, stable
-
25C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 150 mM KCl, 2 days, stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
100-300fold, partial purification
-
10000fold purification; affinity chromatography on Sepharose-bound tRNA
-
1000fold purification from autotrophic cells, 650fold from chloroplasts
-
14fold purification of recombinant wild-type enzyme, overexpressed in Escherichia coli Novablue, purification of His-tagged G178E mutant enzyme
-
affinity chromatography on Sepharose-bound tRNA
-
over 1500fold purification
-
purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3)
-
purification of His-tagged wild-type and C-terminal deletion mutant recombinant enzymes, expressed in Escherichia coli
purification of His6-tagged wild-type and mutant recombinant enzymes, expressed in Escherichia coli BL21(DE3)
-
purification of recombinant enzyme, expressed in Escherichia coli
-
purification of wild-type and of R42K, R42L and G41R/R42L mutant recombinant enzymes, expressed in Escherichia coli JM109
-
purification of wild-type enzyme and G41R, G41K mutant enzymes
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning and expression of the gene encoding mitochondrial enzyme in Escherichia coli Pal13Tr-pMAF and in yeast, several strains lacking gene exhibit in minimal medium a thermosensitive respiratory growth and a slower growth rate
-
cloning of the fmt gene and expression of wild-type and mutant genes in Escherichia coli BL21(DE3) as His6-tagged proteins
-
expression in Escherichia coli
-
expression of wild-type and of R42K, R42L and G41R/R42L mutant enzymes in Escherichia coli JM109 as His6-tagged proteins
-
fmt gene encoding enzyme is cloned
fmt gene encoding MTF is cloned and sequenced, expression in Escherichia coli JM109 as His6-tagged enzyme
-
gene encoding mitochondrial enzyme is cloned and expressed in Escherichia coli BL21(DE3) as His-tagged enzyme
-
gene encoding MTF is cloned and sequenced, expression of wild-type and mutant enzymes in Escherichia coli
possesses a single gene coding for each FMT and peptide deformylase, PDF, respectively, and both gene products are predicted to carry a bipartite targeting signal
-
wild-type and mutant fmt gene encoding MTF is cloned, overexpression of wild-type enzyme in Escherichia coli Novablue
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A55T
-
no suppressor mutation
G178D
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E
G178E
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors
G178K
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
G178L
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
G41Q
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
R38K
-
mutant enzyme with 2.2fold increased Km, no effect on kcat
R38L
-
mutant enzyme with up to 12fold lower specific activity, 6fold increased Km and 5fold decreased kcat
R42K
-
mutant enzyme with lower activity, 16fold increased Km
R42L
-
single mutant and G41R/R42L double mutant with low activity, R42L: 17fold increased Km and 79fold decreased kcat
R42Q
-
mutant enzyme with lower activity, 16-17fold increased Km
A55T
-
no suppressor mutation
-
G178D
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178E
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors
-
G178K
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178L
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G41D
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41K
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41L
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41Q
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41R
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41D
Escherichia coli DH5-alphaF
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41K
Escherichia coli DH5-alphaF
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41L
Escherichia coli DH5-alphaF
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41Q
Escherichia coli DH5-alphaF
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41R
Escherichia coli DH5-alphaF
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
A55T
-
no suppressor mutation
-
G178D
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178E
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors
-
G178K
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178L
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G41D
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41K
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41L
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41Q
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41R
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
D145A
-
mutant enzyme with drastically reduced activity
D145E
-
mutant enzyme with 881fold reduced formylation rate, kinetic parameters
D145N
-
mutant enzyme with 4031fold reduced formylation rate, kinetic parameters
H109A
-
mutant enzyme with 4677fold reduced formylation rate, kinetic parameters
H109K
-
inactive mutant enzyme
H109R
-
mutant enzyme with 7172fold reduced formylation rate, kinetic parameters
K37A
-
mutant enzyme with 3.5fold reduced formylation rate, kinetic parameters
N107A
-
mutant enzyme with 48fold reduced formylation rate, kinetic parameters
R41A
-
mutant enzyme with 470fold reduced formylation rate, kinetic parameters
R41K
-
mutant enzyme with 140fold reduced formylation rate, kinetic parameters
V31A
-
mutant enzyme with 1.7fold reduced formylation rate
del833
-
inactivated methionyl-tRNA formyltransferase
deltafmt1
-
in combination with mutation of AEP3, a gene encodes for a peripheral mitochondrial inner membrane protein, respiratory defect
additional information
Show AA Sequence (10851 entries)
Longer loading times are possible. Please use the Sequence Search for a certain query.