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Literature summary for 2.1.2.9 extracted from

  • Vial, L.; Gomez, P.; Panvert, M.; Schmitt, E.; Blanquet, S.; Mechulam, Y.
    Mitochondrial methionyl-tRNAfMet formyltransferase from Saccharomyces cerevisiae: Gene disruption and tRNA substrate specificity (2003), Biochemistry, 42, 932-939.
    View publication on PubMed
Search for more literature for 2.1.2.9

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of the gene encoding mitochondrial enzyme in Escherichia coli Pal13Tr-pMAF and in yeast, several strains lacking gene exhibit in minimal medium a thermosensitive respiratory growth and a slower growth rate Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Saccharomyces cerevisiae
0.0003
-
 E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae
0.0003
-
 E. coli L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae
0.0005
-
 E. coli L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Saccharomyces cerevisiae initiation of protein synthesis in mitochondria tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 strains CMY214, W303, YPALS, DBY2057
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme, expressed in Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O 250fold reduced catalytic efficiency compared with methionyl-tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates yeast mitochondrial methionyl-tRNAfMet, specific for initiator tRNAs Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O enzyme recognizes nucleotidic features in the acceptor stem of mitochondrial initiator tRNA, recognition mechanism Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O initiation of protein synthesis in mitochondria Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O 25fold reduced catalytic efficiency compared with methionyl-tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
additional information mutant tRNAs as poor substrates Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Saccharomyces cerevisiae
0.0005
-
 L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae
0.009
-
 L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae
0.13
-
 E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae