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Literature summary for 2.1.2.9 extracted from

  • Mayer, C.; RajBhandary, U.L.
    Conformational change of Escherichia coli initiator methionyl-tRNAfMet upon binding to methionyl-tRNA formyl transferase (2002), Nucleic Acids Res., 30, 2844-2850.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of enzyme complexed with formylmethionyl-tRNAfMet Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli formyl group provides a positive determinant for IF2, which can select the initiator fMet-tRNA from other tRNAs and a negative determinant, which blocks the binding of EF-Tu to the initiator tRNA tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli P23882
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-

Purification (Commentary)

Purification (Comment) Organism
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Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O determinants for formylation are located in the acceptor stem and D-stem of the initiator tRNA, enzyme binds to the tRNA substrate in an induced fit mechanism, tRNA also undergoes induced structural changes during its binding to enzyme Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O tRNA substrate binding Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O important role of a 16-amino acid insertion loop in enzyme for tRNA recognition Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formyl group provides a positive determinant for IF2, which can select the initiator fMet-tRNA from other tRNAs and a negative determinant, which blocks the binding of EF-Tu to the initiator tRNA Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
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?
additional information no formylation of methionyl-tRNAmMet Escherichia coli ?
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?
additional information enzyme structure Escherichia coli ?
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?