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Literature summary for 2.1.2.9 extracted from

  • Ramesh, V.; Gite, S.; Li, Y.; RajBhandary, U.L.
    Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase: Role of a 16-amino acid insertion module in initiator tRNA recognition (1997), Proc. Natl. Acad. Sci. USA, 94, 13524-13529.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.2.9

Cloned(Commentary)

Cloned (Comment) Organism
fmt gene encoding MTF is cloned and sequenced, expression in Escherichia coli JM109 as His6-tagged enzyme Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
G41D no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274 Escherichia coli
G41K suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem Escherichia coli
G41L no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274 Escherichia coli
G41Q no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274 Escherichia coli
G41R suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information values for wild-type and mutant enzyme with wild-type and mutant initiator tRNAs Escherichia coli
0.00056
-
 L-methionyl-tRNAfMet
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli DH5-alphaF involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli JM109 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli CA274 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 strain DH5AlphaF'
-
Escherichia coli CA274
-
-
-
Escherichia coli DH5-alphaF
-
 strain DH5AlphaF'
-
Escherichia coli JM109
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of wild-type enzyme and G41R, G41K mutant enzymes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O recognition mechanism of methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O important role of a 16-amino acid insertion loop in enzyme for tRNA recognition Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O recognition mechanism of methionyl-tRNAfMet Escherichia coli DH5-alphaF tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O important role of a 16-amino acid insertion loop in enzyme for tRNA recognition Escherichia coli DH5-alphaF tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli DH5-alphaF tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O recognition mechanism of methionyl-tRNAfMet Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O important role of a 16-amino acid insertion loop in enzyme for tRNA recognition Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O recognition mechanism of methionyl-tRNAfMet Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O important role of a 16-amino acid insertion loop in enzyme for tRNA recognition Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
additional information mutant tRNAs as poor substrates Escherichia coli ?
-
 ?
additional information enzyme structure Escherichia coli ?
-
 ?
additional information mutant tRNAs as poor substrates Escherichia coli DH5-alphaF ?
-
 ?
additional information enzyme structure Escherichia coli DH5-alphaF ?
-
 ?
additional information mutant tRNAs as poor substrates Escherichia coli JM109 ?
-
 ?
additional information enzyme structure Escherichia coli JM109 ?
-
 ?
additional information mutant tRNAs as poor substrates Escherichia coli CA274 ?
-
 ?
additional information enzyme structure Escherichia coli CA274 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information values for wild-type and mutant enzyme with wild-type and mutant initiator tRNAs Escherichia coli
41.5
-
 L-methionyl-tRNAfMet
-
 Escherichia coli