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Literature summary for 2.1.2.9 extracted from

  • Kahn, D.; Fromant, M.; Fayat, G.; Dessen, P.; Blanquet, S.
    Methionyl-transfer-RNA transformylase from Escherichia coli. Purification and characterisation (1980), Eur. J. Biochem., 105, 489-497.
    View publication on PubMed
Search for more literature for 2.1.2.9

General Stability

General Stability Organism
high ionic strength, e.g. 150 mM KCl stabilizes and prevents adsorption to glassware Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 L-methionyl-tRNAfMet
-
 Escherichia coli
0.0135
-
 10-formyltetrahydrofolate
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requirement, maximum activity at 10 mM Mg2+ and 150 mM KCl Escherichia coli
Mg2+ requirement Escherichia coli
Mg2+ maximum activity at 10 mM Mg2+ and 150 mM KCl Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 sedimentation equilibrium method Escherichia coli
32000
-
 1 * 32000, SDS-PAGE Escherichia coli
34000
-
 gel filtration, small-angle neutron scattering Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K12 EM20031(F32)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
10000fold purification Escherichia coli
affinity chromatography on Sepharose-bound tRNA Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.4
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, 6 months, stable Escherichia coli
25°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 150 mM KCl, 2 days, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O affinity of tRNAfMet for enzyme is improved by aminoacylation Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O affinity of tRNAfMet for enzyme is improved by aminoacylation Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
additional information no formyl group donor: 5-formyltetrahydrofolate Escherichia coli ?
-
 ?
additional information no formylation of methionyl-tRNAmMet Escherichia coli ?
-
 ?
additional information no formyl group donor: 5,10-methenyltetrahydrofolate Escherichia coli ?
-
 ?
additional information no formyl group donor: 5-formyltetrahydrofolate Escherichia coli K12 EM20031(F32) ?
-
 ?
additional information no formylation of methionyl-tRNAmMet Escherichia coli K12 EM20031(F32) ?
-
 ?
additional information no formyl group donor: 5,10-methenyltetrahydrofolate Escherichia coli K12 EM20031(F32) ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 L-methionyl-tRNAfMet + 10-formyltetrahydrofolate Escherichia coli

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli
tetrahydrofolate
-
 Escherichia coli