Cloned (Comment) | Organism |
---|---|
gene encoding mitochondrial enzyme is cloned and expressed in Escherichia coli BL21(DE3) as His-tagged enzyme | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of the formylation of various mutant methionyl-tRNAs | Bos taurus | |
0.0003 | - |
E. coli L-isoleucyl-tRNAfMet | - |
Bos taurus | |
0.0005 | - |
E. coli L-methionyl-tRNAfMet | - |
Bos taurus | |
0.0014 | - |
E. coli L-valyl-tRNAfMet | - |
Bos taurus | |
0.01 | - |
E. coli L-methionyl-tRNAmMet | above | Bos taurus | |
0.01 | - |
E. coli L-methionyl-tRNA1Val(CAU) | above | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | translational initiation in mitochondria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3) | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet | - |
Bos taurus | tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNA1Val(CAU) | - |
Bos taurus | tetrahydrofolate + E. coli N-formylisoleucyl-tRNA1Val(CAU) | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet | - |
Bos taurus | tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet | - |
Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet | - |
Bos taurus | tetrahydrofolate + E. coli N-formylmethionyl-tRNAmMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet | - |
Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAmMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet | - |
Bos taurus | tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Bos taurus | tetrahydrofolate + N-formylisoleucyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O | less efficient than with methionyl-tRNAfMet | Bos taurus | tetrahydrofolate + N-formylisoleucyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | enzyme preferentially recognizes the methionyl moiety of its tRNA substrate, small importance of the tRNA acceptor stem for recognition, E. coli tRNA mutants as substrates | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | broader substrate specificity than that of E. coli enzyme | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | recognition mechanism of methionyl-tRNAfMet | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + methionyl-tRNA1Val(CAU) + H2O | mischarged E. coli tRNA1Val(CAU), less efficient than with methionyl-tRNAfMet | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNA1Val(CAU) | - |
? | |
10-formyltetrahydrofolate + methionyl-tRNAmMet + H2O | E. coli elongator Met-tRNAmMet, less efficient than with methionyl-tRNAfMet | Bos taurus | tetrahydrofolate + N-formylmethionyl-tRNAmMet | - |
? | |
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Bos taurus | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | less efficiency than with methionyl-tRNAfMet | Bos taurus | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
additional information | no formylation of Val-tRNA1Met | Bos taurus | ? | - |
? | |
additional information | translational initiation in mitochondria | Bos taurus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of the formylation of various mutant methionyl-tRNAs | Bos taurus | |
0.03 | - |
E. coli L-methionyl-tRNA1Val(CAU) | above | Bos taurus | |
0.04 | - |
E. coli L-methionyl-tRNAmMet | above | Bos taurus | |
0.93 | - |
E. coli L-methionyl-tRNAfMet | - |
Bos taurus |